Sephs2 (NM_001079889) Rat Untagged Clone

CAT#: RN209287

Sephs2 (untagged ORF) - Rat selenophosphate synthetase 2 (Sephs2), (Note, selenocysteine protein, internal stop codon, see reference data summary)

Specifications

Product Data

• Type: Rat Untagged Clone
• Symbol: Sephs2
• Synonyms: MGC156840
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN209287 representing NM_001079889
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGAAGCGGCGGCAGGCGCCAGCGGAGAAGCCATGGCGGCACTAGTGGCCGCGGAAGGTTCCTTGG
  GCCCGGCGGGCTGGTCTGCTGGCCGGAGTTTCTCCAACTACCGGCCGTTCGAGCCCCAGACACTGGGCTT
  CAGCCCGAGCTGGCGGCTGACGAGCTTCTCCGGCATGAAAGGCTGAGGCTGCAAGGTCCCCCAGGAGACC
  CTGCTCAAACTCCTGGAGGGACTGACGCGGCCCGCGCTGCAGCCCCCGCTTACCTCGGGTCTGGTCGGGG
  GCCAGGAAGAGACGGTGCAGGAAGGGGGCCTGACCACCAGGCCCGGCCCGGGCTCAGCCTTCCCCTCGCT
  GAGCATTGGGATGGACTCCTGCGTCATCCCCCTGAGGCACGGAGGCCTGTCGCTGGTGCAGACCACCGAC
  TTCTTTTACCCCTTGGTGGAAGATCCCTATATGATGGGGCGCATAGCTTGTGCCAATGTGCTCAGTGACC
  TCTATGCCATGGGCATTACCGAGTGTGACAACATGTTGATGTTACTCAGTGTGAGCCAGAGCATGAGTGA
  AAAGGAACGAGAGAAGGTAACGCCGCTCATGATCAAAGGCTTTCGTGACGCTGCGGAAGAGGGAGGAACT
  GCAGTGACTGGTGGACAGACAGTGGTCAACCCTTGGATTATCATCGGTGGGGTTGCCACTGTGGTGTGTC
  AGCAAAATGAATTCATAATGCCTGACAGCGCTGTGGTAGGAGATGTGCTGGTATTAACCAAGCCTTTAGG
  AACCCAGGTTGCTGCCAATGCCCACCAATGGCTGGATAATCCTGAGAAATGGAATAAAATCAAGATGGTG
  GTTTCCAGAGAGGAAGTAGAGCTAGCCTATCAGGAAGCTATGTTCAACATGGCTACTCTGAACAGGACTG
  CTGCTAGCTTGATGCACACTTTTAATGCTCACGCAGCCACGGATATCACAGGCTTTGGCATATTAGGACA
  CTCCCAGAACCTCGCAAAACAGCAAAAAAATGAAGTGTCCTTTGTCATTCACAATCTGCCAATCATTGCC
  AAGATGGCTGCGATCAGCAAAGCCAGTGGGCGCTTTGGCCTCCTCCAAGGAACATCAGCTGAAACCTCTG
  GGGGATTACTGATCTGTCTGCCAAGAGAACAGGCGGCCCGCTTTTGTTCGGAAATCAAATCTTCCAAGTA
  CGGAGAGGGTCACCAAGCTTGGATTGTTGGCATCGTGGAGAAGGGAAACCGGACAGCCCGGATCATTGAC
  AAGCCTCGCGTTATTGAAGTTCTACCTCGGGGAGCCTCTGCTTCTGCTGCTGCTGCTCCTGACAATTCCA
  ATGCAGCCTCTGAGCCTAGTTCTTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001079889
• ORF Size: 1356 bp
• Insert Size: 1356
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). The expression of this clone is not guaranteed due to the nature of selenoproteins.

Reference Data

• RefSeq: NM_001079889.2, NP_001073358.2
• RefSeq Size: 2342
• RefSeq ORF: 1356
• Locus ID: 308993
• Gene Summary: This gene encodes an enzyme that catalyzes the production of monoselenophosphate (MSP) from selenide and ATP. MSP is the selenium donor required for synthesis of selenocysteine (Sec), which is co-translationally incorporated into selenoproteins at in-frame UGA codons that normally signal translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, the Sec insertion sequence (SECIS) element, which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. This protein is itself a selenoprotein containing a Sec residue at its active site, suggesting the existence of an autoregulatory mechanism. It is preferentially expressed in tissues implicated in the synthesis of selenoproteins and in sites of blood cell development. [provided by RefSeq, May 2017]