Cast (NM_001033715) Rat Untagged Clone

CAT#: RN200417

Cast (untagged ORF) - Rat calpastatin (Cast), transcript variant 2, (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Cast
• Synonyms: MGC108997
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN200417 representing NM_001033715
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCCGGCCCGGCCCGAAGCCCGCCGCCTCTTCCAGGCCCCGGCGCGGGGCGGCAGCTAGCCACACCC
  AGGAGCATGTTAATGAAAAGAATATTGGATCATCTTCTAAACCAGCAGAAAAGAAAGGATCTGATGAGGT
  GACAGCTTCCTCTGCTGCCACTGGCACATCTCCCAGAATGAGTACCACAGGAGCTAAGGCTGTGAAAATA
  GAGTCTGAGAAGTCACAATCAAGTGAGCCACCTGTGATTCATGAGAAAAAGCCAAAAGGAAAGCCAAAGG
  AAGGGTCAGAGACAAAATTTCAAGATGCTCCTTCTGCTGATGGAGAGAGTGTGGCTGGAGGTGTGACTGT
  GGCCACTGCCTCAGACAAGGTGGTTGTTAAGAAAAAAGAGAAGAAATCATTAACACCTACTTTACCTATG
  GAGTCCACACTAAACAAGCTATCTGACAAGTCAGGCGTGAATGCTGCTTTGGATGACCTGATAGACACCT
  TAGGTGAATGTGAAGATACTAACAAAGATGATCCACCATATACTGGACCAGTAGTTCTGGACCCAATGGA
  TTCTACCTACCTTGAGGCACTGGGTATAAAAGAAGGGACTATTCCTCCAGAGTATAGGAAACTTCTGGAG
  AAAAATGAAGCTATCACAGGACCTCTTCCAGATTCTCCTAAACCTATGGGAATCGACCATGCTATAGATG
  CCTTGTCATCTGATTTCACCTGTAGCTCTCCAACTGGAAAGCAAACTGAGAAAGAGAAGTCTACTGGGGA
  AAGTTCGAAAGCTCAGTCTGCAGGAGTGACCAGAAGTGCTGTTCCTCCCCAGGAGAAGAAAAGGAAAGTA
  GAAGAGGAGGTCATGAATGATCAAGCCCTGCAGGCTCTGTCAGATTCACTGGGCACCCGGCAGCCAGACC
  CTCAGAGCCATCTTAGGCAAGCCAAACAAGTCAAAGAGGCAAAAGCAAAAGAAGAAAGGCAGGAGAAATG
  TGGTGAAGATGAGGACACAGTACCTGCTGAGTACAGGTTAAAACCAGCCAAGGATAAAGATGGAAAACCA
  CTATTGCCAGAACCTGAAGAAACATCTAAGTGCCTGAGTGAGTCAGAGCTGATTGGGGAGCTTTCAGCAG
  ATTTTGTCCAACCTACATATCAAGAAAAACCATCTATGCCAGCTGCAAAAATAAAGAAGGGCGTAGTGCC
  AGATGATGCTGTAGAAACCTTGGCTAGAAGCCTGGGGACAAGGAAAGAAGATCCAGAAGATGAAAAATCT
  CTGGTGGATAAAGTCAAGGAGAAAGCTAAAGAAGAAGATCATGAAAAACTTGGTGAAAAAGAAGAAACAA
  TTCCTCCTGATTATCGACTAGAAATAGTCAAGGATAAGGATGGAAAACCGCTCCTGCCAAAAGAAGCCGA
  GGAACAACTTCCACCCTTAAGCGATGACTTCCTTCTTGATGCATTGTCTCAGGACTTCTCCAGTCCTGCA
  AACATATTGTCTCTTGGTTTTGAAGATGCCAAACTTTCTGCTGCTGTTTCTGAAACAGTTTCCCAGGTGC
  CTGCTCCAAGCAACCACACAGCAGCCCCACCGCCTGGCACTGAGAGGAGAGACAAAGAACTCGATGATGC
  CTTGGATGAACTTTCTGACAGTCTTGGACAAAGACAGCCTGATCCAGATGAGAACAAACCACTAGATGAT
  AAAGTAAAGGAAAAAATCAAAGCAGAGCATAGCGAGAAACTGGGAGAAAGAGACGACACCATCCCCCCTG
  AATATAGGCATCTCCTGGATAATGATGGGAAGGACAAACCAGAGAAGCCACTGACAAAGAACACAGAGAA
  GCCTGGTCAGGACCAGGACCCCATTGATGCCCTCTCAGAAGACTTGGATAGCTGCCCCCCAACTACAGAG
  ACTTCACAGAATACAACAAAGGAGAAGGGTAAGAAGACTTCAAGTTCCAAAGCATCCAAGAATGAAGAGA
  AAACAAAGGATTCCTCCAAGAAGACAGAGGAAGTGCCCAAGCCAAAGGTGGATGAAGATGCAACATAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001033715
• Insert Size: 2028 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001033715.1, NP_001028887.1
• RefSeq Size: 4535 bp
• RefSeq ORF: 2028 bp
• Locus ID: 25403
• Cytogenetics: 2q11
• Gene Summary: inhibitor of calpain; degradation is required for myoblast fusion; exists in multiple alternatively spliced isoforms [RGD, Feb 2006]
  Transcript Variant: This variant (2) lacks an in-frame exon compared to variant 1. The resulting isoform (b) has the same N- and C-termini but is shorter compared to isoform a.