Hap1 (NM_177982) Rat Tagged ORF Clone

CAT#: RR200388

Hap1 (Myc-DDK-tagged ORF) - Rat huntingtin-associated protein 1 (Hap1), transcript variant 2, (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Hap1
• Synonyms: HAP1-A; HAP1-B
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR200388 representing NM_177982
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGCCCGAAGGACCAGGTGCAGAGCAGTGCCGGGGACGGGACGGGGTCGGGGGACCCAGCAACAGGCA
  CCCCCACGACCCAGCCTGCAGCGGATCCCGCTCCGGAGCCCTCGGCAGAGCCCAAACCTGCTCCGGCGCA
  GGGAACCGGGTCCGGACAGAAATCAGGATCCCGAACCAAGACAGGAGGAAGCTTTTGTAGGTCCAGGATC
  CGTGGCGACTCGGACGCACCATGGACCCGCTACATATTCCAGGGGCCTTACGGTCCCCGGGCTACTGGCC
  TGGGCACTGGAAGGGCTGAGGGAATCTGGAAGACGCCAGCCGCGTACATCGGCCGAAGGCCCGGCGTGTC
  CGGCCCTGAGCGCGCGGCGTTTATTCGAGAGCTGCAGGAAGCGCTGTGTCCTAATCCACTGCCCAGGAAG
  AAGATCACCGAAGATGATATCAAAGTGATGTTGTATTTGCTGGAAGAGAAAGAACGGGACCTGAACACAG
  CCGCTCGCATCGGCCAGTCCCTGGTGAAACAGAATAGTGTTCTGATGGAGGAGAATAACAAGCTGGAAAC
  CATGCTGGGCTCAGCCAGAGAGGAGATTTTACATCTCCGGAAGCAGGTGAACCTGCGAGATGATCTCCTT
  CAGCTCTACTCGGACTCCGATGACGATGAGGAGGATGAAGAGGATGAGGAAGAGGAAGAGGGAGAAGAGG
  AGGAACGAGAAGGACAGAGGGACCAAGATCAGCAGCACGACCATCCCTATGGTGCCCCCAAGCCGCCCCC
  TAAGGCTGAGACGCTGCACCACTGCCCACAGCTGGAAGCCCTGAAGCAGAAGCTGAAACTGCTGGAAGAA
  GAGAACGACCATCTTCGAGAGGAGGCCTCCCACCTTGACAACCTGGAAGACAAAGAACAGATGCTCATTC
  TGGAGTGTGTGGAACAGTTTTCTGAAGCCAGCCAGCAGATGGCAGAGCTATCCGAGGTGTTGGTGCTGAG
  GCTGGAAGGCTATGAGAGGCAGCAGAAGGAGATCACTCAGCTGCAGGCCGAGATCACCAAGCTACAACAG
  CGTTGTCAGTCTTATGGGGCCCAGACGGAGAAACTGCAGCAGCAGCTGGCCTCAGAGAAGGGAGTCCACC
  CAGAGAGCCTGCGAGCTGGCTCCCACATGCAGGATTATGGAAGCAGGCCTCGTGAACGCCAGGAGGATGG
  GAAGAGCCATCGTCAGCGTTCCTCAATGCCTGCAGGTTCTGTCACCCACTATGGATACAGTGTGCCTCTG
  GATGCACTTCCAAGTTTCCCAGAGACACTGGCGGAGGAGCTCCGGACATCCCTGAGGAAGTTCATCACTG
  ACCCTGCGTATTTCATGGAGAGATGTGACACTCGCTGCAGAGAGGAACGAAAGAAGGAGCAGGGGACAAT
  GCCACCCCCACCGGTGCAAGATCTCAAGCCGCCTGAAGATTTCGAGGCTCCAGAGGAGCTGGTTCCTGAG
  GAGGAGCTGGGGGCCATAGAAGAGGTGGGGACAGCTGAGGATGGGCCGGCAGAAGAGACAGAGCAGGCAT
  CTGAGGAGACCGAGGCCTGGGAGGAGGTGGAACCGGAGGTGGACGAGGCCACAAGGATGAATGTGGTGGT
  CTCTGCCCTGGAGGCCAGCGGCCTGGGCCCTTCACACCTGGACATGAAGTATGTCCTCCAGCAACTGTCC
  AACTGGCAGGACGCCCATTCTAAGCGGCAGCAGAAGCAGAAGGTGGTCCCGAAAGACTCCCCAGCCCCGC
  AGCAGCAAACAAACATGGGGGGCGGGATCGTGGAGCAGCAGCCCATAGTGCCGACCCAGGACTCTCAGAG
  GCTGGAGGAGGACAGGGCCACTCACTCTCCCAGTGCCAGGGAGGAAGAGGGGCCTTCTGGGGCCACC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR200388 representing NM_177982
  Red=Cloning site Green=Tags(s)
  
  MRPKDQVQSSAGDGTGSGDPATGTPTTQPAADPAPEPSAEPKPAPAQGTGSGQKSGSRTKTGGSFCRSRI
  RGDSDAPWTRYIFQGPYGPRATGLGTGRAEGIWKTPAAYIGRRPGVSGPERAAFIRELQEALCPNPLPRK
  KITEDDIKVMLYLLEEKERDLNTAARIGQSLVKQNSVLMEENNKLETMLGSAREEILHLRKQVNLRDDLL
  QLYSDSDDDEEDEEDEEEEEGEEEEREGQRDQDQQHDHPYGAPKPPPKAETLHHCPQLEALKQKLKLLEE
  ENDHLREEASHLDNLEDKEQMLILECVEQFSEASQQMAELSEVLVLRLEGYERQQKEITQLQAEITKLQQ
  RCQSYGAQTEKLQQQLASEKGVHPESLRAGSHMQDYGSRPRERQEDGKSHRQRSSMPAGSVTHYGYSVPL
  DALPSFPETLAEELRTSLRKFITDPAYFMERCDTRCREERKKEQGTMPPPPVQDLKPPEDFEAPEELVPE
  EELGAIEEVGTAEDGPAEETEQASEETEAWEEVEPEVDEATRMNVVVSALEASGLGPSHLDMKYVLQQLS
  NWQDAHSKRQQKQKVVPKDSPAPQQQTNMGGGIVEQQPIVPTQDSQRLEEDRATHSPSAREEEGPSGAT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_177982
• ORF Size: 1887 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_177982.1, NM_177982.2, NP_817091.1
• RefSeq Size: 3184
• RefSeq ORF: 1890
• Locus ID: 29430
• MW: 70.2 kDa
• Gene Summary: Huntington's disease (HD), a neurodegenerative disorder characterized by loss of striatal neurons, is caused by an expansion of a polyglutamine tract in the HD protein huntingtin. This gene encodes a protein that is homologous to the human huntingtin-associated protein 1. The human protein interacts with huntingtin, with two cytoskeletal proteins (dynactin and pericentriolar autoantigen protein 1), and with a hepatocyte growth factor-regulated tyrosine kinase substrate. The interactions with cytoskeletal proteins and a kinase substrate suggest a role for this protein in vesicular trafficking or organelle transport. Two transcripts encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]