Asic2 (NM_012892) Rat Tagged ORF Clone

CAT#: RR200429

Asic2 (Myc-DDK-tagged ORF) - Rat amiloride-sensitive cation channel 1, neuronal (Accn1), transcript variant 1, (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Asic2
• Synonyms: Accn1; BNaC1; BNC1k; MDEG; MDEG1; MDEG2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR200429 representing NM_012892
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCCGGAGCGGCGGAGCCCGGCTGCCCGCGACCGCGCTCAGCGGCCCGGGACGCTTCCGTATGGCCC
  GCGAGCAGCCGGCGCCCGTGGCGGTGGCGGCAGCTAGGCAGCCCGGAGGAGACCGGAGCGGCGATCCGGC
  GCTGCAGGGGCCAGGGGTCGCCCGCAGGGGGCGGCCGTCCCTGAGTCGCACTAAATTGCACGGGCTGCGG
  CACATGTGCGCGGGGCGCACGGCGGCGGGAGGCTCTTTCCAGCGACGGGCGCTGTGGGTGCTGGCCTTCT
  GCACGTCCCTCGGCTTGCTGCTGTCCTGGTCCTCGAACCGCCTGCTCTACTGGCTCAGCTTCCCGTCACA
  CACACGAGTGCACCGTGAGTGGAGCCGCCAGCTGCCGTTCCCCGCCGTCACCGTGTGCAACAACAACCCC
  CTGCGCTTCCCGCGCCTCTCCAAGGGGGACCTCTACTACGCGGGCCACTGGCTAGGGCTGCTGCTTCCCA
  ACCGCACCGCGCGCCCGCTGGTCAGCGAGCTGCTGCGGGGCGACGAGCCGCGCCGCCAGTGGTTCCGCAA
  ACTGGCCGACTTCCGCCTCTTCCTGCCGCCGCGCCACTTCGAGGGCATCAGCGCTGCCTTCATGGACCGT
  TTGGGCCACCAGCTGGAGGATATGCTGCTCTCCTGCAAGTACCGGGGCGAGCTCTGTGGCCCGCACAACT
  TCTCCTCAGTGTTTACAAAATACGGGAAGTGTTACATGTTTAACTCAGGCGAGGATGGCAAGCCGCTGCT
  CACCACGGTCAAGGGGGGGACGGGCAACGGGCTGGAGATCATGCTGGACATTCAGCAAGATGAGTACCTG
  CCCATCTGGGGAGAGACAGAGGAAACAACGTTTGAAGCAGGAGTGAAGGTTCAGATCCACAGTCAGTCTG
  AGCCGCCTTTCATCCAAGAGCTGGGCTTTGGGGTGGCTCCGGGGTTCCAGACCTTCGTGGCCACACAAGA
  GCAGAGGCTCACATATCTGCCCCCACCATGGGGGGAGTGCCGGTCCTCAGAGATGGGACTCGACTTCTTT
  CCTGTTTACAGCATCACAGCCTGTCGGATTGACTGTGAGACCCGCTACATCGTGGAGAACTGTAACTGCC
  GCATGGTCCACATGCCAGGGGACGCCCCTTTCTGCACCCCTGAGCAGCACAAGGAGTGTGCAGAGCCTGC
  CCTCGGTCTACTGGCAGAAAAGGACAGCAATTACTGTCTCTGCAGGACACCCTGCAACCTGACACGCTAC
  AACAAAGAGCTCTCCATGGTGAAGATCCCCAGCAAGACGTCAGCCAAGTACTTAGAGAAGAAATTTAACA
  AATCGGAAAAATATATCTCAGAGAACATTCTTGTTCTGGACATATTTTTTGAGGCGCTCAATTACGAAAC
  AATTGAACAGAAGAAGGCGTATGAAGTTGCTGCCTTACTTGGTGACATCGGTGGTCAGATGGGACTGTTC
  ATTGGTGCTAGTCTCCTCACAATACTAGAGCTCTTTGATTATATTTATGAGCTGATCAAAGAGAAGCTAT
  TAGACCTGCTTGGCAAAGAAGAAGAGGAAGGGAGCCACGATGAGAACATGAGCACCTGTGACACAATGCC
  AAACCACTCTGAAACCATCAGCCACACTGTGAACGTGCCCCTGCAGACAGCTTTGGGCACCCTGGAGGAG
  ATTGCCTGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR200429 representing NM_012892
  Red=Cloning site Green=Tags(s)
  
  MSRSGGARLPATALSGPGRFRMAREQPAPVAVAAARQPGGDRSGDPALQGPGVARRGRPSLSRTKLHGLR
  HMCAGRTAAGGSFQRRALWVLAFCTSLGLLLSWSSNRLLYWLSFPSHTRVHREWSRQLPFPAVTVCNNNP
  LRFPRLSKGDLYYAGHWLGLLLPNRTARPLVSELLRGDEPRRQWFRKLADFRLFLPPRHFEGISAAFMDR
  LGHQLEDMLLSCKYRGELCGPHNFSSVFTKYGKCYMFNSGEDGKPLLTTVKGGTGNGLEIMLDIQQDEYL
  PIWGETEETTFEAGVKVQIHSQSEPPFIQELGFGVAPGFQTFVATQEQRLTYLPPPWGECRSSEMGLDFF
  PVYSITACRIDCETRYIVENCNCRMVHMPGDAPFCTPEQHKECAEPALGLLAEKDSNYCLCRTPCNLTRY
  NKELSMVKIPSKTSAKYLEKKFNKSEKYISENILVLDIFFEALNYETIEQKKAYEVAALLGDIGGQMGLF
  IGASLLTILELFDYIYELIKEKLLDLLGKEEEEGSHDENMSTCDTMPNHSETISHTVNVPLQTALGTLEE
  IAC
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_012892
• ORF Size: 1689 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_012892.2, NP_037024.2
• RefSeq Size: 1948
• RefSeq ORF: 1692
• Locus ID: 25364
• MW: 63.1 kDa
• Gene Summary: member of the degenerin/epithelial Na(+) channel superfamily; permeable for Na(+), K(+) and Li(+); mutations may lead to a form of hereditary neurodegeneration; may be important for pain sensation [RGD, Feb 2006]