Pde4d (NM_001113334) Rat Tagged ORF Clone

CAT#: RR200498

Pde4d (Myc-DDK-tagged ORF) - Rat phosphodiesterase 4D, cAMP-specific (phosphodiesterase E3 dunce homolog, Drosophila) (Pde4d), transcript variant 4, (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Pde4d
• Synonyms: DPDE3; PDE3/IVd
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR200498 representing NM_001113334
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTTTCGTTTGGGATCCTCTGGGAGTCACAGTGCCAGGTCCATCTCCAAGAACTAGGACAAGATTGC
  GTTTCTCAAAGTCCTACAGTTTCGATGTGGACAATGGCACATCAGCGGGACGGAGTCCCTTGGATCCCAT
  GACCAGCCCAGGGTCTGGGCTAATTCTCCAAGCAAACTTTGTGCACAGTCAACGCCGGGAGTCCTTCCTG
  TATCGATCAGACAGCGATTATGACCTCTCTCCAAAGTCTATGTCCAGGAACTCCTCGATTGCCAGTGATA
  TACATGGAGATGACTTGATTGTGACTCCATTTGCTCAGGTCTTGGCCAGCCTTCGAACTGTAAGAAACAA
  CTTTGCTGCATTAACTAATTTGCAAGATCGCGCACCCAGCAAAAGATCACCCATGTGCAACCAACCATCC
  ATCAACAAAGCCACCATCACAGAGGAGGCCTACCAGAAACTGGCCAGCGAGACCCTGGAGGAGCTGGACT
  GGTGTCTGGACCAGCTAGAGACCCTGCAGACCAGGCACTCCGTCAGTGAGATGGCCTCCAACAAGTTCAA
  GAGGATGCTTAATCGGGAGCTCACCCACCTCTCTGAAATGAGTCGGTCTGGCAACCAGGTGTCGGAGTAC
  ATATCAAACACGTTCTTAGATAAGCAACATGAAGTAGAAATTCCCTCTCCGACTCAGAAGGAAAAAGAGA
  AGAAGAAAAGGCCGATGTCACAGATCAGTGGGGTCAAGAAGTTGATGCACAGCTCCAGCCTGACCAATTC
  CTGCATTCCAAGATTTGGGGTTAAAACAGAGCAGGAAGATGTCCTGGCCAAGGAACTAGAAGACGTGAAC
  AAGTGGGGCCTCCACGTTTTCCGAATAGCGGAGCTGTCTGGCAACCGGCCTCTGACTGTTATCATGCACA
  CCATTTTTCAGGAACGAGATTTGTTAAAAACGTTTAAAATCCCAGTGGACACTTTGATTACGTATCTTAT
  GACTCTAGAAGACCATTACCATGCTGACGTGGCCTATCACAACAACATCCATGCTGCAGATGTCGTCCAG
  TCAACTCATGTGCTGCTCTCTACACCCGCTTTGGAGGCTGTTTTCACTGACTTGGAGATTCTCGCGGCCA
  TTTTTGCCAGTGCAATACATGATGTGGATCATCCTGGTGTGTCAAATCAATTTCTGATCAATACAAACTC
  GGAACTTGCCTTGATGTACAACGACTCCTCCGTCTTAGAGAATCATCATTTGGCTGTGGGCTTTAAGTTG
  CTCCAGGAAGAAAACTGTGACATTTTCCAGAATCTGACCAAAAAGCAAAGACAATCTTTAAGGAAAATGG
  CCATTGACATTGTACTAGCGACAGACATGTCAAAGCACATGAATCTGCTGGCTGATCTGAAAACAATGGT
  TGAAACGAAGAAGGTGACGAGCTCTGGCGTCCTCCTCCTTGATAACTATTCTGACAGGATCCAGGTCCTC
  CAGAATATGGTGCACTGTGCAGACCTGAGCAACCCCACAAAGCCACTCCAGCTCTACCGCCAGTGGACGG
  ACCGGATAATGGAGGAGTTCTTCCGTCAGGGGGACCGGGAGCGTGAGCGTGGCATGGAGATAAGTCCCAT
  GTGTGACAAGCACAACGCCTCTGTGGAAAAATCACAGGTGGGCTTCATAGACTACATTGTTCATCCACTC
  TGGGAGACATGGGCAGACCTCGTACATCCCGACGCCCAGGACATTTTGGACACTTTGGAGGACAATCGTG
  AGTGGTACCAGAGCACAATCCCCCAGAGCCCCTCCCCTGCACCTGATGACCAAGAGGACGGCCGTCAGGG
  ACAGACTGAAAAATTCCAGTTCGAACTAACCTTAGAGGAAGATGGCGAGTCAGACACTGAAAAGGACAGT
  GGAAGTCAAGTGGAGGAAGACACTAGCTGCAGTGACTCTAAGACTCTGTGCACCCAAGACTCAGAGTCCA
  CTGAAATTCCCCTTGACGAGCAGGTTGAAGAGGAGGCTGTAGCAGAAGAGGAAAGCCAGCCCCAAACTGG
  CGTCGCAGACGATTGTTGTCCTGACACG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR200498 representing NM_001113334
  Red=Cloning site Green=Tags(s)
  
  MAFVWDPLGVTVPGPSPRTRTRLRFSKSYSFDVDNGTSAGRSPLDPMTSPGSGLILQANFVHSQRRESFL
  YRSDSDYDLSPKSMSRNSSIASDIHGDDLIVTPFAQVLASLRTVRNNFAALTNLQDRAPSKRSPMCNQPS
  INKATITEEAYQKLASETLEELDWCLDQLETLQTRHSVSEMASNKFKRMLNRELTHLSEMSRSGNQVSEY
  ISNTFLDKQHEVEIPSPTQKEKEKKKRPMSQISGVKKLMHSSSLTNSCIPRFGVKTEQEDVLAKELEDVN
  KWGLHVFRIAELSGNRPLTVIMHTIFQERDLLKTFKIPVDTLITYLMTLEDHYHADVAYHNNIHAADVVQ
  STHVLLSTPALEAVFTDLEILAAIFASAIHDVDHPGVSNQFLINTNSELALMYNDSSVLENHHLAVGFKL
  LQEENCDIFQNLTKKQRQSLRKMAIDIVLATDMSKHMNLLADLKTMVETKKVTSSGVLLLDNYSDRIQVL
  QNMVHCADLSNPTKPLQLYRQWTDRIMEEFFRQGDRERERGMEISPMCDKHNASVEKSQVGFIDYIVHPL
  WETWADLVHPDAQDILDTLEDNREWYQSTIPQSPSPAPDDQEDGRQGQTEKFQFELTLEEDGESDTEKDS
  GSQVEEDTSCSDSKTLCTQDSESTEIPLDEQVEEEAVAEEESQPQTGVADDCCPDT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001113334
• ORF Size: 2058 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001113334.1, NP_001106805.1
• RefSeq Size: 2159
• RefSeq ORF: 2061
• Locus ID: 24627
• MW: 77.6 kDa
• Gene Summary: may contain eight isoforms that display differential expression and activities; isorofms Pde4D6 and Pde4D7 exhibit high affinity for cyclic AMP and are inhibited by rolipram [RGD, Feb 2006]