NUP153 (NM_001278209) Human Tagged ORF Clone

CAT#: RG240167

NUP153 (GFP-tagged) - Human nucleoporin 153kDa (NUP153), transcript variant 1

Specifications

Product Data

• Tag: TurboGFP
• Symbol: NUP153
• Synonyms: HNUP153; N153
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG240167 representing NM_001278209.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGCCTCGGGAGCCGGAGGAGTCGGAGGGGGCGGTGGCGGCAAGATCCGGACGCGGCGTTGCCACCAG
  GGGCCAATTAAGCCTTACCAGCAGGGGCGACAACAGCATCAGGGCATTCTTAGCAGGGTTACAGAATCT
  GTTAAGAATATTGTGCCAGGGTGGCTACAAAGATACTTCAACAAGAATGAAGATGTATGCAGCTGTTCA
  ACAGACACAAGCGAGGTTCCACGCTGGCCAGAAAATAAAGAGGACCATCTGGTATATGCCGATGAGGAG
  AGCTCTAATATTACTGATGGGAGAATCACACCTGAGCCAGCAGTCAGTAATACAGAAGAACCTTCAACA
  ACTAGTACTGCTTCAAATTATCCAGATGTGTTAACAAGGCCTTCTCTTCATCGGAGCCATCTGAATTTT
  TCCATGTTGGAATCCCCTGCATTACACTGTCAGCCATCTACATCCTCGGCATTCCCAATTGGCAGTTCG
  GGATTTTCCCTTGTAAAGGAAATTAAAGATTCTACCTCTCAGCATGATGATGATAACATCTCAACTACC
  AGTGGTTTTTCTTCAAGAGCTTCTGATAAAGATATAACTGTTTCAAAGAACACTTCATTGCCACCTCTG
  TGGTCCCCAGAAGCTGAACGTTCTCACTCACTCTCACAGCACACTGCCACCAGCTCAAAAAAACCAGCA
  TTCAACTTGTCTGCCTTTGGAACACTTTCCCCTTCACTTGGGAATTCTTCAATCCTTAAAACCAGTCAG
  CTTGGAGATTCTCCTTTTTATCCTGGAAAAACAACATACGGTGGGGCAGCAGCTGCTGTAAGACAGTCT
  AAACTACGAAATACACCTTATCAGGCACCAGTTAGAAGACAAATGAAAGCTAAGCAACTCAGTGCACAA
  TCTTACGGTGTGACCAGTTCAACAGCTCGGCGAATATTGCAGTCTTTAGAGAAGATGTCAAGCCCTTTA
  GCGGATGCAAAAAGAATTCCATCCATTGTTTCTTCTCCTCTGAATTCTCCTCTTGATAGGAGTGGGATA
  GATATCACAGATTTTCAGGCCAAAAGAGAAAAGGTGGATTCTCAATATCCTCCTGTTCAGAGACTTATG
  ACCCCAAAGCCAGTTTCCATAGCAACAAATCGAAGTGTTTATTTTAAACCATCTCTGACTCCTTCTGGT
  GAATTCAGGAAGACTAATCAAAGAATAGATAACAAGTGCAGTACTGGATATGAAAAAAATATGACACCC
  GGACAAAATAGAGAACAACGAGAAAGTGGCTTTTCATATCCAAATTTCAGTTTGCCTGCAGCCAATGGT
  TTATCTTCTGGAGTAGGTGGTGGAGGTGGCAAGATGAGACGAGAAAGAACACGCTTTGTTGCTTCTAAA
  CCTCTGGAGGAGGAGAGACAAGGTCTCACCGTATTGCCCAAGCTGATCTCGAGCTCCTGCGCTCAAGCG
  ATCATCCCATCTTGGCCTCTCAAAGTGCTGAGATTACAGGAAATGGAAGTTCCAGTATTACCGAAAATC
  TCTCTACCGATCACCAGTTCTTCACTGCCTACCTTTAATTTTAGTTCCCCTGAGATCACAACTTCCTCT
  CCATCACCCATCAATTCGTCTCAAGCATTAACAAACAAGGTACAAATGACCTCTCCGAGCAGCACTGGC
  AGTCCCATGTTTAAATTTTCATCTCCAATCGTAAAATCTACTGAGGCAAATGTACTACCTCCATCATCT
  ATTGGATTTACATTTAGTGTGCCTGTTGCAAAAACAGCAGAACTTTCTGGTTCTAGTAGTACTTTAGAA
  CCAATTATAAGTAGTTCAGCTCATCATGTCACTACAGTGAACAGTACAAATTGTAAGAAGACACCACCT
  GAAGATTGTGAGGGTCCTTTTAGACCTGCAGAAATCCTGAAAGAAGGAAGTGTTCTAGATATTCTGAAA
  AGCCCTGGTTTCGCATCGCCGAAGATAGATTCTGTTGCTGCTCAGCCCACCGCAACAAGCCCAGTAGTT
  TATACAAGACCAGCAATAAGTAGCTTTTCTTCTAGTGGAATTGGGTTTGGGGAGAGTTTAAAAGCTGGG
  TCATCATGGCAGTGTGATACATGTCTACTCCAGAACAAAGTTACAGACAACAAATGCATAGCCTGTCAA
  GCAGCAAAATTGTCACCCAGAGATACTGCTAAACAGACTGGAATTGAAACACCAAATAAAAGTGGCAAA
  ACAACTCTTTCTGCATCAGGGACAGGCTTTGGAGACAAATTTAAACCAGTGATAGGCACTTGGGATTGT
  GATACCTGTTTAGTGCAAAATAAACCTGAAGCAATAAAATGTGTAGCCTGTGAAACACCGAAACCTGGA
  ACTTGTGTGAAGCGAGCCCTTACATTGACAGTGGTTTCGGAAAGTGCTGAGACTATGACTGCTTCATCT
  TCCAGCTGCACTGTAACCACTGGTACCTTAGGATTTGGAGATAAATTCAAAAGGCCCATTGGATCTTGG
  GAGTGTTCAGTATGCTGTGTTTCTAATAATGCAGAAGACAATAAGTGTGTGTCCTGTATGTCTGAGAAA
  CCAGGAAGTTCAGTACCTGCTTCAAGTAGCAGCACTGTACCTGTCTCTCTGCCTTCTGGAGGCTCTCTA
  GGATTGGAAAAGTTCAAGAAACCCGAGGGAAGCTGGGACTGTGAATTGTGCCTAGTGCAGAATAAGGCA
  GACTCTACCAAATGTTTGGCATGTGAAAGTGCAAAGCCAGGCACAAAATCTGGGTTTAAAGGCTTTGAC
  ACATCTTCCTCATCTTCGAACTCAGCAGCCTCCTCATCCTTCAAATTTGGTGTCTCATCATCCTCTTCT
  GGGCCTTCTCAGACTTTAACAAGCACTGGAAATTTTAAATTTGGAGATCAGGGAGGATTCAAAATAGGT
  GTGTCATCCGATTCTGGGTCTATAAACCCCATGAGTGAAGGCTTTAAATTTTCTAAACCAATAGGAGAT
  TTTAAATTTGGAGTTTCATCTGAATCTAAGCCCGAAGAAGTTAAAAAAGATAGTAAGAATGATAATTTT
  AAGTTTGGACTTTCTTCTGGTTTAAGCAACCCAGTTTCTTTAACTCCATTTCAATTTGGGGTATCTAAT
  CTTGGACAGGAAGAAAAGAAAGAGGAACTGCCCAAATCTTCCTCTGCAGGTTTTAGCTTTGGTACAGGT
  GTTATTAACTCCACCCCTGCTCCTGCTAACACCATAGTGACCTCTGAGAACAAGAGCAGCTTCAACCTT
  GGAACCATAGAAACCAAGAGTGCTTCAGTGGCTCCTTTCACATGTAAGACATCAGAAGCTAAAAAAGAA
  GAAATGCCTGCCACCAAAGGAGGATTCTCTTTTGGCAACGTGGAGCCTGCCTCTCTGCCATCTGCCTCA
  GTGTTTGTTTTGGGAAGGACAGAAGAGAAACAGCAAGAGCCTGTCACTTCTACTTCCCTAGTTTTTGGG
  AAGAAAGCTGACAATGAAGAGCCAAAGTGTCAACCAGTGTTTTCCTTTGGGAATTCAGAGCAAACCAAA
  GATGAGAATTCTTCAAAGTCCACATTTAGTTTTAGTATGACAAAACCATCTGAGAAGGAATCTGAACAG
  CCAGCAAAAGCCACTTTTGCCTTTGGAGCTCAAACTAGTACTACAGCTGATCAAGGTGCAGCAAAGCCA
  GTTTTTAGTTTCTTGAACAACAGTTCCTCTAGTTCAAGTACACCAGCCACTTCTGCTGGTGGTGGCATA
  TTTGGTAGTTCCACCTCTTCCTCCAATCCACCTGTGGCTACCTTTGTGTTTGGACAGTCCAGCAATCCT
  GTGAGCAGCTCTGCCTTTGGTAACACTGCTGAATCCAGCACCTCTCAGTCTTTGCTATTTTCTCAAGAT
  AGCAAACTAGCAACCACATCCAGCACAGGTACAGCTGTCACCCCATTTGTCTTTGGTCCAGGAGCCAGC
  AGTAATAATACTACCACCTCTGGTTTCGGCTTTGGAGCCACAACCACATCTAGCTCTGCAGGATCCTCC
  TTTGTATTTGGAACTGGACCCTCAGCACCATCTGCCAGTCCAGCATTTGGTGCTAACCAGACCCCAACA
  TTTGGACAAAGTCAAGGTGCCAGCCAGCCCAATCCCCCAGGCTTTGGATCTATATCATCTTCCACAGCA
  TTATTTCCCACTGGTTCTCAGCCTGCACCACCTACTTTTGGGACAGTGTCAAGCAGTAGCCAGCCCCCT
  GTGTTTGGACAGCAACCTAGTCAGTCTGCATTTGGCTCTGGAACAACTCCTAATTCTAGTTCGGCTTTC
  CAGTTTGGCAGCAGCACTACAAATTTCAACTTCACAAACAACAGTCCATCAGGAGTGTTCACATTTGGT
  GCAAATTCTAGCACACCTGCAGCCTCAGCCCAGCCTTCAGGCTCGGGGGGCTTTCCATTTAACCAGTCT
  CCAGCAGCATTTACAGTGGGGTCAAATGGGAAAAATGTGTTCTCTTCTTCTGGAACTTCATTCTCTGGT
  CGCAAGATAAAGACTGCTGTTAGACGCAGGAAA
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG240167
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MASGAGGVGGGGGGKIRTRRCHQGPIKPYQQGRQQHQGILSRVTESVKNIVPGWLQRYFNKNEDVCSCS
  TDTSEVPRWPENKEDHLVYADEESSNITDGRITPEPAVSNTEEPSTTSTASNYPDVLTRPSLHRSHLNF
  SMLESPALHCQPSTSSAFPIGSSGFSLVKEIKDSTSQHDDDNISTTSGFSSRASDKDITVSKNTSLPPL
  WSPEAERSHSLSQHTATSSKKPAFNLSAFGTLSPSLGNSSILKTSQLGDSPFYPGKTTYGGAAAAVRQS
  KLRNTPYQAPVRRQMKAKQLSAQSYGVTSSTARRILQSLEKMSSPLADAKRIPSIVSSPLNSPLDRSGI
  DITDFQAKREKVDSQYPPVQRLMTPKPVSIATNRSVYFKPSLTPSGEFRKTNQRIDNKCSTGYEKNMTP
  GQNREQRESGFSYPNFSLPAANGLSSGVGGGGGKMRRERTRFVASKPLEEERQGLTVLPKLISSSCAQA
  IIPSWPLKVLRLQEMEVPVLPKISLPITSSSLPTFNFSSPEITTSSPSPINSSQALTNKVQMTSPSSTG
  SPMFKFSSPIVKSTEANVLPPSSIGFTFSVPVAKTAELSGSSSTLEPIISSSAHHVTTVNSTNCKKTPP
  EDCEGPFRPAEILKEGSVLDILKSPGFASPKIDSVAAQPTATSPVVYTRPAISSFSSSGIGFGESLKAG
  SSWQCDTCLLQNKVTDNKCIACQAAKLSPRDTAKQTGIETPNKSGKTTLSASGTGFGDKFKPVIGTWDC
  DTCLVQNKPEAIKCVACETPKPGTCVKRALTLTVVSESAETMTASSSSCTVTTGTLGFGDKFKRPIGSW
  ECSVCCVSNNAEDNKCVSCMSEKPGSSVPASSSSTVPVSLPSGGSLGLEKFKKPEGSWDCELCLVQNKA
  DSTKCLACESAKPGTKSGFKGFDTSSSSSNSAASSSFKFGVSSSSSGPSQTLTSTGNFKFGDQGGFKIG
  VSSDSGSINPMSEGFKFSKPIGDFKFGVSSESKPEEVKKDSKNDNFKFGLSSGLSNPVSLTPFQFGVSN
  LGQEEKKEELPKSSSAGFSFGTGVINSTPAPANTIVTSENKSSFNLGTIETKSASVAPFTCKTSEAKKE
  EMPATKGGFSFGNVEPASLPSASVFVLGRTEEKQQEPVTSTSLVFGKKADNEEPKCQPVFSFGNSEQTK
  DENSSKSTFSFSMTKPSEKESEQPAKATFAFGAQTSTTADQGAAKPVFSFLNNSSSSSSTPATSAGGGI
  FGSSTSSSNPPVATFVFGQSSNPVSSSAFGNTAESSTSQSLLFSQDSKLATTSSTGTAVTPFVFGPGAS
  SNNTTTSGFGFGATTTSSSAGSSFVFGTGPSAPSASPAFGANQTPTFGQSQGASQPNPPGFGSISSSTA
  LFPTGSQPAPPTFGTVSSSSQPPVFGQQPSQSAFGSGTTPNSSSAFQFGSSTTNFNFTNNSPSGVFTFG
  ANSSTPAASAQPSGSGGFPFNQSPAAFTVGSNGKNVFSSSGTSFSGRKIKTAVRRRK
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001278209
• ORF Size: 4518 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.

Reference Data

• RefSeq: NM_001278209.1, NP_001265138.1
• RefSeq Size: 6030 bp
• RefSeq ORF: 4521 bp
• Locus ID: 9972
• Cytogenetics: 6p22.3
• Protein Families: Druggable Genome, Stem cell - Pluripotency
• MW: 157.8 kDa
• Gene Summary: Nuclear pore complexes regulate the transport of macromolecules between the nucleus and cytoplasm. They are composed of at least 100 different polypeptide subunits, many of which belong to the nucleoporin family. Nucleoporins are glycoproteins found in nuclear pores and contain characteristic pentapeptide XFXFG repeats as well as O-linked N-acetylglucosamine residues oriented towards the cytoplasm. The protein encoded by this gene has three distinct domains: a N-terminal region containing a pore targeting and an RNA-binding domain domain, a central region containing multiple zinc finger motifs, and a C-terminal region containing multiple XFXFG repeats. Alternative splicing results in multiple transcript variants of this gene. [provided by RefSeq, May 2013]