DDX46 (NM_001300860) Human Tagged ORF Clone

CAT#: RG239901

DDX46 (GFP-tagged) - Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 46 (DDX46), transcript variant 1

Specifications

Product Data

• Tag: TurboGFP
• Symbol: DDX46
• Synonyms: Prp5; PRPF5
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG239901 representing NM_001300860.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGGTCGGGAGTCACGCCACTATCGAAAACGATCGGCATCCCGGGGTCGCTCTGGAAGTCGGTCTAGA
  AGTCGCTCACCCTCAGACAAAAGAAGTAAACGTGGAGATGACAGACGGTCTAGAAGTAGAGATAGAGAT
  AGGAGGAGAGAGAGGTCTCGTAGCAGGGATAAAAGAAGATCTCGGTCAAGGGACAGGAAGCGTCTGAGA
  CGTTCCAGAAGTAGAGAGAGAGACAGAAGCCGAGAGCGAAGAAGATCTCGAAGTAGAGACAGGAGACGC
  TCAAGGAGTAGAAGCCGGGGCCGGCGATCCCGATCCTCCAGTCCTGGAAATAAAAGCAAGAAAACTGAG
  AATAGATCTAGGTCCAAAGAGAAAACTGATGGTGGGGAAAGTTCTAAAGAGAAGAAAAAAGACAAAGAT
  GACAAGGAGGATGAAAAAGAAAAAGATGCTGGCAACTTTGACCAGAATAAGCTGGAAGAAGAAATGAGA
  AAGCGAAAAGAAAGAGTAGAAAAATGGCGAGAAGAGCAACGTAAAAAGGCTATGGAAAACATAGGAGAA
  CTGAAAAAGGAAATCGAAGAGATGAAACAAGGGAAAAAGTGGAGTTTAGAGGACGATGATGATGACGAA
  GATGATCCTGCAGAAGCTGAAAAGGAGGGAAATGAAATGGAGGGTGAGGAGTTAGATCCATTAGATGCT
  TACATGGAAGAAGTGAAAGAGGAAGTAAAAAAATTTAACATGAGAAGTGTAAAAGGTGGTGGGGGAAAT
  GAAAAGAAGTCTGGGCCAACGGTCACAAAAGTTGTCACTGTTGTGACAACCAAAAAAGCAGTTGTGGAT
  TCTGATAAGAAGAAAGGTGAGCTGATGGAGAATGACCAGGATGCCATGGAGTATTCTTCAGAGGAGGAA
  GAAGTTGATCTTCAGACAGCCCTTACAGGGTATCAAACAAAACAGCGAAAGCTTCTAGAACCAGTTGAT
  CATGGAAAAATTGAGTATGAGCCATTTAGGAAAAACTTCTATGTTGAAGTTCCAGAACTAGCAAAAATG
  TCTCAAGAAGAGGTAAATGTGTTTCGATTGGAAATGGAGGGCATTACAGTTAAAGGAAAAGGTTGCCCC
  AAACCAATTAAATCCTGGGTCCAGTGTGGAATTTCCATGAAGATCTTAAATTCCCTCAAGAAGCATGGC
  TATGAAAAGCCCACGCCCATCCAAACCCAAGCTATTCCTGCTATAATGTCTGGACGAGATTTGATTGGC
  ATTGCCAAAACAGGAAGTGGAAAGACCATTGCTTTTCTGTTGCCCATGTTTAGACACATCATGGATCAG
  AGGTCATTAGAGGAAGGAGAGGGGCCAATAGCTGTCATCATGACTCCAACTCGAGAACTGGCTTTACAG
  ATTACTAAAGAGTGTAAGAAGTTTTCCAAGACTTTGGGACTTAGAGTGGTCTGTGTTTACGGAGGAACA
  GGAATCAGTGAGCAGATTGCTGAGCTGAAAAGAGGTGCTGAAATTATTGTTTGCACACCTGGTCGAATG
  ATTGACATGTTAGCCGCTAACAGTGGTCGGGTCACAAATCTTCGAAGAGTGACATATGTTGTTTTAGAT
  GAAGCAGACAGAATGTTTGACATGGGTTTTGAACCCCAGGTCATGCGCATCGTGGATAATGTTCGTCCT
  GATCGACAGACGGTTATGTTTTCAGCTACTTTCCCCAGAGCTATGGAGGCTTTGGCTCGCAGGATCCTC
  AGTAAACCTATTGAAGTACAAGTTGGAGGCAGGAGTGTGGTTTGCTCAGATGTGGAGCAACAAGTGATT
  GTGATTGAAGAAGAAAAGAAATTCTTGAAGTTACTTGAGCTTCTAGGCCATTATCAAGAGTCAGGATCT
  GTCATTATATTTGTGGATAAGCAGGAACATGCTGATGGTCTTCTTAAGGATTTAATGAGAGCATCTTAT
  CCTTGCATGTCTCTTCATGGAGGCATTGATCAATATGACAGAGATAGCATCATAAATGACTTTAAGAAT
  GGGACCTGCAAACTTCTTGTGGCTACCTCTGTTGCTGCCCGAGGTCTAGATGTGAAACATCTGATTCTT
  GTAGTAAATTATAGCTGCCCCAACCATTATGAGGATTATGTACACAGAGCAGGGCGGACTGGAAGAGCA
  GGAAACAAGGGTTATGCTTATACTTTTATCACAGAGGATCAAGCTCGCTATGCTGGTGACATAATTAAA
  GCTCTTGAATTGTCAGGGACTGCAGTACCTCCTGATTTAGAGAAACTGTGGAGTGATTTCAAAGATCAG
  CAGAAAGCTGAGGGGAAAATAATTAAAAAGAGTAGTGGGTTCTCTGGTAAGGGATTCAAGTTTGATGAA
  ACAGAACAAGCTTTGGCTAATGAGAGGAAGAAGTTACAAAAAGCAGCTCTTGGTCTACAAGATTCAGAT
  GATGAGGATGCTGCAGTTGATATTGATGAGCAAATTGAAAGCATGTTTAATTCAAAGAAGAGAGTAAAG
  GATATGGCTGCTCCTGGAACATCAAGTGTTCCTGCTCCAACTGCAGGAAATGCTGAGAAATTAGAAATT
  GCTAAGAGATTGGCTCTTAGAATCAATGCCCAGAAGAATTTGGGCATCGAGTCTCAGGTAGATGTGATG
  CAGCAGGCCACCAATGCAATTCTTAGGGGTGGCACCATTCTGGCTCCCACTGTTTCTGCAAAAACCATT
  GCAGAACAACTTGCTGAAAAGATCAATGCCAAGCTCAATTATGTGCCGTTAGAGAAACAAGAAGAAGAG
  AGACAGGATGGTGGACAGAATGAATCTTTTAAGAGATATGAAGAAGAATTAGAGATCAATGACTTCCCA
  CAGACTGCTAGGTGGAAAGTTACCTCTAAGGAAGCTCTGCAGAGAATCAGTGAATACTCTGAAGCCGCA
  ATTACAATCAGAGGAACCTACTTCCCTCCTGGCAAAGAACCCAAGGAAGGCGAGCGGAAGATTTACTTG
  GCAATTGAAAGTGCCAATGAACTGGCTGTGCAGAAAGCAAAGGCAGAAATCACCAGGCTCATAAAAGAA
  GAGCTGATCCGGCTGCAAAATTCATACCAACCAACAAATAAAGGAAGATACAAAGTCTTA
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG239901
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MGRESRHYRKRSASRGRSGSRSRSRSPSDKRSKRGDDRRSRSRDRDRRRERSRSRDKRRSRSRDRKRLR
  RSRSRERDRSRERRRSRSRDRRRSRSRSRGRRSRSSSPGNKSKKTENRSRSKEKTDGGESSKEKKKDKD
  DKEDEKEKDAGNFDQNKLEEEMRKRKERVEKWREEQRKKAMENIGELKKEIEEMKQGKKWSLEDDDDDE
  DDPAEAEKEGNEMEGEELDPLDAYMEEVKEEVKKFNMRSVKGGGGNEKKSGPTVTKVVTVVTTKKAVVD
  SDKKKGELMENDQDAMEYSSEEEEVDLQTALTGYQTKQRKLLEPVDHGKIEYEPFRKNFYVEVPELAKM
  SQEEVNVFRLEMEGITVKGKGCPKPIKSWVQCGISMKILNSLKKHGYEKPTPIQTQAIPAIMSGRDLIG
  IAKTGSGKTIAFLLPMFRHIMDQRSLEEGEGPIAVIMTPTRELALQITKECKKFSKTLGLRVVCVYGGT
  GISEQIAELKRGAEIIVCTPGRMIDMLAANSGRVTNLRRVTYVVLDEADRMFDMGFEPQVMRIVDNVRP
  DRQTVMFSATFPRAMEALARRILSKPIEVQVGGRSVVCSDVEQQVIVIEEEKKFLKLLELLGHYQESGS
  VIIFVDKQEHADGLLKDLMRASYPCMSLHGGIDQYDRDSIINDFKNGTCKLLVATSVAARGLDVKHLIL
  VVNYSCPNHYEDYVHRAGRTGRAGNKGYAYTFITEDQARYAGDIIKALELSGTAVPPDLEKLWSDFKDQ
  QKAEGKIIKKSSGFSGKGFKFDETEQALANERKKLQKAALGLQDSDDEDAAVDIDEQIESMFNSKKRVK
  DMAAPGTSSVPAPTAGNAEKLEIAKRLALRINAQKNLGIESQVDVMQQATNAILRGGTILAPTVSAKTI
  AEQLAEKINAKLNYVPLEKQEEERQDGGQNESFKRYEEELEINDFPQTARWKVTSKEALQRISEYSEAA
  ITIRGTYFPPGKEPKEGERKIYLAIESANELAVQKAKAEITRLIKEELIRLQNSYQPTNKGRYKVL
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001300860
• ORF Size: 3096 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.

Reference Data

• RefSeq: NM_001300860.2
• RefSeq Size: 5750 bp
• RefSeq ORF: 3099 bp
• Locus ID: 9879
• Cytogenetics: 5q31.1
• Protein Pathways: Spliceosome
• MW: 117.9 kDa
• Gene Summary: This gene encodes a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The protein encoded by this gene is a component of the 17S U2 snRNP complex; it plays an important role in pre-mRNA splicing. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2014]