Grtp1 (NM_001031813) Rat Untagged Clone

CAT#: RN216257

Grtp1 (untagged) - Rat growth hormone regulated TBC protein 1 (Grtp1), transcript variant 2

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Grtp1
• Synonyms: LRRGT00052
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN216257 representing NM_001031813
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATCCCGCAGAGCGCGCGCAGGCGGCGCGAGCTCGGGTACCCAGGATCGATCCGTATGGATTTGAAA
  GGCCCGAAGACTTTGATTATGCGGCTTATGAAGAATTCTTTTCCACGTACCTGGTGATACTCACTAAGAG
  GGCGATCAAATGGTCCAAACTTCTAAAGGGAAGCGGTGGTGTCCGAAAAAGCGTGACAGACTTGAACCGG
  ACATTCCCTGACAACGTGATGTTCCGGAAGACGGCAGATCCTTGTCTCCAGAAGACCCTGTACAACGTGC
  TGCTGGCGTATGGGCTGCACAACCAAGATGTGGGGTACTGCCAGGGGATGAACTTCATAGCTGGGTATCT
  GATCCTTATCACTAAGAATGAAGAGGAGTCTTTCTGGCTTTTGGATGCTCTTGTTGGAAGAATACTGCCT
  GATTACTATAGCCCGGCAATGTTGGGGCTGAAGACTGATCAAGAGGTCCTGGCAGAGCTGGTGAGGATGA
  AGCTACCGGCGGTGGCAGCACTGATGGATGGCCATGGTGTGTTGTGGACTCTGCTGGTGTCCCGCTGGTT
  CATCTGCCTGTTTGTGGATATTCTCCCTGTGGAGACGGTGCTACGAATCTGGGACTGTTTGTTCAACGAA
  GGCTCCAAAATCATATTCCGGGTTGCTCTGACCTTAATTAAGCAACATCAGGAATTTATATTGGAAGCCA
  GCAGTGTTCCAGACATCTGTGACAAGTTCAAGCAGATCACCAAAGGGGACTTTGTGACAGAGTGTCACAC
  GTTCATGCAGAAAATATTTTCAGAACCAGGAAGCCTGTCCATGGCGACCATCACTAGGCTCCGGGAAAGT
  TGCCGAGCTGCGCTGCAGGCACAGAGCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001031813
• Insert Size: 870 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This clone expresses the complete ORF with c-terminal tags of Myc-DDK.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001031813.2, NP_001026983.1
• RefSeq Size: 1111 bp
• RefSeq ORF: 870 bp
• Locus ID: 361180
• Cytogenetics: 16q12.5
• Gene Summary: May act as a GTPase-activating protein for Rab family protein(s). [UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) lacks an in-frame exon in the CDS, as compared to variant 1. The resulting isoform (2) lacks an internal segment, as compared to isoform 1.