Gls (NM_012569) Rat Tagged ORF Clone

CAT#: RR200515

Gls (Myc-DDK-tagged ORF) - Rat glutaminase (Gls), nuclear gene encoding mitochondrial protein, transcript variant 1, (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Gls
• Synonyms: Glut; RATGLUT
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR200515 representing NM_012569
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGCGGCTGCGAGGCTCGGCGATGCTGCGGGAGCTGCTCTTGAGGCCGCCTGCCGCCGTCGGAGGCG
  TCTTGCGGCGCGCGCAGCCCCTCGGTACGCTGTGCCGGCGCCCCCGGGGCGGGAGCCGGCCGGCCGCTGG
  CCTGGTGGCCGCTGCGCGACTCCACCCGTGGTGGGGCGGGGGCGGCCGCGCGAAGGGCCCGGGCTCGGGC
  GGCCTGTCCAGCTCGCCCTCGGAGATCCTGCAGGAGCTGGGCAAGGGGGGCACGCCGCCACAGCAGCAGC
  AGCAGCAGCAGCAGCAGCCAGGGGCGTCGCCACCCGCAGCCCCGGGCCCCAAGGACAGCCCGGGGGAGAC
  GGACGCGTTCGGCAACAGCGAAGGCAAGGAGATGGTGGCTGCGGGCGACAATAAAGTAAAGCAGGGTCTG
  TTACCTAGCTTGGAAGATTTGCTGTTCTATACAATTGCAGAAGGACAAGAAAAGATACCTGTTCACAAGT
  TTATTACAGCACTCAAATCTACAGGATTGCGAACATCTGATCCCAGGTTGAAAGAGTGTATGGATATGTT
  AAGATTAACTCTTCAGACAACGTCAGATGGTGTCATGCTAGACAAAGATCTTTTTAAAAAGTGTGTTCAA
  AGCAACATCGTTTTGTTAACACAAGCATTTAGAAGAAAGTTTGTCATCCCTGACTTTATGTCTTTTACCT
  CACACATCGATGAGTTATATGAAAGTGCTAAAAAGCAGTCTGGAGGGAAGGTTGCTGATTATATTCCTCA
  ACTGGCCAAATTCAGTCCTGATTTGTGGGGTGTATCTGTTTGTACCGTAGATGGGCAAAGGCATTCTATT
  GGAGATACCAAAGTTCCCTTTTGTCTTCAGTCCTGTGTAAAGCCCCTGAAATATGCAATTGCTGTCAATG
  ACCTGGGAACTGAGTATGTGCATCGCTATGTTGGGAAGGAGCCAAGTGGATTAAGATTCAACAAGCTCTT
  TTTGAATGAAGATGATAAACCACATAATCCGATGGTAAATGCTGGAGCAATTGTTGTGACTTCACTAATA
  AAGCAAGGAGTAAATAATGCTGAGAAATTTGACTATGTGATGCAATTTTTGAATAAGATGGCTGGTAATG
  AATATGTCGGATTCAGTAATGCAACGTTTCAATCTGAACGAGAAAGTGGAGACCGAAATTTTGCAATAGG
  ATATTACTTAAAAGAAAAGAAGTGTTTTCCAGAAGGCACAGACATGGTTGGGATACTAGATTTTTATTTC
  CAGCTCTGTTCCATTGAAGTGACGTGTGAGTCAGCAAGTGTGATGGCTGCAACCTTGGCTAATGGCGGTT
  TCTGCCCAATTACTGGTGAAAGAGTGCTCAGTCCTGAGGCCGTTCGGAATACCCTGAGCTTAATGCACTC
  CTGTGGCATGTATGACTTCTCAGGGCAGTTTGCATTTCATGTTGGTCTTCCTGCAAAATCTGGAGTTGCT
  GGGGGTATTCTTTTAGTTGTCCCCAACGTCATGGGCATGATGTGTTGGTCTCCTCCTCTTGACAAGATGG
  GCAACAGTGTTAAAGGAATTCACTTTTGTCACGATCTTGTTTCTCTGTGTAACTTCCATAACTATGATAA
  TTTGAGACACTTTGCAAAAAAACTTGATCCTCGGAGAGAAGGAGGTGATCAAAGGGTAAAGTCGGTGATA
  AACCTCCTGTTTGCTGCGTACACTGGAGATGTGTCTGCCCTCCGAAGGTTTGCTCTGTCAGCCATGGACA
  TGGAACAGAGAGATTACGACTCCAGAACAGCCCTGCATGTTGCTGCTGCAGAAGGTCATGTTGAAGTTGT
  GAAGTTTTTGCTGGAAGCTTGCAAAGTTAACCCCTTCCCCAAGGACAGGTGGAATAACACCCCCATGGAT
  GAAGCACTGCACTTTGGACACCATGATGTTTTTAAAATCCTCCAGGAATACCAAGTTCAGTATACACCTC
  AAGGGGATTCTGACGATGGAAAAGAAAACCAGACTGTCCACAAGAATCTTGACGGGTTGTTA
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR200515 representing NM_012569
  Red=Cloning site Green=Tags(s)
  
  MMRLRGSAMLRELLLRPPAAVGGVLRRAQPLGTLCRRPRGGSRPAAGLVAAARLHPWWGGGGRAKGPGSG
  GLSSSPSEILQELGKGGTPPQQQQQQQQQPGASPPAAPGPKDSPGETDAFGNSEGKEMVAAGDNKVKQGL
  LPSLEDLLFYTIAEGQEKIPVHKFITALKSTGLRTSDPRLKECMDMLRLTLQTTSDGVMLDKDLFKKCVQ
  SNIVLLTQAFRRKFVIPDFMSFTSHIDELYESAKKQSGGKVADYIPQLAKFSPDLWGVSVCTVDGQRHSI
  GDTKVPFCLQSCVKPLKYAIAVNDLGTEYVHRYVGKEPSGLRFNKLFLNEDDKPHNPMVNAGAIVVTSLI
  KQGVNNAEKFDYVMQFLNKMAGNEYVGFSNATFQSERESGDRNFAIGYYLKEKKCFPEGTDMVGILDFYF
  QLCSIEVTCESASVMAATLANGGFCPITGERVLSPEAVRNTLSLMHSCGMYDFSGQFAFHVGLPAKSGVA
  GGILLVVPNVMGMMCWSPPLDKMGNSVKGIHFCHDLVSLCNFHNYDNLRHFAKKLDPRREGGDQRVKSVI
  NLLFAAYTGDVSALRRFALSAMDMEQRDYDSRTALHVAAAEGHVEVVKFLLEACKVNPFPKDRWNNTPMD
  EALHFGHHDVFKILQEYQVQYTPQGDSDDGKENQTVHKNLDGLL
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_012569
• ORF Size: 2022 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_012569.1, NM_012569.2, NP_036701.2
• RefSeq Size: 4530
• RefSeq ORF: 2025
• Locus ID: 24398
• MW: 74 kDa
• Gene Summary: catalyzes the conversion of L-glutamine and H2O to L-glutamate and NH3 in glutamine catabolism [RGD, Feb 2006]