PHF3 (NM_001290259) Human Tagged ORF Clone

CAT#: RG240224

PHF3 (GFP-tagged) - Human PHD finger protein 3 (PHF3), transcript variant 2

Specifications

Product Data

• Tag: TurboGFP
• Symbol: PHF3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG240224 representing NM_001290259.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGATGAAGGAGTTGTTAAAGAAAGTGGCAATGATACCATTGATGAAGAAGAACTGATTTTACCTAAC
  AGGAACTTAAGGGACAAGGTAGAAGAAAATTCAGTGAGATCTCCAAGAAAATCACCTCGTTTAATGGCA
  CAAGAACAAGTAAGAAGTTTGCGACAGAGCACTATTGCCAAGCGTTCAAATGCAGCACCATTAAGTAAC
  ACAAAAAAAGCATCTGGGAAGACTGTATCTACTGCTAAAGCAGGAGTGAAACAACCAGAAAGGAGTCAG
  GTTAAAGAAGAAGTATGTATGTCACTGAAACCTGAGTACCATAAGGAGAATAGAAGGTGCAGCCGAAAT
  AGCGGACAAATTGAAGTGGTACCTGAAGTATCAGTGTCTTCAAGTCATTCTTCAGTGTCATCTTGTCTT
  GAAATGAAGGATGAAGATGGATTAGATTCTAAGCATAAGTGTAATAATCCGGGAGAAATAGATGTGCCA
  TCTCATGAATTAAATTGTTCACTTCTTTCAGAGACTTGTGTTACTATTGGAGAAAAGAAAAATGAAGCT
  TTGATGGAATGTAAAGCCAAGCCTGTTGGTAGTCCATTGTTTAAGTTTTCAGATAAAGAAGAACATGAA
  CAAAATGATTCCATTTCAGGTAAAACGGGTGAGACTGTTGTTGAAGAAATGATAGCAACAAGAAAAGTT
  GAACAAGATTCAAAGGAGACAGTAAAATTATCCCATGAAGATGACCATATTCTTGAGGACGCTGGATCT
  TCTGATATTTCTAGTGATGCTGCTTGTACAAATCCAAATAAGACAGAAAACAGCCTTGTAGGTTTGCCT
  AGTTGTGTAGATGAAGTGACTGAATGTAATTTGGAATTGAAGGATACCATGGGTATTGCTGATAAAACT
  GAGAACACCCTTGAAAGAAATAAAATTGAACCGTTGGGTTATTGTGAAGATGCGGAGTCTAATAGGCAG
  TTGGAGAGCACTGAGTTTAATAAATCAAACTTAGAGGTGGTTGATACTAGTACTTTTGGACCGGAAAGT
  AATATCTTGGAAAATGCTATTTGTGATGTGCCTGACCAAAATTCAAAACAGTTGAATGCTATAGAAAGT
  ACTAAAATAGAGTCCCATGAAACAGCAAACCTTCAGGATGACAGAAACAGCCAGTCAAGTAGCGTTTCT
  TACTTAGAGTCAAAAAGTGTAAAATCCAAACATACAAAACCTGTAATTCATTCTAAGCAAAACATGACC
  ACAGATGCTCCGAAGAAAATTGTTGCAGCAAAGTATGAAGTAATACATAGCAAAACTAAAGTTAATGTC
  AAAAGTGTGAAACGAAATACTGATGTACCAGAATCTCAGCAAAATTTTCATAGGCCAGTCAAAGTCAGA
  AAAAAACAAATTGATAAGGAGCCAAAGATTCAGAGTTGCAATTCTGGGGTTAAATCTGTGAAAAACCAA
  GCTCATTCTGTACTGAAAAAAACATTACAGGATCAAACTTTAGTACAAATTTTCAAGCCCTTAACTCAT
  TCTTTGAGTGATAAGTCACACGCTCATCCTGGTTGCTTGAAAGAACCTCATCATCCTGCACAAACTGGA
  CATGTATCACATTCTAGCCAGAAACAGTGTCATAAGCCTCAGCAACAGGCCCCAGCAATGAAAACCAAT
  AGTCACGTGAAGGAAGAGCTTGAACACCCAGGCGTTGAGCATTTTAAGGAAGAGGATAAACTGAAACTG
  AAAAAACCTGAGAAGAACCTACAACCCCGCCAAAGAAGAAGCAGCAAAAGTTTTTCTTTAGATGAGCCA
  CCATTGTTCATTCCAGATAACATAGCTACCATAAGAAGAGAAGGCTCTGATCATAGCTCCTCATTTGAA
  AGCAAATATATGTGGACTCCCAGCAAGCAGTGTGGGTTTTGCAAAAAACCACATGGCAACAGGTTTATG
  GTTGGCTGTGGGAGATGTGATGACTGGTTTCATGGTGATTGTGTTGGGTTAAGTCTTTCTCAAGCACAG
  CAGATGGGCGAGGAAGACAAAGAATATGTCTGTGTAAAATGTTGTGCTGAAGAAGACAAAAAGACTGAA
  ATACTAGATCCAGATACTTTGGAAAACCAAGCTACAGTTGAATTCCATAGTGGAGATAAAACAATGGAG
  TGTGAAAAGCTTGGATTATCAAAACACACAACAAATGATAGAACCAAATATATAGATGATACAGTGAAG
  CACAAGGTCAAAATTTTAAAACGGGAGTCTGGTGAAGGCAGAAATTCATCAGACTGTAGAGATAATGAA
  ATTAAAAAATGGCAGCTAGCTCCTCTTCGTAAGATGGGACAACCAGTTTTACCTCGGAGATCCTCAGAA
  GAAAAAAGTGAAAAAATACCGAAAGAGTCTACAACTGTTACTTGCACAGGAGAAAAAGCTTCAAAACCA
  GGTACTCATGAGAAGCAAGAGATGAAAAAGAAGAAAGTTGAAAAAGGAGTGCTTAATGTACATCCTGCT
  GCTTCTGCTTCCAAGCCTTCTGCAGATCAGATCAGGCAAAGTGTCAGACATTCTCTCAAAGACATTCTT
  ATGAAGAGACTTACAGACTCAAATTTGAAGGTACCAGAGGAAAAGGCAGCAAAAGTTGCCACAAAAATT
  GAGAAAGAGCTTTTCTCTTTTTTTCGGGACACAGATGCTAAATATAAGAACAAATATAGAAGTTTGATG
  TTTAATTTGAAAGATCCTAAAAACAATATATTATTTAAAAAAGTACTGAAAGGAGAAGTAACTCCTGAT
  CATCTTATCAGAATGAGTCCAGAAGAACTAGCTTCTAAAGAGTTAGCTGCTTGGAGACGAAGAGAAAAC
  AGACATACCATAGAAATGATTGAGAAAGAGCAGAGAGAAGTGGAACGACGGCCAATCACCAAAATAACT
  CATAAAGGTGAAATAGAAATTGAGAGTGATGCCCCAATGAAAGAACAGGAAGCAGCCATGGAGATTCAG
  GAACCAGCCGCCAATAAGTCATTGGAGAAGCCAGAAGGATCTGAAAAACAAAAAGAGGAGGTTGACTCT
  ATGTCTAAAGATACCACTAGTCAACACAGACAGCATCTTTTTGATCTCAACTGCAAAATCTGCATAGGT
  CGAATGGCACCACCTGTAGATGATCTTTCTCCAAAAAAAGTAAAAGTTGTTGTAGGAGTAGCTCGCAAA
  CATTCAGACAATGAAGCAGAAAGTATAGCAGATGCATTATCTTCAACCTCAAATATTTTGGCTTCTGAA
  TTCTTTGAGGAGGAGAAACAGGAGTCTCCAAAGTCAACGTTCTCTCCTGCTCCACGTCCAGAGATGCCT
  GGAACTGTTGAAGTTGAGTCTACCTTTCTGGCTCGATTGAACTTCATCTGGAAAGGTTTTATCAACATG
  CCTTCTGTGGCAAAATTTGTTACCAAAGCCTATCCAGTATCTGGCTCCCCAGAATACCTGACAGAGGAC
  CTACCAGATAGTATTCAAGTAGGTGGCAGGATATCACCTCAGACAGTTTGGGATTATGTGGAAAAAATA
  AAAGCATCAGGAACCAAGGAAATTTGTGTGGTTCGCTTCACACCAGTAACTGAAGAAGATCAAATTTCT
  TATACTTTGCTCTTTGCATACTTCAGTAGCAGAAAGCGCTATGGAGTAGCTGCTAACAACATGAAGCAG
  GTTAAAGATATGTACCTTATTCCTTTGGGTGCCACAGATAAAATTCCACACCCTCTTGTGCCTTTTGAT
  GGACCTGGGCTTGAACTGCATAGACCTAATCTATTGTTGGGCTTAATTATTCGTCAGAAACTGAAGCGA
  CAGCACAGTGCCTGTGCTAGTACTAGTCATATAGCTGAGACTCCTGAAAGTGCACCACCAATAGCATTG
  CCACCTGATAAAAAAAGTAAAATAGAAGTTTCTACAGAAGAAGCACCAGAGGAAGAAAATGACTTTTTT
  AATTCTTTTACAACTGTATTACACAAGCAGAGAAATAAACCTCAGCAGAATCTTCAGGAAGACCTTCCA
  ACAGCAGTTGAACCTTTAATGGAAGTCACCAAACAGGAGCCACCAAAACCTTTAAGATTTCTTCCTGGC
  GTGTTGATTGGCTGGGAGAATCAACCTACTACTCTGGAATTAGCAAATAAACCTCTTCCTGTGGATGAT
  ATACTTCAAAGCCTTTTGGGCACCACTGGTCAAGTATATGACCAGGCCCAGTCAGTGATGGAACAAAAC
  ACTGTTAAAGAAATTCCATTTTTAAATGAGCAGACCAACTCAAAAATAGAGAAAACAGATAATGTGGAA
  GTAACTGATGGTGAAAACAAGGAGATAAAAGTTAAAGTAGATAATATTTCAGAATCTACAGATAAGTCA
  GCAGAAATAGAAACATCAGTAGTAGGGTCCTCTTCCATTTCTGCAGGGTCTTTGACGAGTCTTAGTCTC
  AGAGGTAAGCCACCAGATGTTTCTACAGAAGCATTTTTAACAAATTTATCAATTCAGTCAAAACAAGAG
  GAAACTGTGGAGAGTAAAGAGAAAACATTAAAAAGACAGCTTCAGGAAGATCAAGAGAATAATTTGCAA
  GATAACCAGACTTCAAATAGTTCTCCATGCAGATCTAATGTAGGAAAAGGAAACATAGATGGTAATGTG
  AGCTGTAGTGAAAACCTTGTTGCTAATACAGCGAGGTCTCCACAGTTTATCAACCTGAAAAGGGATCCT
  AGGCAAGCAGCAGGACGAAGTCAGCCTGTAACTACTTCAGAAAGCAAAGATGGAGATAGTTGCCGGAAT
  GGAGAAAAACACATGCTGCCTGGCCTGTCACACAACAAGGAGCACTTAACAGAACAAATCAATGTAGAG
  GAAAAGTTGTGTTCTGCAGAGAAAAACTCGTGTGTTCAGCAGAGTGACAATTTAAAAGTTGCACAAAAC
  TCACCATCAGTAGAAAACATACAGACTTCTCAAGCAGAACAAGCAAAACCCTTACAGGAGGATATTTTA
  ATGCAAAATATTGAAACTGTGCACCCATTTCGAAGAGGATCAGCAGTAGCGACATCTCATTTTGAAGTT
  GGAAACACATGTCCATCAGAATTTCCTTCTAAAAGCATCACCTTTACTTCCAGAAGCACCAGCCCCAGA
  ACAAGTACAAACTTTTCACCCATGAGGCCACAGCAGCCCAACCTTCAGCATCTCAAGTCTAGCCCACCT
  GGATTTCCATTTCCAGGGCCTCCTAATTTTCCCCCACAAAGCATGTTTGGATTTCCACCACATTTGCCA
  CCTCCATTACTTCCCCCTCCAGGCTTTGGCTTTGCTCAAAATCCCATGGTTCCCTGGCCACCTGTTGTT
  CATCTCCCAGGTCAGCCACAGCGTATGATGGGTCCTCTCTCACAAGCATCAAGGTATATAGGCCCGCAG
  AATTTTTACCAGGTTAAAGACATTCGGAGGCCAGAAAGGCGCCATAGTGACCCTTGGGGTAGGCAAGAC
  CAACAGCAACTGGATAGGCCATTTAATAGGGGTAAAGGGGACCGCCAGAGATTTTATAGTGATTCACAC
  CATTTGAAAAGAGAGCGACATGAAAAGGAATGGGAGCAAGAATCTGAAAGGCATAGACGCAGAGACAGA
  AGCCAAGACAAGGACAGAGACAGAAAAAGCAGGGAGGAAGGGCACAAAGATAAAGAGAGGGCACGGTTA
  TCACATGGTGATCGAGGAACAGATGGAAAAGCAAGCAGAGATAGTAGGAATGTAGACAAGAAGCCAGAT
  AAACCTAAAAGTGAAGACTATGAGAAGGACAAAGAACGAGAGAAAAGTAAACACAGAGAAGGAGAAAAG
  GACAGGGATAGGTACCACAAAGATAGGGACCACACTGACAGAACTAAAAGCAAAAGG
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG240224
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MDEGVVKESGNDTIDEEELILPNRNLRDKVEENSVRSPRKSPRLMAQEQVRSLRQSTIAKRSNAAPLSN
  TKKASGKTVSTAKAGVKQPERSQVKEEVCMSLKPEYHKENRRCSRNSGQIEVVPEVSVSSSHSSVSSCL
  EMKDEDGLDSKHKCNNPGEIDVPSHELNCSLLSETCVTIGEKKNEALMECKAKPVGSPLFKFSDKEEHE
  QNDSISGKTGETVVEEMIATRKVEQDSKETVKLSHEDDHILEDAGSSDISSDAACTNPNKTENSLVGLP
  SCVDEVTECNLELKDTMGIADKTENTLERNKIEPLGYCEDAESNRQLESTEFNKSNLEVVDTSTFGPES
  NILENAICDVPDQNSKQLNAIESTKIESHETANLQDDRNSQSSSVSYLESKSVKSKHTKPVIHSKQNMT
  TDAPKKIVAAKYEVIHSKTKVNVKSVKRNTDVPESQQNFHRPVKVRKKQIDKEPKIQSCNSGVKSVKNQ
  AHSVLKKTLQDQTLVQIFKPLTHSLSDKSHAHPGCLKEPHHPAQTGHVSHSSQKQCHKPQQQAPAMKTN
  SHVKEELEHPGVEHFKEEDKLKLKKPEKNLQPRQRRSSKSFSLDEPPLFIPDNIATIRREGSDHSSSFE
  SKYMWTPSKQCGFCKKPHGNRFMVGCGRCDDWFHGDCVGLSLSQAQQMGEEDKEYVCVKCCAEEDKKTE
  ILDPDTLENQATVEFHSGDKTMECEKLGLSKHTTNDRTKYIDDTVKHKVKILKRESGEGRNSSDCRDNE
  IKKWQLAPLRKMGQPVLPRRSSEEKSEKIPKESTTVTCTGEKASKPGTHEKQEMKKKKVEKGVLNVHPA
  ASASKPSADQIRQSVRHSLKDILMKRLTDSNLKVPEEKAAKVATKIEKELFSFFRDTDAKYKNKYRSLM
  FNLKDPKNNILFKKVLKGEVTPDHLIRMSPEELASKELAAWRRRENRHTIEMIEKEQREVERRPITKIT
  HKGEIEIESDAPMKEQEAAMEIQEPAANKSLEKPEGSEKQKEEVDSMSKDTTSQHRQHLFDLNCKICIG
  RMAPPVDDLSPKKVKVVVGVARKHSDNEAESIADALSSTSNILASEFFEEEKQESPKSTFSPAPRPEMP
  GTVEVESTFLARLNFIWKGFINMPSVAKFVTKAYPVSGSPEYLTEDLPDSIQVGGRISPQTVWDYVEKI
  KASGTKEICVVRFTPVTEEDQISYTLLFAYFSSRKRYGVAANNMKQVKDMYLIPLGATDKIPHPLVPFD
  GPGLELHRPNLLLGLIIRQKLKRQHSACASTSHIAETPESAPPIALPPDKKSKIEVSTEEAPEEENDFF
  NSFTTVLHKQRNKPQQNLQEDLPTAVEPLMEVTKQEPPKPLRFLPGVLIGWENQPTTLELANKPLPVDD
  ILQSLLGTTGQVYDQAQSVMEQNTVKEIPFLNEQTNSKIEKTDNVEVTDGENKEIKVKVDNISESTDKS
  AEIETSVVGSSSISAGSLTSLSLRGKPPDVSTEAFLTNLSIQSKQEETVESKEKTLKRQLQEDQENNLQ
  DNQTSNSSPCRSNVGKGNIDGNVSCSENLVANTARSPQFINLKRDPRQAAGRSQPVTTSESKDGDSCRN
  GEKHMLPGLSHNKEHLTEQINVEEKLCSAEKNSCVQQSDNLKVAQNSPSVENIQTSQAEQAKPLQEDIL
  MQNIETVHPFRRGSAVATSHFEVGNTCPSEFPSKSITFTSRSTSPRTSTNFSPMRPQQPNLQHLKSSPP
  GFPFPGPPNFPPQSMFGFPPHLPPPLLPPPGFGFAQNPMVPWPPVVHLPGQPQRMMGPLSQASRYIGPQ
  NFYQVKDIRRPERRHSDPWGRQDQQQLDRPFNRGKGDRQRFYSDSHHLKRERHEKEWEQESERHRRRDR
  SQDKDRDRKSREEGHKDKERARLSHGDRGTDGKASRDSRNVDKKPDKPKSEDYEKDKEREKSKHREGEK
  DRDRYHKDRDHTDRTKSKR
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001290259
• ORF Size: 5853 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.

Reference Data

• RefSeq: NM_001290259.2
• RefSeq Size: 8501 bp
• RefSeq ORF: 5856 bp
• Locus ID: 23469
• Cytogenetics: 6q12
• Protein Families: Transcription Factors
• MW: 220.3 kDa
• Gene Summary: This gene encodes a member of a PHD finger-containing gene family. This gene may function as a transcription factor and may be involved in glioblastomas development. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2014]