Pde4d (NM_017032) Rat Tagged ORF Clone

CAT#: RR200497

Pde4d (Myc-DDK-tagged ORF) - Rat phosphodiesterase 4D, cAMP-specific (phosphodiesterase E3 dunce homolog, Drosophila) (Pde4d), transcript variant 5, (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Pde4d
• Synonyms: DPDE3; PDE3/IVd
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR200497 representing NM_017032
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGCATGTGAATACCTTCCCTTTCAGAAGGCATTCCTGGATATGTTTCGATGTGGACAATGGCACAT
  CAGCGGGACGGAGTCCCTTGGATCCCATGACCAGCCCAGGGTCTGGGCTAATTCTCCAAGCAAACTTTGT
  GCACAGTCAACGCCGGGAGTCCTTCCTGTATCGATCAGACAGCGATTATGACCTCTCTCCAAAGTCTATG
  TCCAGGAACTCCTCGATTGCCAGTGATATACATGGAGATGACTTGATTGTGACTCCATTTGCTCAGGTCT
  TGGCCAGCCTTCGAACTGTAAGAAACAACTTTGCTGCATTAACTAATTTGCAAGATCGCGCACCCAGCAA
  AAGATCACCCATGTGCAACCAACCATCCATCAACAAAGCCACCATCACAGAGGAGGCCTACCAGAAACTG
  GCCAGCGAGACCCTGGAGGAGCTGGACTGGTGTCTGGACCAGCTAGAGACCCTGCAGACCAGGCACTCCG
  TCAGTGAGATGGCCTCCAACAAGTTCAAGAGGATGCTTAATCGGGAGCTCACCCACCTCTCTGAAATGAG
  TCGGTCTGGCAACCAGGTGTCGGAGTACATATCAAACACGTTCTTAGATAAGCAACATGAAGTAGAAATT
  CCCTCTCCGACTCAGAAGGAAAAAGAGAAGAAGAAAAGGCCGATGTCACAGATCAGTGGGGTCAAGAAGT
  TGATGCACAGCTCCAGCCTGACCAATTCCTGCATTCCAAGATTTGGGGTTAAAACAGAGCAGGAAGATGT
  CCTGGCCAAGGAACTAGAAGACGTGAACAAGTGGGGCCTCCACGTTTTCCGAATAGCGGAGCTGTCTGGC
  AACCGGCCTCTGACTGTTATCATGCACACCATTTTTCAGGAACGAGATTTGTTAAAAACGTTTAAAATCC
  CAGTGGACACTTTGATTACGTATCTTATGACTCTAGAAGACCATTACCATGCTGACGTGGCCTATCACAA
  CAACATCCATGCTGCAGATGTCGTCCAGTCAACTCATGTGCTGCTCTCTACACCCGCTTTGGAGGCTGTT
  TTCACTGACTTGGAGATTCTCGCGGCCATTTTTGCCAGTGCAATACATGATGTGGATCATCCTGGTGTGT
  CAAATCAATTTCTGATCAATACAAACTCGGAACTTGCCTTGATGTACAACGACTCCTCCGTCTTAGAGAA
  TCATCATTTGGCTGTGGGCTTTAAGTTGCTCCAGGAAGAAAACTGTGACATTTTCCAGAATCTGACCAAA
  AAGCAAAGACAATCTTTAAGGAAAATGGCCATTGACATTGTACTAGCGACAGACATGTCAAAGCACATGA
  ATCTGCTGGCTGATCTGAAAACAATGGTTGAAACGAAGAAGGTGACGAGCTCTGGCGTCCTCCTCCTTGA
  TAACTATTCTGACAGGATCCAGGTCCTCCAGAATATGGTGCACTGTGCAGACCTGAGCAACCCCACAAAG
  CCACTCCAGCTCTACCGCCAGTGGACGGACCGGATAATGGAGGAGTTCTTCCGTCAGGGGGACCGGGAGC
  GTGAGCGTGGCATGGAGATAAGTCCCATGTGTGACAAGCACAACGCCTCTGTGGAAAAATCACAGGTGGG
  CTTCATAGACTACATTGTTCATCCACTCTGGGAGACATGGGCAGACCTCGTACATCCCGACGCCCAGGAC
  ATTTTGGACACTTTGGAGGACAATCGTGAGTGGTACCAGAGCACAATCCCCCAGAGCCCCTCCCCTGCAC
  CTGATGACCAAGAGGACGGCCGTCAGGGACAGACTGAAAAATTCCAGTTCGAACTAACCTTAGAGGAAGA
  TGGCGAGTCAGACACTGAAAAGGACAGTGGAAGTCAAGTGGAGGAAGACACTAGCTGCAGTGACTCTAAG
  ACTCTGTGCACCCAAGACTCAGAGTCCACTGAAATTCCCCTTGACGAGCAGGTTGAAGAGGAGGCTGTAG
  CAGAAGAGGAAAGCCAGCCCCAAACTGGCGTCGCAGACGATTGTTGTCCTGACACG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR200497 representing NM_017032
  Red=Cloning site Green=Tags(s)
  
  MMHVNTFPFRRHSWICFDVDNGTSAGRSPLDPMTSPGSGLILQANFVHSQRRESFLYRSDSDYDLSPKSM
  SRNSSIASDIHGDDLIVTPFAQVLASLRTVRNNFAALTNLQDRAPSKRSPMCNQPSINKATITEEAYQKL
  ASETLEELDWCLDQLETLQTRHSVSEMASNKFKRMLNRELTHLSEMSRSGNQVSEYISNTFLDKQHEVEI
  PSPTQKEKEKKKRPMSQISGVKKLMHSSSLTNSCIPRFGVKTEQEDVLAKELEDVNKWGLHVFRIAELSG
  NRPLTVIMHTIFQERDLLKTFKIPVDTLITYLMTLEDHYHADVAYHNNIHAADVVQSTHVLLSTPALEAV
  FTDLEILAAIFASAIHDVDHPGVSNQFLINTNSELALMYNDSSVLENHHLAVGFKLLQEENCDIFQNLTK
  KQRQSLRKMAIDIVLATDMSKHMNLLADLKTMVETKKVTSSGVLLLDNYSDRIQVLQNMVHCADLSNPTK
  PLQLYRQWTDRIMEEFFRQGDRERERGMEISPMCDKHNASVEKSQVGFIDYIVHPLWETWADLVHPDAQD
  ILDTLEDNREWYQSTIPQSPSPAPDDQEDGRQGQTEKFQFELTLEEDGESDTEKDSGSQVEEDTSCSDSK
  TLCTQDSESTEIPLDEQVEEEAVAEEESQPQTGVADDCCPDT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_017032
• ORF Size: 2016 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_017032.1, NP_058728.1
• RefSeq Size: 2077
• RefSeq ORF: 2019
• Locus ID: 24627
• MW: 76.3 kDa
• Gene Summary: may contain eight isoforms that display differential expression and activities; isorofms Pde4D6 and Pde4D7 exhibit high affinity for cyclic AMP and are inhibited by rolipram [RGD, Feb 2006]