Axl (NM_001013147) Rat Tagged ORF Clone

CAT#: RR200095

Axl (Myc-DDK-tagged ORF) - Rat Axl receptor tyrosine kinase (Axl), transcript variant 2, (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Axl
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR200095 representing NM_001013147
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCAGGGTCCCGCTGGCCTGGTGCGTGGCGCTGTGCTGCTGGGGGTGTGCAGCCCCTAAGGACACAC
  AGACCGAGGCTGACAGCCCATTCGTGGGGAACCCAGGGAATATCACGGGTGCCAGAGGACTCACGGGGAC
  CCTTCGGTGTGAGCTCCAGGTTCAGGGGGAGCCCCCTGAGGTGATGTGGCTTCGAGATGGACAGATCCTA
  GAACTGGCTGATAACACCCAGACCCAGGTGCCTCTGGGCGAAGACTGGCAAGATGAATGGAAAGTCGTCA
  GTCAGCTCAGAATCTCAGCCCTGCAACTTTCAGATGCAGGAGAGTACCAGTGTATGGTGCACCTGGAAGG
  ACGGACCTTTGTGTCTCAGCCGGGCTTTGTAGGACTGGAAGGTCTCCCGTACTTCCTGGAGGAACCTGAA
  GACAAAGCTGTGCCTGCCAACACCCCCTTCAACCTAAGCTGCCAGGCCCAGGGACCCCCGGAACCCGTGA
  CCCTGCTCTGGCTTCAAGATGCTGTCCCTCTGGCCCCAGTCGCAGGATACAGCTTTCAGCACAGTTTGCA
  AGCTCCAGGCCTGAACAAGACATCTTCTTTCTCATGTGAAGCCCACAATGCCAAGGGAGTCACCACCTCC
  CGCACAGCTACCATCACAGTGCTCCCACAGAGACCTCACAATCTCCACGTGGTTTCCAGACATCCCACGG
  AGCTAGAGGTAGCTTGGATCCCTACCCTGAGTGGCATCTACCCGCTCACCCACTGCACCCTGCAGGCTGT
  GCTGTCAAACGATGGGGTGGGCGTCTGGCTGGGAAAGTCAGATCCTCCTGAAGAACCCCTCACCGTGCAA
  GTATCAGTGCCCCCCCACCAGCTTCGGCTGGAAAAGCTCCTTCCTCACACCCCATATCACATCCGGGTAT
  CCTGCACTAGCAGCCAGGGCCCCTCACCTTGGACCCACTGGCTTCCTGTGGAGACCACGGAGGGAGTGCC
  CTTGGGTCCCCCTGAGAACGTTAGCGCCATGCGGAATGGGAGCCAGGCCCTCGTGCGTTGGCAGGAGCCA
  AGGGAGCCCTTGCAGGGCACCCTGTTAGGGTACCGGCTGGCATATCGAGGCCAGGACACCCCCGAGGTAC
  TTATGGATATAGGGCTAACTCGAGAGGTGACCTTGGAACTTCGGGGGGACAGGCCTGTGGCTAACCTGAC
  TGTGTCTGTGGCAGCCTATACCTCAGCTGGGGATGGGCCCTGGAGCCTTCCTGTGCCCCTAGAGCCCTGG
  CGCCCAGTGAGTGAACCCCCACCTCCCGCCTTCTCGTGGCCTTGGTGGTATGTACTGCTGGGAGCACTTG
  TGGCCGCCGCCTGTGTCCTTATCTTGACCCTGTTCCTTGTCCATCGGAGGAAGAAGGAGACGAGATATGG
  GGAGGTGTTCGAGCCAACTGTGGAAAGGGGTGAACTGGTAGTCAGGTACCGTGCCCGAAAGTCCTACAGT
  CGCCGGACCACGGAAGCCACCTTGAACAGTCTGGGCATCAGCGAAGAGCTGAAGGAGAAACTACGAGACG
  TCATGGTAGATCGGCATAAGGTGGCCTTGGGGAAGACCCTGGGAGAGGGAGAATTTGGTGCTGTGATGGA
  GGGCCAGCTCAACCAGGATGACTCCATCCTCAAGGTCGCTGTGAAGACCATGAAAATTGCCATCTGCACA
  AGATCAGAGCTGGAGGATTTCCTGAGTGAAGCTGTCTGCATGAAGGAATTTGACCACCCCAACGTCATGA
  GGCTCATTGGCGTCTGTTTCCAGGGTTCTGACCGAGAGGGTTTCCCAGAACCGGTGGTCATCTTGCCTTT
  CATGAAACATGGAGACCTCCACAGTTTCCTCCTGTACTCGCGGCTCGGGGACCAGCCAGTGTTCCTGCCC
  ACTCAGATGCTAGTGAAGTTTATGGCCGACATTGCCAGTGGCATGGAGTACCTCAGTACCAAGAGATTCA
  TACACCGGGACCTAGCTGCCAGGAACTGCATGCTGAATGAGAACATGTCCGTGTGCGTGGCAGACTTCGG
  GCTCTCCAAGAAGATCTACAATGGGGATTACTACCGCCAAGGGCGCATTGCCAAGATGCCAGTCAAGTGG
  ATTGCTATCGAGAGTCTGGCAGATCGAGTCTACACCAGCAAGAGTGACGTGTGGTCCTTCGGTGTGACAA
  TGTGGGAGATCGCCACCCGAGGCCAAACTCCCTATCCAGGGGTGGAGAACAGTGAGATTTACGACTACCT
  ACGTCAAGGAAATCGCCTGAAACAGCCTCTGGACTGTCTGGATGGCCTCTATGCCCTGATGTCCCGGTGC
  TGGGAGCTGAACCCTCGAGACCGGCCAAGTTTTGCAGAGCTCCGGGAAGACTTGGAGAACACATTGAAGG
  CTCTACCCCCTGCTCAGGAGCCTGATGAAATCCTCTATGTCAACATGGATGAGGGCGGAAGTCACCTTGA
  ACCCCGTGGGGCTGCTGGAGGAGCTGACCCCCCAACCCAACCTGATCCTAAGGATTACTGTAGCTGTCTC
  ACTGCAGCTGACGTCCACTCAGCTGGACGCTATGTCCTTTGTCCTTCTACAGCCCCAGGACCCACTCTGT
  CTGCTGACAGAGGCTGCCCAGCACCTCCAGGGCAGGAGGACGGAGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR200095 representing NM_001013147
  Red=Cloning site Green=Tags(s)
  
  MGRVPLAWCVALCCWGCAAPKDTQTEADSPFVGNPGNITGARGLTGTLRCELQVQGEPPEVMWLRDGQIL
  ELADNTQTQVPLGEDWQDEWKVVSQLRISALQLSDAGEYQCMVHLEGRTFVSQPGFVGLEGLPYFLEEPE
  DKAVPANTPFNLSCQAQGPPEPVTLLWLQDAVPLAPVAGYSFQHSLQAPGLNKTSSFSCEAHNAKGVTTS
  RTATITVLPQRPHNLHVVSRHPTELEVAWIPTLSGIYPLTHCTLQAVLSNDGVGVWLGKSDPPEEPLTVQ
  VSVPPHQLRLEKLLPHTPYHIRVSCTSSQGPSPWTHWLPVETTEGVPLGPPENVSAMRNGSQALVRWQEP
  REPLQGTLLGYRLAYRGQDTPEVLMDIGLTREVTLELRGDRPVANLTVSVAAYTSAGDGPWSLPVPLEPW
  RPVSEPPPPAFSWPWWYVLLGALVAAACVLILTLFLVHRRKKETRYGEVFEPTVERGELVVRYRARKSYS
  RRTTEATLNSLGISEELKEKLRDVMVDRHKVALGKTLGEGEFGAVMEGQLNQDDSILKVAVKTMKIAICT
  RSELEDFLSEAVCMKEFDHPNVMRLIGVCFQGSDREGFPEPVVILPFMKHGDLHSFLLYSRLGDQPVFLP
  TQMLVKFMADIASGMEYLSTKRFIHRDLAARNCMLNENMSVCVADFGLSKKIYNGDYYRQGRIAKMPVKW
  IAIESLADRVYTSKSDVWSFGVTMWEIATRGQTPYPGVENSEIYDYLRQGNRLKQPLDCLDGLYALMSRC
  WELNPRDRPSFAELREDLENTLKALPPAQEPDEILYVNMDEGGSHLEPRGAAGGADPPTQPDPKDYCSCL
  TAADVHSAGRYVLCPSTAPGPTLSADRGCPAPPGQEDGA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001013147
• ORF Size: 2637 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001013147.1, NP_001013165.1
• RefSeq Size: 2640 bp
• RefSeq ORF: 2640 bp
• Locus ID: 308444
• Cytogenetics: 1q21
• MW: 97.2 kDa