Ubox5 (NM_001033997) Rat Tagged ORF Clone

CAT#: RR200676

Ubox5 (Myc-DDK-tagged ORF) - Rat U-box domain containing 5 (Ubox5), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ubox5
• Synonyms: RGD1305440
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR200676 representing NM_001033997
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTAATAAATCTTTGCCTCCCTCAGTTCAGACCAAGAATTCACTGCAACAAGGTGTCAGCTGATGGTT
  ATGAAGTAGAAAATCTCATCTCTGAAGACCTCATAAAGAGAAGCCATGGCTTTAGGACAGAATATTTCAT
  TAGACCTCCAATCTACGTGACAGTTTCCTTTCCCTTTAATGTGGAGATCTGTAGGGTTAACATAGATCTC
  ACAGCTGGGGGATGTCAGAATGTCACTGGCCTGGAATTATATACTTCTGCCTTATCTAGCAGAGCCCCTC
  AGGATGCACAGGACTGCTGGACTACAGGTCTGAGCGAGACATCTGTCCCAGACAAGGAGGCCTTCACCTT
  GGTAGGCAAAGTCTTACTGAAAAACCAGAACCATGTAGTGTTCAGTCACAGGGGCTTCAAGGCCAGGCCA
  CCTTTCAGTCCAATGGAAGTCACACTCCTCTCTCCTGCTGTTGTAGCGCAGGATCTCTGGAATAAAGGGG
  CTTTTTCCCTCAGCCATGTGGCCCACCTCAAGATCGGCATCACCCATGTGACAGGCAGTGGCATCTCTTG
  CATCAAGCGTTTAGAGGTGTGGGGTCAGCCAGCCAGGACCTGCTCTCAGGAGGTGATAAATAGCATCTTG
  TCGACAGCCTCAGAAAGCCCTTCTCAGGACTTGGGTCTGCACACTCCAGCCTTGCCCATGGAGAGTGACT
  ATGACCCTGGGGGTCAATCTGAGGGCCAGCAGTCTCCCTGTACCTTACAGGACATGTCTGAGGTCGTTCA
  AGACGTGCCAGAGGAGTTCCTGGATCCTATCACCCTGGAGATCATGCCGTGCCCCATGCTTCTGCCCTCG
  GGCAAGGTCATTGACCAGAGCACTCTGGAGAAGTGTAACCTCAGTGAAGCTGCTTGGGGCCGAGTGCCCA
  GTGACCCTTTCACAGGGTTAGCCTTTACTCCACAGTCCCAGCCCCTACCTCATCCTTCCTTAAAGGCCCG
  GATCGACCGTTTCCTACTCCAACACTCTATTTCAGGCTGCCACCTGCTTGGGAGAGCACAGACTGCATCA
  GCAATGACCCCTTCTATCATCACTCTGCCCTCAAGGAAAAGGAAGACAGGGCAGGCTGAGCACAGCTCAG
  ACTATAGTCTTGGCATGAAGGCTTCTTCCTCTGCCACAAGCCCTTTACTCTCACCTACTACCTCAGAGCC
  GACTGCCAAGAAGATGAAAGGTGCCAGCGAACTGGGCCTGACAGACATGGACTGTTCAGCAGGTCCCGTG
  TCCCATGAGCAGAAGCTGTCGCAAAGTCTGGAAATTGCCCTGACTTCCACCCTTGGCTCCATGCCTTCCT
  TCACTGCCCGGCTCACCAAGGGGCAGCTCCAGCTGGGCACAAGAGGGAGCAGTGCTTGCAGGAGGCCAGC
  CACCAGCTCCGAGCTTCCAAGGAGTGCTGGCCCAGAGTGTGCCTCCTGCAGACGAGTGTTTTCTTCCTAC
  ACCACCAATGAGCCTGTGTACCAGCTGCCCTGTGGCCACCTTCTGTGCCGACCCTGCCTGAGTGAGAAGC
  AACGTTCCCAGCCCATGATGTGCACAGCCTGCCAGCAGCCGGTCACCAGCCAGGATGTGCTGCGGGTCCA
  TTTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR200676 representing NM_001033997
  Red=Cloning site Green=Tags(s)
  
  MVINLCLPQFRPRIHCNKVSADGYEVENLISEDLIKRSHGFRTEYFIRPPIYVTVSFPFNVEICRVNIDL
  TAGGCQNVTGLELYTSALSSRAPQDAQDCWTTGLSETSVPDKEAFTLVGKVLLKNQNHVVFSHRGFKARP
  PFSPMEVTLLSPAVVAQDLWNKGAFSLSHVAHLKIGITHVTGSGISCIKRLEVWGQPARTCSQEVINSIL
  STASESPSQDLGLHTPALPMESDYDPGGQSEGQQSPCTLQDMSEVVQDVPEEFLDPITLEIMPCPMLLPS
  GKVIDQSTLEKCNLSEAAWGRVPSDPFTGLAFTPQSQPLPHPSLKARIDRFLLQHSISGCHLLGRAQTAS
  AMTPSIITLPSRKRKTGQAEHSSDYSLGMKASSSATSPLLSPTTSEPTAKKMKGASELGLTDMDCSAGPV
  SHEQKLSQSLEIALTSTLGSMPSFTARLTKGQLQLGTRGSSACRRPATSSELPRSAGPECASCRRVFSSY
  TTNEPVYQLPCGHLLCRPCLSEKQRSQPMMCTACQQPVTSQDVLRVHF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001033997
• ORF Size: 1614 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001033997.1, NP_001029169.1
• RefSeq Size: 3686 bp
• RefSeq ORF: 1617 bp
• Locus ID: 296161
• Cytogenetics: 3q36
• MW: 58.4 kDa