Adarb1 (NM_001111057) Rat Tagged ORF Clone

CAT#: RR200425

Adarb1 (Myc-DDK-tagged ORF) - Rat adenosine deaminase, RNA-specific, B1 (Adarb1), transcript variant 4, (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Adarb1
• Synonyms: Adar2; Red1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR200425 representing NM_001111057
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCCCCAAGGACAGCAGCACACCCGGGCCTGGCGAGGGTATTCCGCTCTCCAACGGGGGTGGTGGGA
  GCACCAGCAGGAAGCGGCCCCTGGAGGAGGGCAGCAATGGCCACTCCAAGTACCGCCTGAAGAAGCGAAG
  GAAAACGCCAGGGCCCGTTCTACCCAAGAACGCCCTGATGCAGCTGAACGAGATCAAACCGGGCTTACAG
  TACATGCTGCTGTCCCAGACAGGACCCGTGCACGCACCTCTGTTTGTCATGTCTGTGGAGGTAAACGGGC
  AGGTCTTTGAAGGCTCCGGCCCTACAAAGAAGAAGGCAAAGCTGCATGCTGCTGAGAAGGCCCTGCGGTC
  TTTTGTCCAGTTTCCCAACGCCTCTGAGGCCCACCTGGCCATGGGAAGGACCCTCTCCGTGAACACAGAC
  TTCACGTCCGACCAGGCGGACTTCCCCGACACGCTCTTCAATGGCTTTGAGACTCCAGACAAGTCGGAGC
  CACCCTTCTACGTAGGCTCCAATGGGGATGACTCCTTCAGCTCAAGCGGAGACGTTAGCCTGTCAGCCTC
  CCCAGTGCCTGCCAGCCTTACCCAGCCTCCTCTGCCCATCCCACCACCATTCCCACCCCCAAGTGGGAAG
  AACCCCGTGATGATCTTGAATGAGCTGCGCCCAGGGCTGAAGTATGACTTCCTCTCCGAGAGTGGGGAGA
  GCCACGCCAAGAGCTTTGTCATGTCCGTGGTGGTAGATGGCCAGTTCTTTGAGGGCTCAGGGAGAAACAA
  GAAGCTTGCCAAGGCCCGGGCTGCACAGTCTGCCTTGGCTACTGTCTTCAATTTGCACTTGGACCAAACG
  CCATCTCGCCAGCCTGTCCTCAGTGAGGGTCTCCAGTTGCATTTGCCACAGGTATTGGCAGATGCTGTCT
  CACGCCTGGTCCTGGGTAAGTTCAGTGACCTGACAGACAACTTTTCCTCCCCTCACGCACGAAGAAAAGT
  GCTCTCTGGAGTAGTGATGACCACAGGTACAGATGTCAAAGATGCCAAGGTGATAAGTGTTTCGACAGGG
  ACGAAGTGCATCAACGGCGAATACATGAGTGACCGTGGCCTGGCTCTCAATGACTGCCACGCAGAGATAA
  TCTCCCGAAGGTCCCTGCTCAGGTTTCTATACGCACAGCTCGAGCTTTACTTAAATAACAAAGAAGACCA
  GAAAAAGTCCATATTTCAGAAGTCAGAGCGGGGTGGGTTCCGGCTGAAGGATACCGTGCAGTTCCACCTG
  TACATCAGCACCTCACCCTGCGGAGACGCCAGAATATTCTCTCCCCATGAGCCCGTGCTAGAGGAACCGG
  CTGATAGACATCCGAATCGCAAAGCAAGGGGACAGCTGCGGACTAAAATAGAATCTGGCGAGGGGACAAT
  CCCTGTGCGCTCAAATGCCAGCATCCAGACCTGGGATGGGGTGCTGCAGGGGGAACGGCTGCTCACCATG
  TCCTGCAGTGACAAGATAGCACGCTGGAACGTGGTGGGCATCCAGGGGGCCCTGCTCAGCATTTTCGTGG
  AGCCCATCTACTTCTCCAGCATCATCCTGGGCAGCCTGTACCACGGGGACCACCTCTCCAGGGCCATGTA
  CCAGCGGATCTCCAACATAGAGGACCTGCCACCGCTCTACACCCTCAACAAGCCCCTGCTCAGCGGTATC
  AGCAATGCAGAGGCACGGCAGCCAGGGAAGGCACCCAACTTCAGTGTCAACTGGACGGTGGGCGACACGG
  CCATTGAGGTCATCAATGCCACAACAGGGAAGGATGAGCTAGGCCGCCCCTCCCGCCTGTGTAAGCACGC
  GCTGTACTGTCGCTGGATGCGGGTACACGGAAAGGTGCCCCCCCACCTGCTGCGCACCAAGATCACCAAG
  CCCACCACCTACCACGAGTCCAAGCTGGCAGCGAAGGAGTACCAGGCTGCCAAGGCACGTCTGTTCACTG
  CCTTCATCAAGGCGGGGCTGGGCGCCTGGGTGGAGAAGCCCACAGAGCAGGACCAGTTCTCCTTCACTCC
  C
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR200425 representing NM_001111057
  Red=Cloning site Green=Tags(s)
  
  MPPKDSSTPGPGEGIPLSNGGGGSTSRKRPLEEGSNGHSKYRLKKRRKTPGPVLPKNALMQLNEIKPGLQ
  YMLLSQTGPVHAPLFVMSVEVNGQVFEGSGPTKKKAKLHAAEKALRSFVQFPNASEAHLAMGRTLSVNTD
  FTSDQADFPDTLFNGFETPDKSEPPFYVGSNGDDSFSSSGDVSLSASPVPASLTQPPLPIPPPFPPPSGK
  NPVMILNELRPGLKYDFLSESGESHAKSFVMSVVVDGQFFEGSGRNKKLAKARAAQSALATVFNLHLDQT
  PSRQPVLSEGLQLHLPQVLADAVSRLVLGKFSDLTDNFSSPHARRKVLSGVVMTTGTDVKDAKVISVSTG
  TKCINGEYMSDRGLALNDCHAEIISRRSLLRFLYAQLELYLNNKEDQKKSIFQKSERGGFRLKDTVQFHL
  YISTSPCGDARIFSPHEPVLEEPADRHPNRKARGQLRTKIESGEGTIPVRSNASIQTWDGVLQGERLLTM
  SCSDKIARWNVVGIQGALLSIFVEPIYFSSIILGSLYHGDHLSRAMYQRISNIEDLPPLYTLNKPLLSGI
  SNAEARQPGKAPNFSVNWTVGDTAIEVINATTGKDELGRPSRLCKHALYCRWMRVHGKVPPHLLRTKITK
  PTTYHESKLAAKEYQAAKARLFTAFIKAGLGAWVEKPTEQDQFSFTP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001111057
• ORF Size: 2031 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001111057.1, NP_001104527.1
• RefSeq Size: 6429
• RefSeq ORF: 2034
• Locus ID: 25367
• MW: 74.1 kDa
• Gene Summary: This gene encodes a double-stranded-RNA-specific adenosine deaminase that is involved in editing pre-mRNAs by site-specific conversion of adenosine (A) to inosine (I). Substrates for this enzyme include ionotropic glutamate receptors (GluR2-6) and serotonin receptor (5HT2C). Studies in rodents have shown that this protein can modify its own pre-mRNA by A->I editing to create a novel acceptor splice site, alternative splicing to which results in down regulation of its protein expression. Additional splicing events result in transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]