Cast (NM_053295) Rat Tagged ORF Clone

CAT#: RR200416

Cast (Myc-DDK-tagged ORF) - Rat calpastatin (Cast), transcript variant 1, (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cast
• Synonyms: MGC108997
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR200416 representing NM_053295
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCCGGCCCGGCCCGAAGCCCGCCGCCTCTTCCAGGCCCCGGCGCGGGGCGGCAGCTAGCCACACCC
  AGGAGCATGTTAATGAAAAGAATATTGGATCATCTTCTAAACCAGCAGAAAAGAAAGGATCTGATGAGGT
  GACAGCTTCCTCTGCTGCCACTGGCACATCTCCCAGAATGAGTACCACAGGAGCTAAGGCTGTGAAAATA
  GAGTCTGAGAAGTCACAATCAAGTGAGCCACCTGTGATTCATGAGAAAAAGCCAAAAGGAAAGCCAAAGG
  AAGGGTCAGAGCCCCAAACTCTTCCTAAGCATGCATCAGATACAGGAAGTAAGCATGCTCACAAAGAAAA
  AGCACTTTCCAGATCAAATGAGCAGATAGTGTCAGAGAAATCATCAGAATCAAAGACAAAATTTCAAGAT
  GCTCCTTCTGCTGATGGAGAGAGTGTGGCTGGAGGTGTGACTGTGGCCACTGCCTCAGACAAGGTGGTTG
  TTAAGAAAAAAGAGAAGAAATCATTAACACCTACTTTACCTATGGAGTCCACACTAAACAAGCTATCTGA
  CAAGTCAGGCGTGAATGCTGCTTTGGATGACCTGATAGACACCTTAGGTGAATGTGAAGATACTAACAAA
  GATGATCCACCATATACTGGACCAGTAGTTCTGGACCCAATGGATTCTACCTACCTTGAGGCACTGGGTA
  TAAAAGAAGGGACTATTCCTCCAGAGTATAGGAAACTTCTGGAGAAAAATGAAGCTATCACAGGACCTCT
  TCCAGATTCTCCTAAACCTATGGGAATCGACCATGCTATAGATGCCTTGTCATCTGATTTCACCTGTAGC
  TCTCCAACTGGAAAGCAAACTGAGAAAGAGAAGTCTACTGGGGAAAGTTCGAAAGCTCAGTCTGCAGGAG
  TGACCAGAAGTGCTGTTCCTCCCCAGGAGAAGAAAAGGAAAGTAGAAGAGGAGGTCATGAATGATCAAGC
  CCTGCAGGCTCTGTCAGATTCACTGGGCACCCGGCAGCCAGACCCTCAGAGCCATCTTAGGCAAGCCAAA
  CAAGTCAAAGAGGCAAAAGCAAAAGAAGAAAGGCAGGAGAAATGTGGTGAAGATGAGGACACAGTACCTG
  CTGAGTACAGGTTAAAACCAGCCAAGGATAAAGATGGAAAACCACTATTGCCAGAACCTGAAGAAACATC
  TAAGTGCCTGAGTGAGTCAGAGCTGATTGGGGAGCTTTCAGCAGATTTTGTCCAACCTACATATCAAGAA
  AAACCATCTATGCCAGCTGCAAAAATAAAGAAGGGCGTAGTGCCAGATGATGCTGTAGAAACCTTGGCTA
  GAAGCCTGGGGACAAGGAAAGAAGATCCAGAAGATGAAAAATCTCTGGTGGATAAAGTCAAGGAGAAAGC
  TAAAGAAGAAGATCATGAAAAACTTGGTGAAAAAGAAGAAACAATTCCTCCTGATTATCGACTAGAAATA
  GTCAAGGATAAGGATGGAAAACCGCTCCTGCCAAAAGAAGCCGAGGAACAACTTCCACCCTTAAGCGATG
  ACTTCCTTCTTGATGCATTGTCTCAGGACTTCTCCAGTCCTGCAAACATATTGTCTCTTGGTTTTGAAGA
  TGCCAAACTTTCTGCTGCTGTTTCTGAAACAGTTTCCCAGGTGCCTGCTCCAAGCAACCACACAGCAGCC
  CCACCGCCTGGCACTGAGAGGAGAGACAAAGAACTCGATGATGCCTTGGATGAACTTTCTGACAGTCTTG
  GACAAAGACAGCCTGATCCAGATGAGAACAAACCACTAGATGATAAAGTAAAGGAAAAAATCAAAGCAGA
  GCATAGCGAGAAACTGGGAGAAAGAGACGACACCATCCCCCCTGAATATAGGCATCTCCTGGATAATGAT
  GGGAAGGACAAACCAGAGAAGCCACTGACAAAGAACACAGAGAAGCCTGGTCAGGACCAGGACCCCATTG
  ATGCCCTCTCAGAAGACTTGGATAGCTGCCCCCCAACTACAGAGACTTCACAGAATACAACAAAGGAGAA
  GGGTAAGAAGACTTCAAGTTCCAAAGCATCCAAGAATGAAGAGAAAACAAAGGATTCCTCCAAGAAGACA
  GAGGAAGTGCCCAAGCCAAAGGTGGATGAAGATGCAACA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR200416 representing NM_053295
  Red=Cloning site Green=Tags(s)
  
  MSRPGPKPAASSRPRRGAAASHTQEHVNEKNIGSSSKPAEKKGSDEVTASSAATGTSPRMSTTGAKAVKI
  ESEKSQSSEPPVIHEKKPKGKPKEGSEPQTLPKHASDTGSKHAHKEKALSRSNEQIVSEKSSESKTKFQD
  APSADGESVAGGVTVATASDKVVVKKKEKKSLTPTLPMESTLNKLSDKSGVNAALDDLIDTLGECEDTNK
  DDPPYTGPVVLDPMDSTYLEALGIKEGTIPPEYRKLLEKNEAITGPLPDSPKPMGIDHAIDALSSDFTCS
  SPTGKQTEKEKSTGESSKAQSAGVTRSAVPPQEKKRKVEEEVMNDQALQALSDSLGTRQPDPQSHLRQAK
  QVKEAKAKEERQEKCGEDEDTVPAEYRLKPAKDKDGKPLLPEPEETSKCLSESELIGELSADFVQPTYQE
  KPSMPAAKIKKGVVPDDAVETLARSLGTRKEDPEDEKSLVDKVKEKAKEEDHEKLGEKEETIPPDYRLEI
  VKDKDGKPLLPKEAEEQLPPLSDDFLLDALSQDFSSPANILSLGFEDAKLSAAVSETVSQVPAPSNHTAA
  PPPGTERRDKELDDALDELSDSLGQRQPDPDENKPLDDKVKEKIKAEHSEKLGERDDTIPPEYRHLLDND
  GKDKPEKPLTKNTEKPGQDQDPIDALSEDLDSCPPTTETSQNTTKEKGKKTSSSKASKNEEKTKDSSKKT
  EEVPKPKVDEDAT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_053295
• ORF Size: 2139 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_053295.2, NP_445747.2
• RefSeq Size: 4649
• RefSeq ORF: 2142
• Locus ID: 25403
• MW: 77.3 kDa
• Gene Summary: inhibitor of calpain; degradation is required for myoblast fusion; exists in multiple alternatively spliced isoforms [RGD, Feb 2006]