Gmcl1 (NM_001010956) Rat Tagged ORF Clone
CAT#: RR200083L4
- LentiORF®
Lenti ORF clone of Gmcl1 (mGFP-tagged ORF) - Rat germ cell-less homolog 1 (Drosophila) (Gmcl1), transcript variant 2, (10 ug)
"NM_001010956" in other vectors (3)
Product Images
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Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | mGFP |
Symbol | Gmcl1 |
Synonyms | Gcl |
Vector | pLenti-C-mGFP-P2A-Puro |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Mammalian Cell Selection | Puromycin |
Sequence Data |
The ORF insert of this clone is exactly the same as(RR200083).
|
Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
ACCN | NM_001010956 |
ORF Size | 1479 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001010956.3, NP_001010956.1 |
RefSeq Size | 1952 bp |
RefSeq ORF | 1482 bp |
Locus ID | 312516 |
Cytogenetics | 4q34 |
Gene Summary | This gene encodes a nuclear envelope protein that contains a broad-complex, tramtrack and bric a brac/ Pox virus and Zinc finger (BTB/POZ) protein-protein interaction domain. In mouse, this gene is expressed at high levels in the testis, and null mutant mice exhibit morphologically abnormal sperm that have chromatin condensation defects. These defects result in significantly impaired fertility, demonstrating a role for this gene in male germ cell development in mouse. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2015] |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN200083 | Gmcl1 (untagged ORF) - Rat germ cell-less homolog 1 (Drosophila) (Gmcl1), transcript variant 2, (10 ug) |
USD 670.00 |
|
RR200083 | Gmcl1 (Myc-DDK-tagged ORF) - Rat germ cell-less homolog 1 (Drosophila) (Gmcl1), transcript variant 2, (10 ug) |
USD 670.00 |
|
RR200083L3 | Lenti ORF clone of Gmcl1 (Myc-DDK-tagged ORF) - Rat germ cell-less homolog 1 (Drosophila) (Gmcl1), transcript variant 2, (10 ug) |
USD 870.00 |
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