Lsg1 (NM_001013421) Rat Tagged ORF Clone

CAT#: RR200747

Lsg1 (Myc-DDK-tagged ORF) - Rat large subunit GTPase 1 homolog (S. cerevisiae) (Lsg1), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Lsg1
• Synonyms: RGD1309089
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR200747 representing NM_001013421
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCCGGAGAAGAGCGCCGGGTGGTGGGTCACTGGGTCGGGTACTTATCCGGCATCAGACTCAACGAA
  GCCGCAGTCACCGTCATACTGACTCCTGGCTGCATACGAGTGAACTCAATGATGGTTATGATTGGGGACG
  TCTGAATCTTCAGTCAGTGACGGAACAGAGCTCCCTCGAGGACTTTCTTGCAACTGCAGAACTTGCAGGG
  ACGGAGTTCGTGGCTGAGAAGCTTAATATTAAGTTTGTGCCCCCTGAGGCTAGAACTGGACTGCTGTCTT
  TCGAGGAGAGCCAGAGAATTAAGAGACTCCATGAAGAAAACAGACAGTTCCTGTGTATACCAAGGAGACC
  AAATTGGGACAGAAAAACTAGCCCTGAAGAACTGAAACAAGCAGAGAAAGATAACTTTCTAAAATGGAGG
  CGCCAGCTTGTCCGGCTAGAAGAAGAACAGAAGTTGATATTGACTCCCTTTGAACGGAATTTGGACTTCT
  GGCGCCAGCTCTGGAGGGTCATTGAGAGAAGTGATATTGTGGTCCAGATAGTAGATGCTCGGAACCCGCT
  CCTGTTCAGATGTGAGGATTTGGAATGTTACGTGAAGGAGATAGATGCTGCCAAGGAGAATGTGATTCTG
  ATAAACAAGGCAGACTTGTTGACTGCTGAGCAGAGGGTTGCCTGGGCTGTGCACTTTGAGAAAGAAGGTG
  TGAAGGTTATCTTCTGGTCAGCTTTGGCAGAAACCGTTCACCTAAATGGTGACTCTAAGGATGAAGTAAA
  CAGTGTTGCTGGAGAAGCCAACAGCTCTGAGTCTGAAGACTCCAGTTTGGACGGGAATGAAATTCCACAC
  AGAGACTTGTTCTTGCTTAGTGAGGAGTCCGAAAGTGACGATGACGACAGTGAGTATGAGGACTGTCAGG
  AGGACGAGGAGGAAGATTGGCAGACGTGTTCTGAAGAAGATAGCAATCCTGAGGAGGGGCAGGAGGAAGG
  GGGCTGTGATCGGGACCAGAAGGAACACGGTCCTGAGGATTCTGAGGCTCAGAGCAGAGCATCACCAGAA
  AATTCACAGATGAGCAACAAGAGCCATCTGGTCTCGAAGCAGGAGTTACTAGAGCTCTTCAAGAAACTCC
  ACACTGGGAAGAAGGTGAAAGACGGACAGCTGACTGTAGGGCTGGTGGGCTACCCTAATGTTGGTAAGAG
  CTCAACCATCAACACCATCATGGGCAACAAGAAAGTATCGGTGTCTGCCACGCCAGGCCACACCAAGCAT
  TTCCAGACCCTGTACGTGGAGCCAGGCCTCTGTCTGTGTGACTGCCCCGGCCTGGTGATGCCATCTTTTG
  TGTCTACCAAAGCAGAGATGATTTGCAGTGGGATCCTCCCTATCGACCAGATGAGAGATCACGTTCCGCC
  TGTATCACTAGTTTGCCAGAATATCCCGAGGCGAGTTTTAGAAGCTACCTATGGCATTAACATTATAAAG
  CCTGGAGAAGATGAAGACCCCTACCGACCTCCGACCTCAGAAGAGCTGTTGACAGCTTATGGATGCATGC
  GGGGATTCATGACAGCACACGGACAGCCAGACCAGCCTCGGTCTGCACGATACATCCTGAAGGACTACGT
  CAGAGGTAAACTTCTGTATTGTCACCCTCCTCCTGGAAAAGACCCTGTGGCATTCCAGCATCAACACAGG
  CAACTCCTAGAGAACAAAATAAAAGGTGAAGAACTGAGGCTTCAGCCAGGCAAAACCCAAAAAGCCAAAC
  AGGTTGAAAATGTAGTCGACAAGACTTTTTTCCATCAGGAGAATGTCAGAGCATTGACCAAAGGGGTCCA
  GGCTGTCATGGGTTACAAGCCCGGGAGTGGCCTGGTGACTGCAGCTGCTGCGAGTGCTGAGAACGTACCT
  GGGAAGCCCTGGAAAAAACACGGTAACAGAAACAAAAAAGAGAAAAGCCGCAGGCTCTACAGGCACCTGG
  ATGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR200747 representing NM_001013421
  Red=Cloning site Green=Tags(s)
  
  MGRRRAPGGGSLGRVLIRHQTQRSRSHRHTDSWLHTSELNDGYDWGRLNLQSVTEQSSLEDFLATAELAG
  TEFVAEKLNIKFVPPEARTGLLSFEESQRIKRLHEENRQFLCIPRRPNWDRKTSPEELKQAEKDNFLKWR
  RQLVRLEEEQKLILTPFERNLDFWRQLWRVIERSDIVVQIVDARNPLLFRCEDLECYVKEIDAAKENVIL
  INKADLLTAEQRVAWAVHFEKEGVKVIFWSALAETVHLNGDSKDEVNSVAGEANSSESEDSSLDGNEIPH
  RDLFLLSEESESDDDDSEYEDCQEDEEEDWQTCSEEDSNPEEGQEEGGCDRDQKEHGPEDSEAQSRASPE
  NSQMSNKSHLVSKQELLELFKKLHTGKKVKDGQLTVGLVGYPNVGKSSTINTIMGNKKVSVSATPGHTKH
  FQTLYVEPGLCLCDCPGLVMPSFVSTKAEMICSGILPIDQMRDHVPPVSLVCQNIPRRVLEATYGINIIK
  PGEDEDPYRPPTSEELLTAYGCMRGFMTAHGQPDQPRSARYILKDYVRGKLLYCHPPPGKDPVAFQHQHR
  QLLENKIKGEELRLQPGKTQKAKQVENVVDKTFFHQENVRALTKGVQAVMGYKPGSGLVTAAAASAENVP
  GKPWKKHGNRNKKEKSRRLYRHLDV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001013421
• ORF Size: 1965 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001013421.1, NP_001013439.1
• RefSeq Size: 2283 bp
• RefSeq ORF: 1968 bp
• Locus ID: 288029
• Cytogenetics: 11q22
• MW: 74.4 kDa