Gnas (NM_001024823) Rat Untagged Clone
CAT#: RN200464
Gnas (untagged ORF) - Rat GNAS complex locus (Gnas), transcript variant 2, (10 ug)
"NM_001024823" in other vectors (3)
Product Images
![](https://cdn.origene.com/img/defaults-img-expression-plasmids.jpg)
Specifications
Product Data | |
Type | Rat Untagged Clone |
Tag | Tag Free |
Symbol | Gnas |
Synonyms | ALEX; Gnas1; Gnpas; Nesp55; SCG6 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RN200464 representing NM_001024823
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCCCATCCCCGACGAGACTGACAGTGAGGTCTGTGGACCCCCTGAAGACTCCAAATCTGACATCCA GAGCCCCAGTCAGGCCTTCGAGGAAGTCCAAGTGGGCGGAGACTACAGCCCACCTCCAGAGGAAGCCATG CCATTCGAGATCCAACAGCCCAGCCTGGGAGATTTCTGGCCCACCCTGGAGCAGCCTGGACCATCTGGGA CCCCATCAGGCATCAAAGCCTTCAACCCAGCGATTTTGGAGCCCGGGACCCCCACTGGTGCACACCCAGG CCTGGGAGCCTATAGCCCCCCACCAGAAGAAGCTATGCCATTTGAGTTCAATGAGCCTGCCCAGGAAGAC CGTTGCCAGCCTCCCTTGCAAGTCCCAGACCTTGCGCCAGGGGGTCCGGAAGCATGGGTCTCCAGAGCTC TTCCCGCGGAGCCCGGGAACCTCGGATTTGAAAACACTGGCTTCCGAGAAGACTACAGCCCTCCACCTGA AGAATCTGTGCCATTTCAGCTCGATGGAGAAGAATTCGGGGGCGATAGCCCACCCCCAGGACTCCCGCGA GTCACGCCACAAATCGGCATTGGCGGCGAGTTCCCGACAGTCGCGGTCCCGAGTACGCTCTGCCTCGCTC CCGCCGCGAACGCGCCTCCCCTCTGGGTCCAGGGCGCCATTGGCAGACCATTCCGCGAGGCTGTCAGATC TCCTAACTTCGCATACGACATTTCCCCAATGGAGATCACCAGACCCCTGCTTGAGATTGGCAGAGCCTCC ACTGGGGTCGACGACGACACCGCTGTCAATATGGACAGCCCCCCAATCGCAAGTGATGGCCCGCCCATCG AAGTCTCGGGAGCCCCAGTTAAGAGCGAGCACGCAAAGAGACCCCCACTTGAGCGACAAGCAGCCGAGAC AGGAAACAGCCCTATCAGCAGCACCACTGCAGAGGAGGCAAAAGTCCCCTCCCTCGAGCGAGGAGAAGGA TCCCCCACCCAACCTGAAACAGTGCATATCAAGCCAGCTCCTGTTGCGGAGTCCGGAACGGACTCTTCCA AAGCCGATCCGGACTCAGCTACACACGCAGTTCTTCAGATCGGTCCTGAGGAAGTCGGAGGAGTCCCAAC CATGCCCACCGATCTTCCGCCTGCTTCTGAAGATGCGGGCCCAGATGTCCGGGCAGAACCAGACGGAGGG ACAGCCCCAGCCACCCCTGCCGAGTCCGAAGACAACAGAGAACCAGCCGCCGCCGCCGCCGCCGAGCCAG CCGCCGAGCCAGCCGCTGAGCCAGCCGCCGAGCCAGCCGCCGAGCCAGCCGCCGAGCCAGCTGCCGAGGC AGTCCCTGACACCGAAGCCGAGTCTGCCTCCGGGGCAGTCCCCGACACCCAAGAGGAGCCCGCAGCCGCG GCAGCCTCTGCCACGCCGGCGGAGCCTGCCGCCCGGGCAGCCCCCGTCACCCCTACGGAGCCCGCTACCC GGGCTGTCCCTTCTGCCAGAGCCCATCCAGCCGCCGGGGCTGTCCCTGGAGCCTCAGCGATGTCAGCCGC TGCTAGGGCAGCCGCCGCTAGAGCAGCCTATGCAGGTCCTCTGGTCTGGGGAGCCAGGTCACTCTCGGCT ACTCCAGCCGCTCGGGCATCCCTCCCTGCCCGCGCAGCAGCTGCCGCCCGAGCAGCCTCTGCTGCCCGCG CAGTCGCTGCCGGCCGGTCAGCCTCTGCCGCCCCAAGCAGGGCCCATCTTAGACCCCCCAGCCCGGAGAT CCAGGTTGCTGACCCGCCTACTCCGCGGCCTGCTCCGCGGCCGAGTGCCTGGCCTGACAAATACGAACGT GGCCGAAGCTGCTGCAGGTATGAGGCTGCGTCCGGCATCTGCGAGATCGAGTCCTCCAGCGATGAGTCGG AAGAAGGGGCCACTGGCTGCTTCCAGTGGCTTCTGCGGCGAAACCGCCGCCCTGGCCAGCCCCGGAGCCA CACAGTCGGGAGCAACCCGGTCCGCAACTTCTTCGCCCGAGCCTTCGGAAGCTGCTTCGGTCTATCTGAG TGTACCCGATCACGATCCCTCAGCCCCGGGAAGGCCAAGGATCCTATGGAAGAGAGGCGCAAACAGATGC GCAAAGAAGCCATGGAGATGCGAGAGCAGAAGCGCGCAGATAAGAAACGCAGCAAGCTCATCGACAAGCA ACTGGAGGAGGAGAAGATGGACTACATGTGTACACACCGCCTGCTGCTTCTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
Restriction Sites | SgfI-MluI |
ACCN | NM_001024823 |
Insert Size | 2223 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001024823.4, NP_001019994.4 |
RefSeq Size | 3826 bp |
RefSeq ORF | 2223 bp |
Locus ID | 24896 |
Cytogenetics | 3q43 |
Gene Summary | This locus has a highly complex imprinted expression pattern. It gives rise to maternally, paternally, and biallelically expressed transcripts that are derived from four alternative promoters and 5' exons. Some transcripts contain a differentially methylated region (DMR) at their 5' exons, and this DMR is commonly found in imprinted genes and correlates with transcript expression. In addition, one of the transcripts contains a second overlapping ORF, which encodes a structurally unrelated protein - Alex. Alternative splicing of downstream exons is also observed, which results in different forms of the stimulatory G-protein alpha subunit, a key element of the classical signal transduction pathway linking receptor-ligand interactions with the activation of adenylyl cyclase and a variety of cellular reponses. Multiple transcript variants have been found for this gene. [provided by RefSeq, Apr 2009] Transcript Variant: This variant (1, also known as GNASXL) is paternally expressed. It includes an alternate 5' exon, as compared to variant 3. This variant includes two overlapping open reading frames encoding XLas and Alex, respectively. This RefSeq represents Alex, also known as alexX, which has no similarity to other isoforms encoded by this gene. It interacts with XLas, and their interaction is essential for G-protein signaling in neuroendocrine cells. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RR200464 | Gnas (Myc-DDK-tagged ORF) - Rat GNAS complex locus (Gnas), transcript variant 2, (10 ug) |
USD 420.00 |
|
RR200464L3 | Lenti ORF clone of Gnas (Myc-DDK-tagged ORF) - Rat GNAS complex locus (Gnas), transcript variant 2, (10 ug) |
USD 640.00 |
|
RR200464L4 | Lenti ORF clone of Gnas (mGFP-tagged ORF) - Rat GNAS complex locus (Gnas), transcript variant 2, (10 ug) |
USD 700.00 |
{0} Product Review(s)
Be the first one to submit a review