Gria4 (NM_001113185) Rat Tagged ORF Clone

CAT#: RR200372

Gria4 (Myc-DDK-tagged ORF) - Rat glutamate receptor, ionotropic, AMPA4 (Gria4), transcript variant 3, (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Gria4
• Synonyms: GluA4; GluR-D; GluR4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR200372 representing NM_001113185
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGATTATTTGCAGGCAGATTGTCTTGTTGTTTTCTGGATTTTGGGGACTCGCCATGGGAGCCTTTC
  CAAGCAGCGTTCAAATAGGTGGTCTCTTCATCCGAAACACAGACCAGGAATACACTGCTTTTAGACTGGC
  AATCTTTCTTCATAACACCAGCCCCAATGCATCGGAAGCTCCTTTCAATTTGGTACCTCATGTGGACAAC
  ATTGAGACTGCCAACAGTTTTGCTGTGACAAACGCCTTCTGTTCCCAGTATTCTAGAGGGGTGTTTGCCA
  TTTTTGGACTCTATGACAAGAGATCCGTGCATACCTTGACCTCGTTCTGCAGTGCTCTGCACATCTCTCT
  CATCACACCAAGCTTTCCCACTGAAGGGGAGAGCCAGTTTGTGCTGCAGCTAAGACCTTCACTGAGAGGT
  GCACTCCTGAGCCTCCTGGATCACTATGAGTGGAACTGTTTCGTCTTCCTGTATGATACAGACAGGGGGT
  ATTCAATACTTCAAGCTATAATGGAAAAAGCAGGACAAAATGGATGGCATGTCAGTGCAATATGTGTGGA
  AAATTTTAATGATGTCAGCTACAGGCAACTGCTAGAAGAGCTTGACAGAAGACAAGAGAAGAAATTTGTG
  ATAGATTGTGAGATAGAGAGGCTTCAAAACATTTTAGAACAAATTGTGAGTGTTGGGAAGCATGTCAAAG
  GCTACCATTATATCATCGCAAATTTGGGTTTCAAGGATATTTCTCTTGAGAGATTTATACATGGAGGAGC
  AAATGTAACAGGATTCCAGTTGGTAGATTTTAATACACCCATGGTAACCAAACTAATGGATCGGTGGAAG
  AAACTAGATCAGAGAGAATATCCAGGTTCTGAAACACCTCCAAAGTACACCTCTGCTCTCACTTATGATG
  GAGTCCTGGTGATGGCTGAAACTTTCCGAAGTCTCAGAAGACAGAAAATTGATATTTCAAGGAGAGGAAA
  TGCTGGGGACTGTCTGGCAAACCCTGCTGCTCCCTGGGGCCAGGGAATTGACATGGAGAGGACACTGAAG
  CAGGTTCGAATTCAAGGGCTGACTGGGAATGTTCAATTTGACCATTATGGACGTAGAGTTAATTACACAA
  TGGATGTGTTTGAACTGAAAAGCACAGGACCTCGAAAGGTTGGCTACTGGAATGATATGGATAAATTAGT
  CTTGATTCAAGATATGCCTACTCTTGGCAATGACACAGCAGCTATTGAGAACAGAACAGTGGTTGTAACC
  ACAATTATGCCTCTGATGAAGAATCCTATTTTAAGAAAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR200372 representing NM_001113185
  Red=Cloning site Green=Tags(s)
  
  MRIICRQIVLLFSGFWGLAMGAFPSSVQIGGLFIRNTDQEYTAFRLAIFLHNTSPNASEAPFNLVPHVDN
  IETANSFAVTNAFCSQYSRGVFAIFGLYDKRSVHTLTSFCSALHISLITPSFPTEGESQFVLQLRPSLRG
  ALLSLLDHYEWNCFVFLYDTDRGYSILQAIMEKAGQNGWHVSAICVENFNDVSYRQLLEELDRRQEKKFV
  IDCEIERLQNILEQIVSVGKHVKGYHYIIANLGFKDISLERFIHGGANVTGFQLVDFNTPMVTKLMDRWK
  KLDQREYPGSETPPKYTSALTYDGVLVMAETFRSLRRQKIDISRRGNAGDCLANPAAPWGQGIDMERTLK
  QVRIQGLTGNVQFDHYGRRVNYTMDVFELKSTGPRKVGYWNDMDKLVLIQDMPTLGNDTAAIENRTVVVT
  TIMPLMKNPILRN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001113185
• ORF Size: 1299 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001113185.1, NP_001106656.1
• RefSeq Size: 2966
• RefSeq ORF: 1302
• Locus ID: 29629
• MW: 49.2 kDa
• Gene Summary: Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. These receptors are heteromeric protein complexes composed of multiple subunits, arranged to form ligand-gated ion channels. The classification of glutamate receptors is based on their activation by different pharmacologic agonists. The subunit encoded by this gene belongs to a family of AMPA (alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate)-sensitive glutamate receptors, and is subject to RNA editing (AGA->GGA; R->G). Alternative splicing of this gene results in transcript variants encoding different isoforms, which may vary in their signal transduction properties. [provided by RefSeq, Jul 2008]