Stap2 (NM_001025026) Rat Tagged ORF Clone

CAT#: RR200861

Stap2 (Myc-DDK-tagged ORF) - Rat signal transducing adaptor family member 2 (Stap2), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Stap2
• Synonyms: MGC108972
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR200861 representing NM_001025026
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCTCAGCCCTGTGTCCACCCCGGGGCCCCAAGCTCAAAGGTGCTCCACCTTCCCACTACTACGAGA
  ACTTCCTAGAGAAAAAGGGACCCTGTGACCAGGATTACAGGAAGTTCTGGGCGGGCCTCCAGGGTTTAGC
  CATCTGTTTCTACAACAGCAATCGGGACTTGCAGCCCCTGGAAAAGCTGGACCTGAGGCTGTTTTCAAAG
  CTCAAAGATGAGGCTCTGTTGGGAAGCTCACATGACACTACCTATCACTTCAGCCTGGTTCTCCGGGACC
  AGGAGGTTAAATTCAAGGTGGAGAGCCTGGAGTCTTGTGAGATGTGGAAAGGATTTATCTTGACTGTGGT
  GGAGCTCCGTGTCCCATCTAACCTGACCCTGCTGCCCGGACACATGTACATGATGGCTGAAGTCCTGGCC
  AAAGAGGAAGTACGAAGGGCAGCCGAGGTGCCCTGGTGCTTTCTGCAGGTGAGCAGGCTAGAGGCGCAGC
  TGCTCCTGGAGCGCTACCCGGAGTGCGGGAACCTGCTTCTGCGACCTGGAGGGGATGGCAAGGACAGTGT
  GTCTGTCACTACCCGGCAGATACTCAATGGGTCGCCGGTGATCAAACACTACAAGGTGAAGCGAGAAGGT
  TCTAAGTATGTCATCGACGTAGAAGACCCGTTTTCCTGCCCCTCCCTGGAAGCTGTGGTCAACTATTTTG
  TGACGCACACCAAGAGGGCGCTGGTCCCCTTCCTGTTAGATGAGGACTATGAGAAGGTTCTAGGCTTCGT
  GGATTCGGATCGGGAGAATGGCGAGAATGCATGGACTGTGCCCTCCTCCCGAGGCTCAGGCCCTGCCCTC
  CCTGCTAATGTCCCAAAGCCACTCCCACCTGTACCTGTGTCTGTGTCCAGCCAGGAAGACAAGCTGCCTC
  AACTACCTCCCCTGCCTCAGTTACCAGACCAGGATGAGAACTATGTGACTCCCATTGAAGACTCCCTAGC
  AACTGAATACATGAATCGGGATGATCTCAAGGCCATTACCAACATCTGGACCAGGAAACTAGGTGGAAGC
  TCACCCCAGGCTTCCTCCCTTGTGACAAGACTTGGGGATATCACCGCTGAGCTGGAAGAGAAACTACAGA
  AGAGGAGGGCACTGGAACAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR200861 representing NM_001025026
  Red=Cloning site Green=Tags(s)
  
  MASALCPPRGPKLKGAPPSHYYENFLEKKGPCDQDYRKFWAGLQGLAICFYNSNRDLQPLEKLDLRLFSK
  LKDEALLGSSHDTTYHFSLVLRDQEVKFKVESLESCEMWKGFILTVVELRVPSNLTLLPGHMYMMAEVLA
  KEEVRRAAEVPWCFLQVSRLEAQLLLERYPECGNLLLRPGGDGKDSVSVTTRQILNGSPVIKHYKVKREG
  SKYVIDVEDPFSCPSLEAVVNYFVTHTKRALVPFLLDEDYEKVLGFVDSDRENGENAWTVPSSRGSGPAL
  PANVPKPLPPVPVSVSSQEDKLPQLPPLPQLPDQDENYVTPIEDSLATEYMNRDDLKAITNIWTRKLGGS
  SPQASSLVTRLGDITAELEEKLQKRRALEH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001025026
• ORF Size: 1140 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001025026.1, NP_001020197.1
• RefSeq Size: 1439 bp
• RefSeq ORF: 1143 bp
• Locus ID: 363334
• Cytogenetics: 9q12
• MW: 42.8 kDa