Eno1 (NM_001109908) Rat Tagged ORF Clone

CAT#: RR200517

Eno1 (Myc-DDK-tagged ORF) - Rat enolase 1, (alpha) (Eno1), transcript variant 2, (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Eno1
• Synonyms: Nne
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR200517 representing NM_001109908
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCATTCTCAAGATCCATGCCAGAGAGATCTTTGACTCCCGCGGGAATCCCACCGTTGAGGTGGATC
  TCTACACCGCAAAAGGTCTCTTCCGTGCTGCGGTGCCCAGCGGTGCGTCCACTGGCATCTACGAGGCCCT
  AGAACTCCGAGACAATGATAAGACCCGCTTCATGGGGAAGGGTGTCTCAAAGGCTGTTGAGCACATCAAT
  AAAACTATTGCACCTGCTCTGGTTAGCAAGAAACTGAATGTTGTGGAGCAGGAGAAGATTGACCAGCTGA
  TGATCGAGATGGACGGCACAGAGAATAAATCTAAGTTTGGTGCAAATGCCATCCTGGGAGTGTCCCTGGC
  TGTCTGCAAGGCTGGTGCCGTGGAGAAGGGGGTGCCCCTTTACCGTCACATTGCCGACTTGGCCGGCAAC
  CCTGAAGTCATCCTGCCGGTCCCAGCTTTCAATGTGATCAACGGCGGTTCTCATGCTGGCAACAAGTTGG
  CCATGCAAGAGTTCATGATCCTGCCTGTGGGGGCATCCTCTTTCCGGGAAGCCATGCGCATTGGAGCAGA
  GGTTTACCACAACCTGAAGAACGTCATCAAGGAGAAGTACGGGAAAGACGCCACCAATGTGGGTGATGAG
  GGTGGATTCGCACCTAACATCCTGGAGAACAAAGAAGCACTGGAGCTGCTCAAGTCTGCCATTGCAAAGG
  CCGGCTACACTGACCAGGTTGTCATCGGCATGGATGTGGCTGCCTCCGAGTTCTACAGGGCTGGCAAGTA
  TGACCTGGACTTCAAGTCTCCAGATGATGCCAGCCGGTACATCACACCCGACCAGCTGGCCGACCTGTAC
  AAGTCCTTCATCAAGGACTACCCAGTGGTGTCCATTGAAGATCCCTTTGACCAGGACGACTGGGATGCTT
  GGCAGAAGTTCACAGCTACTGCAGGCATCCAGGTGGTGGGGGATGACCTCACAGTGACCAACCCTAAGCG
  GATCGCCAAGGCTGCAGGCGAAAAGTCCTGCAACTGCCTCCTGCTCAAAGTGAACCAGATTGGCTCTGTG
  ACCGAGTCTCTGCAGGCGTGTAAGCTGGCCCAGTCCAATGGCTGGGGTGTCATGGTGTCCCATCGATCTG
  GGGAGACTGAGGACACTTTCATTGCCGACCTGGTGGTGGGGCTCTGCACTGGGCAGATCAAGACTGGTGC
  CCCCTGCCGATCTGAGCGCCTGGCCAAGTACAATCAGATCCTTAGAATCGAGGAGGAGCTGGGCAGCAAA
  GCCAAGTTTGCCGGCAGGTCCTTCAGGAACCCCCTGGCCAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR200517 representing NM_001109908
  Red=Cloning site Green=Tags(s)
  
  MSILKIHAREIFDSRGNPTVEVDLYTAKGLFRAAVPSGASTGIYEALELRDNDKTRFMGKGVSKAVEHIN
  KTIAPALVSKKLNVVEQEKIDQLMIEMDGTENKSKFGANAILGVSLAVCKAGAVEKGVPLYRHIADLAGN
  PEVILPVPAFNVINGGSHAGNKLAMQEFMILPVGASSFREAMRIGAEVYHNLKNVIKEKYGKDATNVGDE
  GGFAPNILENKEALELLKSAIAKAGYTDQVVIGMDVAASEFYRAGKYDLDFKSPDDASRYITPDQLADLY
  KSFIKDYPVVSIEDPFDQDDWDAWQKFTATAGIQVVGDDLTVTNPKRIAKAAGEKSCNCLLLKVNQIGSV
  TESLQACKLAQSNGWGVMVSHRSGETEDTFIADLVVGLCTGQIKTGAPCRSERLAKYNQILRIEEELGSK
  AKFAGRSFRNPLAK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001109908
• ORF Size: 1302 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001109908.1, NP_001103378.1
• RefSeq Size: 1803
• RefSeq ORF: 1305
• Locus ID: 24333
• MW: 47.1 kDa
• Gene Summary: Multifunctional enzyme that, as well as its role in glycolysis, plays a part in various processes such as growth control, hypoxia tolerance and allergic responses. May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. Stimulates immunoglobulin production. [UniProtKB/Swiss-Prot Function]