PHF3 (NM_001290259) Human Tagged ORF Clone
CAT#: RG240224
- TrueORF®
PHF3 (GFP-tagged) - Human PHD finger protein 3 (PHF3), transcript variant 2
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Specifications
Product Data | |
Tag | TurboGFP |
Symbol | PHF3 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG240224 representing NM_001290259.
Blue=ORF Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGATGAAGGAGTTGTTAAAGAAAGTGGCAATGATACCATTGATGAAGAAGAACTGATTTTACCTAAC AGGAACTTAAGGGACAAGGTAGAAGAAAATTCAGTGAGATCTCCAAGAAAATCACCTCGTTTAATGGCA CAAGAACAAGTAAGAAGTTTGCGACAGAGCACTATTGCCAAGCGTTCAAATGCAGCACCATTAAGTAAC ACAAAAAAAGCATCTGGGAAGACTGTATCTACTGCTAAAGCAGGAGTGAAACAACCAGAAAGGAGTCAG GTTAAAGAAGAAGTATGTATGTCACTGAAACCTGAGTACCATAAGGAGAATAGAAGGTGCAGCCGAAAT AGCGGACAAATTGAAGTGGTACCTGAAGTATCAGTGTCTTCAAGTCATTCTTCAGTGTCATCTTGTCTT GAAATGAAGGATGAAGATGGATTAGATTCTAAGCATAAGTGTAATAATCCGGGAGAAATAGATGTGCCA TCTCATGAATTAAATTGTTCACTTCTTTCAGAGACTTGTGTTACTATTGGAGAAAAGAAAAATGAAGCT TTGATGGAATGTAAAGCCAAGCCTGTTGGTAGTCCATTGTTTAAGTTTTCAGATAAAGAAGAACATGAA CAAAATGATTCCATTTCAGGTAAAACGGGTGAGACTGTTGTTGAAGAAATGATAGCAACAAGAAAAGTT GAACAAGATTCAAAGGAGACAGTAAAATTATCCCATGAAGATGACCATATTCTTGAGGACGCTGGATCT TCTGATATTTCTAGTGATGCTGCTTGTACAAATCCAAATAAGACAGAAAACAGCCTTGTAGGTTTGCCT AGTTGTGTAGATGAAGTGACTGAATGTAATTTGGAATTGAAGGATACCATGGGTATTGCTGATAAAACT GAGAACACCCTTGAAAGAAATAAAATTGAACCGTTGGGTTATTGTGAAGATGCGGAGTCTAATAGGCAG TTGGAGAGCACTGAGTTTAATAAATCAAACTTAGAGGTGGTTGATACTAGTACTTTTGGACCGGAAAGT AATATCTTGGAAAATGCTATTTGTGATGTGCCTGACCAAAATTCAAAACAGTTGAATGCTATAGAAAGT ACTAAAATAGAGTCCCATGAAACAGCAAACCTTCAGGATGACAGAAACAGCCAGTCAAGTAGCGTTTCT TACTTAGAGTCAAAAAGTGTAAAATCCAAACATACAAAACCTGTAATTCATTCTAAGCAAAACATGACC ACAGATGCTCCGAAGAAAATTGTTGCAGCAAAGTATGAAGTAATACATAGCAAAACTAAAGTTAATGTC AAAAGTGTGAAACGAAATACTGATGTACCAGAATCTCAGCAAAATTTTCATAGGCCAGTCAAAGTCAGA AAAAAACAAATTGATAAGGAGCCAAAGATTCAGAGTTGCAATTCTGGGGTTAAATCTGTGAAAAACCAA GCTCATTCTGTACTGAAAAAAACATTACAGGATCAAACTTTAGTACAAATTTTCAAGCCCTTAACTCAT TCTTTGAGTGATAAGTCACACGCTCATCCTGGTTGCTTGAAAGAACCTCATCATCCTGCACAAACTGGA CATGTATCACATTCTAGCCAGAAACAGTGTCATAAGCCTCAGCAACAGGCCCCAGCAATGAAAACCAAT AGTCACGTGAAGGAAGAGCTTGAACACCCAGGCGTTGAGCATTTTAAGGAAGAGGATAAACTGAAACTG AAAAAACCTGAGAAGAACCTACAACCCCGCCAAAGAAGAAGCAGCAAAAGTTTTTCTTTAGATGAGCCA CCATTGTTCATTCCAGATAACATAGCTACCATAAGAAGAGAAGGCTCTGATCATAGCTCCTCATTTGAA AGCAAATATATGTGGACTCCCAGCAAGCAGTGTGGGTTTTGCAAAAAACCACATGGCAACAGGTTTATG GTTGGCTGTGGGAGATGTGATGACTGGTTTCATGGTGATTGTGTTGGGTTAAGTCTTTCTCAAGCACAG CAGATGGGCGAGGAAGACAAAGAATATGTCTGTGTAAAATGTTGTGCTGAAGAAGACAAAAAGACTGAA ATACTAGATCCAGATACTTTGGAAAACCAAGCTACAGTTGAATTCCATAGTGGAGATAAAACAATGGAG TGTGAAAAGCTTGGATTATCAAAACACACAACAAATGATAGAACCAAATATATAGATGATACAGTGAAG CACAAGGTCAAAATTTTAAAACGGGAGTCTGGTGAAGGCAGAAATTCATCAGACTGTAGAGATAATGAA ATTAAAAAATGGCAGCTAGCTCCTCTTCGTAAGATGGGACAACCAGTTTTACCTCGGAGATCCTCAGAA GAAAAAAGTGAAAAAATACCGAAAGAGTCTACAACTGTTACTTGCACAGGAGAAAAAGCTTCAAAACCA GGTACTCATGAGAAGCAAGAGATGAAAAAGAAGAAAGTTGAAAAAGGAGTGCTTAATGTACATCCTGCT GCTTCTGCTTCCAAGCCTTCTGCAGATCAGATCAGGCAAAGTGTCAGACATTCTCTCAAAGACATTCTT ATGAAGAGACTTACAGACTCAAATTTGAAGGTACCAGAGGAAAAGGCAGCAAAAGTTGCCACAAAAATT GAGAAAGAGCTTTTCTCTTTTTTTCGGGACACAGATGCTAAATATAAGAACAAATATAGAAGTTTGATG TTTAATTTGAAAGATCCTAAAAACAATATATTATTTAAAAAAGTACTGAAAGGAGAAGTAACTCCTGAT CATCTTATCAGAATGAGTCCAGAAGAACTAGCTTCTAAAGAGTTAGCTGCTTGGAGACGAAGAGAAAAC AGACATACCATAGAAATGATTGAGAAAGAGCAGAGAGAAGTGGAACGACGGCCAATCACCAAAATAACT CATAAAGGTGAAATAGAAATTGAGAGTGATGCCCCAATGAAAGAACAGGAAGCAGCCATGGAGATTCAG GAACCAGCCGCCAATAAGTCATTGGAGAAGCCAGAAGGATCTGAAAAACAAAAAGAGGAGGTTGACTCT ATGTCTAAAGATACCACTAGTCAACACAGACAGCATCTTTTTGATCTCAACTGCAAAATCTGCATAGGT CGAATGGCACCACCTGTAGATGATCTTTCTCCAAAAAAAGTAAAAGTTGTTGTAGGAGTAGCTCGCAAA CATTCAGACAATGAAGCAGAAAGTATAGCAGATGCATTATCTTCAACCTCAAATATTTTGGCTTCTGAA TTCTTTGAGGAGGAGAAACAGGAGTCTCCAAAGTCAACGTTCTCTCCTGCTCCACGTCCAGAGATGCCT GGAACTGTTGAAGTTGAGTCTACCTTTCTGGCTCGATTGAACTTCATCTGGAAAGGTTTTATCAACATG CCTTCTGTGGCAAAATTTGTTACCAAAGCCTATCCAGTATCTGGCTCCCCAGAATACCTGACAGAGGAC CTACCAGATAGTATTCAAGTAGGTGGCAGGATATCACCTCAGACAGTTTGGGATTATGTGGAAAAAATA AAAGCATCAGGAACCAAGGAAATTTGTGTGGTTCGCTTCACACCAGTAACTGAAGAAGATCAAATTTCT TATACTTTGCTCTTTGCATACTTCAGTAGCAGAAAGCGCTATGGAGTAGCTGCTAACAACATGAAGCAG GTTAAAGATATGTACCTTATTCCTTTGGGTGCCACAGATAAAATTCCACACCCTCTTGTGCCTTTTGAT GGACCTGGGCTTGAACTGCATAGACCTAATCTATTGTTGGGCTTAATTATTCGTCAGAAACTGAAGCGA CAGCACAGTGCCTGTGCTAGTACTAGTCATATAGCTGAGACTCCTGAAAGTGCACCACCAATAGCATTG CCACCTGATAAAAAAAGTAAAATAGAAGTTTCTACAGAAGAAGCACCAGAGGAAGAAAATGACTTTTTT AATTCTTTTACAACTGTATTACACAAGCAGAGAAATAAACCTCAGCAGAATCTTCAGGAAGACCTTCCA ACAGCAGTTGAACCTTTAATGGAAGTCACCAAACAGGAGCCACCAAAACCTTTAAGATTTCTTCCTGGC GTGTTGATTGGCTGGGAGAATCAACCTACTACTCTGGAATTAGCAAATAAACCTCTTCCTGTGGATGAT ATACTTCAAAGCCTTTTGGGCACCACTGGTCAAGTATATGACCAGGCCCAGTCAGTGATGGAACAAAAC ACTGTTAAAGAAATTCCATTTTTAAATGAGCAGACCAACTCAAAAATAGAGAAAACAGATAATGTGGAA GTAACTGATGGTGAAAACAAGGAGATAAAAGTTAAAGTAGATAATATTTCAGAATCTACAGATAAGTCA GCAGAAATAGAAACATCAGTAGTAGGGTCCTCTTCCATTTCTGCAGGGTCTTTGACGAGTCTTAGTCTC AGAGGTAAGCCACCAGATGTTTCTACAGAAGCATTTTTAACAAATTTATCAATTCAGTCAAAACAAGAG GAAACTGTGGAGAGTAAAGAGAAAACATTAAAAAGACAGCTTCAGGAAGATCAAGAGAATAATTTGCAA GATAACCAGACTTCAAATAGTTCTCCATGCAGATCTAATGTAGGAAAAGGAAACATAGATGGTAATGTG AGCTGTAGTGAAAACCTTGTTGCTAATACAGCGAGGTCTCCACAGTTTATCAACCTGAAAAGGGATCCT AGGCAAGCAGCAGGACGAAGTCAGCCTGTAACTACTTCAGAAAGCAAAGATGGAGATAGTTGCCGGAAT GGAGAAAAACACATGCTGCCTGGCCTGTCACACAACAAGGAGCACTTAACAGAACAAATCAATGTAGAG GAAAAGTTGTGTTCTGCAGAGAAAAACTCGTGTGTTCAGCAGAGTGACAATTTAAAAGTTGCACAAAAC TCACCATCAGTAGAAAACATACAGACTTCTCAAGCAGAACAAGCAAAACCCTTACAGGAGGATATTTTA ATGCAAAATATTGAAACTGTGCACCCATTTCGAAGAGGATCAGCAGTAGCGACATCTCATTTTGAAGTT GGAAACACATGTCCATCAGAATTTCCTTCTAAAAGCATCACCTTTACTTCCAGAAGCACCAGCCCCAGA ACAAGTACAAACTTTTCACCCATGAGGCCACAGCAGCCCAACCTTCAGCATCTCAAGTCTAGCCCACCT GGATTTCCATTTCCAGGGCCTCCTAATTTTCCCCCACAAAGCATGTTTGGATTTCCACCACATTTGCCA CCTCCATTACTTCCCCCTCCAGGCTTTGGCTTTGCTCAAAATCCCATGGTTCCCTGGCCACCTGTTGTT CATCTCCCAGGTCAGCCACAGCGTATGATGGGTCCTCTCTCACAAGCATCAAGGTATATAGGCCCGCAG AATTTTTACCAGGTTAAAGACATTCGGAGGCCAGAAAGGCGCCATAGTGACCCTTGGGGTAGGCAAGAC CAACAGCAACTGGATAGGCCATTTAATAGGGGTAAAGGGGACCGCCAGAGATTTTATAGTGATTCACAC CATTTGAAAAGAGAGCGACATGAAAAGGAATGGGAGCAAGAATCTGAAAGGCATAGACGCAGAGACAGA AGCCAAGACAAGGACAGAGACAGAAAAAGCAGGGAGGAAGGGCACAAAGATAAAGAGAGGGCACGGTTA TCACATGGTGATCGAGGAACAGATGGAAAAGCAAGCAGAGATAGTAGGAATGTAGACAAGAAGCCAGAT AAACCTAAAAGTGAAGACTATGAGAAGGACAAAGAACGAGAGAAAAGTAAACACAGAGAAGGAGAAAAG GACAGGGATAGGTACCACAAAGATAGGGACCACACTGACAGAACTAAAAGCAAAAGG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC >Peptide sequence encoded by RG240224
Blue=ORF Red=Cloning site Green=Tag(s) MDEGVVKESGNDTIDEEELILPNRNLRDKVEENSVRSPRKSPRLMAQEQVRSLRQSTIAKRSNAAPLSN TKKASGKTVSTAKAGVKQPERSQVKEEVCMSLKPEYHKENRRCSRNSGQIEVVPEVSVSSSHSSVSSCL EMKDEDGLDSKHKCNNPGEIDVPSHELNCSLLSETCVTIGEKKNEALMECKAKPVGSPLFKFSDKEEHE QNDSISGKTGETVVEEMIATRKVEQDSKETVKLSHEDDHILEDAGSSDISSDAACTNPNKTENSLVGLP SCVDEVTECNLELKDTMGIADKTENTLERNKIEPLGYCEDAESNRQLESTEFNKSNLEVVDTSTFGPES NILENAICDVPDQNSKQLNAIESTKIESHETANLQDDRNSQSSSVSYLESKSVKSKHTKPVIHSKQNMT TDAPKKIVAAKYEVIHSKTKVNVKSVKRNTDVPESQQNFHRPVKVRKKQIDKEPKIQSCNSGVKSVKNQ AHSVLKKTLQDQTLVQIFKPLTHSLSDKSHAHPGCLKEPHHPAQTGHVSHSSQKQCHKPQQQAPAMKTN SHVKEELEHPGVEHFKEEDKLKLKKPEKNLQPRQRRSSKSFSLDEPPLFIPDNIATIRREGSDHSSSFE SKYMWTPSKQCGFCKKPHGNRFMVGCGRCDDWFHGDCVGLSLSQAQQMGEEDKEYVCVKCCAEEDKKTE ILDPDTLENQATVEFHSGDKTMECEKLGLSKHTTNDRTKYIDDTVKHKVKILKRESGEGRNSSDCRDNE IKKWQLAPLRKMGQPVLPRRSSEEKSEKIPKESTTVTCTGEKASKPGTHEKQEMKKKKVEKGVLNVHPA ASASKPSADQIRQSVRHSLKDILMKRLTDSNLKVPEEKAAKVATKIEKELFSFFRDTDAKYKNKYRSLM FNLKDPKNNILFKKVLKGEVTPDHLIRMSPEELASKELAAWRRRENRHTIEMIEKEQREVERRPITKIT HKGEIEIESDAPMKEQEAAMEIQEPAANKSLEKPEGSEKQKEEVDSMSKDTTSQHRQHLFDLNCKICIG RMAPPVDDLSPKKVKVVVGVARKHSDNEAESIADALSSTSNILASEFFEEEKQESPKSTFSPAPRPEMP GTVEVESTFLARLNFIWKGFINMPSVAKFVTKAYPVSGSPEYLTEDLPDSIQVGGRISPQTVWDYVEKI KASGTKEICVVRFTPVTEEDQISYTLLFAYFSSRKRYGVAANNMKQVKDMYLIPLGATDKIPHPLVPFD GPGLELHRPNLLLGLIIRQKLKRQHSACASTSHIAETPESAPPIALPPDKKSKIEVSTEEAPEEENDFF NSFTTVLHKQRNKPQQNLQEDLPTAVEPLMEVTKQEPPKPLRFLPGVLIGWENQPTTLELANKPLPVDD ILQSLLGTTGQVYDQAQSVMEQNTVKEIPFLNEQTNSKIEKTDNVEVTDGENKEIKVKVDNISESTDKS AEIETSVVGSSSISAGSLTSLSLRGKPPDVSTEAFLTNLSIQSKQEETVESKEKTLKRQLQEDQENNLQ DNQTSNSSPCRSNVGKGNIDGNVSCSENLVANTARSPQFINLKRDPRQAAGRSQPVTTSESKDGDSCRN GEKHMLPGLSHNKEHLTEQINVEEKLCSAEKNSCVQQSDNLKVAQNSPSVENIQTSQAEQAKPLQEDIL MQNIETVHPFRRGSAVATSHFEVGNTCPSEFPSKSITFTSRSTSPRTSTNFSPMRPQQPNLQHLKSSPP GFPFPGPPNFPPQSMFGFPPHLPPPLLPPPGFGFAQNPMVPWPPVVHLPGQPQRMMGPLSQASRYIGPQ NFYQVKDIRRPERRHSDPWGRQDQQQLDRPFNRGKGDRQRFYSDSHHLKRERHEKEWEQESERHRRRDR SQDKDRDRKSREEGHKDKERARLSHGDRGTDGKASRDSRNVDKKPDKPKSEDYEKDKEREKSKHREGEK DRDRYHKDRDHTDRTKSKR TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001290259 |
ORF Size | 5853 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reference Data | |
RefSeq | NM_001290259.2 |
RefSeq Size | 8501 bp |
RefSeq ORF | 5856 bp |
Locus ID | 23469 |
Cytogenetics | 6q12 |
Protein Families | Transcription Factors |
MW | 220.3 kDa |
Gene Summary | This gene encodes a member of a PHD finger-containing gene family. This gene may function as a transcription factor and may be involved in glioblastomas development. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2014] |
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