Phospholipase C beta 2 (PLCB2) (NM_001284298) Human Tagged ORF Clone

CAT#: RG240020

PLCB2 (GFP-tagged) - Human phospholipase C, beta 2 (PLCB2), transcript variant 3

Specifications

Product Data

• Tag: TurboGFP
• Symbol: PLCB2
• Synonyms: PLC-beta-2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG240020 representing NM_001284298.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGTCTCTGCTCAACCCTGTCCTGCTGCCCCCCAAGGTGAAGGCCTATCTGAGCCAAGGGGAGCGCTTC
  ATCAAATGGGATGATGAAACTACAGTTGCCTCTCCAGTTATCCTCCGTGTGGATCCTAAGGGCTACTAC
  TTATACTGGACGTATCAAAGTAAGGAGATGGAGTTTCTGGATATCACCAGCATCCGGGATACTCGCTTT
  GGGAAGTTTGCCAAGATGCCCAAGAGCCAGAAGCTCCGGGACGTCTTCAACATGGACTTTCCTGATAAC
  AGTTTCCTGCTGAAGACACTCACGGTGGTGTCCGGCCCGGACATGGTGGACCTCACCTTCCACAACTTC
  GTCTCCTACAAGGAGAACGTGGGCAAGGCCTGGGCTGAGGACGTACTGGCCCTAGTCAAACATCCGCTG
  ACGGCCAACGCCTCCCGCAGCACCTTCCTGGACAAGATCCTTGTGAAGCTCAAGATGCAGCTCAACTCT
  GAAGGGAAGATTCCGGTGAAGAACTTTTTCCAGATGTTTCCTGCTGACCGCAAGCGGGTGGAAGCTGCT
  CTCAGTGCCTGCCACCTCCCCAAAGGCAAAAATGACGCCATCAATCCTGAGGACTTCCCAGAACCTGTC
  TACAAGAGTTTCCTCATGAGCCTCTGTCCTCGGCCAGAAATAGATGAGATCTTCACTTCTTACCATGCT
  AAGGCCAAACCCTACATGACGAAGGAGCACCTGACCAAATTCATCAACCAGAAACAGCGGGACTCCCGG
  CTTAACTCCCTGCTGTTCCCGCCAGCACGGCCTGACCAGGTGCAGGGCCTCATCGACAAGTATGAGCCC
  AGTGGCATCAATGCACAGAGGGGCCAGCTGTCACCTGAAGGCATGGTCTGGTTTCTCTGTGGGCCAGAG
  AACAGCGTGCTGGCCCAGGACAAGCTGCTGCTCCACCACGACATGACGCAGCCACTCAATCATTACTTC
  ATCAACTCGTCCCACAACACCTACCTGACAGCCGGCCAGTTCTCAGGCCTCTCCTCGGCTGAGATGTAC
  CGCCAGGTGCTGCTCTCTGGCTGCCGTTGCGTGGAGCTAGACTGCTGGAAGGGGAAACCCCCTGACGAG
  GAGCCCATTATCACCCATGGCTTCACCATGACCACAGACATCTTCTTCAAAGAAGCAATTGAGGCTATT
  GCAGAAAGCGCCTTTAAGACCTCCCCCTATCCCATCATCCTGTCGTTTGAGAACCATGTGGACTCACCC
  CGCCAGCAGGCTAAGATGGCTGAGTATTGCCGGACGATCTTTGGGGATATGCTGCTCACAGAGCCCCTG
  GAAAAGTTCCCACTAAAACCAGGTGTCCCCCTGCCCAGCCCTGAGGATCTCAGGGGCAAGATCCTCATC
  AAGAACAAGAAGAACCAGTTTTCTGGCCCCACCTCCTCCAGTAAGGATACTGGTGGGGAGGCTGAGGGC
  AGCAGCCCACCCAGTGCCCCTGCAGGTGAGGGCACAGTGTGGGCTGGCGAGGAAGGGACTGAGCTGGAG
  GAGGAGGAGGTGGAAGAGGAAGAGGAGGAGGAGTCAGGAAACCTGGATGAAGAAGAGATTAAGAAGATG
  CAGTCGGATGAGGGCACAGCGGGCCTGGAAGTGACGGCTTATGAGGAGATGTCCAGCCTAGTCAATTAC
  ATCCAGCCCACCAAGTTCGTCTCCTTTGAGTTCTCTGCCCAAAAGAACCGAAGTTATGTCATCTCGTCC
  TTCACAGAGCTCAAGGCATATGACCTGCTCTCCAAGGCCTCGGTGCAGTTTGTGGACTACAACAAGCGC
  CAGATGAGCCGCATTTACCCCAAGGGAACCCGCATGGACTCCTCCAACTACATGCCCCAGATGTTCTGG
  AATGCTGGATGCCAGATGGTTGCCCTCAACTTCCAGACGATGGACTTGCCCATGCAGCAGAACATGGCA
  GTATTTGAGTTCAACGGGCAGAGCGGCTACCTCCTCAAGCATGAGTTCATGCGCCGGCCGGACAAGCAG
  TTCAACCCCTTCTCAGTGGACCGCATCGACGTGGTGGTGGCCACCACCCTTTCCATTACGGTGATCTCT
  GGGCAGTTCCTGTCAGAACGCAGCGTGCGCACCTATGTAGAAGTGGAGCTGTTTGGCCTTCCTGGGGAC
  CCCAAGAGGCGCTATCGAACTAAGCTGTCACCCAGTACTAACTCCATCAATCCTGTCTGGAAGGAGGAG
  CCCTTTGTCTTTGAGAAGATCTTGATGCCTGAGCTGGCCTCCCTCAGAGTGGCTGTGATGGAGGAAGGC
  AACAAGTTTCTTGGACACCGCATCATCCCCATCAATGCCCTAAATTCTGGGTACCACCACCTGTGCCTG
  CACAGTGAGAGCAACATGCCCCTCACCATGCCTGCGCTCTTCATCTTCCTGGAGATGAAGGACTACATA
  CCTGGTGCTTGGGCAGATCTCACTGTGGCCCTCGCCAACCCCATTAAGTTCTTCAGTGCCCATGACACG
  AAGTCTGTGAAGCTCAAGGAGGCCATGGGAGGTCTGCCTGAGAAGCCCTTCCCACTGGCGAGTCCAGTT
  GCCAGCCAGGTCAATGGGGCGTTGGCCCCAACGAGCAATGGGTCACCAGAGCCGCGGACCGCCAGCCTG
  GAGGAGCTCCGGGAGCTAAAGGGCGTGGTGAAGCTGCAGCGGCGGCACGAGAAGGAGCTGCGAGAGTTG
  GAGCGGCGCGGAGCGCGGCGCTGGGAGGAGCTGCTGCAGCGGGGCGCGGCGCAGCTGGCGGAGCTCGGG
  CCACCGGGCGTGGGGGGCGTCGGGGCCTGCAAGCTCGGTCCCGGCAAGGGCTCTCGCAAGAAGAGGAGC
  CTGCCCCGCGAGGAGAGCGCCGGAGCCGCGCCGGGCGAGGGCCCTGAGGGCGTGGACGGGCGCGTGCGG
  GAGCTGAAAGACAGGCTGGAGCTGGAGCTGCTGCGGCAGGGCGAGGAGCAGTACGAGTGCGTTCTGAAG
  CGCAAGGAGCAGCACGTGGCCGAGCAAATCTCCAAAATGATGGAGCTGGCCAGAGAGAAACAGGCGGCA
  GAGCTGAAGGCCCTGAAGGAGACGTCGGAGAACGACACCAAAGAGATGAAGAAAAAGCTGGAGACAAAG
  AGACTGGAGCGGATCCAGGGCATGACCAAAGTCACCACAGACAAGATGGCCCAGGAGAGGTTGAAGAGA
  GAGATTAACAACTCCCACATCCAGGAAGTAGTGCAGGTGATCAAGCAGATGACGGAGAACTTGGAGAGG
  CACCAGGAGAAGCTGGAGGAGAAGCAGGCGGCTTGCCTGGAACAGATACGGGAGATGGAAAAGCAGTTC
  CAGAAGGAGGCGCTGGCAGAGTACGAGGCCAGGATGAAGGGTCTGGAGGCAGAGGTGAAGGAGTCGGTG
  AGGGCCTGCCTCAGGACCTGCTTTCCCTCCGAGGCCAAGGACAAGCCTGAGAGGGCCTGCGAGTGCCCC
  CCAGAGCTGTGTGAGCAGGACCCACTCATAGCAAAGGCAGATGCCCAGGAGAGCCGCCTC
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG240020
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MSLLNPVLLPPKVKAYLSQGERFIKWDDETTVASPVILRVDPKGYYLYWTYQSKEMEFLDITSIRDTRF
  GKFAKMPKSQKLRDVFNMDFPDNSFLLKTLTVVSGPDMVDLTFHNFVSYKENVGKAWAEDVLALVKHPL
  TANASRSTFLDKILVKLKMQLNSEGKIPVKNFFQMFPADRKRVEAALSACHLPKGKNDAINPEDFPEPV
  YKSFLMSLCPRPEIDEIFTSYHAKAKPYMTKEHLTKFINQKQRDSRLNSLLFPPARPDQVQGLIDKYEP
  SGINAQRGQLSPEGMVWFLCGPENSVLAQDKLLLHHDMTQPLNHYFINSSHNTYLTAGQFSGLSSAEMY
  RQVLLSGCRCVELDCWKGKPPDEEPIITHGFTMTTDIFFKEAIEAIAESAFKTSPYPIILSFENHVDSP
  RQQAKMAEYCRTIFGDMLLTEPLEKFPLKPGVPLPSPEDLRGKILIKNKKNQFSGPTSSSKDTGGEAEG
  SSPPSAPAGEGTVWAGEEGTELEEEEVEEEEEEESGNLDEEEIKKMQSDEGTAGLEVTAYEEMSSLVNY
  IQPTKFVSFEFSAQKNRSYVISSFTELKAYDLLSKASVQFVDYNKRQMSRIYPKGTRMDSSNYMPQMFW
  NAGCQMVALNFQTMDLPMQQNMAVFEFNGQSGYLLKHEFMRRPDKQFNPFSVDRIDVVVATTLSITVIS
  GQFLSERSVRTYVEVELFGLPGDPKRRYRTKLSPSTNSINPVWKEEPFVFEKILMPELASLRVAVMEEG
  NKFLGHRIIPINALNSGYHHLCLHSESNMPLTMPALFIFLEMKDYIPGAWADLTVALANPIKFFSAHDT
  KSVKLKEAMGGLPEKPFPLASPVASQVNGALAPTSNGSPEPRTASLEELRELKGVVKLQRRHEKELREL
  ERRGARRWEELLQRGAAQLAELGPPGVGGVGACKLGPGKGSRKKRSLPREESAGAAPGEGPEGVDGRVR
  ELKDRLELELLRQGEEQYECVLKRKEQHVAEQISKMMELAREKQAAELKALKETSENDTKEMKKKLETK
  RLERIQGMTKVTTDKMAQERLKREINNSHIQEVVQVIKQMTENLERHQEKLEEKQAACLEQIREMEKQF
  QKEALAEYEARMKGLEAEVKESVRACLRTCFPSEAKDKPERACECPPELCEQDPLIAKADAQESRL
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001284298
• ORF Size: 3510 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.

Reference Data

• RefSeq: NM_001284298.2
• RefSeq Size: 4649 bp
• RefSeq ORF: 3513 bp
• Locus ID: 5330
• Cytogenetics: 15q15.1
• Protein Families: Druggable Genome
• Protein Pathways: Alzheimer's disease, Calcium signaling pathway, Chemokine signaling pathway, Gap junction, GnRH signaling pathway, Huntington's disease, Inositol phosphate metabolism, Long-term depression, Long-term potentiation, Melanogenesis, Metabolic pathways, Phosphatidylinositol signaling system, Taste transduction, Vascular smooth muscle contraction, Wnt signaling pathway
• MW: 133 kDa
• Gene Summary: 'The protein encoded by this gene is a phosphodiesterase that catalyzes the hydrolysis of phosphatidylinositol 4,5-bisphosphate to the second messengers inositol 1,4,5-trisphosphate (IP3) and diacylglycerol. The encoded protein is activated by G proteins and has been shown to be involved in the type 2 taste receptor signal transduction pathway. In addition, nuclear factor kappa B can regulate the transcription of this gene, whose protein product is also an important regulator of platelet responses. [provided by RefSeq, Jan 2017]'