Nfasc (NM_001160314) Rat Untagged Clone

CAT#: RN217559

Nfasc (untagged) - Rat neurofascin (Nfasc), transcript variant 1

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Nfasc
• Synonyms: NF
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN217559 representing NM_001160314
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCAGGCAGCAGGCGCCACCCTGGGTCCACGTAGCCCTCATCCTCTTCCTCCTCAGCCTCGGAGGGG
  CCATTGAGATTCCGATGGATCCAAGCATTCAGAATGAGCTGACCCAACCCCCAACGATCACCAAGCAGTC
  GGTGAAGGACCACATCGTGGACCCCCGAGATAACATCCTGATTGAATGTGAAGCTAAAGGGAACCCCGCC
  CCCAGTTTCCACTGGACTCGCAACAGCAGGTTCTTCAACATTGCCAAGGACCCACGGGTGTCCATGAGGA
  GGAGGTCTGGGACCTTGGTGATCGACTTCCGCAGTGGTGGGCGGCCTGAGGAGTACGAAGGGGAGTACCA
  GTGCTTTGCCCGGAACAAATTCGGCACAGCTCTTAGCAACCGCATCCGCCTGCAGGTGTCCAAATCTCCC
  CTGTGGCCCAAGGAAAACCTAGACCCCGTCGTGGTTCAAGAGGGTGCCCCCTTAACCCTGCAGTGCAACC
  CCCCACCTGGCCTCCCATCCCCCGTCATCTTCTGGATGAGCAGCTCCATGGAGCCCATCACCCAGGACAA
  GCGTGTCTCCCAGGGTCACAACGGGGACCTGTACTTCTCCAACGTCATGCTGCAGGACATGCAGACCGAC
  TACAGCTGCAATGCACGCTTCCACTTCACCCACACCATTCAGCAGAAGAATCCCTTCACCCTCAAAGTCC
  TCACCACCCGAGGAGTTGCGGAAAGAACACCTAGCTTCATGTATCCCCAGGGCACGTCAAGCAGTCAGAT
  GGTACTGCGCGGCATGGACCTGCTGCTGGAGTGCATTGCCTCTGGGGTCCCAACACCAGATATTGCATGG
  TACAAGAAAGGTGGGGACCTCCCATCTGACAAGGCCAAGTTCGAGAATTTTAACAAGGCTCTGCGCATCA
  CCAATGTCTCTGAAGAGGACTCTGGGGAGTATTTCTGCCTGGCCTCCAACAAGATGGGCAGCATCCGGCA
  CACGATCTCGGTGAGAGTAAAGGCTGCCCCATACTGGCTGGATGAGCCCAAGAACTTGATCCTGGCTCCT
  GGTGAAGATGGGAGGCTGGTGTGTCGAGCCAATGGGAACCCGAAGCCGACCGTCCAGTGGCTGGTGAATG
  GAGACCCTTTGCAATCGGCACCACCCAACCCCAACCGTGAGGTGGCCGGAGACACTATCATCTTCCGGGA
  CACTCAGATCAGCAGCAGGGCAGTGTACCAGTGTAACACATCCAACGAACATGGCTACCTGCTGGCCAAT
  GCCTTCGTCAGCGTATTAGATGTACCCCCTCGGATGCTGTCTCCCCGGAACCAGCTCATCAGGGTGATCC
  TTTACAACCGGACGCGACTGGACTGTCCGTTCTTTGGGTCTCCCATTCCAACACTCCGATGGTTTAAGAA
  TGGGCAAGGAAGCAACCTGGATGGTGGTAACTACCACGTCTACGAAAATGGCAGCCTGGAAATCAAGATG
  ATTCGCAAAGAGGACCAAGGCATCTACACCTGTGTGGCCACCAACATCCTGGGAAAAGCTGAAAATCAAG
  TCCGCCTGGAGGTCAAAGACCCCACCAGGATCTACAGGATGCCTGAAGACCAGGTGGCCAAGAGGGGCAC
  CACAGTGCAGCTGGAGTGCCGTGTGAAGCATGACCCCTCCTTGAAACTCACAGTCTCCTGGCTGAAGGAT
  GACGAGCCACTCTACATTGGAAACAGGATGAAAAAGGAAGATGACTCCCTGACCATCTTCGGAGTGGCAG
  AGCGGGACCAAGGCAGTTACACGTGCATGGCCAGCACCGAGCTGGACCAGGACCTGGCAAAGGCCTACCT
  CACTGTTCTAGCTGATCAGGCCACTCCGACTAACCGTTTGGCTGCCCTGCCCAAAGGGCGGCCAGACCGA
  CCCAGGGACCTGGAGCTCACTGACCTGGCGGAAAGGAGTGTGAGGCTGACCTGGATCCCGGGGGATGATA
  ACAACAGTCCCATCACAGACTACGTTGTCCAGTTTGAAGAGGACCAGTTCCAGCCAGGAGTCTGGCATGA
  CCACTCCAAGTTCCCAGGCAGTGTCAACTCAGCCGTCCTCCATCTGTCCCCGTATGTCAACTATCAGTTC
  CGAGTCATCGCTGTCAACGAGGTTGGGAGCAGCCACCCCAGCCTTCCATCCGAGCGGTACCGAACAAGCG
  GGGCACCCCCTGAATCCAACCCCAGTGATGTGAAGGGCGAAGGGACACGAAAAAACAATATGGAGATCAC
  ATGGACGCCTATGAATGCTACCTCCGCCTTTGGCCCCAACCTGCGCTACATTGTCAAGTGGCGACGGAGA
  GAAACCCGAGAGACTTGGAACAATGTCACCGTGTGGGGCTCTCGCTATGTGGTGGGGCAGACCCCTGTCT
  ACGTACCCTATGAGATCCGAGTCCAGGCTGAAAATGACTTTGGGAAAGGCCCGGAGCCTGAAACCGTCAT
  TGGGTACTCGGGGGAAGATTTACCCAGTGCCCCCAGGCGTTTCAGAGTCCGACAGCCCAACCTGGAGACC
  ATCAACCTGGAATGGGATCACCCAGAGCACCCCAACGGGATCCTGATTGGATACACGCTCAGATACGTGC
  CCTTTAATGGAACCAAACTGGGAAAGCAGATGGTGGAAAACTTCTCTCCCAATCAGACCAAGTTCTCGGT
  GCAGAGAGCAGACCCCGTGTCCCGTTACCGCTTCTCCCTCAGTGCCAGGACGCAGGTGGGCTCTGGAGAA
  GCAGCCACGGAGGAGTCCCCAACACCTCCAAATGAAGCTACTCCAACTGCAGCTCCTCCCACATTGCCCC
  CGACTACCGTGGGTACCACAGGCCTTGTGAGCAGTACTGATGCTACTGCCCTTGCTGCCACCAGTGAAGC
  CACAACAGTTCCCATCATCCCAACGGTGGTACCTACCACCGTCGCCACCACCATTGCCACAACTACTACA
  ACCACTGCCGCCGCCACCACCACTACCACCACGGAGAGTCCTCCCACTACCACCACTGGGACTAAGATTC
  ACGAAACCGCCCCCGACGAGCAGTCCATTTGGAACGTCACGGTGCTCCCCAACAGTAAATGGGCCAACAT
  CACCTGGAAGCACAATTTCAGGCCTGGAACTGACTTTGTGGTTGAGTACATCGACAGCAACCATACGAAA
  AAAACTGTCCCTGTTAAGGCCCAAGCCCAGCCCATACAGCTGACAGACCTCTTTCCCGGGATGACGTACA
  CGTTGCGGGTGTATTCCCGGGACAACGAGGGCATCAGCAGTACCGTCATCACCTTTATGACCAGTACAGC
  TTACACCAACAACCAGACTGACATCGCCACCCAGGGCTGGTTCATCGGGCTCATGTGTGCCATTGCCCTT
  CTGGTGCTGATCCTGCTGATCGTCTGCTTCATCAAGAGGAGTCGTGGCGGCAAGTACCCAGTGCGAGAAA
  AGAAGGATGTCCCCTTGGGCCCTGAAGACCCCAAAGAAGAAGATGGTTCATTTGACTACAGTGACGAGGA
  CAACAAGCCCCTGCAGGGCAGCCAGACATCTCTGGACGGCACCATCAAGCAGCAGGAGAGTGACGACAGC
  CTAGTGGACTACGGCGAGGGTGGCGAGGGCCAGTTCAACGAAGATGGCTCCTTTATTGGCCAGTACACTG
  TCAGAAAGGACAAGGAGGAGACCGAGGGCAATGAGAGCTCAGAGGCCACATCTCCAGTCAATGCCATCTA
  TTCCCTGGCCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001160314
• ORF Size: 3723 bp
• Insert Size: 3723
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This clone expresses the complete ORF with c-terminal tags of Myc-DDK.

Reference Data

• RefSeq: NM_001160314.1, NP_001153786.1
• RefSeq Size: 9825
• RefSeq ORF: 3723
• Locus ID: 116690
• Gene Summary: This gene encodes an L1 family immunoglobulin cell adhesion molecule with multiple IGcam and fibronectin domains. The protein functions in neurite outgrowth, neurite fasciculation, and organization of the axon initial segment (AIS) and nodes of Ranvier on axons during early development. Both the AIS and nodes of Ranvier contain high densities of voltage-gated Na+ (Nav) channels which are clustered by interactions with cytoskeletal and scaffolding proteins including this protein, gliomedin, ankyrin 3 (ankyrin-G), and betaIV spectrin. This protein links the AIS extracellular matrix to the intracellular cytoskeleton. This gene undergoes extensive alternative splicing, and the full-length nature of some variants has not been determined. [provided by RefSeq, May 2009]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). This isoform lacks the third fibronectin type 3 (FNIII) repeat and includes the mucin-like domain. Isoform 1 is also known as NF186, as described by Davis et al. (PMID: 8947556). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.