DAP Kinase 1 (DAPK1) (NM_001288729) Human Tagged ORF Clone

CAT#: RG240139

DAPK1 (GFP-tagged) - Human death-associated protein kinase 1 (DAPK1), transcript variant 2

Specifications

Product Data

• Tag: TurboGFP
• Symbol: DAPK1
• Synonyms: DAPK; ROCO3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG240139 representing NM_001288729.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGACCGTGTTCAGGCAGGAAAACGTGGATGATTACTACGACACCGGCGAGGAACTTGGCAGTGGACAG
  TTTGCGGTTGTGAAGAAATGCCGTGAGAAAAGCACCGGCCTCCAGTATGCCGCCAAATTCATCAAGAAA
  AGGAGGACTAAGTCCAGCCGGCGGGGTGTGAGCCGCGAGGACATCGAGCGGGAGGTCAGCATCCTGAAG
  GAGATCCAGCACCCCAATGTCATCACCCTGCACGAGGTCTATGAGAACAAGACGGACGTCATCCTGATC
  TTGGAACTCGTTGCAGGTGGCGAGCTGTTTGACTTCTTAGCTGAAAAGGAATCTTTAACTGAAGAGGAA
  GCAACTGAATTTCTCAAACAAATTCTTAATGGTGTTTACTACCTGCACTCCCTTCAAATCGCCCACTTT
  GATCTTAAGCCTGAGAACATAATGCTTTTGGATAGAAATGTCCCCAAACCTCGGATCAAGATCATTGAC
  TTTGGGTTGGCCCATAAAATTGACTTTGGAAATGAATTTAAAAACATATTTGGGACTCCAGAGTTTGTC
  GCTCCTGAGATAGTCAACTATGAACCTCTTGGTCTTGAGGCAGATATGTGGAGTATCGGGGTAATAACC
  TATATCCTCCTAAGTGGGGCCTCCCCATTTCTTGGAGACACTAAGCAAGAAACGTTAGCAAATGTATCC
  GCTGTCAACTACGAATTTGAGGATGAATACTTCAGTAATACCAGTGCCCTAGCCAAAGATTTCATAAGA
  AGACTTCTGGTCAAGGATCCAAAGAAGAGAATGACAATTCAAGATAGTTTGCAGCATCCCTGGATCAAG
  CCTAAAGATACACAACAGGCACTTAGTAGAAAAGCATCAGCAGTAAACATGGAGAAATTCAAGAAGTTT
  GCAGCCCGGAAAAAATGGAAACAATCCGTTCGCTTGATATCACTGTGCCAAAGATTATCCAGGTCATTC
  CTGTCCAGAAGTAACATGAGTGTTGCCAGAAGCGATGATACTCTGGATGAGGAAGACTCCTTTGTGATG
  AAAGCCATCATCCATGCCATCAACGATGACAATGTCCCAGGCCTGCAGCACCTTCTGGGCTCATTATCC
  AACTATGATGTTAACCAACCCAACAAGCACGGGACACCTCCATTACTCATTGCTGCTGGCTGTGGGAAT
  ATTCAAATACTACAGTTGCTCATTAAAAGAGGCTCGAGAATCGATGTCCAGGATAAGGGCGGGTCCAAT
  GCCGTCTACTGGGCTGCTCGGCATGGCCACGTCGATACCTTGAAATTTCTCAGTGAGAACAAATGCCCT
  TTGGATGTGAAAGACAAGTCTGGAGAGATGGCCCTCCACGTGGCAGCTCGCTATGGCCATGCTGACGTG
  GCTCAGTTACTGTGCAGCTTCGGCTCAAATCCCAATATCCAGGACAAGGAAGAAGAAACCCCCCTGCAC
  TGTGCTGCTTGGCACGGCTATTACTCTGTGGCCAAAGCCCTTTGTGAAGCCGGCTGTAACGTGAACATC
  AAGAACCGAGAAGGAGAGACGCCCCTCCTGACAGCCTCTGCCAGGGGCTACCACGACATCGTGGAGTGT
  CTGGCCGAACATGGAGCCGACCTTAATGCTTGCGACAAGGACGGACACATTGCCCTTCATCTGGCTGTA
  AGACGGTGTCAGATGGAGGTAATCAAGACTCTCCTCAGCCAAGGGTGTTTCGTCGATTATCAAGACAGG
  CACGGCAATACTCCCCTCCATGTGGCATGTAAAGATGGCAACATGCCTATCGTGGTGGCCCTCTGTGAA
  GCAAACTGCAATTTGGACATCTCCAACAAGTATGGGCGAACGCCTCTGCACCTTGCGGCCAACAACGGA
  ATCCTAGACGTGGTCCGGTATCTCTGTCTGATGGGAGCCAGCGTTGAGGCGCTGACCACGGACGGAAAG
  ACGGCAGAAGATCTTGCTAGATCGGAACAGCACGAGCACGTAGCAGGTCTCCTTGCAAGACTTCGAAAG
  GATACGCACCGAGGACTCTTCATCCAGCAGCTCCGACCCACACAGAACCTGCAGCCAAGAATTAAGCTC
  AAGCTGTTTGGCCACTCGGGATCCGGGAAAACCACCCTTGTAGAATCTCTCAAGTGTGGGCTGCTGAGG
  AGCTTTTTCAGAAGGCGTCGGCCCAGACTGTCTTCCACCAACTCCAGCAGGTTCCCACCTTCACCCCTG
  GCTTCTAAGCCCACAGTCTCAGTGAGCATCAACAACCTGTACCCAGGCTGCGAGAACGTGAGTGTGAGG
  AGCCGCAGCATGATGTTCGAGCCGGGTCTTACCAAAGGGATGCTGGAGGTGTTTGTGGCCCCGACCCAC
  CACCCGCACTGCTCGGCCGATGACCAGTCCACCAAGGCCATCGACATCCAGAACGCTTATTTGAATGGA
  GTTGGCGATTTCAGCGTGTGGGAGTTCTCTGGAAATCCTGTGTATTTCTGCTGTTATGACTATTTTGCT
  GCAAATGATCCCACGTCAATCCATGTTGTTGTCTTTAGTCTAGAAGAGCCCTATGAGATCCAGCTGAAC
  CAAGTGATTTTCTGGCTCAGTTTCCTGAAGTCCCTTGTCCCAGTTGAAGAACCCATAGCCTTCGGTGGC
  AAGCTGAAGAACCCACTCCAAGTTGTCCTGGTGGCCACCCACGCTGACATCATGAATGTTCCTCGACCG
  GCTGGAGGCGAGTTTGGATATGACAAAGACACATCGTTGCTGAAAGAGATTAGGAACAGGTTTGGAAAT
  GATCTTCACATTTCAAATAAGCTGTTTGTTCTGGATGCTGGGGCTTCTGGGTCAAAGGACATGAAGGTA
  CTTCGAAATCATCTGCAAGAAATACGAAGCCAGATTGTTTCGGTCTGTCCTCCCATGACTCACCTGTGT
  GAGAAAATCATCTCCACGCTGCCTTCCTGGAGGAAGCTCAATGGACCCAACCAGCTGATGTCGCTGCAG
  CAGTTTGTGTACGACGTGCAGGACCAGCTGAACCCCCTGGCCAGCGAGGAGGACCTCAGGCGCATTGCT
  CAGCAGCTCCACAGCACAGGCGAGATCAACATCATGCAAAGTGAAACAGTTCAGGACGTGCTGCTCCTG
  GACCCCCGCTGGCTCTGCACAAACGTCCTGGGGAAGTTGCTGTCCGTGGAGACCCCACGGGCGCTGCAC
  CACTACCGGGGCCGCTACACCGTGGAGGACATCCAGCGCCTGGTGCCCGACAGCGACGTGGAGGAGCTG
  CTGCAGATCCTCGATGCCATGGACATCTGCGCCCGGGACCTGAGCAGCGGGACCATGGTGGACGTCCCA
  GCCCTGATCAAGACAGACAACCTGCACCGCTCCTGGGCTGATGAGGAGGACGAGGTGATGGTGTATGGT
  GGCGTGCGCATCGTGCCCGTGGAACACCTCACCCCCTTCCCATGTGGCATCTTTCACAAGGTCCAGGTG
  AACCTGTGCCGGTGGATCCACCAGCAAAGCACAGAGGGCGACGCGGACATCCGCCTGTGGGTGAATGGC
  TGCAAGCTGGCCAACCGTGGGGCCGAGCTGCTGGTGCTGCTGGTCAACCACGGCCAGGGCATTGAGGTC
  CAGGTCCGCGGCCTGGAGACGGAGAAGATCAAGTGCTGCCTGCTGCTGGACTCGGTGTGCAGCACCATT
  GAGAACGTCATGGCCACCACGCTGCCAGGGCTCCTGACCGTGAAGCATTACCTGAGCCCCCAGCAGCTG
  CGGGAGCACCATGAGCCCGTCATGATCTACCAGCCACGGGACTTCTTCCGGGCACAGACTCTGAAGGAA
  ACCTCACTGACCAACACCATGGGGGGGTACAAGGAAAGCTTCAGCAGCATCATGTGCTTCGGGTGTCAC
  GACGTCTACTCACAGGCCAGCCTCGGCATGGACATCCATGCATCAGACCTGAACCTCCTCACTCGGAGG
  AAACTGAGTCGCCTGCTGGACCCGCCCGACCCCCTGGGGAAGGACTGGTGCCTTCTCGCCATGAACTTA
  GGCCTCCCTGACCTCGTGGCAAAGTACAACACCAGTAACGGGGCTCCCAAGGATTTCCTCCCCAGCCCC
  CTCCACGCCCTGCTGCGGGAATGGACCACCTACCCTGAGAGCACAGTGGGCACCCTCATGTCCAAACTG
  AGGGAGCTGGGTCGCCGGGATGCCGCAGACTTTTTGCTGAAGGCATCCTCTGTGTTCAAAATCAACCTG
  GATGGCAATGGCCAGGAGGCCTATGCCTCGAGCTGCAACAGCGGCACCTCTTACAATTCCATTAGCTCT
  GTTGTATCCCGG
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG240139
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MTVFRQENVDDYYDTGEELGSGQFAVVKKCREKSTGLQYAAKFIKKRRTKSSRRGVSREDIEREVSILK
  EIQHPNVITLHEVYENKTDVILILELVAGGELFDFLAEKESLTEEEATEFLKQILNGVYYLHSLQIAHF
  DLKPENIMLLDRNVPKPRIKIIDFGLAHKIDFGNEFKNIFGTPEFVAPEIVNYEPLGLEADMWSIGVIT
  YILLSGASPFLGDTKQETLANVSAVNYEFEDEYFSNTSALAKDFIRRLLVKDPKKRMTIQDSLQHPWIK
  PKDTQQALSRKASAVNMEKFKKFAARKKWKQSVRLISLCQRLSRSFLSRSNMSVARSDDTLDEEDSFVM
  KAIIHAINDDNVPGLQHLLGSLSNYDVNQPNKHGTPPLLIAAGCGNIQILQLLIKRGSRIDVQDKGGSN
  AVYWAARHGHVDTLKFLSENKCPLDVKDKSGEMALHVAARYGHADVAQLLCSFGSNPNIQDKEEETPLH
  CAAWHGYYSVAKALCEAGCNVNIKNREGETPLLTASARGYHDIVECLAEHGADLNACDKDGHIALHLAV
  RRCQMEVIKTLLSQGCFVDYQDRHGNTPLHVACKDGNMPIVVALCEANCNLDISNKYGRTPLHLAANNG
  ILDVVRYLCLMGASVEALTTDGKTAEDLARSEQHEHVAGLLARLRKDTHRGLFIQQLRPTQNLQPRIKL
  KLFGHSGSGKTTLVESLKCGLLRSFFRRRRPRLSSTNSSRFPPSPLASKPTVSVSINNLYPGCENVSVR
  SRSMMFEPGLTKGMLEVFVAPTHHPHCSADDQSTKAIDIQNAYLNGVGDFSVWEFSGNPVYFCCYDYFA
  ANDPTSIHVVVFSLEEPYEIQLNQVIFWLSFLKSLVPVEEPIAFGGKLKNPLQVVLVATHADIMNVPRP
  AGGEFGYDKDTSLLKEIRNRFGNDLHISNKLFVLDAGASGSKDMKVLRNHLQEIRSQIVSVCPPMTHLC
  EKIISTLPSWRKLNGPNQLMSLQQFVYDVQDQLNPLASEEDLRRIAQQLHSTGEINIMQSETVQDVLLL
  DPRWLCTNVLGKLLSVETPRALHHYRGRYTVEDIQRLVPDSDVEELLQILDAMDICARDLSSGTMVDVP
  ALIKTDNLHRSWADEEDEVMVYGGVRIVPVEHLTPFPCGIFHKVQVNLCRWIHQQSTEGDADIRLWVNG
  CKLANRGAELLVLLVNHGQGIEVQVRGLETEKIKCCLLLDSVCSTIENVMATTLPGLLTVKHYLSPQQL
  REHHEPVMIYQPRDFFRAQTLKETSLTNTMGGYKESFSSIMCFGCHDVYSQASLGMDIHASDLNLLTRR
  KLSRLLDPPDPLGKDWCLLAMNLGLPDLVAKYNTSNGAPKDFLPSPLHALLREWTTYPESTVGTLMSKL
  RELGRRDAADFLLKASSVFKINLDGNGQEAYASSCNSGTSYNSISSVVSR
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001288729
• ORF Size: 4290 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.

Reference Data

• RefSeq: NM_001288729.1, NP_001275658.1
• RefSeq Size: 5787 bp
• RefSeq ORF: 4293 bp
• Locus ID: 1612
• Cytogenetics: 9q21.33
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Bladder cancer, Pathways in cancer
• MW: 160.5 kDa
• Gene Summary: 'Death-associated protein kinase 1 is a positive mediator of gamma-interferon induced programmed cell death. DAPK1 encodes a structurally unique 160-kD calmodulin dependent serine-threonine kinase that carries 8 ankyrin repeats and 2 putative P-loop consensus sites. It is a tumor suppressor candidate. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2013]'