Araf (NM_022532) Rat Tagged ORF Clone

CAT#: RR200318

Araf (Myc-DDK-tagged ORF) - Rat v-raf murine sarcoma 3611 viral oncogene homolog (Araf), transcript variant 1, (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Araf
• Synonyms: Araf1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR200318 representing NM_022532
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAACCACCACGAGGCCCTCCTGCTAGTGGGGCTGAGCCATCTCGGGCAGTTGGCACTGTCAAAGTGT
  ACCTGCCTAACAAGCAACGCACAGTGGTGACTGTCCGGGATGGCATGAGTGTCTATGACTCTTTGGACAA
  GGCCCTCAAGGTGCGGGGTCTCAATCAGGACTGCTGTGTGGTCTACAGACTCATCAAAGGAAGAAAGACA
  GTCACTGCCTGGGACACAGCCATTGCACCTCTGGATGGCGAGGAGCTCATTGTGGAGGTCCTGGAAGATG
  TGCCACTGACCATGCACAATTTTGTACGGAAGACATTCTTCAGTTTGGCCTTCTGTGACTTCTGCCTTAA
  GTTTCTGTTCCACGGCTTTCGCTGCCAAACCTGTGGCTACAAGTTCCACCAGCATTGTTCCTCCAAGGTC
  CCCACGGTCTGCGTTGACATGAGTACCAACCGCCGACAGTTCTACCACAGCATCCAGGATTTGTCTGGAG
  GCTCCAGGCAGCAGGAGGTTCCCTCAAATCTCTCTGTGAATGAGCTGCTAACCCCCCAGGGTCCCAGTCC
  CTTTACCCAGCAACGTGACCAGGAGCACTTCTCCTTCCCTGCCCCTGCCAATCCCCCACTGCAGCGCATC
  CGCTCCACATCTACTCCTAACGTCCACATGGTCAGCACCACAGCTCCCATGGACTCCAGCCTCATGCAGT
  TTACTGCTCAGAGCTTCAGCACCGATGCTGCTGGTAGAGGTGGTGATGGAGCTCCTCGGGGTAGCCCTAG
  CCCAGCTAGTGTGTCTTCAGGGAGGAAGTCCCCACATTCCAAGTTACCTGCAGAACAGCGGGAACGGAAG
  TCCTTGGCAGATGAAAAGAAAAAAGTGAAGAACCTGGGGTACCGGGACTCAGGCTATTACTGGGAGGTGC
  CACCCAGTGAGGTACAGCTGTTGAAGAGGATCGGGACAGGCTCTTTTGGCACTGTGTTTCGGGGGCGTTG
  GCATGGCGATGTAGCTGTGAAAGTGCTCAAGGTGGCCCAACCTACCGCTGAGCAGGCCCAGGCCTTCAAG
  AATGAGATGCAGGTGCTCAGGAAGACACGACATGTCAACATTTTGCTGTTTATGGGTTTCATGACTCGGC
  CAGGGTTTGCCATCATCACACAGTGGTGTGAGGGTTCCAGCCTCTACCACCACCTACATGTGGCTGACAC
  GCGCTTTGACATGGTCCAGCTCATTGATGTGGCCCGGCAGACTGCCCAGGGCATGGACTACCTCCACGCC
  AAGAACATCATTCACCGAGACCTAAAGTCCAACAATATCTTCCTACATGAGGGGCTCACAGTCAAGATTG
  GTGACTTCGGCCTGGCCACAGTGAAGACACGATGGAGTGGGGCCCAGCCCTTAGAGCAGCCCTCAGGGTC
  TGTGCTGTGGATGGCAGCTGAGGTGATCCGAATGCAGGACCCGAACCCCTACAGCTTCCAGTCGGATGTC
  TATGCCTATGGTGTTGTGCTCTATGAGCTTATGACCGGCTCACTGCCCTACAGCCACATTGGCAGCCGTG
  ACCAGATCATCTTTATGGTGGGTCGTGGCTATCTGTCTCCGGACCTCAGCAAAATCTTCAGTAATTGCCC
  CAAAGCCATGAGGCGCTTGCTGACTGACTGCCTCAAGTTCCAGCGGGAGGAGCGGCCTCTATTTCCCCAG
  ATTCTGGCCACGATCGAGCTGCTGCAGCGGTCACTCCCCAAGATTGAGCGGAGTGCCTCCGAACCCTCCT
  TGCACCGTACCCAGGCTGATGAGTTGCCTGCCTGCCTTCTCAGCGCAGCCCGCCTTGTGCCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR200318 representing NM_022532
  Red=Cloning site Green=Tags(s)
  
  MEPPRGPPASGAEPSRAVGTVKVYLPNKQRTVVTVRDGMSVYDSLDKALKVRGLNQDCCVVYRLIKGRKT
  VTAWDTAIAPLDGEELIVEVLEDVPLTMHNFVRKTFFSLAFCDFCLKFLFHGFRCQTCGYKFHQHCSSKV
  PTVCVDMSTNRRQFYHSIQDLSGGSRQQEVPSNLSVNELLTPQGPSPFTQQRDQEHFSFPAPANPPLQRI
  RSTSTPNVHMVSTTAPMDSSLMQFTAQSFSTDAAGRGGDGAPRGSPSPASVSSGRKSPHSKLPAEQRERK
  SLADEKKKVKNLGYRDSGYYWEVPPSEVQLLKRIGTGSFGTVFRGRWHGDVAVKVLKVAQPTAEQAQAFK
  NEMQVLRKTRHVNILLFMGFMTRPGFAIITQWCEGSSLYHHLHVADTRFDMVQLIDVARQTAQGMDYLHA
  KNIIHRDLKSNNIFLHEGLTVKIGDFGLATVKTRWSGAQPLEQPSGSVLWMAAEVIRMQDPNPYSFQSDV
  YAYGVVLYELMTGSLPYSHIGSRDQIIFMVGRGYLSPDLSKIFSNCPKAMRRLLTDCLKFQREERPLFPQ
  ILATIELLQRSLPKIERSASEPSLHRTQADELPACLLSAARLVP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_022532
• ORF Size: 1812 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_022532.1, NM_022532.2, NP_071977.1
• RefSeq Size: 2350
• RefSeq ORF: 1815
• Locus ID: 64363
• MW: 67.6 kDa