Adarb1 (NM_001111056) Rat Untagged Clone

CAT#: RN200424

Adarb1 (untagged ORF) - Rat adenosine deaminase, RNA-specific, B1 (Adarb1), transcript variant 3, (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Adarb1
• Synonyms: Adar2; Red1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN200424 representing NM_001111056
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCCCCAAGGACAGCAGCACACCCGGGCCTGGCGAGGGTATTCCGCTCTCCAACGGGGGTGGTGGGA
  GCACCAGCAGGAAGCGGCCCCTGGAGGAGGGCAGCAATGGCCACTCCAAGTACCGCCTGAAGAAGCGAAG
  GAAAACGCCAGGGCCCGTTCTACCCAAGAACGCCCTGATGCAGCTGAACGAGATCAAACCGGGCTTACAG
  TACATGCTGCTGTCCCAGACAGGACCCGTGCACGCACCTCTGTTTGTCATGTCTGTGGAGGTAAACGGGC
  AGGTCTTTGAAGGCTCCGGCCCTACAAAGAAGAAGGCAAAGCTGCATGCTGCTGAGAAGGCCCTGCGGTC
  TTTTGTCCAGTTTCCCAACGCCTCTGAGGCCCACCTGGCCATGGGAAGGACCCTCTCCGTGAACACAGAC
  TTCACGTCCGACCAGGCGGACTTCCCCGACACGCTCTTCAATGGCTTTGAGACTCCAGACAAGTCGGAGC
  CACCCTTCTACGTAGGCTCCAATGGGGATGACTCCTTCAGCTCAAGCGGAGACGTTAGCCTGTCAGCCTC
  CCCAGTGCCTGCCAGCCTTACCCAGCCTCCTCTGCCCATCCCACCACCATTCCCACCCCCAAGTGGGAAG
  AACCCCGTGATGATCTTGAATGAGCTGCGCCCAGGGCTGAAGTATGACTTCCTCTCCGAGAGTGGGGAGA
  GCCACGCCAAGAGCTTTGTCATGTCCGTGGTGGTAGATGGCCAGTTCTTTGAGGGCTCAGGGAGAAACAA
  GAAGCTTGCCAAGGCCCGGGCTGCACAGTCTGCCTTGGCTACTGTCTTCAATTTGCACTTGGACCAAACG
  CCATCTCGCCAGCCTGTCCTCAGTGAGGGTCTCCAGTTGCATTTGCCACAGGTATTGGCAGATGCTGTCT
  CACGCCTGGTCCTGGGTAAGTTCAGTGACCTGACAGACAACTTTTCCTCCCCTCACGCACGAAGAAAAGT
  GCTCTCTGGAGTAGTGATGACCACAGGTACAGATGTCAAAGATGCCAAGGTGATAAGTGTTTCGACAGGG
  ACGAAGTGCATCAACGGCGAATACATGAGTGACCGTGGCCTGGCTCTCAATGACTGCCACGCAGAGATAA
  TCTCCCGAAGGTCCCTGCTCAGGTTTCTATACGCACAGCTCGAGCTTTACTTAAATAACAAAGAAGACCA
  GAAAAAGTCCATATTTCAGAAGTCAGAGCGGGGTGGGTTCCGGCTGAAGGATACCGTGCAGTTCCACCTG
  TACATCAGCACCTCACCCTGCGGAGACGCCAGAATATTCTCTCCCCATGAGCCCGTGCTAGAGGGTATGG
  CGCCAGACTCCCACCAGCTGACAGAACCGGCTGATAGACATCCGAATCGCAAAGCAAGGGGACAGCTGCG
  GACTAAAATAGAATCTGGCGAGGGGACAATCCCTGTGCGCTCAAATGCCAGCATCCAGACCTGGGATGGG
  GTGCTGCAGGGGGAACGGCTGCTCACCATGTCCTGCAGTGACAAGATAGCACGCTGGAACGTGGTGGGCA
  TCCAGGGGGCCCTGCTCAGCATTTTCGTGGAGCCCATCTACTTCTCCAGCATCATCCTGGGCAGCCTGTA
  CCACGGGGACCACCTCTCCAGGGCCATGTACCAGCGGATCTCCAACATAGAGGACCTGCCACCGCTCTAC
  ACCCTCAACAAGCCCCTGCTCAGCGGTATCAGCAATGCAGAGGCACGGCAGCCAGGGAAGGCACCCAACT
  TCAGTGTCAACTGGACGGTGGGCGACACGGCCATTGAGGTCATCAATGCCACAACAGGGAAGGATGAGCT
  AGGCCGCCCCTCCCGCCTGTGTAAGCACGCGCTGTACTGTCGCTGGATGCGGGTACACGGAAAGGTGCCC
  CCCCACCTGCTGCGCACCAAGATCACCAAGCCCACCACCTACCACGAGTCCAAGCTGGCAGCGAAGGAGT
  ACCAGGCTGCCAAGGCACGTCTGTTCACTGCCTTCATCAAGGCGGGGCTGGGCGCCTGGGTGGAGAAGCC
  CACAGAGCAGGACCAGTTCTCCTTCACTCCCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001111056
• ORF Size: 2064 bp
• Insert Size: 2064
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).

Reference Data

• RefSeq: NM_001111056.1, NP_001104526.1
• RefSeq Size: 6459
• RefSeq ORF: 2064
• Locus ID: 25367
• Gene Summary: This gene encodes a double-stranded-RNA-specific adenosine deaminase that is involved in editing pre-mRNAs by site-specific conversion of adenosine (A) to inosine (I). Substrates for this enzyme include ionotropic glutamate receptors (GluR2-6) and serotonin receptor (5HT2C). Studies in rodents have shown that this protein can modify its own pre-mRNA by A->I editing to create a novel acceptor splice site, alternative splicing to which results in down regulation of its protein expression. Additional splicing events result in transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (3, also referred to as 2f) results from an auto RNA editing event (AA->AI) in the intronic sequence that creates a novel acceptor splice site, splicing to which inserts 47 nt into the coding region causing a frame-shift, compared to transcript variant 1. Translation initiation from the upstream AUG will result in a very short protein of 82 aa. However, studies reported in PMID:10331393 show that leaky translation initiation from an in-frame, downstream AUG allows some protein expression, and that this isoform (3) exhibits RNA editing activity. Isoform 3 is 24 aa shorter at the N-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of the transcript and the encoded protein are supported by transcript alignment and PMID:10331393.