Dclk2 (NM_001195832) Rat Untagged Clone

CAT#: RN217138

Dclk2 (untagged) - Rat doublecortin-like kinase 2 (Dclk2), transcript variant 1

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Dclk2
• Synonyms: CL2; CLICK-II; CLICK2; Dck2; RGD1308384
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN217138 representing NM_001195832
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCAGCACCAGGAGCATTGAGCTGGAACATTTTGAAGAACGGGACAAAAGGCCGCGGCCAGGGTCGC
  GGAGAGGAGCTCCCAGCTCCTCCGGGGGCAGCAGCATCTCTGGCCCCAAGGGCAACGGGCTCATCCCCAG
  CCCGGCGCACAGTGCTCACTGCAGCTTCTACCGCACGCGGACCTTGCAGGCCCTCAGCTCGGAGAAGAAG
  GCCAAGAAGGCGCGGTTCTACCGGAATGGGGACCGCTACTTCAAAGGCCTGGTCTTTGCGATCTCCAGCG
  ACCGTTTCCGTTCCTTCGATGCGCTCCTCATAGAGCTCACCCGCTCGCTGTCTGACAATGTGAACCTGCC
  CCAGGGCGTCCGCACAATCTACACCGTAGACGGCAGCCGGAAGGTCACCAGCCTGGACGAGCTGCTGGAA
  GGTGAAAGTTACGTGTGTGCCTCCAACGAACCATTCCGTAAAGTTGACTACACCAAAAACGTTAACCCGA
  ACTGGTCTGTGAACATCAAGGGTGGTACTACCCGCACCTTGGCCGTGGCCTCGGCCAAGAGTGAGGTGAA
  AGAAAGCAAGGACTTCATCAAACCCAAGCTCGTGACTGTGATTCGAAGTGGGGTGAAGCCTAGAAAGGCC
  GTGCGCATCCTTCTGAATAAAAAGACTGCCCATTCCTTTGAGCAGGTCCTAACGGATATCACCGAAGCCA
  TTAAACTAGACTCCGGCGTGGTGAAGAGGCTGTGCACCCTGGATGGAAAGCAGGTTACTTGTCTGCAAGA
  CTTTTTTGGAGACGATGATGTTTTTATTGCATGTGGACCTGAAAAATATCGTTATGCCCAAGATGACTTT
  GTCCTGGATCATAGTGAGTGCCGTGTCCTGAAGTCATCTTATTCTCGGGCCTCAGCTGCTAAGTATTCTG
  GATCCAGAAGCCCAGGGCTCTCCCGCCGCAGCAAGTCACCAGCTTCAGTAAAGAGGGCTGGCCACTCCAG
  TGCCTATTCTACAGCCAAATCCCCAGTGAATGGAACTCCCAGCAGCCAGCTTTCCACTCCTAAGTCCACC
  AAGTCCTCCAGCTCCTCTCCAACCAGCCCGGGAAGTTTCAGAGGACTGAAGCAGATTTCTGCTCAGGGCA
  GATCTTCTTCCAATGTAAACGGTGGGCCCGAACTTGACCGTTGCATGAGTCCTGAAGGCGTGAATGGAAA
  CAGGTGCTCCGAGTCATTCACTCTTCTGGAGAAATACAGAATCGGGAAGGTCATCGGAGACGGCAACTTT
  GCCGTAGTTAAAGAATGTATGGACAGGTCCACTGGAAAGGAGTTTGCATTAAAGATTATTGACAAAGCCA
  AATGCTGTGGAAAGGAACATCTGATTGAGAACGAAGTGTCAATCCTGCGCCGGGTGAAGCACCCCAACAT
  CATCATGCTGGTTGAGGAGATGGAAACAACAACTGAGCTCTTCCTAGTGATGGAACTGGTCAAAGGTGGA
  GATCTCTTCGACGCGATCACTTCTTCAACCAAGTACACCGAGAGAGATGGCAGTGCCATGGTGTACAACC
  TAGCCAGCGCCCTCAGATACCTGCACGGCCTCAGCATCGTGCACAGAGATATCAAACCTGAGAATCTGCT
  GGTGTGCGAATACCCAGACGGAACCAAGTCGCTGAAGCTGGGAGACTTTGGGCTGGCGACGGTGGTTGAA
  GGCCCGTTGTACACAGTCTGTGGCACCCCAACTTACGTGGCACCCGAGATCATTGCTGAAACAGGTTACG
  GCCTGAAGGTGGATGTTTGGGCAGCTGGTGTGATTACGTACATTCTTCTCTGTGGATTCCCACCGTTCCG
  GAGTGAGAACAACCTCCAGGAGGATCTCTTTGACCAGATCTTGGCCGGAAAGCTGGAGTTTCCAGCTCCC
  TACTGGGACAACATTACAGACTCTGCCAAGGAGTTAATCAGTCAAATGCTTCAGGTAAACGTTGAAGCTC
  GCTGTACTGCGGGAGAAATTCTGAGTCACCCCTGGGTGTCAGATGATGCATCCCAGGAGAATAATATGCA
  AGCCGAGGTTACAGGTAAACTAAAACAGCACTTTAATAATGCGCTCCCCAAACAGAACAGCACCACCACT
  GGGGTCTCCGTTATCATGGTCCAAGGCCATGAACACGGCTCTAGATAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001195832
• ORF Size: 2148 bp
• Insert Size: 2148
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This clone expresses the complete ORF with c-terminal tags of Myc-DDK.

Reference Data

• RefSeq: NM_001195832.1, NP_001182761.1
• RefSeq Size: 3982
• RefSeq ORF: 2148
• Locus ID: 310698
• Gene Summary: This gene encodes a member of the protein kinase superfamily and the doublecortin family. The protein encoded by this gene contains two N-terminal doublecortin domains, which bind microtubules and regulate microtubule polymerization, a C-terminal serine/threonine protein kinase domain, which shows substantial homology to Ca2+/calmoduline-dependent protein kinase, and a serine/proline-rich domain in between the doublecortin and the protein kinase domains, which mediates multiple protein-protein interactions. The microtubule-polymerizing activity of the encoded protein is independent of its protein kinase activity. Mouse studies show that this gene and the DCX gene, another family member, share function in the establishment of hippocampal organization and that their absence results in a severe epileptic phenotype and lethality, as described in human patients with lissencephaly. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Sep 2010]
  Transcript Variant: This variant (1) is an exon-5-containing and exon-17-containing transcript (PMIDs:15611072 and 18075264). It has an additional exon in the 3' region, which results in frame-shift, as compared to variant 2. The resulting isoform (a) has a shorter and different C-terminus, as compared to isoform b. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.