TET3 (NM_001287491) Human Tagged ORF Clone

CAT#: RG240211

  • TrueORF®

TET3 (GFP-tagged) - Human tet methylcytosine dioxygenase 3 (TET3)


Reconstitution Protocol

USD 1,920.00

2 Weeks*

Size
    • 10 ug

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Specifications

Product Data
Tag TurboGFP
Symbol TET3
Synonyms hCG_40738
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RG240211 representing NM_001287491.
Blue=ORF Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGAGCCAGTTTCAGGTGCCCCTGGCCGTCCAGCCGGACCTGCCAGGCCTTTATGACTTCCCTCAGCGC
CAGGTGATGGTAGGGAGCTTCCCGGGGTCTGGGCTCTCCATGGCTGGGAGTGAGTCCCAACTCCGAGGG
GGTGGAGATGGTCGAAAGAAACGGAAACGGTGTGGTACTTGTGAGCCCTGCCGGCGGCTGGAAAACTGT
GGCGCTTGCACTAGCTGTACCAACCGCCGCACGCACCAGATCTGCAAACTGCGAAAATGTGAGGTGCTG
AAGAAAAAAGTAGGGCTTCTCAAGGAGGTGGAAATAAAGGCTGGTGAAGGAGCCGGGCCGTGGGGACAA
GGAGCGGCTGTCAAGACAGGCTCAGAGCTCAGCCCAGTTGATGGACCTGTTCCAGGTCAGATGGACTCA
GGGCCAGTGTACCATGGGGACTCACGGCAGCTAAGCGCCTCAGGGGTGCCGGTCAATGGTGCTAGAGAG
CCCGCTGGACCCAGTCTGCTGGGGACTGGGGGTCCTTGGCGGGTAGACCAAAAGCCCGACTGGGAGGCT
GCCCCAGGCCCAGCTCATACTGCTCGCCTGGAAGATGCCCACGATCTGGTGGCCTTTTCGGCTGTGGCC
GAAGCTGTGTCCTCTTATGGGGCCCTTAGCACCCGGCTCTATGAAACCTTCAACCGTGAGATGAGTCGT
GAGGCTGGGAACAACAGCAGGGGACCCCGGCCAGGGCCTGAGGGCTGCTCTGCTGGCAGCGAAGACCTT
GACACACTGCAGACGGCCCTGGCCCTCGCGCGGCATGGTATGAAACCACCCAACTGCAACTGCGATGGC
CCAGAATGCCCTGACTACCTCGAGTGGCTGGAGGGGAAGATCAAGTCTGTGGTCATGGAAGGAGGGGAG
GAGCGGCCCAGGCTCCCAGGGCCTCTGCCTCCTGGTGAGGCCGGCCTCCCAGCACCAAGCACCAGGCCA
CTCCTCAGCTCAGAGGTGCCCCAGATCTCTCCCCAAGAGGGCCTGCCCCTGTCCCAGAGTGCCCTGAGC
ATTGCCAAGGAAAAAAACATCAGCTTGCAGACCGCCATTGCCATTGAGGCCCTCACACAGCTCTCCTCT
GCCCTCCCGCAGCCTTCTCATTCCACCCCCCAGGCTTCTTGCCCCCTTCCTGAGGCCTTGTCACCTCCT
GCCCCTTTCAGATCTCCCCAGTCTTACCTCCGGGCTCCCTCATGGCCTGTGGTTCCTCCTGAAGAGCAC
TCATCTTTTGCTCCTGATAGCTCTGCCTTCCCTCCAGCAACTCCTAGAACTGAGTTCCCTGAAGCCTGG
GGCACTGACACCCCTCCAGCAACGCCCCGGAGCTCCTGGCCCATGCCTCGCCCAAGCCCCGATCCCATG
GCTGAACTGGAGCAGTTGTTGGGCAGCGCCAGTGATTACATCCAGTCAGTATTCAAGCGGCCTGAGGCC
CTGCCTACCAAGCCCAAGGTCAAGGTGGAGGCACCCTCTTCCTCCCCGGCCCCGGCCCCATCCCCTGTA
CTTCAGAGGGAGGCTCCCACGCCATCCTCGGAGCCCGACACCCACCAGAAGGCCCAGACCGCCCTGCAG
CAGCACCTCCACCACAAGCGCAGCCTCTTCCTAGAACAGGTGCACGACACCTCCTTCCCTGCTCCTTCA
GAGCCTTCTGCTCCTGGCTGGTGGCCCCCACCAAGTTCACCTGTCCCACGGCTTCCAGACAGACCACCC
AAGGAGAAGAAGAAGAAGCTCCCAACACCAGCTGGAGGTCCCGTGGGAACGGAGAAAGCTGCCCCTGGG
ATCAAGCCCAGTGTCCGAAAGCCCATTCAGATCAAGAAGTCCAGGCCCCGGGAAGCACAGCCCCTCTTC
CCACCTGTCCGACAGATTGTCCTGGAAGGGCTTAGGTCCCCAGCCTCCCAGGAAGTGCAGGCTCATCCA
CCGGCCCCTCTGCCTGCCTCACAGGGCTCTGCTGTGCCCCTGCCCCCAGAACCTTCTCTTGCGCTATTT
GCACCTAGTCCCTCCAGGGACAGCCTGCTGCCCCCTACTCAGGAAATGAGGTCCCCCAGCCCCATGACA
GCCTTGCAGCCAGGCTCCACTGGCCCTCTTCCCCCTGCCGATGACAAGCTGGAAGAGCTCATCCGGCAG
TTTGAGGCTGAATTTGGAGATAGCTTTGGGCTTCCCGGCCCCCCTTCTGTGCCCATTCAGGACCCCGAG
AACCAGCAAACATGTCTCCCAGCCCCTGAGAGCCCCTTTGCTACCCGTTCCCCCAAGCAAATCAAGATT
GAGTCTTCGGGGGCTGTGACTGTGCTCTCAACCACCTGCTTCCATTCAGAGGAGGGAGGACAGGAGGCC
ACACCCACCAAGGCTGAGAACCCACTCACACCCACCCTCAGTGGCTTCTTGGAGTCACCTCTTAAGTAC
CTGGACACACCCACCAAGAGTCTGCTGGACACACCTGCCAAGAGAGCCCAGGCCGAGTTCCCCACCTGC
GATTGCGTCGAACAAATAGTGGAGAAAGATGAAGGTCCATATTATACTCACTTGGGATCTGGCCCCACG
GTCGCCTCTATCCGGGAACTCATGGAGGAGCGGTATGGAGAGAAGGGGAAAGCCATCCGGATCGAGAAG
GTCATCTACACGGGGAAGGAGGGAAAGAGCTCCCGCGGTTGCCCCATTGCAAAGTGGGTGATCCGCAGG
CACACGCTGGAGGAGAAGCTACTCTGCCTGGTGCGGCACCGGGCAGGCCACCACTGCCAGAACGCTGTG
ATCGTCATCCTCATCCTGGCCTGGGAGGGCATTCCCCGTAGCCTCGGAGACACCCTCTACCAGGAGCTC
ACCGACACCCTCCGGAAGTATGGGAACCCCACCAGCCGGAGATGCGGCCTCAACGATGACCGGACCTGC
GCTTGCCAAGGCAAAGACCCCAACACCTGTGGTGCCTCCTTCTCCTTTGGTTGTTCCTGGAGCATGTAC
TTCAACGGCTGCAAGTATGCTCGGAGCAAGACACCTCGCAAGTTCCGCCTCGCAGGGGACAATCCCAAA
GAGGAAGAAGTGCTCCGGAAGAGTTTCCAGGACCTGGCCACCGAAGTCGCTCCCCTGTACAAGCGACTG
GCCCCTCAGGCCTATCAGAACCAGGTGACCAACGAGGAAATAGCGATTGACTGCCGTCTGGGGCTGAAG
GAAGGACGGCCCTTCGCGGGGGTCACGGCCTGCATGGACTTCTGTGCCCACGCCCACAAGGACCAGCAT
AACCTCTACAATGGGTGCACCGTGGTCTGCACCCTGACCAAGGAAGACAATCGCTGCGTGGGCAAGATT
CCCGAGGATGAGCAGCTGCATGTTCTCCCCCTGTACAAGATGGCCAACACGGATGAGTTTGGTAGCGAG
GAGAACCAGAATGCAAAGGTGGGCAGCGGAGCCATCCAGGTGCTCACCGCCTTCCCCCGCGAGGTCCGA
CGCCTGCCCGAGCCTGCCAAGTCCTGCCGCCAGCGGCAGCTGGAAGCCAGAAAGGCAGCAGCCGAGAAG
AAGAAGATTCAGAAGGAGAAGCTGAGCACTCCGGAGAAGATCAAGCAGGAGGCCCTGGAGCTGGCGGGC
ATTACGTCGGACCCAGGCCTGTCTCTGAAGGGTGGATTGTCCCAGCAAGGCCTGAAGCCCTCCCTCAAG
GTGGAGCCGCAGAACCACTTCAGCTCCTTCAAGTACAGCGGCAACGCGGTGGTGGAGAGCTACTCGGTG
CTGGGCAACTGCCGGCCCTCCGACCCTTACAGCATGAACAGCGTGTACTCCTACCACTCCTACTATGCA
CAGCCCAGCCTGACCTCCGTCAATGGCTTCCACTCCAAGTACGCTCTCCCGTCTTTTAGCTACTATGGC
TTTCCATCCAGCAACCCCGTCTTCCCCTCTCAGTTCCTGGGTCCTGGTGCCTGGGGGCACAGTGGCAGC
AGTGGCAGTTTTGAGAAGAAGCCAGACCTCCACGCTCTGCACAACAGCCTGAGCCCGGCCTACGGTGGT
GCTGAGTTTGCCGAGCTGCCCAGCCAGGCTGTTCCCACAGACGCCCACCACCCCACTCCTCACCACCAG
CAGCCTGCGTACCCAGGCCCCAAGGAGTATCTGCTTCCCAAGGCCCCCCTACTCCACTCAGTGTCCAGG
GACCCCTCCCCCTTTGCCCAGAGCTCCAACTGCTACAACAGATCCATCAAGCAAGAGCCAGTAGACCCG
CTGACCCAGGCTGAGCCTGTGCCCAGAGACGCTGGCAAGATGGGCAAGACACCTCTGTCCGAGGTGTCT
CAGAATGGAGGACCCAGTCACCTTTGGGGACAGTACTCAGGAGGCCCAAGCATGTCCCCCAAGAGGACT
AACGGTGTGGGTGGCAGCTGGGGTGTGTTCTCGTCTGGGGAGAGTCCTGCCATCGTCCCTGACAAGCTC
AGTTCCTTTGGGGCCAGCTGCCTGGCCCCTTCCCACTTCACAGATGGCCAGTGGGGGCTGTTCCCCGGT
GAGGGGCAGCAGGCAGCTTCCCACTCTGGAGGACGGCTGCGAGGCAAACCGTGGAGCCCCTGCAAGTTT
GGGAACAGCACCTCGGCCTTGGCTGGGCCCAGCCTGACTGAGAAGCCGTGGGCGCTGGGGGCAGGGGAT
TTCAACTCGGCCCTGAAAGGTAGTCCTGGGTTCCAAGACAAGCTGTGGAACCCCATGAAAGGAGAGGAG
GGCAGGATTCCAGCCGCAGGGGCCAGCCAGCTGGACAGGGCCTGGCAGTCCTTTGGTCTGCCCCTGGGA
TCCAGCGAGAAGCTGTTTGGGGCTCTGAAGTCAGAGGAGAAGCTGTGGGACCCCTTCAGCCTGGAGGAG
GGGCCGGCTGAGGAGCCCCCCAGCAAGGGAGCGGTGAAGGAGGAGAAGGGCGGTGGTGGTGCGGAGGAG
GAAGAGGAGGAGCTGTGGTCGGACAGTGAACACAACTTCCTGGACGAGAACATCGGCGGCGTGGCCGTG
GCCCCAGCCCACGGCTCCATCCTCATCGAGTGTGCCCGGCGGGAGCTGCACGCCACCACGCCGCTTAAG
AAGCCCAACCGCTGCCACCCCACCCGCATCTCGCTGGTCTTCTACCAGCACAAGAACCTCAACCAGCCC
AACCACGGGCTGGCCCTCTGGGAAGCCAAGATGAAGCAGCTGGCGGAGAGGGCACGGGCACGGCAGGAG
GAGGCTGCCCGGCTGGGCCTGGGCCAGCAGGAGGCCAAGCTCTACGGGAAGAAGCGCAAGTGGGGGGGC
ACTGTGGTTGCTGAGCCCCAGCAGAAAGAGAAGAAGGGGGTCGTCCCCACCCGGCAGGCACTGGCTGTG
CCCACAGACTCGGCGGTCACCGTGTCCTCCTATGCCTACACGAAGGTCACTGGCCCCTACAGCCGCTGG
ATC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
>Peptide sequence encoded by RG240211
Blue=ORF Red=Cloning site Green=Tag(s)

MSQFQVPLAVQPDLPGLYDFPQRQVMVGSFPGSGLSMAGSESQLRGGGDGRKKRKRCGTCEPCRRLENC
GACTSCTNRRTHQICKLRKCEVLKKKVGLLKEVEIKAGEGAGPWGQGAAVKTGSELSPVDGPVPGQMDS
GPVYHGDSRQLSASGVPVNGAREPAGPSLLGTGGPWRVDQKPDWEAAPGPAHTARLEDAHDLVAFSAVA
EAVSSYGALSTRLYETFNREMSREAGNNSRGPRPGPEGCSAGSEDLDTLQTALALARHGMKPPNCNCDG
PECPDYLEWLEGKIKSVVMEGGEERPRLPGPLPPGEAGLPAPSTRPLLSSEVPQISPQEGLPLSQSALS
IAKEKNISLQTAIAIEALTQLSSALPQPSHSTPQASCPLPEALSPPAPFRSPQSYLRAPSWPVVPPEEH
SSFAPDSSAFPPATPRTEFPEAWGTDTPPATPRSSWPMPRPSPDPMAELEQLLGSASDYIQSVFKRPEA
LPTKPKVKVEAPSSSPAPAPSPVLQREAPTPSSEPDTHQKAQTALQQHLHHKRSLFLEQVHDTSFPAPS
EPSAPGWWPPPSSPVPRLPDRPPKEKKKKLPTPAGGPVGTEKAAPGIKPSVRKPIQIKKSRPREAQPLF
PPVRQIVLEGLRSPASQEVQAHPPAPLPASQGSAVPLPPEPSLALFAPSPSRDSLLPPTQEMRSPSPMT
ALQPGSTGPLPPADDKLEELIRQFEAEFGDSFGLPGPPSVPIQDPENQQTCLPAPESPFATRSPKQIKI
ESSGAVTVLSTTCFHSEEGGQEATPTKAENPLTPTLSGFLESPLKYLDTPTKSLLDTPAKRAQAEFPTC
DCVEQIVEKDEGPYYTHLGSGPTVASIRELMEERYGEKGKAIRIEKVIYTGKEGKSSRGCPIAKWVIRR
HTLEEKLLCLVRHRAGHHCQNAVIVILILAWEGIPRSLGDTLYQELTDTLRKYGNPTSRRCGLNDDRTC
ACQGKDPNTCGASFSFGCSWSMYFNGCKYARSKTPRKFRLAGDNPKEEEVLRKSFQDLATEVAPLYKRL
APQAYQNQVTNEEIAIDCRLGLKEGRPFAGVTACMDFCAHAHKDQHNLYNGCTVVCTLTKEDNRCVGKI
PEDEQLHVLPLYKMANTDEFGSEENQNAKVGSGAIQVLTAFPREVRRLPEPAKSCRQRQLEARKAAAEK
KKIQKEKLSTPEKIKQEALELAGITSDPGLSLKGGLSQQGLKPSLKVEPQNHFSSFKYSGNAVVESYSV
LGNCRPSDPYSMNSVYSYHSYYAQPSLTSVNGFHSKYALPSFSYYGFPSSNPVFPSQFLGPGAWGHSGS
SGSFEKKPDLHALHNSLSPAYGGAEFAELPSQAVPTDAHHPTPHHQQPAYPGPKEYLLPKAPLLHSVSR
DPSPFAQSSNCYNRSIKQEPVDPLTQAEPVPRDAGKMGKTPLSEVSQNGGPSHLWGQYSGGPSMSPKRT
NGVGGSWGVFSSGESPAIVPDKLSSFGASCLAPSHFTDGQWGLFPGEGQQAASHSGGRLRGKPWSPCKF
GNSTSALAGPSLTEKPWALGAGDFNSALKGSPGFQDKLWNPMKGEEGRIPAAGASQLDRAWQSFGLPLG
SSEKLFGALKSEEKLWDPFSLEEGPAEEPPSKGAVKEEKGGGGAEEEEEELWSDSEHNFLDENIGGVAV
APAHGSILIECARRELHATTPLKKPNRCHPTRISLVFYQHKNLNQPNHGLALWEAKMKQLAERARARQE
EAARLGLGQQEAKLYGKKRKWGGTVVAEPQQKEKKGVVPTRQALAVPTDSAVTVSSYAYTKVTGPYSRW
I

TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV

Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001287491
ORF Size 5385 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Reference Data
RefSeq NM_001287491.2
RefSeq Size 11405 bp
RefSeq ORF 5388 bp
Locus ID 200424
Cytogenetics 2p13.1
Protein Families Transcription Factors
MW 194.2 kDa
Gene Summary Members of the ten-eleven translocation (TET) gene family, including TET3, play a role in the DNA methylation process (Langemeijer et al., 2009 [PubMed 19923888]). [supplied by OMIM, Nov 2010]

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