Pde4d (NM_001113329) Rat Tagged ORF Clone

CAT#: RR200500

Pde4d (Myc-DDK-tagged ORF) - Rat phosphodiesterase 4D, cAMP-specific (phosphodiesterase E3 dunce homolog, Drosophila) (Pde4d), transcript variant 2, (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Pde4d
• Synonyms: DPDE3; PDE3/IVd
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR200500 representing NM_001113329
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGAGGAACACTTGCGATGTACTTTCTCGGAGCAAAAGTGCCTCTGAGGAAACATTGCACTCCTGTA
  ATGATGAAGAGGACCCCTTCCGGGGAATGGAGCCCTATCTCGTACGGAGACTTTCATCCCGCAGCATCCA
  GCTTCCTCCTCTTGCCTTCAGACAGTTGGAGCAAACGGACTTGCGAAGCGAATCAGAGAACATTCCGCGG
  CCAACTAGCCTCCCCTTGAAAATCCTGCCGCTGATTGCTGTCACATCCGCAGACTCGACTGGTTTCGATG
  TGGACAATGGCACATCAGCGGGACGGAGTCCCTTGGATCCCATGACCAGCCCAGGGTCTGGGCTAATTCT
  CCAAGCAAACTTTGTGCACAGTCAACGCCGGGAGTCCTTCCTGTATCGATCAGACAGCGATTATGACCTC
  TCTCCAAAGTCTATGTCCAGGAACTCCTCGATTGCCAGTGATATACATGGAGATGACTTGATTGTGACTC
  CATTTGCTCAGGTCTTGGCCAGCCTTCGAACTGTAAGAAACAACTTTGCTGCATTAACTAATTTGCAAGA
  TCGCGCACCCAGCAAAAGATCACCCATGTGCAACCAACCATCCATCAACAAAGCCACCATCACAGAGGAG
  GCCTACCAGAAACTGGCCAGCGAGACCCTGGAGGAGCTGGACTGGTGTCTGGACCAGCTAGAGACCCTGC
  AGACCAGGCACTCCGTCAGTGAGATGGCCTCCAACAAGTTCAAGAGGATGCTTAATCGGGAGCTCACCCA
  CCTCTCTGAAATGAGTCGGTCTGGCAACCAGGTGTCGGAGTACATATCAAACACGTTCTTAGATAAGCAA
  CATGAAGTAGAAATTCCCTCTCCGACTCAGAAGGAAAAAGAGAAGAAGAAAAGGCCGATGTCACAGATCA
  GTGGGGTCAAGAAGTTGATGCACAGCTCCAGCCTGACCAATTCCTGCATTCCAAGATTTGGGGTTAAAAC
  AGAGCAGGAAGATGTCCTGGCCAAGGAACTAGAAGACGTGAACAAGTGGGGCCTCCACGTTTTCCGAATA
  GCGGAGCTGTCTGGCAACCGGCCTCTGACTGTTATCATGCACACCATTTTTCAGGAACGAGATTTGTTAA
  AAACGTTTAAAATCCCAGTGGACACTTTGATTACGTATCTTATGACTCTAGAAGACCATTACCATGCTGA
  CGTGGCCTATCACAACAACATCCATGCTGCAGATGTCGTCCAGTCAACTCATGTGCTGCTCTCTACACCC
  GCTTTGGAGGCTGTTTTCACTGACTTGGAGATTCTCGCGGCCATTTTTGCCAGTGCAATACATGATGTGG
  ATCATCCTGGTGTGTCAAATCAATTTCTGATCAATACAAACTCGGAACTTGCCTTGATGTACAACGACTC
  CTCCGTCTTAGAGAATCATCATTTGGCTGTGGGCTTTAAGTTGCTCCAGGAAGAAAACTGTGACATTTTC
  CAGAATCTGACCAAAAAGCAAAGACAATCTTTAAGGAAAATGGCCATTGACATTGTACTAGCGACAGACA
  TGTCAAAGCACATGAATCTGCTGGCTGATCTGAAAACAATGGTTGAAACGAAGAAGGTGACGAGCTCTGG
  CGTCCTCCTCCTTGATAACTATTCTGACAGGATCCAGGTCCTCCAGAATATGGTGCACTGTGCAGACCTG
  AGCAACCCCACAAAGCCACTCCAGCTCTACCGCCAGTGGACGGACCGGATAATGGAGGAGTTCTTCCGTC
  AGGGGGACCGGGAGCGTGAGCGTGGCATGGAGATAAGTCCCATGTGTGACAAGCACAACGCCTCTGTGGA
  AAAATCACAGGTGGGCTTCATAGACTACATTGTTCATCCACTCTGGGAGACATGGGCAGACCTCGTACAT
  CCCGACGCCCAGGACATTTTGGACACTTTGGAGGACAATCGTGAGTGGTACCAGAGCACAATCCCCCAGA
  GCCCCTCCCCTGCACCTGATGACCAAGAGGACGGCCGTCAGGGACAGACTGAAAAATTCCAGTTCGAACT
  AACCTTAGAGGAAGATGGCGAGTCAGACACTGAAAAGGACAGTGGAAGTCAAGTGGAGGAAGACACTAGC
  TGCAGTGACTCTAAGACTCTGTGCACCCAAGACTCAGAGTCCACTGAAATTCCCCTTGACGAGCAGGTTG
  AAGAGGAGGCTGTAGCAGAAGAGGAAAGCCAGCCCCAAACTGGCGTCGCAGACGATTGTTGTCCTGACAC
  G
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR200500 representing NM_001113329
  Red=Cloning site Green=Tags(s)
  
  MERNTCDVLSRSKSASEETLHSCNDEEDPFRGMEPYLVRRLSSRSIQLPPLAFRQLEQTDLRSESENIPR
  PTSLPLKILPLIAVTSADSTGFDVDNGTSAGRSPLDPMTSPGSGLILQANFVHSQRRESFLYRSDSDYDL
  SPKSMSRNSSIASDIHGDDLIVTPFAQVLASLRTVRNNFAALTNLQDRAPSKRSPMCNQPSINKATITEE
  AYQKLASETLEELDWCLDQLETLQTRHSVSEMASNKFKRMLNRELTHLSEMSRSGNQVSEYISNTFLDKQ
  HEVEIPSPTQKEKEKKKRPMSQISGVKKLMHSSSLTNSCIPRFGVKTEQEDVLAKELEDVNKWGLHVFRI
  AELSGNRPLTVIMHTIFQERDLLKTFKIPVDTLITYLMTLEDHYHADVAYHNNIHAADVVQSTHVLLSTP
  ALEAVFTDLEILAAIFASAIHDVDHPGVSNQFLINTNSELALMYNDSSVLENHHLAVGFKLLQEENCDIF
  QNLTKKQRQSLRKMAIDIVLATDMSKHMNLLADLKTMVETKKVTSSGVLLLDNYSDRIQVLQNMVHCADL
  SNPTKPLQLYRQWTDRIMEEFFRQGDRERERGMEISPMCDKHNASVEKSQVGFIDYIVHPLWETWADLVH
  PDAQDILDTLEDNREWYQSTIPQSPSPAPDDQEDGRQGQTEKFQFELTLEEDGESDTEKDSGSQVEEDTS
  CSDSKTLCTQDSESTEIPLDEQVEEEAVAEEESQPQTGVADDCCPDT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001113329
• ORF Size: 2241 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001113329.1, NP_001106800.1
• RefSeq Size: 2385
• RefSeq ORF: 2244
• Locus ID: 24627
• MW: 84.4 kDa
• Gene Summary: may contain eight isoforms that display differential expression and activities; isorofms Pde4D6 and Pde4D7 exhibit high affinity for cyclic AMP and are inhibited by rolipram [RGD, Feb 2006]