Btd (NM_001012047) Rat Untagged Clone

CAT#: RN201713

Btd (untagged ORF) - Rat biotinidase (Btd), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Btd
• Synonyms: MGC109548
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN201713 representing NM_001012047
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTGGAGCCCGCACTGCGCATGCGCTCGTCTTCCTCCTCGGCTGTTCTGCGCTTGCGTTGGGCGTCT
  GCTCAGCCTCCCTGGAGCACGGCGAGGCTGAGTACTACGTGGCTGCCGTGTACGAACACCGATCGGTCCT
  GAGCCCAAACCCCCTGGAGCTCAGCAGCCGCCAGCAGGCCCTGGAGCTCATGAAGCAGAACCTCGACGTC
  TATGAACAGCAAGTGATGGCTGCAGCCCAGAAGGGTGCGCACATTATAGTGTTCCCAGAAGACGGTATCC
  ACGGATTCAACTTCACAAGAACATCCATTTACCCATTCTTGGACTTGATGCCATCTCCCAGGCTGGTCAG
  TTGGAACCCGTGTCTGGAGCCCTTTCGCTTCAATGACACGGAGGTCCTCCAGCGCCTGAGTTGCATGGCC
  ATCAAGGGAAAGATGTTCTTGGTGGCCAATCTTGGGACAAAACAGCCTTGCCTCGGCAGCGACCCTGGGT
  GCCCGCAGGACGGGAGATACCAGTTTAACACAAACGTCGCGTTCAGTGACAACGGAACCCTCGTCGGCCG
  CTACCGTAAGCACAACCTGTACTTTGAGGAAGCCTTCGATAGCCCTGCTGACGTGGACCTCACCACCTTT
  GATACGCCGTTCGCGGGCAAGTTCGGTATGTTCACTTGCTTTGATATCCTGTTCTTTGACCCCGCGGTCC
  GGCTCCTCAGAGACTTTGAGGTAAAGCACATTGCGTACCCTACGGCCTGGATGAACCAGCTGCCGCTCTT
  GGCAGCCATTGAAATCCAGAAAGCATTCGCCACCGCCTTCGGCGTCACCGTTCTGGCAGCTAACATCCAC
  CACCCGACTCTGGGGATGACGGGCAGTGGCATACACACCCCTCTGAAGTCCTTTTGGTACCATAACATGG
  ACGACCCCGAAGGTCACCTTATAATTGCCCGGGTAGCCACAAACCCACAGGGCCTCGTTGGGACAGAGAA
  TACAACTAGTGAAATGGATCCGTCCCATAGGAAGTTCTTAAAAGTCTTGTCTGGGGATCCGTACTGTGAG
  AAGGATGCCCAGGAAGTGCGCTGCGACGAGGCTGCCAAGTGGAACCTGAACGCACCACCTACCTTCCACT
  CGGAGATGATGTATGACAATTTCACCCTGGTCCCCGTCTGGGGAAAGGAAGGCCACCTCCAGGTGTGCTC
  CAACAGTCTCTGTTGCCACTTACTTTTCGAGAGGCCCGCCCTGTCCAAAGAGCTATATGCCCTGGGTGTC
  TTTGACGGACTCCACACAGTGCACGGTACCTACTACGTTCAAGCCTGTGCCCTGGTCAAGTGCGGGGGCG
  CTGGCTTTGAAACCTGCGGGCAGGAGATCTCGGAGGCCGAGGGCTTGTTTGACTTTCATCTGTGGGGGAA
  CTTCAGCACCTTGTATGTCTTCCCTTTATTTCTCACCTCGGGGATGACCCTAGACACCCCTGACCAGCTC
  GGGTGGGAGAGCGACCACTATTTCTTGAGGAAGAGGGGACTGTCCTCCGGCCTGGTGACGGCAGCTCTCT
  ATGGGCGGTTGTATGAGAGGAATTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001012047
• Insert Size: 1566 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001012047.1, NP_001012047.1
• RefSeq Size: 1999 bp
• RefSeq ORF: 1566 bp
• Locus ID: 306262
• Cytogenetics: 16p16