TMEM16B (ANO2) (NM_001278597) Human Tagged ORF Clone

CAT#: RG239867

ANO2 (GFP-tagged) - Human anoctamin 2, calcium activated chloride channel (ANO2), transcript variant 2

Specifications

Product Data

• Tag: TurboGFP
• Symbol: ANO2
• Synonyms: C12orf3; TMEM16B
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG239867 representing NM_001278597.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGCCTGAAGATATACCCCTGCTCCCTGGCTCCCCACGCCGGCTGAGCCCTCAGGCAGGGTCCAGAGGG
  GGCCAGGGCCCCAAACATGGACAGCAGTGTCTCAAGATGCCAGGTCCCCGGGCCCCAGGTCTGCAGGGC
  GGTTCCAACAGAGATCCTGGCCAGCCCTGCGGTGGAGAGAGCACCCGCAGCAGCTCTGTCATCAACAAC
  TATCTGGATGCCAATGAGCCTGTGTCCTTGGAGGCCCGTCTTAGCCGCATGCACTTCCATGACAGTCAG
  AGGAAGGTCGACTATGTACTTGCCTACCACTACCGGAAACGCGGGGTGCACCTGGCCCAAGGCTTCCCT
  GGCCACTCGCTGGCTATCGTCTCCAATGGGGAGACAGGCAAGGAGCCTCATGCTGGGGGCCCAGGTGAC
  ATTGAGCTGGGACCGCTCGATGCCCTGGAGGAGGAGAGGAAGGAGCAGCGGGAGGAATTTGAGCACAAT
  CTGATGGAGGCTGGACTGGAGCTTGAGAAGGACTTGGAGAATAAAAGCCAGGGATCCATCTTTGTCCGG
  ATACACGCCCCGTGGCAGGTGCTGGCCAGAGAGGCAGAATTCTTGAAGATCAAAGTTCCTACCAAGAAA
  GAGATGTACGAGATCAAAGCAGGAGGCAGCATTGCAAAGAAGTTCAGCGCGGCTCTGCAGAAGCTGAGC
  TCGCACCTGCAGCCCCGAGTTCCAGAACACAGCAACAACAAGATGAAAAACCTCTCCTACCCATTCTCC
  AGGGAGAAGATGTACCTGTACAACATCCAGGAAAAGGACACCTTCTTTGATAATGCCACCCGCAGCCGC
  ATTGTGCACGAGATCCTGAAGCGCACAGCCTGCTCCCGAGCCAACAACACGATGGGTATTAACTCTCTG
  ATCGCAAACAATATCTATGAGGCTGCCTACCCTCTTCATGACGGTGAATACGATAGTCCAGAGGACGAT
  ATGAATGACAGGAAGCTGCTATATCAAGAATGGGCGCGCTATGGAGTGTTCTATAAGTTCCAACCTATT
  GACCTCATCAGAAAGTATTTTGGAGAAAAAATTGGACTGTATTTTGCCTGGCTGGGATTATATACATCA
  TTCCTCATCCCATCTTCTGTAATTGGAGTGATTGTGTTTCTTTATGGATGTGCAACAATTGAAGAAGAT
  ATTCCCAGCAGAGAGATGTGTGACCAGCAGAATGCCTTCACCATGTGTCCCCTGTGTGACAAGTCCTGT
  GATTACTGGAACCTCAGCTCAGCCTGTGGGACCGCGCAGGCCAGCCACCTGTTTGACAACCCTGCCACC
  GTCTTCTTCTCTATCTTCATGGCTCTGTGGGCTACCATGTTCCTGGAAAACTGGAAGAGGCTACAGATG
  CGACTGGGCTACTTTTGGGACCTGACTGGCATAGAAGAGGAAGAAGAACGTGCCCAGGAACATTCCAGG
  CCTGAGTATGAAACCAAAGTTCGAGAGAAAATGCTAAAGGAGAGCAACCAGTCTGCTGTCCAGAAATTG
  GAAACAAACACGACGGAGTGTGGCGATGAGGATGATGAAGATAAACTGACCTGGAAGGATCGTTTCCCA
  GGTTACCTGATGAACTTTGCCTCCATCTTATTCATGATTGCCCTGACATTCTCAATCGTCTTTGGGGTG
  ATAGTGTATCGAATAACAACTGCAGCCGCTCTGTCTCTCAATAAGGCTACACGCTCCAATGTCCGGGTG
  ACAGTGACAGCAACAGCAGTCATCATCAACCTCGTGGTCATCCTCATCCTGGACGAGATCTACGGCGCT
  GTGGCCAAGTGGCTCACCAAAATTGAGGTTCCGAAAACAGAACAGACTTTTGAAGAGCGCCTGATCCTC
  AAAGCTTTCTTGCTCAAGTTTGTCAATGCCTACTCCCCCATCTTCTATGTGGCCTTTTTCAAAGGGAGG
  TTTGTGGGCAGGCCTGGAAGCTACGTCTATGTATTCGATGGTTACCGCATGGAAGAGTGTGCTCCAGGG
  GGCTGTCTCATGGAGCTCTGCATTCAGCTCAGCATCATCATGTTGGGGAAGCAGTTGATCCAGAACAAC
  ATCTTTGAGATTGGAGTCCCGAAGCTAAAGAAACTATTTCGAAAGCTGAAAGATGAGACCGAAGCTGGA
  GAAACTGACTCTGCCCATTCGAAACATCCAGAGCAGTGGGACCTAGACTACAGCTTGGAACCATACACA
  GGACTGACTCCGGAGTACATGGAAATGATCATCCAGTTTGGTTTTGTCACCCTCTTCGTGGCCTCCTTT
  CCCCTGGCACCTGTGTTTGCCCTCCTCAACAACGTCATTGAAGTGCGGCTCGATGCAAAGAAGTTTGTT
  ACAGAGCTGAGACGGCCGGATGCTGTAAGAACCAAAGATATCGGAATCTGGTTTGACATTCTCTCTGGA
  ATTGGCAAGTTCTCTGTTATCAGCAACGCTTTTGTCATTGCGATCACCTCCGACTTTATCCCCCGCCTG
  GTGTACCAGTACTCCTACAGTCACAATGGGACTCTGCACGGCTTTGTCAACCACACCCTCTCCTTTTTC
  AACGTCAGCCAGCTGAAGGAGGGGACGCAGCCAGAAAACTCACAGTTTGACCAGGAGGTTCAGTTCTGC
  AGGTTTAAGGATTACCGAGAGCCGCCATGGGCCCCGAACCCTTATGAGTTTTCGAAACAGTACTGGTTT
  ATTCTGTCCGCCCGTCTGGCTTTTGTCATAATCTTCCAGAACCTCGTGATGTTCCTGAGCGTCCTCGTG
  GACTGGATGATTCCAGACATCCCCACGGACATCAGCGACCAGATCAAGAAAGAGAAGAGCTTATTAGTG
  GATTTCTTCCTGAAAGAGGAGCATGAGAAGCTCAAGCTGATGGATGAGCCGGCTCTGAGGAGCCCAGGA
  GGTGGGGATCGAAGCAGGAGCCGGGCAGCCAGCTCAGCACCTTCAGGCCAAAGCCAGCTGGGCAGCATG
  ATGTCGTCAGGCTCTCAGCACACCAATGTG
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG239867
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MPEDIPLLPGSPRRLSPQAGSRGGQGPKHGQQCLKMPGPRAPGLQGGSNRDPGQPCGGESTRSSSVINN
  YLDANEPVSLEARLSRMHFHDSQRKVDYVLAYHYRKRGVHLAQGFPGHSLAIVSNGETGKEPHAGGPGD
  IELGPLDALEEERKEQREEFEHNLMEAGLELEKDLENKSQGSIFVRIHAPWQVLAREAEFLKIKVPTKK
  EMYEIKAGGSIAKKFSAALQKLSSHLQPRVPEHSNNKMKNLSYPFSREKMYLYNIQEKDTFFDNATRSR
  IVHEILKRTACSRANNTMGINSLIANNIYEAAYPLHDGEYDSPEDDMNDRKLLYQEWARYGVFYKFQPI
  DLIRKYFGEKIGLYFAWLGLYTSFLIPSSVIGVIVFLYGCATIEEDIPSREMCDQQNAFTMCPLCDKSC
  DYWNLSSACGTAQASHLFDNPATVFFSIFMALWATMFLENWKRLQMRLGYFWDLTGIEEEEERAQEHSR
  PEYETKVREKMLKESNQSAVQKLETNTTECGDEDDEDKLTWKDRFPGYLMNFASILFMIALTFSIVFGV
  IVYRITTAAALSLNKATRSNVRVTVTATAVIINLVVILILDEIYGAVAKWLTKIEVPKTEQTFEERLIL
  KAFLLKFVNAYSPIFYVAFFKGRFVGRPGSYVYVFDGYRMEECAPGGCLMELCIQLSIIMLGKQLIQNN
  IFEIGVPKLKKLFRKLKDETEAGETDSAHSKHPEQWDLDYSLEPYTGLTPEYMEMIIQFGFVTLFVASF
  PLAPVFALLNNVIEVRLDAKKFVTELRRPDAVRTKDIGIWFDILSGIGKFSVISNAFVIAITSDFIPRL
  VYQYSYSHNGTLHGFVNHTLSFFNVSQLKEGTQPENSQFDQEVQFCRFKDYREPPWAPNPYEFSKQYWF
  ILSARLAFVIIFQNLVMFLSVLVDWMIPDIPTDISDQIKKEKSLLVDFFLKEEHEKLKLMDEPALRSPG
  GGDRSRSRAASSAPSGQSQLGSMMSSGSQHTNV
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001278597
• ORF Size: 2997 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.

Reference Data

• RefSeq: NM_001278597.3
• RefSeq Size: 3729 bp
• RefSeq ORF: 3000 bp
• Locus ID: 57101
• Cytogenetics: 12p13.31
• Protein Families: Transmembrane
• MW: 114.1 kDa
• Gene Summary: ANO2 belongs to a family of calcium-activated chloride channels (CaCCs) (reviewed by Hartzell et al., 2009 [PubMed 19015192]). [supplied by OMIM, Jan 2011]