Prlr (NM_001034111) Rat Tagged ORF Clone

CAT#: RR200491

Prlr (Myc-DDK-tagged ORF) - Rat prolactin receptor (Prlr), transcript variant 1, (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Prlr
• Synonyms: RATPRLR
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR200491 representing NM_001034111
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCATCTGCACTTGCTTTCGTCCTACTTGTTCTCAACATCAGCCTCCTGAAGGGACAGTCACCACCAG
  GGAAACCTGAGATCCACAAATGTCGCTCTCCTGACAAGGAAACATTCACCTGCTGGTGGAATCCTGGGAC
  AGATGGAGGACTTCCTACCAATTATTCACTGACTTACAGCAAAGAAGGAGAGAAAACCACCTACGAATGT
  CCAGACTACAAAACCAGTGGCCCCAACTCCTGCTTCTTTAGCAAGCAGTACACTTCCATCTGGAAAATAT
  ATATCATCACAGTAAATGCCACGAACCAAATGGGAAGCAGTTCCTCGGATCCACTTTATGTGGATGTGAC
  TTACATCGTTGAGCCAGAGCCTCCTCGGAACCTGACATTAGAAGTAAAACAGCTAAAAGACAAAAAAACA
  TATCTGTGGGTAAAATGGTCCCCACCCACCATAACTGATGTGAAAACTGGTTGGTTTACAATGGAATATG
  AAATTCGATTAAAGCCTGAAGAAGCAGAAGAGTGGGAGATCCATTTTACAGGTCATCAAACACAGTTTAA
  AGTTTTTGACCTATATCCAGGGCAAAAGTATCTTGTCCAGACTCGCTGCAAGCCAGACCATGGATACTGG
  AGTAGATGGAGCCAGGAGAGTTCCGTTGAAATGCCAAATGACTTCACCTTGAAGGACACAACCGTGTGGA
  TCATTGTGGCCATTCTCTCTGCTGTCATCTGTTTGATTATGGTCTGGGCAGTGGCTTTGAAGGGCTATAG
  CATGATGACCTGCATCTTTCCACCAGTTCCTGGGCCAAAAATAAAAGGATTTGATACCCATCTGCTGGAG
  AAGGGCAAGTCTGAAGAGCTGCTGAGTGCCTTGGGGTGCCAAGACTTTCCCCCTACTTCTGACTGTGAGG
  ACTTGCTGGTGGAGTTCTTAGAAGTTGATGACAATGAGGACGAGCGGCTAATGCCATCCCATTCCAAAGA
  GTATCCAGGTCAAGGTGTTAAGCCCACACACCTAGATCCCGACAGTGACTCTGGTCACGGAAGCTATGAC
  AGCCATTCTCTTTTATCTGAAAAGTGTGAGGAACCCCAGGCCTACCCCCCTACTTTGCACATCCCTGAGA
  TCACTGAGAAGCCAGAGAATCCTGAAGCAAATATTCCTCCCACCGTGGACCCCCAAAGCACCAACCCCAA
  TTTTCATGTAGATGCACCCAAATCTTCAACATGGCCATTACTGCCTGGCCAACACATGCCCAGATCTCCT
  TACCACAGTGTTGCTGATGTGTGCAAGCTAGCCGGAAGTCCTGTGAATACACTGGACTCTTTCTTGGACA
  AAGCAGAGGAAAATGTTCTAAAGTTGTCTAAAGCCCTTGAGACTGGAGAGGAAGAAGTGGCTAAGCAAAA
  AGGGGCAAAAAGCTTCCCTTCTGACAAACAAAACACACCTTGGCCGCTGCTCCAGGAGAAAAGCCCCACT
  GTCTATGTTAAACCCCCAGATTATGTGGAGATTCACAAAGTCAACAAAGATGGAGTGCTATCATTATTCC
  CCAAGCAGAGAGAAAACAACCAGACAGAGAAGCCTGGGGTTCCTGAAACCAGTAAGGAGTATGCCAAGGT
  GTCTGGCATTATGGATAACAATATCCTCGTATTAGTGCCAGACTCACGAGCCCAGAACACAGCGTTGCTC
  GAGGAATCAGCCAAGAAGGCTCCACCATCGTTTGAAGCTGACCAATCTGAGAAAGATCTGGCCAGCTTCA
  CTGCAACCTCAAGCAACCGCAGACTCCAACTGGGTAGGCTGGATTACCTGGATCCTACGTGCTTCATGCA
  CTCCTTTCAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR200491 representing NM_001034111
  Red=Cloning site Green=Tags(s)
  
  MPSALAFVLLVLNISLLKGQSPPGKPEIHKCRSPDKETFTCWWNPGTDGGLPTNYSLTYSKEGEKTTYEC
  PDYKTSGPNSCFFSKQYTSIWKIYIITVNATNQMGSSSSDPLYVDVTYIVEPEPPRNLTLEVKQLKDKKT
  YLWVKWSPPTITDVKTGWFTMEYEIRLKPEEAEEWEIHFTGHQTQFKVFDLYPGQKYLVQTRCKPDHGYW
  SRWSQESSVEMPNDFTLKDTTVWIIVAILSAVICLIMVWAVALKGYSMMTCIFPPVPGPKIKGFDTHLLE
  KGKSEELLSALGCQDFPPTSDCEDLLVEFLEVDDNEDERLMPSHSKEYPGQGVKPTHLDPDSDSGHGSYD
  SHSLLSEKCEEPQAYPPTLHIPEITEKPENPEANIPPTVDPQSTNPNFHVDAPKSSTWPLLPGQHMPRSP
  YHSVADVCKLAGSPVNTLDSFLDKAEENVLKLSKALETGEEEVAKQKGAKSFPSDKQNTPWPLLQEKSPT
  VYVKPPDYVEIHKVNKDGVLSLFPKQRENNQTEKPGVPETSKEYAKVSGIMDNNILVLVPDSRAQNTALL
  EESAKKAPPSFEADQSEKDLASFTATSSNRRLQLGRLDYLDPTCFMHSFH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001034111
• ORF Size: 1830 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001034111.1, NP_001029283.1
• RefSeq Size: 2132 bp
• RefSeq ORF: 1833 bp
• Locus ID: 24684
• Cytogenetics: 2q16
• MW: 68.6 kDa
• Gene Summary: This is a receptor for the anterior pituitary hormone prolactin. [UniProtKB/Swiss-Prot Function]