Arhgef7 (NM_053740) Rat Tagged ORF Clone

CAT#: RR200232

Arhgef7 (Myc-DDK-tagged ORF) - Rat Rho guanine nucleotide exchange factor (GEF7) (Arhgef7), transcript variant 3, (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Arhgef7
• Synonyms: P85spr; Pak3bp
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR200232 representing NM_053740
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACTGATAACGCCAACAGCCAACTGGTAGTACGAGCCAAGTTTAACTTCCAGCAGACCAATGAAGATG
  AGCTCTCCTTCTCGAAGGGTGACGTCATCCATGTCACTCGAGTGGAGGAAGGAGGCTGGTGGGAAGGCAC
  ACACAATGGCAGGACCGGCTGGTTCCCCAGCAACTACGTACGAGAGATCAAGCCAAGTGAGAAGCCTGTG
  TCACCCAAATCAGGGACCTTGAAGAGCCCTCCCAAAGGGTTCGATACGACTGCCATCAACAAGAGCTATT
  ACAATGTGGTGCTACAGAACATCCTGGAAACAGAGCATGAGTATTCCAAGGAGCTGCAGTCTGTGCTGTC
  AACCTACCTGTGGCCGCTGCAGACCAGCGAGAAGTTGAGTTCAGCAAACACTTCATATTTAATGGGAAAT
  CTAGAGGAAATATCTTCCTTCCAGCAAGTGCTTGTACAGTCCCTAGAAGAATGCACAAAGTCTCCTGAAG
  CCCAGCAGAGAGTCGGTGGCTGCTTCCTGAGCCTGATGCCGCAGATGAGGACCCTGTACCTTGCCTACTG
  TGCCAACCACCCATCTGCTGTGAGCGTCCTCACAGAGCACAGTGAGGACCTGGGAGAGTTCATGGAGACA
  AAAGGTGCCAGCAGCCCTGGGATCCTGGTGCTGACTACCGGCCTGAGCAAGCCCTTCATGCGCCTGGACA
  AGTACCCCACACTGCTGAAGGAGCTGGAGAGACACATGGAGGATTATCATCCTGATAGACAAGATATTCA
  GAAATCTATGACTGCCTTCAAAAACCTTTCAGCCCAGTGTCAAGAAGTACGAAAAAGGAAAGAGCTGGAG
  CTGCAGATCCTGACAGAGCCCATCAGGAGCTGGGAGGGGGATGACATAAAGACGTTGGGCAGTGTTACAT
  ACATGTCCCAAGTCACAATTCAGTGTGCAGGAAGCGAGGAGAAGAATGAGAGGTACCTCCTGCTCTTCCC
  AAACCTTCTGCTCATGTTGTCTGCCAGTCCCAGGATGAGCGGTTTCATCTATCAGGGAAAGCTGCCAACA
  ACAGGAATGACAATCACAAAACTCGAGGACAGTGAGAACCATAGGAATGCATTTGAGATATCAGGGAGCA
  TGATCGAGCGCATCCTGGTGTCCTGCAACAACCAACAGGACTTGCACGAATGGGTGGAGCATCTGCAGAG
  GCAGACGAAGGTCACGTCTGTGAGCAACCCCACCATCAAGCCCCACTCGGTGCCATCGCACACACTTCCT
  TCCCATCCTCTCACTCCATCCAGCAAACACGCGGACAGCAAGCCTGTGGCACTGACGCCTGCGTACCACA
  CACTCCCCCACCCCTCTCACCATGGCACCCCACATACCACCATCAGCTGGGGACCCCTGGAGCCTCCGAA
  GACCCCCAAGCCTTGGAGCCTGAGTTGCCTGCGGCCTGCCCCTCCCCTCCGGCCCTCAGCTGCTCTCTGC
  TACAAGGAGGATCTCAGCAGGAGTCCCAAGACCATGAAAAAGCTGCTGCCAAAACGCAAGCCCGAGCGCA
  AGCCTTCAGACGAGGAGTTCGCTGTGCGCAAGAGCACAGCCGCTCTGGAGGAAGACGCTCAGATCCTGAA
  GGTTATTGAAGCTTACTGCACAAGTGCAAAGACGAGGCAGACCCTGAACTCAACATGGCAAGGCACTGAC
  CTGATGCATAATCACGTCTTGGCTGATGACGACCAATCAAGTCTAGACTCCTTGGGTCGTCGCAGTAGCC
  TTTCTCGTTTGGAGCCCTCAGACCTCTCGGAAGACTCTGAGTATGACAGTCTATGGACAGCCCATAGTTA
  CAGAATGGGTTCTGCATCCCGTAAGAGCTGCTGCTCATCTATCTCTCACCAGAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR200232 representing NM_053740
  Red=Cloning site Green=Tags(s)
  
  MTDNANSQLVVRAKFNFQQTNEDELSFSKGDVIHVTRVEEGGWWEGTHNGRTGWFPSNYVREIKPSEKPV
  SPKSGTLKSPPKGFDTTAINKSYYNVVLQNILETEHEYSKELQSVLSTYLWPLQTSEKLSSANTSYLMGN
  LEEISSFQQVLVQSLEECTKSPEAQQRVGGCFLSLMPQMRTLYLAYCANHPSAVSVLTEHSEDLGEFMET
  KGASSPGILVLTTGLSKPFMRLDKYPTLLKELERHMEDYHPDRQDIQKSMTAFKNLSAQCQEVRKRKELE
  LQILTEPIRSWEGDDIKTLGSVTYMSQVTIQCAGSEEKNERYLLLFPNLLLMLSASPRMSGFIYQGKLPT
  TGMTITKLEDSENHRNAFEISGSMIERILVSCNNQQDLHEWVEHLQRQTKVTSVSNPTIKPHSVPSHTLP
  SHPLTPSSKHADSKPVALTPAYHTLPHPSHHGTPHTTISWGPLEPPKTPKPWSLSCLRPAPPLRPSAALC
  YKEDLSRSPKTMKKLLPKRKPERKPSDEEFAVRKSTAALEEDAQILKVIEAYCTSAKTRQTLNSTWQGTD
  LMHNHVLADDDQSSLDSLGRRSSLSRLEPSDLSEDSEYDSLWTAHSYRMGSASRKSCCSSISHQN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_053740
• ORF Size: 1875 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_053740.1, NM_053740.2, NP_446192.2
• RefSeq Size: 1878
• RefSeq ORF: 1878
• Locus ID: 114559
• MW: 70.3 kDa
• Gene Summary: Rac/Cdc42-specific guanine nucleotide exchange factor; mediates bFGF-induced neurite outgrowth [RGD, Feb 2006]