Tbc1d14 (NM_001034021) Rat Tagged ORF Clone

CAT#: RR200076

Tbc1d14 (Myc-DDK-tagged ORF) - Rat TBC1 domain family, member 14 (Tbc1d14), transcript variant 2, (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Tbc1d14
• Synonyms: SRF-2; UR-NR#2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR200076 representing NM_001034021
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACTGATGGAAACCTTTCCACCTCTATGAATGGTGTAGCATTGATGGGCATTCTAGATGGTCGGCCAG
  GAGACTCCCTTCAGGAATTGCAACACCTTAGTATCAAGGCAGTGCCCAGATCCCTTTCAGTGCCTGACTA
  TGGACCTACACTAAAACTTGGTGCTTTGGAAGATCGACACAGCCTTCAGTCAGTGGACTCGGGCATTCCT
  ACCCTGGAGATTGGCAACCCAGAGCCTGTTCCCTGCAGTGTGGTCCATGTGAAGAGAAAGCAGTCTGAGT
  CAGAGATCATCCCGGAGCGGGCCTTCCAGAGTGCGTGCCCCTTGCCATCCTGTACACCCTCAGCTCCCAC
  TGGCAGCGAACGGGAGCAAGCTGTGCGGAAGTCTTCCACCTTTCCCAGGACAGGCTATGACTCAGTGAAA
  CTCTACAGCCCCACCTCCAAAGCCTTGAGCCGAAGTGACGATGTCTCTGTCTGCAGTGTGTCTAGTCTTG
  GCACAGAACTGTCAACCACATTGTCGGTTAGCAATGAGGACATCTTGGACCTCATGGTCACGAGCAATTC
  CAGTGCCATTGTGACGCTGGAGAACGATGATGACCCCCAGTTTACTGATGTCACCCTGAGCTCCATCAAG
  GAAACCAGTGACTTACACCAGCAGGATTGTGTTGCTGAGACTGAGGAGGGGAGGAAATTGAGGCTATTGC
  AACCATTCAGCCACTTCTTCACAAGGAATTTGCTTGCTAGAAAGCAAAATGCAAGACTTGATAGACAGAG
  AGATTTGGGATGGAAGCTGTTTGGAAAAGTCCCACTCCGAGAGACTGCTCAGAAAGATTCAAAGAAAACA
  CAAAAGGAATATGAAGACAAGGCTGGAAGACCAAGCAGGCCGCCCTCTCCAAAGCAGAATGTGAGGAAGA
  ACCTGGATTTTGAGCCACTGTCCACCACAGCGCTCATCCTTGAGGACAGACCCGCGAATCTCCCAGCAAA
  GCCAGCTGAAGAAGCTCAAAAGCACAGACAGCAGTATGAAGAGATGGTGGTGCAGGCTAAGAAACGAGAG
  CTTAAAGAAGCCCAGCGGCGGAAGAAACAGCTGGAGGAAAGGTGCAAGGTTGAAGAGAGCATTGGAAACG
  CCGTCCTCACCTGGAATAATGAGATCTTGCCTAACTGGGAAACCATGTGGTGCTCTAAGAAGGTTCGAGA
  CTTGTGGTGGCAGGGAATCCCTCCGAGTGTAAGAGGCAAAGTTTGGAGCTTGGCCATTGGCAACGAGCTG
  AACATCACTCACGAGCTCTTTGACATCTGTCTTGCTCGGGCCAAGGAGCGGTGGCGGTCCCTCAGCACTG
  GAGGCTCAGAAGTGGAGAATGAAGATGCTGGGTTTTCAGCAGCAGACAGAGAGGCCAGTCTGGAGCTTAT
  TAAACTGGACATTTCTAGAACATTTCCTAATCTCTGCATTTTCCAGCAAGGTGGTCCATATCATGACATG
  TTGCACAGTATTTTGGGCGCTTATACTTGTTACCGGCCGGATGTGGGTTATGTTCAGGGCATGTCCTTTA
  TAGCTGCAGTGTTGATCTTGAACCTCGATACTGCAGATGCCTTTATTGCTTTTTCTAATCTTTTGAATAA
  ACCCTGTCAAATGGCGTTTTTCCGAGTGGACCATGGCCTTATGTTGACTTACTTTGCTGCATTTGAGGTA
  TTCTTTGAAGAAAACTTGCCGAAATTGTTTGCGCATTTCAAGAAAAACAACTTAACTGCAGACATCTACC
  TAATTGATTGGATTTTTACCTTGTACAGTAAGTCCCTGCCCTTGGACTTGGCCTGCCGCATCTGGGATGT
  ATTCTGTCGAGATGGAGAGGAATTTCTCTTCCGCACAGCTCTGGGCATCCTGAAGCTTTTTGAGGATATC
  CTGACCAGGATGGACTTCATCCACAGTGCCCAGTTTCTGACCAGGCTGCCTGAGGACCTGCCAGCAGACG
  ACGTGTTCGCAGCTATCTCTACAGTCCAGATGCAAAGCCGCAACAAGAAATGGGCACAGGTGCTGTCTGC
  ACTGCAGAAAGACAGCCGGGAAATGGAGAAAGGAAGCCCATCACTCAGACAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR200076 representing NM_001034021
  Red=Cloning site Green=Tags(s)
  
  MTDGNLSTSMNGVALMGILDGRPGDSLQELQHLSIKAVPRSLSVPDYGPTLKLGALEDRHSLQSVDSGIP
  TLEIGNPEPVPCSVVHVKRKQSESEIIPERAFQSACPLPSCTPSAPTGSEREQAVRKSSTFPRTGYDSVK
  LYSPTSKALSRSDDVSVCSVSSLGTELSTTLSVSNEDILDLMVTSNSSAIVTLENDDDPQFTDVTLSSIK
  ETSDLHQQDCVAETEEGRKLRLLQPFSHFFTRNLLARKQNARLDRQRDLGWKLFGKVPLRETAQKDSKKT
  QKEYEDKAGRPSRPPSPKQNVRKNLDFEPLSTTALILEDRPANLPAKPAEEAQKHRQQYEEMVVQAKKRE
  LKEAQRRKKQLEERCKVEESIGNAVLTWNNEILPNWETMWCSKKVRDLWWQGIPPSVRGKVWSLAIGNEL
  NITHELFDICLARAKERWRSLSTGGSEVENEDAGFSAADREASLELIKLDISRTFPNLCIFQQGGPYHDM
  LHSILGAYTCYRPDVGYVQGMSFIAAVLILNLDTADAFIAFSNLLNKPCQMAFFRVDHGLMLTYFAAFEV
  FFEENLPKLFAHFKKNNLTADIYLIDWIFTLYSKSLPLDLACRIWDVFCRDGEEFLFRTALGILKLFEDI
  LTRMDFIHSAQFLTRLPEDLPADDVFAAISTVQMQSRNKKWAQVLSALQKDSREMEKGSPSLRH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001034021
• ORF Size: 2082 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001034021.2, NP_001029193.2
• RefSeq Size: 4721 bp
• RefSeq ORF: 2085 bp
• Locus ID: 360956
• Cytogenetics: 14q21
• MW: 78.2 kDa