Pola1 (NM_053479) Rat Untagged Clone

CAT#: RN217636

Pola1 (untagged) - Rat polymerase (DNA directed), alpha 1, catalytic subunit (Pola1)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Pola1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN217636 representing NM_053479
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGCCTGTTCACGGAGACGACTGCAAACTGGAAACAAGCGCTGTGTCAGATTCAGGGAGTTTTGTAG
  CTTCTCGAGCACGTCGAGAAAAAAAATCAAAGAAAGGACGCCAAGAAGCTTTAGAGCGACTGAAAAAGGC
  TAAAGCTGGTGAGAAGTACAAGTATGAAGTTGAGGACCTCACAAGTGTTTATGAAGAAGTTGATGAAGAG
  CAGTATTCAAAACTGGTTCAGGCACGTCAGGATGATGACTGGATCGTGGATGACGACGGTATAGGCTATG
  TGGAAGACGGTAGAGAGATTTTTGATGACGACCTTGAAGATGATGCACTTGACACCTGTGGGGAAGGAAG
  TGATGGCAAAGCACACAGAAAGGACAGGAAGGATGTAAAGAAACCTTCAGTGACAAAACCAAACAATATC
  AAGGCAATGTTCATTGCTAGTGCTGGGAAGAAAACCACAGATAAAACAGTTGACTTGTCCAAGGATGATC
  TGCTAGGTGACATTCTACAGGATCTGAACACTGAGACACCTCAAATAGCTCCACCGCCAGTATTGATACC
  AAAGAAAAAAAGATCTACTGGAGCTTCACCGAATCCTTTCTCTGTGCACACCGCCACGGCAGTTCCTTCA
  GGAAAAATTGCTTCCCCTGTCTCCAGAAAGGAGCCTCCATTAACTCCTGTTCCTCTTAAACGTGCTGAAT
  TTGCTGGCGATCTGGCTCAGCCAGAGTGTCCAGAAGATGAGCAGGAGTCAGGGGTTATAGAGTTTGAAGA
  TGGCGACTTTGATGAGCCCATGGACACAGAGGAAGTAGATGAGGAGGAGCCTGTGACTGCCAAGATTTGG
  GACCAAGAGAGTGAGCCAGTGGAAGGGGTGAAGCATGAGGCAGATCCTGAGACGGGAACCACATCTTTCT
  TAGACAGTTTTCTCCCAGATGTTTCTTGTTGGGACATTGATCAGAAGGACGAAAACAGTTTCTTACTTCA
  AGAAGTTCAAGTGGACTCCAATCATCTCCCGTTGGTAAAAGGGGCAGATGATGAACAAGTCTTTCAGTTT
  TATTGGCTGGATGCTTATGAAGATCCATACAACCAACCAGGTGTGGTTTTTCTGTTTGGCAAAGTATGGG
  TCGAATCAGCCAAAACCCATGTGAGCTGTTGTGTTATGGTGAAAAATATTGAGCGAACACTCTACTTCCT
  TCCCCGTGAAATGAAAATTGACCTAAACACAGGGAAAGAAACAGCAACTCCAATTACAATGAAGGATGTT
  TATGAAGAATTTGATTCAAAAATTTCAGCAAAATATAAAATAATGAAGTTCAAGTCCAAGATAGTGGAAA
  AGAACTATGCTTTTGAGATACCTGATGTTCCAGAAAAATCTGAGTACTTGGAAGTTAGATACTCGGCAGA
  AGTGCCACAGCTTCCACAGAATTTAAAAGGAGAAACATTTTCTCATGTATTTGGAACTAACACGTCTAGC
  TTGGAACTGTTCTTGATGAATAGAAAGATCAAAGGACCCTGTTGGCTTGAAGTAAAAAATCCACAACTCT
  TGAATCAACCAATCAGTTGGTGTAAATTTGAGGCAATGGCTTTGAAACCAGACTTGGTGAATGTAATTAA
  GGATGTCAGTCCTCCTCCACTTGTGGTGATGTCTTTCAGCATGAAGACAATGCAAAATGTACAGAACCAT
  CAACACGAGATCATTGCTATGGCAGCGCTGGTTCATCACAATTTCCCATTAGATAAAGCACCTCCAAAAC
  CTCCCTTTCAGACGCATTTCTGTGTTGTGTCTAAACCAAAGGACTGTATTTTCCCATGCGCTTTCAAAGA
  AGTCATTAAGAAAAAGAATATGGAGGTTGAGGTTGCTGCAACAGAAAGAACTTTGCTAGGATTTTTCCTT
  GCAAAAGTTCACAAACTTGACCCTGATATTCTCGTGGGTCATAATATTTGTGGTTTTGAACTAGAAGTGC
  TACTGCAAAGAATTAATGAGTGCAAGGTTCCTTTCTGGTCCAAGATAGGTCGGCTGAGGAGATCCAACAT
  GCCAAAACTTGGGAGCCGGAGTGGATTTGGTGAAAGAAATGCTACCTGTGGTCGAATGATATGTGATGTG
  GAAATTTCAGTAAAAGAATTGATTCATTGTAAAAGTTACCATTTGTCTGAACTTGTTCAGCAGATTCTGA
  AAACTGAGAGGATTGTAATTCCAACAGAAAATATACGAAACATGTATAGTGAACCTTCTCATCTGTTGTA
  CCTGTTGGAACACATCTGGAAAGATGCCAGATTCATTTTGCAGATCATGTGTGAGCTAAATGTTCTTCCA
  TTAGCATTGCAGATCACTAACATCGCTGGGAACATTATGTCTAGGACACTGATGGGTGGACGATCTGAGC
  GAAACGAGTTTTTGTTGCTTCATGCATTTTATGAAAACAACTATATTGTGCCTGACAAGCAGATTTTCAG
  AAAACCTCAGCAAAAACCGGGAGATGAAGATGAAGAAATTGATGGGGATACCAATAAATATAAGAAAGGA
  CGCAAGAAAGCAGCTTATGCTGGAGGCTTGGTTTTGGACCCCAAAGTTGGTTTTTATGACAAGTTCATTT
  TGCTCCTGGATTTCAACAGTTTATATCCTTCCATCATTCAGGAATTTAACATTTGTTTTACAACAGTGCA
  AAGAGTTGCTTCAGAGACACTGAAAGCTACAGAGGATGAGGAACAAGAACAGATTCCAGAGTTGCCAGAT
  CCAAACTTAGATATGGGCATTTTGCCCAGAGAGATCCGGAAACTGGTAGAGCGGAGAAAACAAGTGAAAC
  AGCTAATGAAACAGCAAGATTTAAATCCAGACCTTGTTCTTCAGTATGATATCCGACAGAAGGCTTTGAA
  GCTCACAGCGAACAGTATGTATGGTTGCCTGGGATTTTCGTACAGCAGATTTTATGCTAAACCGCTGGCT
  GCCTTGGTGACATACAAAGGAAGGGAGATTTTGATGCACACGAAAGAGATGGTACAGAAGATGAATCTTG
  AAGTTATTTATGGAGACACAGATTCAATTATGATAAATACCAATAGCACCAACCTGGAAGAAGTCTTTAA
  GTTGGGAAACAAGGTAAAAAATGAAGTGAATAAATTGTACAAACTGCTTGAAATAGACATTGATGGTGTT
  TTCAAGTCTCTGCTATTGCTGAAGAAAAAGAAATATGCTGCTCTAGTTGTCGAACCAACATCCGATGGGA
  ATTACATCACCAAGCAGGAACTGAAAGGATTGGATATAGTTAGAAGGGACTGGTGTGATCTTGCCAAGGA
  CACCGGAAACTTTGTCATTGGCCAGATTCTTTCTGATCAAAGTCGGGACACCATTGTGGAAAACATTCAG
  AAGAGGTTAATAGAAATTGGAGAAAATGTGCTAAACGGCAGTGTCCCAGTGAGCCAGTTTGAAATTAACA
  AGGCATTGACAAAGGATCCCCAGGATTACCCTGATAAAAAAAGTCTACCTCATGTACATGTTGCACTGTG
  GATAAATTCTCAAGGAGGCAGGAAGGTGAAAGCTGGCGATACAGTGTCATATGTTATCTGTCAGGATGGG
  TCAAACCTCCCTGCAACTCAGAGGGCCTATGCACCCGAGCAATTGCAGAAACAGGACAATTTAGCCATCG
  ACACCCAGTACTACTTGGCCCAGCAGATCCACCCTGTTGTGGCTCGAATCTGTGAGCCAATAGATGGAAT
  TGATGCTGTACTCATTGCATTATGGCTAGGACTTGACTCCACTCAATTTAGAGTTCATCAGTATCACAAA
  GATGAAGAGAATGATGCTCTCCTTGGTGGTCCAGCACAGCTAACTGATGAAGAGAAATACAAGGATTGTG
  AAAAATTCAAATGCCTTTGTCCTTCATGTGGAACTGAAAATATTTATGATAATGTCTTTGAAGGCTCGGG
  AATGGATATGGAGCCCAGCTTGAATCGTTGCAGTAATATTGATTGTAAGGCTTCACCAGCGACCTTTATG
  GTACAGTTGAGCAACAAATTGATCATGGACATTAGACGTTGCATTAAAAAGTACTATGATGGCTGGTTGA
  TATGTGAGGAGCCAACTTGTCGCAATCGCATTCGTCGTCTTCCCCTTCATTTCTCCCGAAATGGGCCTCT
  GTGTCCAGCCTGCATGAAAGCTGTGCTCAGACCAGAGTATTCTGACAAGTCCCTGTACACCCAGCTGTGC
  TTTTACCGGTACATTTTTGATGCGGACTGTGCACTGGAAAAACTTCCTGAACACGAGAAAGATAAATTGA
  AGAAGCAGTTTTTCACTCCCAGAGTTCTTCAGGACTACAGAAAAGTCAAGAACATAGCAGAACACTTCTT
  GTCCTGGAGTGGCTACTCTGAAGTAAACCTCAGCAAGCTCTTCGCCAATTATGCTGGGAAGAACTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_053479
• ORF Size: 4407 bp
• Insert Size: 4407
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This clone expresses the complete ORF with c-terminal tags of Myc-DDK.

Reference Data

• RefSeq: NM_053479.1, NP_445931.1
• RefSeq Size: 5321
• RefSeq ORF: 4407
• Locus ID: 85241
• Gene Summary: exhibits DNA polymerase alpha activity; may mediate DNA replication process [RGD, Feb 2006]