Sphk1 (NM_001270810) Rat Untagged Clone

CAT#: RN216533

Sphk1 (untagged) - Rat sphingosine kinase 1 (Sphk1), transcript variant 5

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Sphk1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN216533 representing NM_001270810
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAACCAGCAGACTGTCCCCGGGGACTGCTCCCACGGCCATGCAGAGTGTTGGTGCTGCTGAACCCCC
  GGGGTGGCAAGGGCAAGGCTCTGAAGCTCTTTCAGAGCCGTGTGCGGCCCCTTCTGGAGGAGGCTGAGGT
  ATCCTTTAAACTGATGCTCACCGAACGGCAGAACCATGCCAGGGAGCTGGTGTGTGCAGAAGAACTGGGT
  CACTGGGATGCCTTGGCGGTCATGTCCGGTGACGGTCTGATGCACGAGGTGGTGAATGGGCTAATGGAAC
  GACCTGACTGGGAGAGTGCCATCCAGAAACCCCTATGTAGCCTCCCTGGAGGCTCTGGCAATGCGCTGGC
  AGCTTCTTTGAACTACTATGCTGGGCACGAGCAGGTCACTAATGAAGATCTCCTGATCAACTGCACACTG
  CTGTTATGCTGCCGGCAGCTGTCACCCATGAATCTGCTGTCCCTGCACACCGCTTCCGGACGGCAACTCT
  ATTCTGTTCTCAGTCTGTCCTGGGGTTTCGTTGCTGACGTGGACTTGGAGAGTGAGAAATACAGGAGCTT
  GGGGGAGATTCGTTTCACGGTGGGAACCTTTTTTCGCCTAGCAAGCCTGCGCATTTACCAAGGCCAACTG
  GCCTACCTTCCTGTAGGGAAGGCTGCCTCTAAGATACCTGCTTCCTCACTGGCACAGAAGGGCCCTGCCA
  ACACATACCTCGTTCCTCTGGAGGAGCCAGTGCCACCTCATTGGACTGTGGTGCCAGAGCAGGACTTCGT
  TCTGGTGCTGGTGCTGCTTCACACCCACCTGAGCACAGAGATGTTTGCAGCACCCATGGGCCGCTGCGAG
  GCTGGTGTTATGCATCTGTTCTATATACGTGCGGGGGTGTCAAGGGCCATGCTGCTGCGCCTCTTCCTGG
  CCATGCAGAAGGGCAAGCATATGGACCTCGACTGCCCGTACTTGGTTCATGTGCCAGTGGTTGCCTTCCG
  CCTGGAACCCAGGAACCAGAGGGGAGTGTTTTCTGTGGACGGGGAGCTGATGGTGTGTGAAGCTGTGCAG
  GGCCAAGTGCACCCAAACTACCTTTGGATGGTCTCTGGTAGCAGTGACTCCCCGTCCGGCCGGGACTCCC
  AGCGGAGGCCACCTCCAGAAGAACCAATATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001270810
• ORF Size: 1152 bp
• Insert Size: 1152
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This clone expresses the complete ORF with c-terminal tags of Myc-DDK.

Reference Data

• RefSeq: NM_001270810.1, NP_001257739.1
• RefSeq Size: 2565
• RefSeq ORF: 1152
• Locus ID: 170897
• Gene Summary: catalyzes the conversion of sphingosine to sphingosine 1 phosphate, which plays a role in cell proliferation and differentiation [RGD, Feb 2006]
  Transcript Variant: This variant (5) uses an alternate splice junction at the 3' end of the first exon compared to variant 1. The resulting isoform (b) is shorter at the N-terminus compared to isoform a. Variants 2, 3, 4, 5, and 6 all encode the same isoform (b). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.