TMEM16B (ANO2) (NM_001278596) Human Tagged ORF Clone

CAT#: RG239870

ANO2 (GFP-tagged) - Human anoctamin 2, calcium activated chloride channel (ANO2), transcript variant 1

Specifications

Product Data

• Tag: TurboGFP
• Symbol: ANO2
• Synonyms: C12orf3; TMEM16B
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG239870 representing NM_001278596.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGCGACTCCCGGGCCGCGCGATATACCCCTGCTCCCTGGCTCCCCACGCCGGCTGAGCCCTCAGGCA
  GGGTCCAGAGGGGGCCAGGGCCCCAAACATGGACAGCAGTGTCTCAAGATGCCAGGTCCCCGGGCCCCA
  GGTCTGCAGGGCGGTTCCAACAGAGATCCTGGCCAGCCCTGCGGTGGAGAGAGCACCCGCAGCAGCTCT
  GTCATCAACAACTATCTGGATGCCAATGAGCCTGTGTCCTTGGAGGCCCGTCTTAGCCGCATGCACTTC
  CATGACAGTCAGAGGAAGGTCGACTATGTACTTGCCTACCACTACCGGAAACGCGGGGTGCACCTGGCC
  CAAGGCTTCCCTGGCCACTCGCTGGCTATCGTCTCCAATGGGGAGACAGGCAAGGAGCCTCATGCTGGG
  GGCCCAGGTGACATTGAGCTGGGACCGCTCGATGCCCTGGAGGAGGAGAGGAAGGAGCAGCGGGAGGAA
  TTTGAGCACAATCTGATGGAGGCTGGACTGGAGCTTGAGAAGGACTTGGAGAATAAAAGCCAGGGATCC
  ATCTTTGTCCGGATACACGCCCCGTGGCAGGTGCTGGCCAGAGAGGCAGAATTCTTGAAGATCAAAGTT
  CCTACCAAGAAAGAGATGTACGAGATCAAAGCAGGAGGCAGCATTGCAAAGAAGTTCAGCGCGGCTCTG
  CAGAAGCTGAGCTCGCACCTGCAGCCCCGAGTTCCAGAACACAGCAACAACAAGATGAAAAACCTCTCC
  TACCCATTCTCCAGGGAGAAGATGTACCTGTACAACATCCAGGAAAAGGACACCTTCTTTGATAATGCC
  ACCCGCAGCCGCATTGTGCACGAGATCCTGAAGCGCACAGCCTGCTCCCGAGCCAACAACACGATGGGT
  ATTAACTCTCTGATCGCAAACAATATCTATGAGGCTGCCTACCCTCTTCATGACGGTGAATACGATAGT
  CCAGAGGACGATATGAATGACAGGAAGCTGCTATATCAAGAATGGGCGCGCTATGGAGTGTTCTATAAG
  TTCCAACCTATTGACCTCATCAGAAAGTATTTTGGAGAAAAAATTGGACTGTATTTTGCCTGGCTGGGA
  TTATATACATCATTCCTCATCCCATCTTCTGTAATTGGAGTGATTGTGTTTCTTTATGGATGTGCAACA
  ATTGAAGAAGATATTCCCAGCAGAGAGATGTGTGACCAGCAGAATGCCTTCACCATGTGTCCCCTGTGT
  GACAAGTCCTGTGATTACTGGAACCTCAGCTCAGCCTGTGGGACCGCGCAGGCCAGCCACCTGTTTGAC
  AACCCTGCCACCGTCTTCTTCTCTATCTTCATGGCTCTGTGGGCTACCATGTTCCTGGAAAACTGGAAG
  AGGCTACAGATGCGACTGGGCTACTTTTGGGACCTGACTGGCATAGAAGAGGAAGAAGAACGTGCCCAG
  GAACATTCCAGGCCTGAGTATGAAACCAAAGTTCGAGAGAAAATGCTAAAGGAGAGCAACCAGTCTGCT
  GTCCAGAAATTGGAAACAAACACGACGGAGTGTGGCGATGAGGATGATGAAGATAAACTGACCTGGAAG
  GATCGTTTCCCAGGTTACCTGATGAACTTTGCCTCCATCTTATTCATGATTGCCCTGACATTCTCAATC
  GTCTTTGGGGTGATAGTGTATCGAATAACAACTGCAGCCGCTCTGTCTCTCAATAAGGCTACACGCTCC
  AATGTCCGGGTGACAGTGACAGCAACAGCAGTCATCATCAACCTCGTGGTCATCCTCATCCTGGACGAG
  ATCTACGGCGCTGTGGCCAAGTGGCTCACCAAAATTGAGGTTCCGAAAACAGAACAGACTTTTGAAGAG
  CGCCTGATCCTCAAAGCTTTCTTGCTCAAGTTTGTCAATGCCTACTCCCCCATCTTCTATGTGGCCTTT
  TTCAAAGGGAGGTTTGTGGGCAGGCCTGGAAGCTACGTCTATGTATTCGATGGTTACCGCATGGAAGAG
  TGTGCTCCAGGGGGCTGTCTCATGGAGCTCTGCATTCAGCTCAGCATCATCATGTTGGGGAAGCAGTTG
  ATCCAGAACAACATCTTTGAGATTGGAGTCCCGAAGCTAAAGAAACTATTTCGAAAGCTGAAAGATGAG
  ACCGAAGCTGGAGAAACTGACTCTGCCCATTCGAAACATCCAGAGCAGTGGGACCTAGACTACAGCTTG
  GAACCATACACAGGACTGACTCCGGAGTACATGGAAATGATCATCCAGTTTGGTTTTGTCACCCTCTTC
  GTGGCCTCCTTTCCCCTGGCACCTGTGTTTGCCCTCCTCAACAACGTCATTGAAGTGCGGCTCGATGCA
  AAGAAGTTTGTTACAGAGCTGAGACGGCCGGATGCTGTAAGAACCAAAGATATCGGAATCTGGTTTGAC
  ATTCTCTCTGGAATTGGCAAGTTCTCTGTTATCAGCAACGCTTTTGTCATTGCGATCACCTCCGACTTT
  ATCCCCCGCCTGGTGTACCAGTACTCCTACAGTCACAATGGGACTCTGCACGGCTTTGTCAACCACACC
  CTCTCCTTTTTCAACGTCAGCCAGCTGAAGGAGGGGACGCAGCCAGAAAACTCACAGTTTGACCAGGAG
  GTTCAGTTCTGCAGGTTTAAGGATTACCGAGAGCCGCCATGGGCCCCGAACCCTTATGAGTTTTCGAAA
  CAGTACTGGTTTATTCTGTCCGCCCGTCTGGCTTTTGTCATAATCTTCCAGAACCTCGTGATGTTCCTG
  AGCGTCCTCGTGGACTGGATGATTCCAGACATCCCCACGGACATCAGCGACCAGATCAAGAAAGAGAAG
  AGCTTATTAGTGGATTTCTTCCTGAAAGAGGAGCATGAGAAGCTCAAGCTGATGGATGAGCCGGCTCTG
  AGGAGCCCAGGAGGTGGGGATCGAAGCAGGAGCCGGGCAGCCAGCTCAGCACCTTCAGGCCAAAGCCAG
  CTGGGCAGCATGATGTCGTCAGGCTCTCAGCACACCAATGTG
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG239870
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MATPGPRDIPLLPGSPRRLSPQAGSRGGQGPKHGQQCLKMPGPRAPGLQGGSNRDPGQPCGGESTRSSS
  VINNYLDANEPVSLEARLSRMHFHDSQRKVDYVLAYHYRKRGVHLAQGFPGHSLAIVSNGETGKEPHAG
  GPGDIELGPLDALEEERKEQREEFEHNLMEAGLELEKDLENKSQGSIFVRIHAPWQVLAREAEFLKIKV
  PTKKEMYEIKAGGSIAKKFSAALQKLSSHLQPRVPEHSNNKMKNLSYPFSREKMYLYNIQEKDTFFDNA
  TRSRIVHEILKRTACSRANNTMGINSLIANNIYEAAYPLHDGEYDSPEDDMNDRKLLYQEWARYGVFYK
  FQPIDLIRKYFGEKIGLYFAWLGLYTSFLIPSSVIGVIVFLYGCATIEEDIPSREMCDQQNAFTMCPLC
  DKSCDYWNLSSACGTAQASHLFDNPATVFFSIFMALWATMFLENWKRLQMRLGYFWDLTGIEEEEERAQ
  EHSRPEYETKVREKMLKESNQSAVQKLETNTTECGDEDDEDKLTWKDRFPGYLMNFASILFMIALTFSI
  VFGVIVYRITTAAALSLNKATRSNVRVTVTATAVIINLVVILILDEIYGAVAKWLTKIEVPKTEQTFEE
  RLILKAFLLKFVNAYSPIFYVAFFKGRFVGRPGSYVYVFDGYRMEECAPGGCLMELCIQLSIIMLGKQL
  IQNNIFEIGVPKLKKLFRKLKDETEAGETDSAHSKHPEQWDLDYSLEPYTGLTPEYMEMIIQFGFVTLF
  VASFPLAPVFALLNNVIEVRLDAKKFVTELRRPDAVRTKDIGIWFDILSGIGKFSVISNAFVIAITSDF
  IPRLVYQYSYSHNGTLHGFVNHTLSFFNVSQLKEGTQPENSQFDQEVQFCRFKDYREPPWAPNPYEFSK
  QYWFILSARLAFVIIFQNLVMFLSVLVDWMIPDIPTDISDQIKKEKSLLVDFFLKEEHEKLKLMDEPAL
  RSPGGGDRSRSRAASSAPSGQSQLGSMMSSGSQHTNV
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001278596
• ORF Size: 3009 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.

Reference Data

• RefSeq: NM_001278596.3
• RefSeq Size: 3711 bp
• RefSeq ORF: 3012 bp
• Locus ID: 57101
• Cytogenetics: 12p13.31
• Protein Families: Transmembrane
• MW: 114.4 kDa
• Gene Summary: ANO2 belongs to a family of calcium-activated chloride channels (CaCCs) (reviewed by Hartzell et al., 2009 [PubMed 19015192]). [supplied by OMIM, Jan 2011]