Add2 (NM_012491) Rat Tagged ORF Clone

CAT#: RR200529

Add2 (Myc-DDK-tagged ORF) - Rat adducin 2 (beta) (Add2), transcript variant 2, (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Add2
• Synonyms: beta-ADD
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR200529 representing NM_012491
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTGAGGACACGGTCCCCGAGGCAGCCTCACCGCCACCCTCTCAGGGGCAGCACTACTTTGACCGGT
  TCTCTGAGGATGACCCTGAATACTTGCGACTTCGCAACCGTGCAGCTGACCTGCGACAAGACTTCAACCT
  GATGGAGCAGAAGAAACGGGTCACCATGATCCTGCAGAGCCCTTCTTTCAGGGAGGAGCTGGAAGGCCTC
  ATCCAGGAACAGATGAAGAAGGGTAACAACTCCTCCAACATCTGGGCCCTCCGACAGATCGCGGACTTCA
  TGGCCAGCACCTCCCACGCAGTCTTCCCAGCTTCCTCCATGAACTTCTCCATGATGACACCCATCAATGA
  CCTCCACACTGCCGACTCCCTGAACCTGGCCAAGGGGGAGAGGCTTATGCGGTGCAAGATCAGCAGTGTC
  TACCGTCTTCTGGACCTCTATGGCTGGGCGCAGCTCAGCGACACCTATGTCACGCTGAGAGTGAGCAAGG
  AGCAGGACCACTTCCTGATCAGCCCCAAAGGGGTTTCCTGCAGTGAGGTCACGGCATCCAGCCTGATTAA
  GGTGAACATTCTAGGAGAGGTCGTGGAGAAGGGCAGCAGTTGCTTCCCAGTGGACACCACCGGCTTCAGT
  CTGCACTCAGCCATCTATGCGGCCAGGCCGGACGTGCGGTGTGCCATCCACCTGCACACGCCTGCCACAG
  CAGCGGTGTCAGCTATGAAGTGTGGCCTCCTGCCTGTCTCCCATAATGCCCTGCTGGTGGGGGACATGGC
  CTACTATGACTTCAATGGGGAAATGGAGCAGGAAGCTGATCGAATCAACTTGCAGAAGTGCCTTGGACCC
  ACCTGCAAGATTCTGGTGCTAAGAAACCATGGTATGGTCGCCCTGGGTGACACCGTGGAGGAAGCTTTCT
  ACAAGGTCTTCCACCTGCAGGCTGCGTGTGAGGTACAGGTGTCGGCTCTGTCCAGCGCTGGGGGGACCGA
  GAACCTCATCCTCCTGGAGCAAGAGAAACACCGTCCGCATGAGGTGGGCTCTGTGCAGTGGGCCGGCAGC
  ACCTTTGGGCCCATGCAGAAGAGCCGGCTGGGAGAGCATGAATTTGAAGCCCTCATGAGGATGCTCGACA
  ACTTAGGCTACAGAACAGGCTATACGTACCGCCACCCCTTCGTCCAAGAGAAAACCAAACACAAAAGTGA
  AGTGGAGATCCCAGCCACGGTCACTGCCTTTGTGTTCGAAGAGGATGGTGTCCCAGTCCCTGCTCTGCGC
  CAGCACGCCCAAAAGCAGCAGAAGGAAAAGACCCGCTGGCTTAACACTCCCAACACCTACCTGAGGGTGA
  ATGTGGCCGACGAGGTGCAGAGGAACATGGGCAGTCCCCGACCGAAGACCACGTGGATGAAGGCTGATGA
  AGTGGAAAAGTCCAGCAGCGGCATGCCCATACGGATTGAAAACCCAAACCAATTTGTGCCTCTCTACACT
  GACCCCCAAGAAGTGCTGGACATGAGGAACAAGATTCGAGAACAAAACCGACAAGATATAAAGTCAGCCG
  GGCCTCAGTCTCAGCTCTTGGCCAGCGTCATCGCCGAGAAGAGCCGGAGTCCGGTACAGCAGAGACTGCC
  CCCAACTGAAGGGGAAGCTTATCAGACTCCTGGGGCTGGGCAGGGGACCCCTGAGTCCTCAGGCCCACTC
  ACCCCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR200529 representing NM_012491
  Red=Cloning site Green=Tags(s)
  
  MSEDTVPEAASPPPSQGQHYFDRFSEDDPEYLRLRNRAADLRQDFNLMEQKKRVTMILQSPSFREELEGL
  IQEQMKKGNNSSNIWALRQIADFMASTSHAVFPASSMNFSMMTPINDLHTADSLNLAKGERLMRCKISSV
  YRLLDLYGWAQLSDTYVTLRVSKEQDHFLISPKGVSCSEVTASSLIKVNILGEVVEKGSSCFPVDTTGFS
  LHSAIYAARPDVRCAIHLHTPATAAVSAMKCGLLPVSHNALLVGDMAYYDFNGEMEQEADRINLQKCLGP
  TCKILVLRNHGMVALGDTVEEAFYKVFHLQAACEVQVSALSSAGGTENLILLEQEKHRPHEVGSVQWAGS
  TFGPMQKSRLGEHEFEALMRMLDNLGYRTGYTYRHPFVQEKTKHKSEVEIPATVTAFVFEEDGVPVPALR
  QHAQKQQKEKTRWLNTPNTYLRVNVADEVQRNMGSPRPKTTWMKADEVEKSSSGMPIRIENPNQFVPLYT
  DPQEVLDMRNKIREQNRQDIKSAGPQSQLLASVIAEKSRSPVQQRLPPTEGEAYQTPGAGQGTPESSGPL
  TP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_012491
• ORF Size: 1686 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_012491.1, NM_012491.2, NP_036623.1
• RefSeq Size: 3268
• RefSeq ORF: 1689
• Locus ID: 24171
• MW: 62.8 kDa
• Gene Summary: plays a role in membrane ion transport; variation is associated with blood pressure variation in the Milan hypertensive strain [RGD, Feb 2006]