VPS11 (NM_001290185) Human Tagged ORF Clone

CAT#: RG239795

VPS11 (GFP-tagged) - Human vacuolar protein sorting 11 homolog (S. cerevisiae) (VPS11), transcript variant 2

Specifications

Product Data

• Tag: TurboGFP
• Symbol: VPS11
• Synonyms: END1; HLD12; hVPS11; PEP5; RNF108
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG239795 representing NM_001290185.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGAAATCTGTTTGTCGGAGGGGTCCGTGCCGCGCGCCTCTTTGGTTTAGCTGGTCATCCAGAGTTGTT
  CTGTGGTCTACGGGAAGAAAGAAGGAAGTCCATCTCCTAACTTGTTATCAACTGAGCAATCCTGGGAGG
  CTCCTTGACTACCCTGCACATATGGAAGGCCAGATCTGGTTCTTGCCACGTTCCCTACAGCTTACAGGC
  TTCCAAGCCTACAAACTACGGGTGACACACCTGTACCAACTGAAGCAGCACAATATTCTGGCATCTGTT
  GGAGAAGATGAAGAGGGCATCAACCCCTTGGTTAAGATCTGGAACCTGGAGAAGAGAGATGGTGGCAAT
  CCACTCTGCACTCGAATCTTCCCTGCTATTCCAGGAACAGAGCCAACTGTTGTATCTTGTTTGACTGTC
  CATGAAAATCTCAACTTTATGGCCATTGGTTTCACAGATGGCAGTGTTACATTGAACAAAGGAGACATC
  ACCCGGGACCGGCATAGCAAGACCCAGATTTTGCACAAGGGCAACTATCCTGTAACTGGATTGGCCTTT
  CGCCAAGCAGGAAAGACCACTCACTTGTTTGTTGTGACAACAGAGAACGTCCAGTCCTATATAGTTTCT
  GGAAAAGACTACCCTCGCGTGGAGTTGGACACCCATGGTTGTGGCCTGCGCTGCTCAGCCCTAAGTGAC
  CCTTCTCAGGACCTGCAGTTCATTGTGGCCGGGGATGAGTGTGTCTACTTGTACCAGCCTGATGAACGT
  GGGCCCTGCTTCGCCTTTGAGGGCCATAAGCTCATTGCCCACTGGTTTAGAGGCTACCTTATCATTGTC
  TCCCGTGACCGGAAGGTTTCTCCCAAGTCAGAGTTTACCAGCAGGGATTCACAGAGCTCCGACAAGCAG
  ATTCTAAACATCTATGACCTGTGCAACAAGTTCATAGCCTATAGCACCGTCTTTGAGGATGTAGTGGAT
  GTGCTTGCTGAGTGGGGCTCCCTGTACGTGCTGACGCGGGATGGGCGGGTCCACGCACTGCAGGAGAAG
  GACACACAGACCAAACTGGAGATGCTGTTTAAGAAGAACCTATTTGAGATGGCGATTAACCTTGCCAAG
  AGCCAGCATCTGGACAGTGATGGGCTGGCCCAGATTTTCATGCAGTATGGAGACCATCTCTACAGCAAG
  GGCAACCACGATGGGGCTGTCCAGCAATATATCCGAACCATTGGAAAGTTGGAGCCATCCTACGTGATC
  CGCAAGTTTCTGGATGCCCAGCGCATTCACAACCTGACTGCCTACCTGCAGACCCTGCACCGACAATCC
  CTGGCCAATGCCGACCATACCACCCTGCTCCTCAACTGCTATACCAAGCTCAAGGACAGCTCGAAGCTG
  GAGGAGTTCATCAAGAAAAAGAGTGAGAGTGAAGTCCACTTTGATGTGGAGACAGCCATCAAGGTCCTC
  CGGCAGGCTGGCTACTACTCCCATGCCCTGTATCTGGCGGAGAACCATGCACATCATGAGTGGTACCTG
  AAGATCCAGCTAGAAGACATTAAGAATTATCAGGAAGCCCTTCGATACATCGGCAAGCTGCCTTTTGAG
  CAGGCAGAGAGCAACATGAAGCGCTACGGCAAGATCCTCATGCACCACATACCAGAGCAGACAACTCAG
  TTGCTGAAGGGACTTTGTACTGATTATCGGCCCAGCCTCGAAGGCCGCAGCGATAGGGAGGCCCCAGGC
  TGCAGGGCCAACTCTGAGGAGTTCATCCCCATCTTTGCCAATAACCCGCGAGAGCTGAAAGCCTTCCTA
  GAGCACATGAGTGAAGTGCAGCCAGACTCACCCCAGGGGATCTACGACACACTCCTTGAGCTGCGACTG
  CAGAACTGGGCCCACGAGAAGGATCCACAGGTCAAAGAGAAGCTTCACGCAGAGGCCATTTCCCTGCTG
  AAGAGTGGTCGCTTCTGCGACGTCTTTGACAAGGCCCTGGTCCTGTGCCAGATGCACGACTTCCAGGAT
  GGTGTCCTTTACCTTTATGAGCAGGGGAAGCTGTTCCAGCAGATCATGCACTACCACATGCAGCACGAG
  CAGTACCGGCAGGTCATCAGCGTGTGTGAGCGCCATGGGGAGCAGGACCCCTCCTTGTGGGAGCAGGCC
  CTCAGCTACTTCGCTCGCAAGGAGGAGGACTGCAAGGAGTATGTGGCAGCTGTCCTCAAGCATATCGAG
  AACAAGAACCTCATGCCACCTCTTCTAGTGGTGCAGACCCTGGCCCACAACTCCACAGCCACACTCTCC
  GTCATCAGGGACTACCTGGTCCAAAAACTACAGAAACAGAGCCAGCAGATTGCACAGGATGAGCTGCGG
  GTGCGGCGGTACCGAGAGGAGACCACCCGTATCCGCCAGGAGATCCAAGAGCTCAAGGCCAGTCCTAAG
  ATTTTCCAAAAGACCAAGTGCAGCATCTGTAACAGTGCCTTGGAGTTGCCCTCAGTCCACTTCCTGTGT
  GGCCACTCCTTCCACCAACACTGCTTTGAGAGTTACTCGGAAAGTGATGCTGACTGCCCCACCTGCCTC
  CCTGAAAACCGGAAGGTCATGGATATGATCCGGGCCCAGGAACAGAAACGAGATCTCCATGATCAATTC
  CAGCATCAGCTCAAGTGCTCCAATGACAGCTTTTCTGTGATTGCTGACTACTTTGGCAGAGGTGTTTTC
  AACAAATTGACTCTGCTGACCGACCCTCCCACAGCCAGACTGACCTCCAGCCTGGAGGCTGGGCTGCAA
  CGCGACCTACTCATGCACTCCAGGAGGGGCACT
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG239795
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MKSVCRRGPCRAPLWFSWSSRVVLWSTGRKKEVHLLTCYQLSNPGRLLDYPAHMEGQIWFLPRSLQLTG
  FQAYKLRVTHLYQLKQHNILASVGEDEEGINPLVKIWNLEKRDGGNPLCTRIFPAIPGTEPTVVSCLTV
  HENLNFMAIGFTDGSVTLNKGDITRDRHSKTQILHKGNYPVTGLAFRQAGKTTHLFVVTTENVQSYIVS
  GKDYPRVELDTHGCGLRCSALSDPSQDLQFIVAGDECVYLYQPDERGPCFAFEGHKLIAHWFRGYLIIV
  SRDRKVSPKSEFTSRDSQSSDKQILNIYDLCNKFIAYSTVFEDVVDVLAEWGSLYVLTRDGRVHALQEK
  DTQTKLEMLFKKNLFEMAINLAKSQHLDSDGLAQIFMQYGDHLYSKGNHDGAVQQYIRTIGKLEPSYVI
  RKFLDAQRIHNLTAYLQTLHRQSLANADHTTLLLNCYTKLKDSSKLEEFIKKKSESEVHFDVETAIKVL
  RQAGYYSHALYLAENHAHHEWYLKIQLEDIKNYQEALRYIGKLPFEQAESNMKRYGKILMHHIPEQTTQ
  LLKGLCTDYRPSLEGRSDREAPGCRANSEEFIPIFANNPRELKAFLEHMSEVQPDSPQGIYDTLLELRL
  QNWAHEKDPQVKEKLHAEAISLLKSGRFCDVFDKALVLCQMHDFQDGVLYLYEQGKLFQQIMHYHMQHE
  QYRQVISVCERHGEQDPSLWEQALSYFARKEEDCKEYVAAVLKHIENKNLMPPLLVVQTLAHNSTATLS
  VIRDYLVQKLQKQSQQIAQDELRVRRYREETTRIRQEIQELKASPKIFQKTKCSICNSALELPSVHFLC
  GHSFHQHCFESYSESDADCPTCLPENRKVMDMIRAQEQKRDLHDQFQHQLKCSNDSFSVIADYFGRGVF
  NKLTLLTDPPTARLTSSLEAGLQRDLLMHSRRGT
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001290185
• ORF Size: 2793 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.

Reference Data

• RefSeq: NM_001290185.2
• RefSeq Size: 3486 bp
• RefSeq ORF: 2796 bp
• Locus ID: 55823
• Cytogenetics: 11q23.3
• Protein Families: Druggable Genome
• MW: 107.8 kDa
• Gene Summary: Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinct organelles. Genetic studies in yeast have identified more than 40 vacuolar protein sorting (VPS) genes involved in vesicle transport to vacuoles. This gene encodes the human homolog of yeast class C Vps11 protein. The mammalian class C Vps proteins are predominantly associated with late endosomes/lysosomes, and like their yeast counterparts, may mediate vesicle trafficking steps in the endosome/lysosome pathway. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014]