DOCK10 (NM_001290263) Human Tagged ORF Clone

CAT#: RG240237

DOCK10 (GFP-tagged) - Human dedicator of cytokinesis 10 (DOCK10), transcript variant DOCK10.2

Specifications

Product Data

• Tag: TurboGFP
• Symbol: DOCK10
• Synonyms: DRIP2; Nbla10300; ZIZ3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG240237 representing NM_001290263.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGAGTTTTCGAGGGAAGGTTTTTAAACGGGAGCCCAGTGAATTTTGGAAGAAGAGACGAACTGTGAGG
  AGAGTAAACCAAGAAGAAATCCACAGATTTAGTTCTCAAGAAAAGCCTAGGCTTCTCGAGCCTTTGGAT
  TATGAGACTGTCATTGAAGAACTTGAAAAGACCTACCGGAATGATCCTCTTCAAGATCTCTTGTTCTTC
  CCCAGTGATGACTTTTCAGCAGCCACAGTTTCCTGGGATATCCGCACGTTGTACTCAACAGTACCTGAA
  GATGCAGAGCACAAGGCAGAAAATTTACTGGTTAAGGAGGCTTGTAAATTTTATAGTTCCCAGTGGCAT
  GTGGTAAACTACAAATATGAACAATATTCTGGAGACATTCGACAGCTACCCCGAGCAGAATACAAACCA
  GAGAAGCTTCCTTCACATTCCTTTGAGATTGACCATGAAGATGCTGATAAGGATGAAGATACCACTTCC
  CACTCGTCTTCCAAGGGGGGTGGAGGAGCGGGAGGAACTGGTGTTTTCAAGTCCGGCTGGCTCTACAAG
  GGGAATTTTAACAGCACCGTGAACAACACCGTTACTGTTCGGTCATTCAAAAAGCGCTACTTCCAGCTG
  ACTCAGTTACCAGATAACTCCTACATTATGAACTTTTACAAAGATGAGAAAATATCCAAAGAACCCAAA
  GGATGCATCTTTTTGGATTCCTGTACAGGAGTGGTGCAGAATAACAGACTAAGAAAATATGCCTTTGAA
  TTGAAAATGAATGATCTGACCTATTTTGTGCTGGCAGCTGAAACAGAGTCAGATATGGATGAATGGATC
  CACACCCTCAACCGCATTCTGCAAATCAGTCCTGAGGGGCCCCTCCAAGGGAGGAGGAGCACAGAGCTC
  ACTGATCTGGGTCTGGATTCGCTGGATAATTCTGTAACTTGTGAATGCACGCCAGAGGAAACAGATTCT
  TCAGAGAACAACCTACACGCAGACTTTGCAAAGTACCTCACAGAAACAGAAGATACTGTAAAAACAACT
  CGAAACATGGAGAGGCTAAATCTGTTCTCTCTAGATCCAGACATAGATACCTTGAAACTTCAAAAAAAA
  GATCTCTTGGAACCTGAGTCTGTGATCAAACCATTTGAAGAAAAAGCTGCCAAGAGAATCATGATCATC
  TGTAAAGCCCTCAACTCAAATCTTCAGGGATGTGTTACGGAGAATGAAAATGATCCGATAACGAATATT
  GAGCCTTTTTTTGTGAGTGTGGCACTTTATGACCTCAGAGACAGCAGGAAGATTTCTGCTGATTTTCAT
  GTGGATCTAAACCATGCTGCTGTCAGACAGATGCTCTTGGGGGCTTCTGTGGCTTTGGAAAATGGCAAC
  ATCGACACCATCACTCCAAGACAATCAGAAGAACCTCACATCAAGGGACTTCCAGAGGAATGGCTAAAA
  TTTCCAAAGCAGGCTGTATTTTCTGTAAGCAATCCACATTCTGAAATTGTTTTGGTGGCCAAAATCGAA
  AAAGTCTTGATGGGAAACATTGCAAGTGGTGCCGAACCTTATATTAAGAACCCAGACTCCAACAAGTAT
  GCACAAAAGATACTAAAATCCAACAGACAATTCTGCAGCAAATTGGGAAAATACCGTATGCCTTTTGCT
  TGGGCAGTAAGATCAGTATTTAAGGACAACCAGGGAAATGTGGACAGAGACTCAAGATTTTCACCATTG
  TTTAGACAAGAAAGTAGCAAGATTTCAACTGAGGACCTAGTTAAACTAGTATCAGATTATAGAAGGGCC
  GACAGAATAAGCAAAATGCAGACCATTCCTGGAAGCCTGGATATTGCTGTTGACAACGTTCCCTTGGAG
  CATCCAAATTGTGTAACATCGTCCTTTATCCCTGTCAAGCCTTTCAACATGATGGCTCAAACAGAACCC
  ACAGTGGAGGTGGAAGAATTTGTTTACGATTCAACAAAGTATTGTCGGCCTTACAGAGTATATAAAAAT
  CAAATTTATATTTACCCCAAACACCTCAAGTATGATAGCCAGAAATGCTTCAACAAGGCACGGAATATA
  ACTGTGTGCATTGAATTCAAAAATTCAGATGAAGAAAGTGCCAAGCCCCTGAAGTGTATTTATGGAAAA
  CCTGGAGGGCCCCTCTTCACCTCAGCCGCCTACACAGCAGTTCTGCACCACTCTCAGAATCCGGATTTC
  TCAGATGAGGTGAAAATTGAGCTACCAACACAACTCCATGAGAAACACCATATTTTGTTTTCTTTTTAT
  CACGTCACCTGTGACATCAATGCAAAAGCTAATGCCAAAAAGAAGGAGGCTCTGGAAACGTCAGTTGGA
  TATGCTTGGCTTCCTCTGATGAAACACGATCAGATAGCTTCTCAAGAGTACAACATCCCAATAGCAACA
  AGTCTGCCTCCTAATTATTTAAGCTTTCAAGATTCTGCAAGTGGAAAGCATGGTGGGAGTGACATTAAA
  TGGGTTGATGGTGGCAAACCACTTTTCAAAGTGTCGACATTTGTTGTATCAACAGTAAATACTCAGGAT
  CCACATGTGAATGCATTTTTCCAAGAGTGCCAAAAAAGAGAGAAAGATATGTCTCAGTCACCTACCTCA
  AATTTCATCCGCTCTTGTAAGAACTTATTGAATGTGGAAAAGATTCATGCAATCATGAGTTTTCTGCCT
  ATAATTTTGAATCAGCTCTTCAAAGTTCTGGTACAGAATGAGGAAGATGAAATAACTACAACTGTCACC
  AGGGTTCTGACCGACATTGTGGCCAAGTGCCATGAGGAGCAGCTGGATCATTCTGTCCAGTCATATATT
  AAGTTCGTGTTCAAGACCAGGGCATGCAAGGAGAGGACTGTACATGAGGAACTGGCTAAAAATGTGACT
  GGTCTTTTGAAATCAAATGACTCAACAACAGTAAAGCATGTCCTAAAGCATTCCTGGTTCTTCTTTGCA
  ATTATCCTAAAATCGATGGCACAGCACTTGATTGACACAAATAAAATCCAGCTTCCCCGGCCTCAGAGA
  TTTCCTGAATCTTACCAAAATGAATTGGACAATCTTGTCATGGTCCTATCCGACCATGTGATTTGGAAA
  TACAAGGATGCACTTGAAGAAACAAGAAGGGCAAACCACAGCGTTGCCAGATTTCTCAAGCGCTGCTTT
  ACATTTATGGACCGAGGGTATGTGTTTAAGATGGTCAACAATTACATCAGCATGTTCTCCTCCGGTGAC
  CTTAAGACCTTGTGCCAGTATAAATTTGATTTTCTTCAAGAAGTATGTCAACATGAACACTTTATCCCT
  TTGTGTCTGCCCATAAGATCAGCAAACATTCCAGATCCTTTGACACCTTCAGAATCGACTCAAGAGTTA
  CATGCATCAGATATGCCTGAATATTCAGTCACAAATGAATTTTGTCGCAAACACTTCTTAATCGGAATT
  CTGCTCCGAGAAGTTGGCTTTGCCCTGCAGGAAGACCAAGATGTCAGACACTTAGCTTTAGCTGTCCTA
  AAAAATCTAATGGCTAAGCATTCATTTGATGATCGATACAGAGAGCCAAGAAAGCAGGCCCAGATAGCA
  AGTTTATACATGCCCCTGTACGGCATGCTCCTGGACAATATGCCAAGGATTTATCTGAAGGACCTGTAT
  CCTTTTACTGTCAATACATCTAATCAGGGGTCTAGAGATGATCTAAGCACCAATGGAGGATTTCAAAGC
  CAGACAGCTATCAAACATGCAAACTCTGTGGATACATCATTTTCTAAAGATGTTTTAAATTCCATAGCA
  GCATTTTCATCAATAGCTATTTCTACAGTAAACCATGCTGACTCCAGAGCATCTTTAGCAAGTCTTGAC
  TCCAATCCAAGTACCAATGAGAAGAGCAGTGAGAAGACGGACAACTGTGAAAAGATCCCAAGACCCTTG
  TCTTTGATTGGCTCAACTCTTCGATTTGACAAGTTAGATCAAGCAGAAACCAGGAGTCTCCTGATGTGT
  TTTCTTCACATTATGAAAACGATTTCGTACGAGACTCTGATTGCCTACTGGCAGAGAGCTCCCAGCCCA
  GAGGTGTCCGACTTCTTCAGCATCTTGGACGTTTGTCTTCAAAATTTCAGATACCTAGGAAAACGCAAC
  ATAATAAGAAAAATTGCTGCTGCATTTAAATTTGTGCAGTCCACCCAGAACAATGGAACTCTCAAAGGA
  TCCAATCCTTCCTGCCAGACATCAGGTCTCTTGTCACAATGGATGCACTCCACTTCCAGTCATGAAGGC
  CATAAGCAGCACAGATCACAAACTTTACCTATAATTCGAGGCAAAAATGCACTTTCTAACCCCAAACTC
  TTACAGATGTTAGACAATACCATGACCAGCAACTCCAATGAAATAGACATCGTGCATCATGTAGACACT
  GAGGCCAATATAGCTACGGAGGTTTGCCTCACTATTCTGGACCTGTTATCCCTCTTCACACAGACTCAT
  CAGAGACAACTCCAACAATGTGACTGTCAAAATTCATTGATGAAAAGGGTCTTTGATACCTACATGCTC
  TTTTTCCAAGTCAATCAGTCAGCCACAGCGCTGAAGCATGTGTTTGCCTCCTTGAGACTGTTTGTATGC
  AAGTTTCCTTCAGCGTTCTTTCAAGGGCCTGCTGACCTCTGTGGATCATTCTGTTACGAAGTCCTAAAA
  TGCTGTAACCACAGGTCACGGTCAACTCAGACAGAAGCCTCAGCCCTTCTGTACTTTTTCATGAGGAAG
  AATTTTGAATTTAACAAGCAGAAGTCAATTGTCCGGTCCCACTTACAACTCATCAAAGCTGTGAGCCAG
  TTAATAGCCGATGCTGGGATTGGAGGCTCTCGGTTTCAACATTCGCTTGCAATTACCAATAATTTCGCC
  AATGGAGATAAGCAAATGAAAAACAGCAATTTCCCAGCAGAGGTGAAGGACCTGACTAAGCGTATAAGG
  ACTGTTTTGATGGCCACAGCTCAGATGAAGGAGCACGAGAAGGACCCCGAGATGCTGGTGGATCTCCAG
  TACAGCCTGGCAAACTCCTACGCAAGCACTCCTGAACTACGCAGGACCTGGCTGGAAAGTATGGCCAAG
  ATTCATGCCAGAAACGGAGATTTATCTGAGGCTGCCATGTGTTACATCCATATTGCTGCTCTCATTGCA
  GAGTATCTGAAAAGAAAGGGTTACTGGAAAGTGGAAAAGATTTGCACAGCATCCCTGCTCTCGGAGGAT
  ACCCACCCCTGTGATAGCAACTCATTACTAACAACTCCCAGTGGAGGAAGCATGTTCTCTATGGGATGG
  CCAGCTTTTTTGAGCATTACACCAAACATTAAGGAAGAAGGAGCGATGAAAGAGGATTCTGGAATGCAA
  GATACACCATACAATGAGAATATCCTGGTGGAGCAGCTATACATGTGTGTGGAGTTTCTCTGGAAGTCT
  GAGCGATATGAACTCATTGCTGATGTCAACAAGCCCATCATTGCTGTCTTTGAGAAACAACGAGACTTC
  AAAAAATTGTCAGATCTCTACTACGACATTCATCGGTCATATCTGAAAGTGGCAGAGGTGGTGAATTCG
  GAGAAGCGGCTGTTTGGTCGCTACTATCGTGTGGCATTTTATGGGCAGGGCTTTTTTGAAGAAGAAGAA
  GGTAAAGAGTATATTTATAAAGAGCCTAAGCTGACAGGTCTGTCCGAGATTTCCCAAAGATTACTCAAG
  CTCTATGCAGATAAATTTGGAGCAGACAATGTGAAGATAATCCAGGATTCCAACAAGGTAAACCCCAAG
  GATTTGGACCCCAAATATGCCTACATCCAGGTGACCTATGTGACGCCGTTCTTTGAGGAAAAGGAAATC
  GAAGACCGGAAGACAGATTTCGAAATGCACCACAACATCAACCGCTTTGTCTTCGAGACACCCTTCACG
  CTGTCGGGCAAGAAGCACGGTGGGGTGGCGGAGCAGTGCAAGCGGCGGACGATCCTGACAACGAGTCAC
  CTGTTCCCCTACGTGAAGAAGAGAATACAAGTAATTAGCCAATCGAGCACAGAACTGAATCCAATTGAA
  GTGGCAATTGACGAGATGTCCAAGAAGGTTTCTGAGCTTAATCAGCTTTGCACAATGGAAGAAGTGGAC
  ATGATCAGACTGCAGCTCAAACTGCAAGGAAGTGTCAGCGTGAAGGTTAATGCTGGGCCAATGGCCTAT
  GCACGAGCTTTTCTTGAAGAAACCAATGCAAAGAAGTACCCTGACAACCAAGTAAAGCTTTTGAAGGAG
  ATCTTCAGGCAATTTGCAGATGCATGTGGGCAGGCCCTTGACGTGAATGAGCGCCTCATCAAAGAGGAC
  CAGCTGGAGTACCAGGAAGAACTGAGGTCCCACTACAAGGACATGCTCAGCGAACTCTCCACAGTCATG
  AATGAGCAGATTACGGGCAGGGACGACCTGTCAAAGCGCGGAGTGGACCAAACCTGCACTCGAGTAATT
  AGCAAAGCAACTCCGGCCCTACCCACGGTCTCCATCTCATCTAGTGCTGAAGTC
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG240237
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MSFRGKVFKREPSEFWKKRRTVRRVNQEEIHRFSSQEKPRLLEPLDYETVIEELEKTYRNDPLQDLLFF
  PSDDFSAATVSWDIRTLYSTVPEDAEHKAENLLVKEACKFYSSQWHVVNYKYEQYSGDIRQLPRAEYKP
  EKLPSHSFEIDHEDADKDEDTTSHSSSKGGGGAGGTGVFKSGWLYKGNFNSTVNNTVTVRSFKKRYFQL
  TQLPDNSYIMNFYKDEKISKEPKGCIFLDSCTGVVQNNRLRKYAFELKMNDLTYFVLAAETESDMDEWI
  HTLNRILQISPEGPLQGRRSTELTDLGLDSLDNSVTCECTPEETDSSENNLHADFAKYLTETEDTVKTT
  RNMERLNLFSLDPDIDTLKLQKKDLLEPESVIKPFEEKAAKRIMIICKALNSNLQGCVTENENDPITNI
  EPFFVSVALYDLRDSRKISADFHVDLNHAAVRQMLLGASVALENGNIDTITPRQSEEPHIKGLPEEWLK
  FPKQAVFSVSNPHSEIVLVAKIEKVLMGNIASGAEPYIKNPDSNKYAQKILKSNRQFCSKLGKYRMPFA
  WAVRSVFKDNQGNVDRDSRFSPLFRQESSKISTEDLVKLVSDYRRADRISKMQTIPGSLDIAVDNVPLE
  HPNCVTSSFIPVKPFNMMAQTEPTVEVEEFVYDSTKYCRPYRVYKNQIYIYPKHLKYDSQKCFNKARNI
  TVCIEFKNSDEESAKPLKCIYGKPGGPLFTSAAYTAVLHHSQNPDFSDEVKIELPTQLHEKHHILFSFY
  HVTCDINAKANAKKKEALETSVGYAWLPLMKHDQIASQEYNIPIATSLPPNYLSFQDSASGKHGGSDIK
  WVDGGKPLFKVSTFVVSTVNTQDPHVNAFFQECQKREKDMSQSPTSNFIRSCKNLLNVEKIHAIMSFLP
  IILNQLFKVLVQNEEDEITTTVTRVLTDIVAKCHEEQLDHSVQSYIKFVFKTRACKERTVHEELAKNVT
  GLLKSNDSTTVKHVLKHSWFFFAIILKSMAQHLIDTNKIQLPRPQRFPESYQNELDNLVMVLSDHVIWK
  YKDALEETRRANHSVARFLKRCFTFMDRGYVFKMVNNYISMFSSGDLKTLCQYKFDFLQEVCQHEHFIP
  LCLPIRSANIPDPLTPSESTQELHASDMPEYSVTNEFCRKHFLIGILLREVGFALQEDQDVRHLALAVL
  KNLMAKHSFDDRYREPRKQAQIASLYMPLYGMLLDNMPRIYLKDLYPFTVNTSNQGSRDDLSTNGGFQS
  QTAIKHANSVDTSFSKDVLNSIAAFSSIAISTVNHADSRASLASLDSNPSTNEKSSEKTDNCEKIPRPL
  SLIGSTLRFDKLDQAETRSLLMCFLHIMKTISYETLIAYWQRAPSPEVSDFFSILDVCLQNFRYLGKRN
  IIRKIAAAFKFVQSTQNNGTLKGSNPSCQTSGLLSQWMHSTSSHEGHKQHRSQTLPIIRGKNALSNPKL
  LQMLDNTMTSNSNEIDIVHHVDTEANIATEVCLTILDLLSLFTQTHQRQLQQCDCQNSLMKRVFDTYML
  FFQVNQSATALKHVFASLRLFVCKFPSAFFQGPADLCGSFCYEVLKCCNHRSRSTQTEASALLYFFMRK
  NFEFNKQKSIVRSHLQLIKAVSQLIADAGIGGSRFQHSLAITNNFANGDKQMKNSNFPAEVKDLTKRIR
  TVLMATAQMKEHEKDPEMLVDLQYSLANSYASTPELRRTWLESMAKIHARNGDLSEAAMCYIHIAALIA
  EYLKRKGYWKVEKICTASLLSEDTHPCDSNSLLTTPSGGSMFSMGWPAFLSITPNIKEEGAMKEDSGMQ
  DTPYNENILVEQLYMCVEFLWKSERYELIADVNKPIIAVFEKQRDFKKLSDLYYDIHRSYLKVAEVVNS
  EKRLFGRYYRVAFYGQGFFEEEEGKEYIYKEPKLTGLSEISQRLLKLYADKFGADNVKIIQDSNKVNPK
  DLDPKYAYIQVTYVTPFFEEKEIEDRKTDFEMHHNINRFVFETPFTLSGKKHGGVAEQCKRRTILTTSH
  LFPYVKKRIQVISQSSTELNPIEVAIDEMSKKVSELNQLCTMEEVDMIRLQLKLQGSVSVKVNAGPMAY
  ARAFLEETNAKKYPDNQVKLLKEIFRQFADACGQALDVNERLIKEDQLEYQEELRSHYKDMLSELSTVM
  NEQITGRDDLSKRGVDQTCTRVISKATPALPTVSISSSAEV
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001290263
• ORF Size: 6540 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.

Reference Data

• RefSeq: NM_001290263.2
• RefSeq Size: 7288 bp
• RefSeq ORF: 6543 bp
• Locus ID: 55619
• Cytogenetics: 2q36.2
• MW: 249.9 kDa
• Gene Summary: This gene encodes a member of the dedicator of cytokinesis protein family. Members of this family are guanosine nucleotide exchange factors for Rho GTPases and defined by the presence of conserved DOCK-homology regions. The encoded protein belongs to the D (or Zizimin) subfamily of DOCK proteins, which also contain an N-terminal pleckstrin homology domain. Alternatively spliced transcript variants that encode different isoforms have been described. [provided by RefSeq, Mar 2014]