Acsm5 (NM_001014162) Rat Tagged ORF Clone

CAT#: RR200595

Acsm5 (Myc-DDK-tagged ORF) - Rat acyl-CoA synthetase medium-chain family member 5 (Acsm5), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Acsm5
• Synonyms: Aa2-174; Cc1-38; RGD1309578
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR200595 representing NM_001014162
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGTTATGGCTGAGAGGTCTGGTCTACCAGGCTCGGAGGAGTTCCTGGGGAGTCTTCAGGATTCACA
  CACAGCCACCACCTCCCCCTATCCCTGAGGTTGTAGCTACCTGGGAAGCCATCAGCCTAGGGAAGCGGCC
  AGTGCCGGAGTACTTCAACTTTGCCCACGATGTGCTAGATGTGTGGAGTCAGCTGGAAAAGACAGGGCAC
  CGCCCCCCAAATCCTGCATTCTGGTGGGTGAATGGCTCAGGAACAGAAGTCAAGTGGACATTTGAGGAAC
  TGGGAAAGCAGTCCAGGAAGGCAGCCAACATCTTAGAGGGAGCATGTGGCCTGAAACCTGGAGACAGATT
  AATGCTGGTGCTTCCGAGACTCCCAGAATGGTGGCTGACCATCGTGGCTTGTATGCGAACAGGTGTGGTC
  ATGATCCCAGGCATTTCCCAGCTGACACAGAAGGATCTCAAGTACCGGCTACAGGCTGCCAGGGTCAAAT
  CCATCATCACCAGTGATGCCCTAGCTCCTCACGTGGATGCCATCAGTGCTGACTGCCCCTCCCTTCAAAG
  CAGGCTTCTGGTGTCTGATACCAGTCGTCCAGGCTGGATCAATTTCCGGGAACTCCTGAGAGTGGCATCT
  CCAGAACACAACTGCCTGAGAACCAGAAGTGGAGATTCAATGGCCATCTACTTCACCAGTGGAACCACGG
  GGACCCCCAAGATGGTGGAACATTCCCAGTGCAGCTATGGACTGGGTTTTGTGGCCAGTGGAAGGAGGTT
  GATGGCCTTGACTGAATCCGACATATTCTGGAACACAACTGACACTGGTTGGGTGAAAGCAGCCTGGACT
  CTCTTCTCTGCCTGGGCCAATGGCGCTTGCGTTTTTGTGCATGAGCTGCCCCAAGTTGATGCCCAAACTA
  TTCTAAATACTCTCTGCAGGTTCCCAATAACCACCATTTGCTGTGTCCCAACCCTCTTCCGGCTGCTTGT
  ACAAGAGGATCTGACAAGGTACAAGTTCCAGTGCCTCAGACACTGTTTGGCCGGTGGAGAAGCCCTCAAC
  TCTGACGTGAGGGACAAGTGGAAGAACCAGACAGGCCTGGAGATCCATGAAGGCTACGGACAATCAGAAA
  CAGTCCTCATCTGTGGCAATTTTAGAGGCTCAACGATCAAGTCTGGATCTATGGGGAAAGCAAGCCCACC
  TTACGATGTGCAGATTGTAGACGAGGAGGGTAATGTCCTGCCTCCAGGAAAAGAGGGAAATATTGCCATC
  CGCATCAAACCTACTAGACCCTTCTGTCTCTTCAACTGCTATCTGGACAATCCTGAGAAGACAGCAGCAT
  CAGAGCAAGGAGACTTTTACATCACTGGGGACCGAGCACATATGGACGAGGACGGCTACTTTTGGTTCGT
  GGGGAGAAATGATGATGTAATCAACTCTTCAAGCTACCGGATTGGGCCTGTGGAAGTAGAGAGTGCACTG
  GCTGAGCATCCGGCTGTCCTGGAGTCTGCTGTGGTCAGCAGCCCAGACCCCATCCGGGGAGAGGTGGTAA
  AGGCGTTTATAGTCCTTTCTCCAGCCTATGTGTCTCATGACCCAGAAGCCCTGACCAGGGAACTCCAGGA
  GCATGTGAAGACAGTGACTGCTCCATACAAGTACCCCAGGAAGGTGGCTTTTATTTCGGAACTGCCAAAG
  ACGGTTTCTGGAAAGATCCTAAGGAGTAAATTGAGAAACCAAGAGTGGGGGAGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR200595 representing NM_001014162
  Red=Cloning site Green=Tags(s)
  
  MRLWLRGLVYQARRSSWGVFRIHTQPPPPPIPEVVATWEAISLGKRPVPEYFNFAHDVLDVWSQLEKTGH
  RPPNPAFWWVNGSGTEVKWTFEELGKQSRKAANILEGACGLKPGDRLMLVLPRLPEWWLTIVACMRTGVV
  MIPGISQLTQKDLKYRLQAARVKSIITSDALAPHVDAISADCPSLQSRLLVSDTSRPGWINFRELLRVAS
  PEHNCLRTRSGDSMAIYFTSGTTGTPKMVEHSQCSYGLGFVASGRRLMALTESDIFWNTTDTGWVKAAWT
  LFSAWANGACVFVHELPQVDAQTILNTLCRFPITTICCVPTLFRLLVQEDLTRYKFQCLRHCLAGGEALN
  SDVRDKWKNQTGLEIHEGYGQSETVLICGNFRGSTIKSGSMGKASPPYDVQIVDEEGNVLPPGKEGNIAI
  RIKPTRPFCLFNCYLDNPEKTAASEQGDFYITGDRAHMDEDGYFWFVGRNDDVINSSSYRIGPVEVESAL
  AEHPAVLESAVVSSPDPIRGEVVKAFIVLSPAYVSHDPEALTRELQEHVKTVTAPYKYPRKVAFISELPK
  TVSGKILRSKLRNQEWGR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001014162
• ORF Size: 1734 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001014162.1, NM_001014162.3, NM_001014162.4, NP_001014184.3
• RefSeq Size: 2457 bp
• RefSeq ORF: 1737 bp
• Locus ID: 361637
• Cytogenetics: 1q35
• MW: 64.6 kDa