UPF1 (NM_001297549) Human Tagged ORF Clone

CAT#: RG239991

UPF1 (GFP-tagged) - Human UPF1 regulator of nonsense transcripts homolog (yeast) (UPF1), transcript variant 1

Specifications

Product Data

• Tag: TurboGFP
• Symbol: UPF1
• Synonyms: HUPF1; NORF1; pNORF1; RENT1; smg-2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG239991 representing NM_001297549.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGAGCGTGGAGGCGTACGGGCCCAGCTCGCAGACTCTCACTTTCCTGGACACGGAGGAGGCCGAGCTG
  CTTGGCGCCGACACACAGGGCTCCGAGTTCGAGTTCACCGACTTTACTCTTCCTAGCCAGACGCAGACG
  CCCCCCGGCGGCCCCGGCGGCCCGGGCGGTGGCGGCGCGGGAGGCCCGGGCGGCGCGGGCGCGGGCGCT
  GCGGCGGGACAGCTCGACGCGCAGGTTGGGCCCGAAGGCATCCTGCAGAACGGGGCTGTGGACGACAGT
  GTAGCCAAGACCAGCCAGTTGTTGGCTGAGTTGAACTTCGAGGAAGATGAAGAAGACACCTATTACACG
  AAGGACCTCCCCATACACGCCTGCAGTTACTGTGGAATACACGATCCTGCCTGCGTGGTTTACTGTAAT
  ACCAGCAAGAAGTGGTTCTGCAACGGACGTGGAAATACTTCTGGCAGCCACATTGTAAATCACCTTGTG
  AGGGCAAAATGCAAAGAGGTGACCCTGCACAAGGACGGGCCCCTGGGGGAGACAGTCCTGGAGTGCTAC
  AACTGCGGCTGTCGCAACGTCTTCCTCCTCGGCTTCATCCCGGCCAAAGCTGACTCAGTGGTGGTGCTG
  CTGTGCAGGCAGCCCTGTGCCAGCCAGAGCAGCCTCAAGGACATCAACTGGGACAGCTCGCAGTGGCAG
  CCGCTGATCCAGGACCGCTGCTTCCTGTCCTGGCTGGTCAAGATCCCCTCCGAGCAGGAGCAGCTGCGG
  GCACGCCAGATCACGGCACAGCAGATCAACAAGCTGGAGGAGCTGTGGAAGGAAAACCCTTCTGCCACG
  CTGGAGGACCTGGAGAAGCCGGGGGTGGACGAGGAGCCGCAGCATGTCCTCCTGCGGTACGAGGACGCC
  TACCAGTACCAGAACATATTCGGGCCCCTGGTCAAGCTGGAGGCCGACTACGACAAGAAGCTGAAGGAG
  TCCCAGACTCAAGATAACATCACTGTCAGGTGGGACCTGGGCCTTAACAAGAAGAGAATCGCCTACTTC
  ACTTTGCCCAAGACTGACTCTGGTAATGAGGATTTAGTCATAATTTGGTTAAGAGACATGCGGCTCATG
  CAGGGGGATGAGATATGCCTGCGGTACAAAGGGGACCTTGCGCCCCTGTGGAAAGGGATCGGCCACGTC
  ATCAAGGTCCCTGATAATTATGGCGATGAGATCGCCATTGAGCTGCGGAGCAGCGTGGGTGCACCTGTG
  GAGGTGACTCACAACTTCCAGGTGGATTTTGTGTGGAAGTCGACCTCCTTTGACAGGATGCAGAGCGCA
  TTGAAAACGTTTGCCGTGGATGAGACCTCGGTGTCTGGCTACATCTACCACAAGCTGTTGGGCCACGAG
  GTGGAGGACGTAATCATCAAGTGCCAGCTGCCCAAGCGCTTCACGGCGCAGGGCCTCCCCGACCTCAAC
  CACTCCCAGGTTTATGCCGTGAAGACTGTGCTGCAAAGACCACTGAGCCTGATCCAGGGCCCGCCAGGC
  ACGGGGAAGACGGTGACGTCGGCCACCATCGTCTACCACCTGGCCCGGCAAGGCAACGGGCCGGTGCTG
  GTGTGTGCTCCGAGCAACATCGCCGTGGACCAGCTAACGGAGAAGATCCACCAGACGGGGCTAAAGGTC
  GTGCGCCTCTGCGCCAAGAGCCGTGAGGCCATCGACTCCCCGGTGTCTTTTCTGGCCCTGCACAACCAG
  ATCAGGAACATGGACAGCATGCCTGAGCTGCAGAAGCTGCAGCAGCTGAAAGACGAGACTGGGGAGCTG
  TCGTCTGCCGACGAGAAGCGGTACCGGGCCTTGAAGCGCACCGCAGAGAGAGAGCTGCTGATGAACGCA
  GATGTCATCTGCTGCACATGTGTGGGCGCCGGTGACCCGAGGCTGGCCAAGATGCAGTTCCGCTCCATT
  TTAATCGACGAAAGCACCCAGGCCACCGAGCCGGAGTGCATGGTTCCCGTGGTCCTCGGGGCCAAGCAG
  CTGATCCTTGTAGGCGACCACTGCCAGCTGGGCCCAGTGGTGATGTGCAAGAAGGCGGCCAAGGCCGGG
  CTGTCACAGTCGCTCTTCGAGCGCCTGGTGGTGCTGGGCATCCGGCCCATCCGCCTGCAGGTCCAGTAC
  CGGATGCACCCTGCACTCAGCGCCTTCCCATCCAACATCTTCTACGAGGGCTCCCTCCAGAATGGTGTC
  ACTGCAGCGGATCGTGTGAAGAAGGGATTTGACTTCCAGTGGCCCCAACCCGATAAACCGATGTTCTTC
  TACGTGACCCAGGGCCAAGAGGAGATTGCCAGCTCGGGCACCTCCTACCTGAACAGGACCGAGGCTGCG
  AACGTGGAGAAGATCACCACGAAGTTGCTGAAGGCAGGCGCCAAGCCGGACCAGATTGGCATCATCACG
  CCCTACGAGGGCCAGCGCTCCTACCTGGTGCAGTACATGCAGTTCAGCGGCTCCCTGCACACCAAGCTC
  TACCAGGAGGTGGAGATCGCCAGTGTGGACGCCTTTCAGGGACGCGAGAAGGACTTCATCATCCTGTCC
  TGTGTGCGGGCCAACGAGCACCAAGGCATTGGCTTTTTAAATGACCCCAGGCGTCTGAACGTGGCCCTG
  ACCAGAGCAAGGTATGGCGTCATCATTGTGGGCAACCCGAAGGCACTATCAAAGCAGCCGCTCTGGAAC
  CACCTGCTGAACTACTATAAGGAGCAGAAGGTGCTGGTGGAGGGGCCGCTCAACAACCTGCGTGAGAGC
  CTCATGCAGTTCAGCAAGCCACGGAAGCTGGTCAACACTATCAACCCGGGAGCCCGCTTCATGACCACA
  GCCATGTATGATGCCCGGGAGGCCATCATCCCAGGCTCCGTCTATGATCGGAGCAGCCAGGGCCGGCCT
  TCCAGCATGTACTTCCAGACCCATGACCAGATTGGCATGATCAGTGCCGGCCCTAGCCACGTGGCTGCC
  ATGAACATTCCCATCCCCTTCAACCTGGTCATGCCACCCATGCCACCGCCTGGCTATTTTGGACAAGCC
  AACGGGCCTGCTGCAGGGCGAGGCACCCCGAAAGGCAAGACTGGTCGTGGGGGACGCCAGAAGAACCGC
  TTTGGGCTTCCTGGACCCAGCCAGACTAACCTCCCCAACAGCCAAGCCAGCCAGGATGTGGCGTCACAG
  CCCTTCTCTCAGGGCGCCCTGACGCAGGGCTACATCTCCATGAGCCAGCCTTCCCAGATGAGCCAGCCC
  GGCCTCTCCCAGCCGGAGCTGTCCCAGGACAGTTACCTTGGTGACGAGTTTAAATCACAAATCGACGTG
  GCGCTCTCACAGGACTCCACGTACCAGGGAGAGCGGGCTTACCAGCATGGCGGGGTGACGGGGCTGTCC
  CAGTAT
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG239991
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MSVEAYGPSSQTLTFLDTEEAELLGADTQGSEFEFTDFTLPSQTQTPPGGPGGPGGGGAGGPGGAGAGA
  AAGQLDAQVGPEGILQNGAVDDSVAKTSQLLAELNFEEDEEDTYYTKDLPIHACSYCGIHDPACVVYCN
  TSKKWFCNGRGNTSGSHIVNHLVRAKCKEVTLHKDGPLGETVLECYNCGCRNVFLLGFIPAKADSVVVL
  LCRQPCASQSSLKDINWDSSQWQPLIQDRCFLSWLVKIPSEQEQLRARQITAQQINKLEELWKENPSAT
  LEDLEKPGVDEEPQHVLLRYEDAYQYQNIFGPLVKLEADYDKKLKESQTQDNITVRWDLGLNKKRIAYF
  TLPKTDSGNEDLVIIWLRDMRLMQGDEICLRYKGDLAPLWKGIGHVIKVPDNYGDEIAIELRSSVGAPV
  EVTHNFQVDFVWKSTSFDRMQSALKTFAVDETSVSGYIYHKLLGHEVEDVIIKCQLPKRFTAQGLPDLN
  HSQVYAVKTVLQRPLSLIQGPPGTGKTVTSATIVYHLARQGNGPVLVCAPSNIAVDQLTEKIHQTGLKV
  VRLCAKSREAIDSPVSFLALHNQIRNMDSMPELQKLQQLKDETGELSSADEKRYRALKRTAERELLMNA
  DVICCTCVGAGDPRLAKMQFRSILIDESTQATEPECMVPVVLGAKQLILVGDHCQLGPVVMCKKAAKAG
  LSQSLFERLVVLGIRPIRLQVQYRMHPALSAFPSNIFYEGSLQNGVTAADRVKKGFDFQWPQPDKPMFF
  YVTQGQEEIASSGTSYLNRTEAANVEKITTKLLKAGAKPDQIGIITPYEGQRSYLVQYMQFSGSLHTKL
  YQEVEIASVDAFQGREKDFIILSCVRANEHQGIGFLNDPRRLNVALTRARYGVIIVGNPKALSKQPLWN
  HLLNYYKEQKVLVEGPLNNLRESLMQFSKPRKLVNTINPGARFMTTAMYDAREAIIPGSVYDRSSQGRP
  SSMYFQTHDQIGMISAGPSHVAAMNIPIPFNLVMPPMPPPGYFGQANGPAAGRGTPKGKTGRGGRQKNR
  FGLPGPSQTNLPNSQASQDVASQPFSQGALTQGYISMSQPSQMSQPGLSQPELSQDSYLGDEFKSQIDV
  ALSQDSTYQGERAYQHGGVTGLSQY
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001297549
• ORF Size: 3387 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.

Reference Data

• RefSeq: NM_001297549.2
• RefSeq Size: 5395 bp
• RefSeq ORF: 3390 bp
• Locus ID: 5976
• Cytogenetics: 19p13.11
• Protein Families: Druggable Genome
• MW: 124.8 kDa
• Gene Summary: 'This gene encodes a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein is located only in the cytoplasm. When translation ends, it interacts with the protein that is a functional homolog of yeast Upf2p to trigger mRNA decapping. Use of multiple polyadenylation sites has been noted for this gene. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]'