Lig1 (NM_001024268) Rat Untagged Clone

CAT#: RN200286

Lig1 (untagged ORF) - Rat ligase I, DNA, ATP-dependent (Lig1), transcript variant 1, (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Lig1
• Synonyms: MGC105416
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN200286 representing NM_001024268
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGAGAAGTATTATGTCATTTTTCCAACCCACAACAACAGAAGGTAAAGCGAAGAAGCCAGAGAAGG
  AGATACCCAGCAGCATCAGAGAGAAGGAACCCCCTCCAAAGGTGGCATTGAAGGAGAGGAATCGAGCAGT
  GCCTGAGAGTGATTCTCCAGTGAAGAGGCCAGGAAGGAAGGTGGCCCAGGTTCTGAGCAGTGAAGGGGAG
  GACGAAGATGAAGCCCCTGGCACCCCCCAAGTCCAAAAGCCTGTGTCAGACTCCAAACAGAGCTCTCCTC
  CCAGCCCTGACTCCTGTCCTGAGAACAGCAGCCCCTCCATGGACATCTCCCCATCAGGATTCCCAAAGCG
  CCGAACTGCACGGAAGCAGCTCCCAAAACGGACAATTCAAGACACTCTTGAGGAGCACAATGAAGACAAA
  GACAAAGCAGCTAAGAAAAGGAAAAAAGAAGATACACAGACCCCAACAGAAAGCCTCACAGAGGCTGAAG
  AAGTAAAACAGAAGGAAGAACAGGTGGAGGACCAGCCCACAGTACCCCCTGAGCCCACAGAGTCCCCTGA
  GTCAGTAACCCTCACAAAGACAGAGAACATTCCAATGTGTAAGGCAGGTGTGAAACAGAAGCCTCAGGAA
  GAAGAACAGAGCAAGCCTCCTGCCAGGGGTGCCAAGCCACTGAGCAGTTTCTTCACACCCCGAAAACCAG
  CAGTGAAAACTGAAGTCAAACAAGAAGAGTCAGACACTCCAAGGAAGGAAGAGACCAAGGGAGCCCCGGA
  TCCAACCAATTATAATCCTTCCAAGAGTAACTACCATCCCATTGAAGATGCCTGCTGGAAACATGGCCAA
  AAGGTTCCTTTTCTTGCTGTGGCCCGGACATTTGAGAAGATTGAGGAGGTGTCTGCTCGGCTTAAGATGG
  TGGAGACACTGAGCAACCTGCTGCGCTCCGTGGTGGCCCTGTCCCCAACAGACCTGCTCCCTGTTCTCTA
  TCTCAGCCTCAACCGCCTTGGGCCGCCTCAGCAGGGTCTAGAACTGGGTGTTGGTGATGGTGTCCTCCTC
  AAGGCAGTTGCCCAGGCCACAGGTCGTCAGCTGGAGTCCATCCGGGCTGAGGTAGCTGAGAAGGGGGATG
  TGGGGCTAGTGGCGGAGAACAGCCGAAGCACTCAGAGGCTCATGCTGCCTCCACCTCCACTTACTGTTTC
  TGGAGTCTTTACCAAATTCTGTGACATTGCCCGGCTCACTGGCAGTGCTTCCATGGCCAAGAAAATGGAC
  ATTATCAAGGGCCTTTTTGTTGCCTGCCGCTACTCAGAAGCCCGGTTTATTGCCAGGTCCCTAAGTGGGC
  GGCTGCGCCTTGGGCTGGCTGAGCAGTCAGTACTGGCTGCTCTTGCCCAGGCTGTGAGCCTCACACCCCC
  TGGCCAAGAATTTCCCACAGCCGTGGTGGATGCTGGGAAGGGCAAGACAGCAGAGGCCAGAAAGATGTGG
  TTGGAAGAACAAGGCATGATCTTGAAGCAAACCTTCTGTGAGGTGCCTGATCTGGATCGCATCATCCCTG
  TGCTGCTGGAACATGGCTTGGAAAGTCTCCCAGAGCACTGCAAGCTGAGCCCAGGGGTTCCTCTTAAACC
  AATGCTGGCCCATCCCACTCGGGGTGTCCGTGAGGTCCTGAAACGCTTTGAGGAGGTCGACTTCACCTGC
  GAGTATAAATACGATGGGCAGCGGGCACAGATACATGTTCTGGAAGGTGGAGAAGTGAAGATCTTCAGCA
  GGAACCAGGAAGACAACTCAGGAAAGTATCCTGACATCATCAGCCGCATCCCCAAGATTAAACACCCCTC
  AGTCACATCTTTCATTCTGGACACTGAGGCTGTGGCCTGGGACCGGGAAAAGAAGCAGATCCAGCCATTC
  CAAGTGCTCACCACACGCAAGCGCAAGGAGGTTGATGCATCGGAGATACAGGTACAAGTGTGTCTGTATG
  CTTTTGACCTCATCTACCTCAACGGAGAGTCCCTGGCTCGCCAGCCCCTGTCTCGACGCCGGCAGCTACT
  CCGTGAGAACTTTGTGGAGACAGAGGGTGAGTTTGTCTTTGCCACCTCCTTGGACACCAAGGACATCGAG
  CAGATTGCCGAGTTCTTAGAGCAGTCAGTGAAGGACTCCTGTGAGGGCCTGATGGTGAAGACCCTGGATG
  TTGATGCTACCTATGAGATCGCCAAGAGGTCACACAACTGGCTCAAGCTGAAGAAGGACTACCTTGAAGG
  TGTGGGCGACACTCTAGACCTTGTGGTGATTGGTGCCTACCTGGGCCGGGGGAAGCGGGCTGGCCGGTAT
  GGGGGCTTCCTGTTGGCTGCCTACGATGAGGAGAGTGAAGAGCTGCAGGCCATATGCAAGCTGGGAACCG
  GATTCAGCGACGAGGAGCTGGAAGAACATCATCAGAGCATGCAGGCCCTTTTATTGCCTACCCCACGCCC
  CTATGTGAGGATTGATGGGGCAGTGGCCCCAGACCACTGGCTAGACCCGAGTGTTGTGTGGGAGGTGAAA
  TGTGCTGATCTCTCCCTGTCTCCCATCTACCCTGCTGCTCGGGGCCTGGTGGACAAAGAAAAAGGGATCT
  CCCTTCGTTTCCCTCGGTTCATTCGTGTCCGTGAGGATAAGCAGCCAGAGCAGGCCACTACCAGTGACCA
  GGTGGCCTCTTTGTACCGGAAGCAGAGTCAGATTCAGAACCAGCAAAGCTCAGACTTGGACTCTGATGTT
  GAAGATTACTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001024268
• Insert Size: 2742 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001024268.2, NP_001019439.1
• RefSeq Size: 3069 bp
• RefSeq ORF: 2742 bp
• Locus ID: 81513
• Cytogenetics: 1q21
• Gene Summary: catalyzes the joining of blunt ended DNA [RGD, Feb 2006]