Pde2a (NM_001143847) Rat Tagged ORF Clone

CAT#: RR200272

Pde2a (Myc-DDK-tagged ORF) - Rat phosphodiesterase 2A, cGMP-stimulated (Pde2a), transcript variant 1, (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Pde2a
• Synonyms: CGS-PDE; Pde2; Pde2a2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR200272 representing NM_001143847
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGCAGGCATGCGGCCACTCCATCCTCTGCAGGAGCCAGCAGTACCCGGCAGCGCGACCGGCTGAGC
  CGCGGGGCCAGCAGGTCTTCCTCAAGCCGGACGAACCGCCGCCGCAGCCATGCGCCGACAGCCTGCAGGA
  TGCTTTGCTGAGCCTGGGCGCCGTTATCGACATCGCGGGCCTTCGACAGGCTGCCAAGGATGCCCTCTCG
  GCCGTGCTCCCCAAAGTGGAGACTGTCTACACCTACCTGGTAGATGGGGAGTCCAGGCTGGTGTGTGAGG
  ACCCCCCTCATGAGCTGCCACAGGAAGGAAAAATTCGAGAGGCTGTGATTTCTCGGAAGCGGCTGAGCTG
  CGATGGGCTGGGACCTTCAGACCTACTAGGAAAGCCCTTGGCCAGGCTGGTGGCTCCACTGGCCCCTGAC
  ACGCAAGTGCTGGTCATACCCCTGCTGGACAAGGAGACTGGAACTGTGGCAGCCGTCATCTTGGTGCACT
  GTGGCCAGCTGAGTGACAGTGAGGAGCAGAGCCTGCAGGTGGTGGAGAAGCATGCTCTGGTGGCCCTGCA
  GAGGGTGCAGGCCCTGCAGCAGCGCAGGCCTGAAGCTGTTCAGAACACCTCAGCGGATCCCTCTGAAGAT
  CAAAAGGATGAGAAGGGATACACCGCCCATGACCGAAAGATCCTGCAACTGTGTGGAGAACTCTATGACT
  TGGATGCCACTTCTCTGCAGCTCAAAGTCCTTCGATATCTTCAGCAGGAGACACAGGCCACTCACTGCTG
  CCTCCTGCTGGTGTCAGAGGACAACCTGCAGCTTTCCTGCAAGGTCATTGGAGAGAAAGTGCTGGGAGAA
  GAGGTCAGCTTTCCATTGACCATGGGACGCCTGGGCCAGGTGGTGGAGGACAAACAGTGTATCCAGTTGA
  AGGACCTAACCTCTGACGATGTGCAACAGCTACAAAACATGTTGGGCTGTGAGCTTCGGGCTATGCTATG
  TGTCCCTGTCATCAGTCGAGCCACTGACCAGGTGGTGGCCCTGGCTTGCGCCTTCAACAAGCTTGGAGGA
  GACTTCTTCACAGATGAGGATGAACGTGCGATCCAACACTGCTTCCACTACACAGGCACGGTGCTCACCA
  GTACCTTGGCCTTCCAGAAGGAGCAGAAGCTCAAGTGTGAGTGCCAGGCTCTTCTCCAAGTGGCAAAGAA
  CCTCTTCACCCACCTGGATGACGTCTCTGTCCTGCTACAGGAGATCATCACAGAGGCCAGAAACCTCAGC
  AACGCAGAGATCTGCTCCGTGTTCCTGCTGGATCAGAATGAGCTGGTTGCCAAGGTGTTCGATGGCGGTG
  TAGTGGACGATGAGAGTTATGAGATCCGCATCCCTGCGGACCAAGGCATCGCGGGCCACGTGGCGACCAC
  GGGCCAGATCCTGAACATCCCAGATGCATACGCCCATCCGCTTTTCTATCGCGGCGTAGACGACAGCACT
  GGCTTCCGCACGCGCAACATTCTCTGCTTCCCTATCAAGAACGAGAACCAAGAGGTCATCGGTGTGGCTG
  AGCTAGTGAACAAGATCAATGGGCCATGGTTCAGCAAATTTGATGAGGACCTGGCCACAGCCTTCTCCAT
  CTACTGTGGCATTAGCATCGCTCACTCTCTCCTATACAAAAAGGTGAATGAAGCCCAGTACCGCAGCCAC
  CTGGCCAACGAGATGATGATGTACCATATGAAGGTCTCTGACGATGAATACACCAAACTTCTCCATGATG
  GCATCCAACCTGTGGCCGCCATTGACTCCAACTTTGCCAACTTCACCTATACTCCTCGGTCTCTGCCTGA
  GGACGATACTTCTATGGCCATCCTGAGCATGCTGCAAGACATGAACTTCATCAATAACTACAAAATTGAC
  TGCCCAACTCTGGCCCGGTTCTGCCTGATGGTGAAGAAAGGCTACCGGGATCCGCCCTACCACAACTGGA
  TGCACGCCTTCTCTGTCTCTCACTTTTGCTACTTGCTCTACAAGAATCTGGAGCTCTCCAACTACCTCGA
  GGACATCGAGATCTTTGCGTTGTTTATTTCCTGCATGTGTCACGACCTGGACCACAGAGGCACAAACAAC
  TCCTTCCAGGTGGCCTCGAAATCTGTGCTGGCTGCACTCTACAGCTCAGAGGGCTCTGTCATGGAGAGGC
  ACCACTTTGCTCAGGCCATTGCTATCCTCAACACCCATGGCTGTAATATCTTTGACCACTTCTCTCGGAA
  GGACTATCAGCGAATGCTGGACCTGATGAGGGACATCATCTTGGCCACGGACCTGGCACACCACCTCCGC
  ATCTTCAAGGACCTGCAGAAGATGGCTGAAGTGGGTTATGACCGAAACAACAAGCAACACCACAGGCTTC
  TTCTATGCCTCCTTATGACCTCCTGTGACCTCTCTGACCAGACAAAGGGCTGGAAGACCACCAGAAAGAT
  TGCGGAGCTGATCTACAAAGAGTTCTTCTCCCAAGGAGACTTGGAGAAGGCCATGGGCAACCGACCAATG
  GAGATGATGGATCGGGAGAAGGCCTACATCCCTGAACTTCAGATCAGCTTTATGGAGCACATCGCCATGC
  CCATCTACAAGCTGTTACAAGACCTGTTCCCCAAGGCAGCAGAGCTGTATGAACGCGTGGCCTCCAATCG
  TGAGCACTGGACCAAGGTGTCCCACAAGTTCACCATCCGAGGCCTCCCCAGTAACAACTCGCTGGATTTC
  CTGGATGAGGAATATGAGGTCCCTGATTTGGACGTCACCAGGGCTCCTGTCAATGGCTGCTGCAGCCTCG
  AGGGC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR200272 representing NM_001143847
  Red=Cloning site Green=Tags(s)
  
  MGQACGHSILCRSQQYPAARPAEPRGQQVFLKPDEPPPQPCADSLQDALLSLGAVIDIAGLRQAAKDALS
  AVLPKVETVYTYLVDGESRLVCEDPPHELPQEGKIREAVISRKRLSCDGLGPSDLLGKPLARLVAPLAPD
  TQVLVIPLLDKETGTVAAVILVHCGQLSDSEEQSLQVVEKHALVALQRVQALQQRRPEAVQNTSADPSED
  QKDEKGYTAHDRKILQLCGELYDLDATSLQLKVLRYLQQETQATHCCLLLVSEDNLQLSCKVIGEKVLGE
  EVSFPLTMGRLGQVVEDKQCIQLKDLTSDDVQQLQNMLGCELRAMLCVPVISRATDQVVALACAFNKLGG
  DFFTDEDERAIQHCFHYTGTVLTSTLAFQKEQKLKCECQALLQVAKNLFTHLDDVSVLLQEIITEARNLS
  NAEICSVFLLDQNELVAKVFDGGVVDDESYEIRIPADQGIAGHVATTGQILNIPDAYAHPLFYRGVDDST
  GFRTRNILCFPIKNENQEVIGVAELVNKINGPWFSKFDEDLATAFSIYCGISIAHSLLYKKVNEAQYRSH
  LANEMMMYHMKVSDDEYTKLLHDGIQPVAAIDSNFANFTYTPRSLPEDDTSMAILSMLQDMNFINNYKID
  CPTLARFCLMVKKGYRDPPYHNWMHAFSVSHFCYLLYKNLELSNYLEDIEIFALFISCMCHDLDHRGTNN
  SFQVASKSVLAALYSSEGSVMERHHFAQAIAILNTHGCNIFDHFSRKDYQRMLDLMRDIILATDLAHHLR
  IFKDLQKMAEVGYDRNNKQHHRLLLCLLMTSCDLSDQTKGWKTTRKIAELIYKEFFSQGDLEKAMGNRPM
  EMMDREKAYIPELQISFMEHIAMPIYKLLQDLFPKAAELYERVASNREHWTKVSHKFTIRGLPSNNSLDF
  LDEEYEVPDLDVTRAPVNGCCSLEG
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001143847
• ORF Size: 2805 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001143847.1, NP_001137319.1
• RefSeq Size: 4155
• RefSeq ORF: 2808
• Locus ID: 81743
• MW: 105.2 kDa
• Gene Summary: activation induces decreased cAMP accumulation; involved in nitric oxide mediated signaling in cardiac fibroblasts [RGD, Feb 2006]