Zfp458 (NM_001024299) Rat Tagged ORF Clone

CAT#: RR200021

Zfp458 (Myc-DDK-tagged ORF) - Rat zinc finger protein 458 (Zfp458), transcript variant 2, (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Zfp458
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR200021 representing NM_001024299
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTGGAGAATTACAGCAACCTTGTGTTCCTGGGTCTTGCTTCCTCTAAGCCATACCTGATTACACTTC
  TGGAACAAATAAAAGAGCCTTCAGATATGAAGAAACGAGAGGCTATCACTCTGCACCCAGGGGGAAAATG
  CTATATGTGCGAAGAATGTGGCCAAGTCTTTGAGTGGAAAAAAGTACTTCAGAATCACCAGAAAATTCAT
  TTAAGAGCAAAGCCTTACAAGTGTGAAGAATGTGGCAAGTCTTTCCATTTTCCATCATTACTTTCTGCAC
  ACAAAAGAAATCATACAGGAGAAAAACTCTACAACTGTGAAATATGTTGTAAAGCCTTCCATGCTTCTTC
  ACTGCTTTCTTTACACAAGAGAATTCATACAGGACAGAAACCCCACGAGTCTGAAAATTATAGCAAGGAC
  TTCCATTATCCATCATTACTTTCTCAAAACAAGGTAGTTCATACAGGAGAAAAATCCTACCAATGTGAAG
  ACCATGGCAAGACCTTCCATTATCCATCAACATTTTCCAAGCATAAGAAAATTCATACAGGAGAGAAACC
  ACACAAGTGTGAAGTATGTGGCAAGGCCTTCGACTATCCATCAAGACTTTCCAACCATAAGAGAATTCAT
  ACAGGAGAAAAACCCTACAAATGTGAAGTATGTGACAGAGCCTTCCATGATCCATCAAAACTTTCTCAAC
  ACAAAATAATTCATACAGGAGAAAAACCCTACAAATGTGACGTTTGTGGCAAGGCCTTTCATTATCCATC
  CATACTTTCTAAACATAAGATAATTCATACAGAAGAGAATCTCTGCAAGTGTGATATATGTGGCAAGGCC
  TTCCATTATCCATTATTACTTTCTCAACACAAAATAGTTCATACAGGAGAAAAACCCTACAAATGTGAAG
  ACTGTGGCAAAGCCTTCTATTATCCATCAAGACTTTCCAGACATAAGAAAATACATACAGGAGAGAAACC
  ATACAAGTGTGAAGTATGTGGCAAGGCCTTCCATTATCTATCCATACTTTCTAAACACAAGATAGTTCAT
  ACAGAAGAGAATCCCTACAAGTGTGAAGTATGTGGCAAGGCCTTTGATTATCCATCAAGGCTTTCCAACC
  ATAAAAAAATTCATACAGAAGAGAAACCATACAAGTGTGAAGTATGTGGAAATGCCTTCTGTTTTTCATC
  ATCACTTCGTAAACACAAGATAATTCACACAGGAGAGAAACCCTACAAGTGTGAAGTATGTGGCAAGGCC
  TTTGGTTCTCCATCAAGACTTTCCAAACATAAGAAAATTCATACAGAAGAGAAACCATACAAGTGTGAAG
  TATGTGGAAAGGCCTTCCACTTTCCATCATTACTCTCGGTACACAAGAGAATTCATACGGGAGAAAAACC
  CTACAAGTGTGAGATATGTGGCAAAGCCTTCTATTGTGCATCAACACTTTCTGTACACAAGAGAATTCAT
  ACTGGAGAAAACAACTACAAGTGTGAAGTATGTGGCAAAGCCTTCCATTGTCCATCAACACTTTCAGTGC
  ACAGGAGAATTCATACTCAAGAAAAACCATACAAGTGTGAAGTATGTGGCCAGGCATTCCATGTTTCATC
  TAAACTCTCTTATCATAAGAGAATTCATACAGCAGAAAAACCCTACAAATGTGAAATTTGTGGCAAGGCC
  TTCTATTATCCATCAAGACTTTCCAAGCATAAGATAGTTCATATGGGAGAGAAACGCTACCATGGTCAAG
  CATGTGGCAAGGCCCTTGATTATCCATCAAGACATTCTAAACATAAGAAACTTAATCCAGGAGAGAAACC
  ACACAAATGTGAAATATGTGGAAAAGCCTTCCACTTTCCATCATTACTTTCGATACACAAGAAAATTCAT
  ACTGGAGAAAAAACCTGTAAGTGTGAAGTATGTGGCAAGGACTTCCATTATCCATCAAGACTTTCAAACC
  ACAAGAAAATTCATACAGAAGGGAAAGCATACAAGTGTGATATTTGTGACAAGACCTTCAATTATAAATC
  ATTACTTTCTAAACACAAGATTGGTCATACAGTAGAGAATACCTACCACAGTGAAGTATGTGGGAAGGCC
  TTTGGTTATTCCTCAAGAATTTCCAAAGATAAGAAACTTCATTCAGGAGAGAAACTA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR200021 representing NM_001024299
  Red=Cloning site Green=Tags(s)
  
  MLENYSNLVFLGLASSKPYLITLLEQIKEPSDMKKREAITLHPGGKCYMCEECGQVFEWKKVLQNHQKIH
  LRAKPYKCEECGKSFHFPSLLSAHKRNHTGEKLYNCEICCKAFHASSLLSLHKRIHTGQKPHESENYSKD
  FHYPSLLSQNKVVHTGEKSYQCEDHGKTFHYPSTFSKHKKIHTGEKPHKCEVCGKAFDYPSRLSNHKRIH
  TGEKPYKCEVCDRAFHDPSKLSQHKIIHTGEKPYKCDVCGKAFHYPSILSKHKIIHTEENLCKCDICGKA
  FHYPLLLSQHKIVHTGEKPYKCEDCGKAFYYPSRLSRHKKIHTGEKPYKCEVCGKAFHYLSILSKHKIVH
  TEENPYKCEVCGKAFDYPSRLSNHKKIHTEEKPYKCEVCGNAFCFSSSLRKHKIIHTGEKPYKCEVCGKA
  FGSPSRLSKHKKIHTEEKPYKCEVCGKAFHFPSLLSVHKRIHTGEKPYKCEICGKAFYCASTLSVHKRIH
  TGENNYKCEVCGKAFHCPSTLSVHRRIHTQEKPYKCEVCGQAFHVSSKLSYHKRIHTAEKPYKCEICGKA
  FYYPSRLSKHKIVHMGEKRYHGQACGKALDYPSRHSKHKKLNPGEKPHKCEICGKAFHFPSLLSIHKKIH
  TGEKTCKCEVCGKDFHYPSRLSNHKKIHTEGKAYKCDICDKTFNYKSLLSKHKIGHTVENTYHSEVCGKA
  FGYSSRISKDKKLHSGEKL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001024299
• ORF Size: 2157 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001024299.1, NP_001019470.1
• RefSeq Size: 3293 bp
• RefSeq ORF: 2160 bp
• Locus ID: 499563
• Cytogenetics: 2q22
• MW: 82.8 kDa