CACNA2D2 (NM_001291101) Human Tagged ORF Clone

CAT#: RG239941

CACNA2D2 (GFP-tagged) - Human calcium channel, voltage-dependent, alpha 2/delta subunit 2 (CACNA2D2), transcript variant 4

Specifications

Product Data

• Tag: TurboGFP
• Symbol: CACNA2D2
• Synonyms: CACNA2D
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG239941 representing NM_001291101.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGCAGCACTGGGCCCGGCGTCTGGAGCAGGAGGTCGACGGCGTGATGCGGATTTTTGGAGGCGTCCAG
  CAGCTCCGTGAGATTTACAAGGACAACCGGAACCTGTTCGAGGTACAGGAGAATGAGCCTCAGAAGTTG
  GTGGAGAAGGTGGCAGGGGACATTGAGAGCCTTCTGGACAGGAAGGTGCAGGCCCTGAAGAGACTGGCT
  GATGCTGCAGAGAACTTCCAGAAAGCACACCGCTGGCAGGACAACATCAAGGAGGAAGACATCGTGTAC
  TATGACGCCAAGGCTGACGCTGAGCTGGACGACCCTGAGAGTGAGGATGTGGAAAGGGGGTCTAAGGCC
  AGCACCCTAAGGCTGGACTTCATCGAGGACCCAAACTTCAAGAACAAGGTCAACTATTCATACGCGGCT
  GTACAGATCCCTACGGACATCTACAAAGGCTCCACTGTCATCCTCAATGAGCTCAACTGGACAGAGGCC
  CTGGAGAATGTGTTCATGGAAAACCGCAGACAAGACCCCACACTGCTGTGGCAGGTCTTCGGCAGCGCC
  ACAGGAGTCACTCGCTACTACCCGGCCACCCCGTGGCGAGCCCCCAAGAAGATCGACCTGTACGATGTC
  CGAAGGAGACCCTGGTATATCCAGGGGGCCTCGTCACCCAAAGACATGGTCATCATCGTGGATGTGAGT
  GGCAGTGTGAGCGGCCTGACCCTGAAGCTGATGAAGACATCTGTCTGCGAGATGCTGGACACGCTGTCT
  GATGATGACTATGTGAATGTGGCCTCGTTCAACGAGAAGGCACAGCCTGTGTCATGCTTCACACACCTG
  GTGCAGGCCAATGTGCGCAACAAGAAGGTGTTCAAGGAAGCTGTGCAGGGCATGGTGGCCAAGGGCACC
  ACAGGCTACAAGGCCGGCTTTGAGTATGCCTTTGACCAGCTGCAGAACTCCAACATCACTCGGGCCAAC
  TGCAACAAGATGATCATGATGTTCACGGATGGTGGTGAGGACCGCGTGCAGGACGTCTTTGAGAAGTAC
  AATTGGCCAAACCGGACGGTGCGCGTGTTTACTTTCTCCGTGGGGCAGCATAACTATGACGTCACACCG
  CTGCAGTGGATGGCCTGTGCCAACAAAGGCTACTATTTTGAGATCCCTTCCATCGGAGCCATCCGCATC
  AACACACAGGAATATCTAGATGTGTTGGGCAGGCCCATGGTGCTGGCAGGCAAGGAGGCCAAGCAGGTG
  CAGTGGACCAACGTGTATGAGGATGCACTGGGACTGGGGTTGGTGGTAACAGGGACCCTCCCTGTTTTC
  AACCTGACACAGGATGGCCCTGGGGAAAAGAAGAACCAGCTGATCCTGGGCGTGATGGGCATTGACGTG
  GCTCTGAATGACATCAAGAGGCTGACCCCCAACTACACGCTTGGAGCCAACGGCTATGTGTTTGCCATT
  GACCTGAACGGCTACGTGTTGCTGCACCCCAATCTCAAGCCCCAGACCACCAACTTCCGGGAGCCTGTG
  ACTCTGGACTTCCTGGATGCGGAGCTAGAGGATGAGAACAAGGAAGAGATCCGTCGGAGCATGATTGAT
  GGCAACAAGGGCCACAAGCAGATCAGAACGTTGGTCAAGTCCCTGGATGAGAGGTACATAGATGAGGTG
  ACACGGAACTACACCTGGGTGCCTATAAGGAGCACTAACTACAGCCTGGGGCTGGTGCTCCCACCCTAC
  AGCACCTTCTACCTCCAAGCCAATCTCAGTGACCAGATCCTGCAGGTCAAGTATTTTGAGTTCCTGCTC
  CCCAGCAGCTTTGAGTCTGAAGGACACGTTTTCATTGCTCCCAGAGAGTACTGCAAGGACCTGAATGCC
  TCAGACAACAACACCGAGTTCCTGAAAAACTTTATTGAGCTCATGGAGAAAGTGACTCCAGACTCCAAG
  CAGTGCAACAACTTCCTTCTGCACAACCTGATCTTGGACACGGGCATCACGCAGCAGCTGGTAGAGCGT
  GTGTGGAGGGACCAGGATCTCAACACGTACAGCCTACTGGCCGTGTTCGCTGCCACAGACGGTGGCATC
  ACCCGAGTCTTCCCCAACAAGGCAGCTGAGGACTGGACAGAGAACCCTGAGCCCTTCAATGCCAGCTTC
  TACCGCCGCAGCCTGGATAACCACGGTTATGTCTTCAAGCCCCCACACCAGGATGCCCTGTTAAGGCCG
  CTGGAGCTGGAGAATGACACTGTGGGCATCCTCGTCAGCACAGCTGTGGAGCTCAGCCTAGGCAGGCGC
  ACACTGAGGCCAGCAGTGGTGGGCGTCAAGCTGGACCTAGAGGCTTGGGCTGAGAAGTTCAAGGTGCTA
  GCCAGCAACCGTACCCACCAAGACCAGCCTCAGAAGTGCGGCCCCAACAGCCACTGTGAGATGGACTGC
  GAGGTTAACAATGAGGACTTACTCTGTGTCCTCATTGATGATGGAGGATTCCTGGTGCTGTCAAACCAG
  AACCATCAGTGGGACCAGGTGGGCAGGTTCTTCAGTGAGGTGGATGCCAACCTGATGCTGGCACTCTAC
  AATAACTCCTTCTACACCCGCAAGGAGTCCTATGACTATCAGGCAGCCTGTGCCCCTCAGCCCCCTGGC
  AACCTGGGTGCTGCACCCCGGGGTGTCTTTGTGCCCACCGTTGCAGATTTCCTTAACCTGGCCTGGTGG
  ACCTCTGCTGCCGCCTGGTCCCTGTTCCAGCAGCTTCTCTACGGCCTCATCTACCACAGCTGGTTCCAA
  GCAGACCCCGCGGAGGCCGAGGGGAGCCCCGAGACGCGCGAGAGCAGCTGCGTCATGAAACAGACCCAG
  TACTACTTCGGCTCGGTAAACGCCTCCTACAACGCCATCATCGACTGCGGAAACTGCTCCAGGCTGTTC
  CACGCGCAGAGACTGACCAACACCAATCTTCTCTTTGTGGTGGCCGAGAAGCCGCTGTGCAGCCAGTGC
  GAGGCTGGCCGGCTGCTGCAGAAGGAGACGCACTGCCCAGCGGACGGCCCGGAGCAGTGTGAGCTAGTG
  CAGAGACCGCGATACCGGAGAGGCCCGCACATCTGCTTCGACTACAACGCGACAGAAGATACCTCAGAC
  TGTGGCCGCGGGGCCTCCTTCCCGCCGTCGCTGGGCGTCCTGGTCTCCCTGCAACTGCTGCTCCTCCTG
  GGCCTGCCGCCCCGGCCGCAGCCTCAAGTCCTCGTCCACGCCTCTCGCCGCCTC
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG239941
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MQHWARRLEQEVDGVMRIFGGVQQLREIYKDNRNLFEVQENEPQKLVEKVAGDIESLLDRKVQALKRLA
  DAAENFQKAHRWQDNIKEEDIVYYDAKADAELDDPESEDVERGSKASTLRLDFIEDPNFKNKVNYSYAA
  VQIPTDIYKGSTVILNELNWTEALENVFMENRRQDPTLLWQVFGSATGVTRYYPATPWRAPKKIDLYDV
  RRRPWYIQGASSPKDMVIIVDVSGSVSGLTLKLMKTSVCEMLDTLSDDDYVNVASFNEKAQPVSCFTHL
  VQANVRNKKVFKEAVQGMVAKGTTGYKAGFEYAFDQLQNSNITRANCNKMIMMFTDGGEDRVQDVFEKY
  NWPNRTVRVFTFSVGQHNYDVTPLQWMACANKGYYFEIPSIGAIRINTQEYLDVLGRPMVLAGKEAKQV
  QWTNVYEDALGLGLVVTGTLPVFNLTQDGPGEKKNQLILGVMGIDVALNDIKRLTPNYTLGANGYVFAI
  DLNGYVLLHPNLKPQTTNFREPVTLDFLDAELEDENKEEIRRSMIDGNKGHKQIRTLVKSLDERYIDEV
  TRNYTWVPIRSTNYSLGLVLPPYSTFYLQANLSDQILQVKYFEFLLPSSFESEGHVFIAPREYCKDLNA
  SDNNTEFLKNFIELMEKVTPDSKQCNNFLLHNLILDTGITQQLVERVWRDQDLNTYSLLAVFAATDGGI
  TRVFPNKAAEDWTENPEPFNASFYRRSLDNHGYVFKPPHQDALLRPLELENDTVGILVSTAVELSLGRR
  TLRPAVVGVKLDLEAWAEKFKVLASNRTHQDQPQKCGPNSHCEMDCEVNNEDLLCVLIDDGGFLVLSNQ
  NHQWDQVGRFFSEVDANLMLALYNNSFYTRKESYDYQAACAPQPPGNLGAAPRGVFVPTVADFLNLAWW
  TSAAAWSLFQQLLYGLIYHSWFQADPAEAEGSPETRESSCVMKQTQYYFGSVNASYNAIIDCGNCSRLF
  HAQRLTNTNLLFVVAEKPLCSQCEAGRLLQKETHCPADGPEQCELVQRPRYRRGPHICFDYNATEDTSD
  CGRGASFPPSLGVLVSLQLLLLLGLPPRPQPQVLVHASRRL
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001291101
• ORF Size: 3228 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.

Reference Data

• RefSeq: NM_001291101.1, NP_001278030.1
• RefSeq Size: 5461 bp
• RefSeq ORF: 3231 bp
• Locus ID: 9254
• Cytogenetics: 3p21.31
• Protein Families: Druggable Genome, Ion Channels: Other
• Protein Pathways: Arrhythmogenic right ventricular cardiomyopathy (ARVC), Cardiac muscle contraction, Dilated cardiomyopathy, Hypertrophic cardiomyopathy (HCM), MAPK signaling pathway
• MW: 122.6 kDa
• Gene Summary: Calcium channels mediate the entry of calcium ions into the cell upon membrane polarization. This gene encodes the alpha-2/delta subunit of the voltage-dependent calcium channel complex. The complex consists of the main channel-forming subunit alpha-1, and auxiliary subunits alpha-2/delta, beta, and gamma. The auxiliary subunits function in the assembly and membrane localization of the complex, and modulate calcium currents and channel activation/inactivation kinetics. The subunit encoded by this gene undergoes post-translational cleavage to yield the extracellular alpha2 peptide and a membrane-anchored delta polypeptide. This subunit is a receptor for the antiepileptic drug, gabapentin. Mutations in this gene are associated with early infantile epileptic encephalopathy. Single nucleotide polymorphisms in this gene are correlated with increased sensitivity to opioid drugs. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Mar 2014]