Bag6 (NM_001033968) Rat Tagged ORF Clone

CAT#: RR200239

Bag6 (Myc-DDK-tagged ORF) - Rat HLA-B-associated transcript 3 (Bat3), transcript variant 1, (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Bag6
• Synonyms: Bat3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR200239 representing NM_001033968
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCCGAGTGATAGTACCAGTACCGCTATGGAGGAGCCTGACAGCCTGGAGGTACTGGTGAAGACCC
  TGGACTCTCAGACTCGGACTTTTATTGTGGGGGCCCAGATGAATGTAAAGGAGTTTAAGGAACACATAGC
  TGCCTCTGTCAGCATCCCTTCCGAGAAACAGCGGCTCATCTACCAGGGCCGGGTTCTACAAGATGACAAG
  AAGCTCCAGGACTACAATGTTGGGGGAAAGGTTATCCACCTGGTGGAACGGGCTCCTCCTCAGACTCAGC
  TACCTTCTGGAGCATCTTCTGGGACAGGCTCTGCCTCAGCTACTCATGGTGGGGGCCCCCTGCCTGGCAC
  TCGGGGCCCTGGGGCCTCTGGTCATGACCGGAATGCCAACAGTTATGTCATGGTTGGAACCTTCAATCTT
  CCTAGTGACGGCTCTGCTGTGGACGTTCACATCAACATGGAACAGGCCCCAATTCAGAGTGAGCCCCGGG
  TACGGCTGGTGATGGCTCAGCACATGATTAGGGATATACAGACCTTACTGTCCCGGATGGAGTGTCGAGG
  GGGAACCCAAGCACAGGCCAGTCAGCCACCCCCGCAGACTCCGACTGTGGCCTCAGAGACAGTAGCTTTG
  AACTCACAAACATCAGAGCCAGTTGAAAGTGAAGCTCCTCCTCGAGAGCCCATGGAGTCAGAAGAAATGG
  AGGAACGTCCCCCAACCCAGACTCCAGAGCTTCCACCCTCTGGCCCAGCTCCAGCAGGCCCAGCACCCGC
  ACCAGAGACAAATGCACCCAACCACCCTTCCCCTGCAGAGCACGTGGAGGTGCTCCAGGAGCTTCAGCGC
  TTGCAACGCCGTCTTCAGCCCTTCCTGCAGCGCTACTGTGAGGTCCTGGGTGCTGCTGCCACTACAGACT
  ACAATAACAATCATGAGGGCCGAGAAGAGGACCAGAGGTTGATTAACTTGGTGGGGGAGAGCCTGCGGCT
  ACTGGGCAACACTTTCGTGGCATTGTCTGATCTGCGCTGTAATCTAGCCTGTGCACCCCCACGGCACCTG
  CATGTGGTACGGCCTATGTCTCACTACACGACTCCCATGGTGCTCCAGCAGGCAGCCATTCCCATTCAGA
  TCAATGTGGGGACTACTGTGACCATGACAGGCAATGGGGCTCGGCCTCCACCAGCTCCCGGTGCAGAGGC
  AGCATCCCCAGGTTCTGGCCAGGCCTCATCCCTTCCTCCATCTTCTGCCACTGTTGATTCATCAACGGAA
  GGAGCTCCCCCACCAGGGCCAGCACCACCACCAGCCACCAGCCATCCACGGGTCATCCGGATTTCCCACC
  AGAGTGTGGAACCTGTGGTCATGATGCACATGAACATTCAAGACTCCGGAGCACAGCCTGGAGGTGTGCC
  CAGTGCTCCCACTGGCCCCCTGGGACCTCCTGGTCATGGACAGAGCCTGGGACAGCAGGTGCCCGGCTTC
  CCAACAGCACCAACTCGGGTGGTGATTGCCCGACCCACTCCTCCACAGGCTCGGCCTTCCCATCCTGGGG
  GCCCTCCAGTCTCTGGAGCTCTACAGGGTGCTGGGCTGGGTACAAACACTTCATTGGCCCAGATGGTGAG
  CGGCCTTGTGGGGCAACTTCTCATGCAGCCTGTTCTTGTGGCTCAGGGGACTCCAGGAATGGCTCCGGCT
  TCTGCTTCAGCTCCGGCTACGGCCCAGGCACAGGCCCCGGCCCCAGCCCCAGCTCCGGCCCCAGCTCCGG
  CCACTGCTTCAGCTAGTGCTGGCACTACCAACACAGCCACCACAGCCGGCCCTGCTCCTGGGGGTCCCGC
  CCAGCCTCCACCTCCTCAGCCCTCTGCGGCTGATCTTCAGTTCTCTCAGCTCCTGGGAAATCTGCTGGGG
  CCTGCAGGGCCAGGGGCTGGGGGCCCAAGCCTGGCTTCTCCCACCATCACTGTGGCAGTGCCTGGAGTTC
  CTGCTTTTCTCCAGGGCATGACTGAGTTTTTGCAGGCATCACAGGCTGCCCCTCCACCCCCTCCACCTCC
  TCCACCCCCACCTCCTGCTCCAGAGCAGCAGACCACACCGCCTCCAGGATCCCCTTCTGGTGGGACAGCG
  AGTCCTGGGGGCTTAGGTCCTGAGAGCCTGCCACCAGAGTTTTTCACCTCAGTGGTGCAGGGCGTGTTGA
  GCTCTCTCCTGGGCTCCTTGGGGGCTCGTGCTGGCAGCAGCGAGAGTATTGCTGCCTTCATCCAACGCCT
  CAGTGGATCCAGCAACATCTTTGAGCCTGGGGCTGATGGCGCCCTTGGATTCTTTGGAGCTCTGCTCTCT
  CTTCTGTGCCAGAATTTCTCCATGGTGGATGTGGTGATGCTTCTCCATGGCCATTTCCAGCCACTACAGC
  GGCTCCAGCCACAGCTACGATCTTTCTTCCACCAGCACTACCTGGGTGGCCAGGAGCCCACGTCCAGCAA
  CATCCGGATGGCAACCCACACGTTGATCACGGGGCTGGAAGAGTATGTACGGGAGAGTTTCTCTTTGGTG
  CAGGTTCAGCCAGGTGTGGATATCATCCGGACAAATTTAGAATTTCTCCAGGAGCAATTTAACAGCATTG
  CTGCTCACGTGCTGCACTGTACAGACAGTGGATTTGGAGCCCGGCTGCTGGAGCTATGTAACCAGGGCCT
  GTTTGAATGCTTGGCCCTGAACCTGCACTGCTTGGGGGGCCAGCAAATGGAGCTTGCTGCTGTCATCAAT
  GGCCGAATTCGCCGCATGTCTCGTGGGGTGAATCCATCCTTGGTGAGCTGGCTGACAACTATGATGGGAC
  TCAGGCTTCAGGTGGTCTTAGAGCACATGCCCGTGGGTCCCGACGCCATCCTCAGATATGTGCGCAGGAT
  TGGTGATCCTCCTCAGGCACTTCCTGAAGAGCCAATGGAAGTTCAGGGAGCAGAAAGAACTTCCCCTGAG
  CCTCAGAGAGAGGATGCTTCCCCAGCCCCCGGAACAACAGCAGAAGAAGCCATGTCCCGAGGTCCACCCC
  CTGCTCCTGAAGGAGGTTCCCGAGACGAACAGGATGGAGCTTCAGCCGATGCAGAGCCCTGGGCAGCTGC
  AGTCCCCCCTGAATGGGTACCTATTATCCAGCAGGACATTCAGAGCCAGCGGAAGGTGAAACCTCAGCCG
  CCCCTGAGTGATGCCTACCTCAGTGGCATGCCTGCCAAGAGACGAAAGACAATGCAGGGTGAGGGCCCCC
  AGCTGCTACTCTCAGAGGCAGTGAGCCGGGCAGCTAAGGCAGCCGGAGCTCGGCCCCTGACAAGCCCCGA
  GAGCCTGAGCCGGGACCTGGAGGCACCAGAGGTTCAGGAGAGCTACAGGCAGCAGCTCCGGTCTGATATT
  CAAAAACGACTGCAGGAAGATCCCAACTACAGCCCCCAGCGCTTCCCTAATGCCCACCGGGCATTTGCTG
  ATGACCCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR200239 representing NM_001033968
  Red=Cloning site Green=Tags(s)
  
  MEPSDSTSTAMEEPDSLEVLVKTLDSQTRTFIVGAQMNVKEFKEHIAASVSIPSEKQRLIYQGRVLQDDK
  KLQDYNVGGKVIHLVERAPPQTQLPSGASSGTGSASATHGGGPLPGTRGPGASGHDRNANSYVMVGTFNL
  PSDGSAVDVHINMEQAPIQSEPRVRLVMAQHMIRDIQTLLSRMECRGGTQAQASQPPPQTPTVASETVAL
  NSQTSEPVESEAPPREPMESEEMEERPPTQTPELPPSGPAPAGPAPAPETNAPNHPSPAEHVEVLQELQR
  LQRRLQPFLQRYCEVLGAAATTDYNNNHEGREEDQRLINLVGESLRLLGNTFVALSDLRCNLACAPPRHL
  HVVRPMSHYTTPMVLQQAAIPIQINVGTTVTMTGNGARPPPAPGAEAASPGSGQASSLPPSSATVDSSTE
  GAPPPGPAPPPATSHPRVIRISHQSVEPVVMMHMNIQDSGAQPGGVPSAPTGPLGPPGHGQSLGQQVPGF
  PTAPTRVVIARPTPPQARPSHPGGPPVSGALQGAGLGTNTSLAQMVSGLVGQLLMQPVLVAQGTPGMAPA
  SASAPATAQAQAPAPAPAPAPAPATASASAGTTNTATTAGPAPGGPAQPPPPQPSAADLQFSQLLGNLLG
  PAGPGAGGPSLASPTITVAVPGVPAFLQGMTEFLQASQAAPPPPPPPPPPPPAPEQQTTPPPGSPSGGTA
  SPGGLGPESLPPEFFTSVVQGVLSSLLGSLGARAGSSESIAAFIQRLSGSSNIFEPGADGALGFFGALLS
  LLCQNFSMVDVVMLLHGHFQPLQRLQPQLRSFFHQHYLGGQEPTSSNIRMATHTLITGLEEYVRESFSLV
  QVQPGVDIIRTNLEFLQEQFNSIAAHVLHCTDSGFGARLLELCNQGLFECLALNLHCLGGQQMELAAVIN
  GRIRRMSRGVNPSLVSWLTTMMGLRLQVVLEHMPVGPDAILRYVRRIGDPPQALPEEPMEVQGAERTSPE
  PQREDASPAPGTTAEEAMSRGPPPAPEGGSRDEQDGASADAEPWAAAVPPEWVPIIQQDIQSQRKVKPQP
  PLSDAYLSGMPAKRRKTMQGEGPQLLLSEAVSRAAKAAGARPLTSPESLSRDLEAPEVQESYRQQLRSDI
  QKRLQEDPNYSPQRFPNAHRAFADDP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001033968
• ORF Size: 3438 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001033968.1, NP_001029140.1
• RefSeq Size: 3651 bp
• RefSeq ORF: 3441 bp
• Locus ID: 94342
• Cytogenetics: 20p12
• MW: 120 kDa