Pde4d (NM_001113328) Rat Tagged ORF Clone

CAT#: RR200499

Pde4d (Myc-DDK-tagged ORF) - Rat phosphodiesterase 4D, cAMP-specific (phosphodiesterase E3 dunce homolog, Drosophila) (Pde4d), transcript variant 1, (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Pde4d
• Synonyms: DPDE3; PDE3/IVd
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR200499 representing NM_001113328
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGCAGAGGGCAGCAGCGTGCCGGCCCGGGCGGGCAGCGACGAGGGCAGCGACAGCTCTGGAGGGG
  CCGCGCTCAAAGCCCCCAAGCATCTCTGGAGGCACGAGCAGCACCACCAGTACCCGCTCCGGCAGCCCCA
  GTTCCGCCTCTTGCACCCCCATCACCACCTGCCCCCGCCGCCGCCACCCTCGCCCCAGCCCCAGCTGCAG
  CCACCACCACCGCCCCCACTGCCACCACCCCCGCCACCGCCCGGAGCTACCCGTGGTCGCTACGCCTCAA
  GCGGGGCTAGCCGCGTCCGGCACCGCGGCTACTCGGACACGGAGCGCTACCTGTACTGCCGTGCCATGGA
  CCGCACCTCCTACGCGGTGGAGACTGGTCACCGGCCAGGCCTGAAGAAATCTAGGATGTCCTGGCCCTCG
  TCTTTCCAGGGACTCAGACGTTTCGATGTGGACAATGGCACATCAGCGGGACGGAGTCCCTTGGATCCCA
  TGACCAGCCCAGGGTCTGGGCTAATTCTCCAAGCAAACTTTGTGCACAGTCAACGCCGGGAGTCCTTCCT
  GTATCGATCAGACAGCGATTATGACCTCTCTCCAAAGTCTATGTCCAGGAACTCCTCGATTGCCAGTGAT
  ATACATGGAGATGACTTGATTGTGACTCCATTTGCTCAGGTCTTGGCCAGCCTTCGAACTGTAAGAAACA
  ACTTTGCTGCATTAACTAATTTGCAAGATCGCGCACCCAGCAAAAGATCACCCATGTGCAACCAACCATC
  CATCAACAAAGCCACCATCACAGAGGAGGCCTACCAGAAACTGGCCAGCGAGACCCTGGAGGAGCTGGAC
  TGGTGTCTGGACCAGCTAGAGACCCTGCAGACCAGGCACTCCGTCAGTGAGATGGCCTCCAACAAGTTCA
  AGAGGATGCTTAATCGGGAGCTCACCCACCTCTCTGAAATGAGTCGGTCTGGCAACCAGGTGTCGGAGTA
  CATATCAAACACGTTCTTAGATAAGCAACATGAAGTAGAAATTCCCTCTCCGACTCAGAAGGAAAAAGAG
  AAGAAGAAAAGGCCGATGTCACAGATCAGTGGGGTCAAGAAGTTGATGCACAGCTCCAGCCTGACCAATT
  CCTGCATTCCAAGATTTGGGGTTAAAACAGAGCAGGAAGATGTCCTGGCCAAGGAACTAGAAGACGTGAA
  CAAGTGGGGCCTCCACGTTTTCCGAATAGCGGAGCTGTCTGGCAACCGGCCTCTGACTGTTATCATGCAC
  ACCATTTTTCAGGAACGAGATTTGTTAAAAACGTTTAAAATCCCAGTGGACACTTTGATTACGTATCTTA
  TGACTCTAGAAGACCATTACCATGCTGACGTGGCCTATCACAACAACATCCATGCTGCAGATGTCGTCCA
  GTCAACTCATGTGCTGCTCTCTACACCCGCTTTGGAGGCTGTTTTCACTGACTTGGAGATTCTCGCGGCC
  ATTTTTGCCAGTGCAATACATGATGTGGATCATCCTGGTGTGTCAAATCAATTTCTGATCAATACAAACT
  CGGAACTTGCCTTGATGTACAACGACTCCTCCGTCTTAGAGAATCATCATTTGGCTGTGGGCTTTAAGTT
  GCTCCAGGAAGAAAACTGTGACATTTTCCAGAATCTGACCAAAAAGCAAAGACAATCTTTAAGGAAAATG
  GCCATTGACATTGTACTAGCGACAGACATGTCAAAGCACATGAATCTGCTGGCTGATCTGAAAACAATGG
  TTGAAACGAAGAAGGTGACGAGCTCTGGCGTCCTCCTCCTTGATAACTATTCTGACAGGATCCAGGTCCT
  CCAGAATATGGTGCACTGTGCAGACCTGAGCAACCCCACAAAGCCACTCCAGCTCTACCGCCAGTGGACG
  GACCGGATAATGGAGGAGTTCTTCCGTCAGGGGGACCGGGAGCGTGAGCGTGGCATGGAGATAAGTCCCA
  TGTGTGACAAGCACAACGCCTCTGTGGAAAAATCACAGGTGGGCTTCATAGACTACATTGTTCATCCACT
  CTGGGAGACATGGGCAGACCTCGTACATCCCGACGCCCAGGACATTTTGGACACTTTGGAGGACAATCGT
  GAGTGGTACCAGAGCACAATCCCCCAGAGCCCCTCCCCTGCACCTGATGACCAAGAGGACGGCCGTCAGG
  GACAGACTGAAAAATTCCAGTTCGAACTAACCTTAGAGGAAGATGGCGAGTCAGACACTGAAAAGGACAG
  TGGAAGTCAAGTGGAGGAAGACACTAGCTGCAGTGACTCTAAGACTCTGTGCACCCAAGACTCAGAGTCC
  ACTGAAATTCCCCTTGACGAGCAGGTTGAAGAGGAGGCTGTAGCAGAAGAGGAAAGCCAGCCCCAAACTG
  GCGTCGCAGACGATTGTTGTCCTGACACGGCGTCGCAGACGATTGTTGTCCTGACACG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR200499 representing NM_001113328
  Red=Cloning site Green=Tags(s)
  
  MEAEGSSVPARAGSDEGSDSSGGAALKAPKHLWRHEQHHQYPLRQPQFRLLHPHHHLPPPPPPSPQPQLQ
  PPPPPPLPPPPPPPGATRGRYASSGASRVRHRGYSDTERYLYCRAMDRTSYAVETGHRPGLKKSRMSWPS
  SFQGLRRFDVDNGTSAGRSPLDPMTSPGSGLILQANFVHSQRRESFLYRSDSDYDLSPKSMSRNSSIASD
  IHGDDLIVTPFAQVLASLRTVRNNFAALTNLQDRAPSKRSPMCNQPSINKATITEEAYQKLASETLEELD
  WCLDQLETLQTRHSVSEMASNKFKRMLNRELTHLSEMSRSGNQVSEYISNTFLDKQHEVEIPSPTQKEKE
  KKKRPMSQISGVKKLMHSSSLTNSCIPRFGVKTEQEDVLAKELEDVNKWGLHVFRIAELSGNRPLTVIMH
  TIFQERDLLKTFKIPVDTLITYLMTLEDHYHADVAYHNNIHAADVVQSTHVLLSTPALEAVFTDLEILAA
  IFASAIHDVDHPGVSNQFLINTNSELALMYNDSSVLENHHLAVGFKLLQEENCDIFQNLTKKQRQSLRKM
  AIDIVLATDMSKHMNLLADLKTMVETKKVTSSGVLLLDNYSDRIQVLQNMVHCADLSNPTKPLQLYRQWT
  DRIMEEFFRQGDRERERGMEISPMCDKHNASVEKSQVGFIDYIVHPLWETWADLVHPDAQDILDTLEDNR
  EWYQSTIPQSPSPAPDDQEDGRQGQTEKFQFELTLEEDGESDTEKDSGSQVEEDTSCSDSKTLCTQDSES
  TEIPLDEQVEEEAVAEEESQPQTGVADDCCPDTASQTIVVLT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001113328
• ORF Size: 2409 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq Size: 2470
• RefSeq ORF: 2412
• Locus ID: 24627
• MW: 90.5 kDa
• Gene Summary: may contain eight isoforms that display differential expression and activities; isorofms Pde4D6 and Pde4D7 exhibit high affinity for cyclic AMP and are inhibited by rolipram [RGD, Feb 2006]