Adgre1 (NM_001007557) Rat Tagged ORF Clone

CAT#: RR200880

Emr1 (Myc-DDK-tagged ORF) - Rat EGF-like module containing, mucin-like, hormone receptor-like 1 (Emr1), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Adgre1
• Synonyms: Emr1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR200880 representing NM_001007557
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGGGGCTTTTGGCTGCTCCTCTTCTGGGGCTTCAGTGGAACGCACAGATGGGGGATGACCACACTTG
  CCATCCTGGGACAAAGACTTAACGGTGTGAATGAATGTCAAGACACTACCACTTGTCCAGCTTATGCCAC
  CTGCACTGACACCACGGAGAGTTATTACTGTACCTGTAAACAAGGTTTTCTGCCCAGCAATGGACAAACC
  AACTTTCAGGGCCCAGGAGTGGAATGTCAAGATGTTAATGAATGTCTTAGAAGCGACTCGCCTTGTGGTT
  CTAACTCAGTCTGCACCAATATCCCAGGACGAGCCAGGTGCAGTTGTCTTAGTGGCTTCTCTTCTTCCGC
  TGGTGGAAGTTGGATCCTGGGAAGTCCGGGTCATTTTCTCTGCACAGATGTTGACGAGTGTCTGACAATT
  GGGATCTGCCCTAAGAATTCTAACTGCTCTAACTCTGTGGGGAGCTACAGCTGCACCTGCCAATCAGGCT
  TTGTCTCGAATGGCTCCACTTGTGAAGATGAAGATGAATGTGTGACTCGTAATGCTTGCCCAGAGCATGC
  AACCTGCCACAACACTCTTGGAAGCTATTATTGTACATGCAACGAAGGACTTGAATTTAGTGGAGGAGGA
  CCAATGTTCCAGGGCCTGGAAGAATCTTGTGAAGATGTGGATGAGTGCTCCAGGAATTCAACGCTTTGTG
  GTCCCTCCTTCATTTGCATCAACACGCTGGGATCCTACAGCTGTTCTTGCCCAGCTGGATTCTCTTTGTC
  AACTTTTCAGATCCCTGGTCATCCAGCAGATGGGAATTGTACAGATATAGATGAGTGTGATGACATATGC
  CCTTCCAACTCATCATGTACCAACACCCTTGGGAGCTACTTCTGCACTTGCCACCCTGGCTTTGCCTCAA
  GCAATGGACAGCTGAATTTCACAGACCAAGAAGTGACATGTGAAGATATTGATGAGTGCACTCAAGATCC
  ATTTCGATGTGGACGAAATTCTTCCTGCACCAATGTACCAGGTTCCTACAACTGCAGCTGTCTTCCCGAC
  TTTCGAATGGATCCAGGAGGCTCCCAAGCACATGGAAACTTCACATGCAAAAGGATTCCCTTCAAGTGTA
  AGGAAGACTTGATACCCAAAAGTGAGCAGATAGAGCAGTGCCAAGCAGGGCAAGGCCGGAATCTTGATTA
  TACTTCCTTCTGCACATTTGTGAATGCCACCTTTACCATCCTGGATAACACCTGTGAAAACAAAAGTGCT
  CCAGTGTCCTTACAGAGTGCAGCTACAAGTGTCTCCCTCATGCTGGAGCAAGCATCGACCTGGTTTGAGT
  TCAGCAGGGAAGAGACATCTACTCTGGGCACTATCTTGCTGGAGACTGTGGAAAGCACCATGTTGGCTGC
  TCTTCTGACACCCTCTGGGAATGCCAGTCAGACCATTCGGACGGAGTACTTGGAAATTGAAAGCAAAGTC
  ATCAATGAAGAATGCAATGAGGAGAATGTGTCCATTAACTTGAAAGCCAGAGGGGACAAGATGGATGTTG
  GCTGTTTCATAATCAAAGAATCAGAATCTACAGGGACCCCAGGAGTGGCTTTTGTCTCCTTTGCACACAT
  GGACTCAGTTTTAGATGAGCGCTTCTTTGAAGATGGTCAGGCCTCCTGGAAACTGAGGATGAATTCCCAT
  GTTGTTGGTGGCACTGTAACCGGGGAGAGGAAGGAGGACTTCTCCAAGCCTATCGTCTATACCCTCCAGC
  ACATCCAGCCAAAGCAGAAGTCTGAGAGGTCTATATGTGTCTCCTGGAACACCGATGTGGAGGATGGGAG
  ATGGACACCCTCGGGCTGTGAGACTGTAGAAGCATCTGAGACACACACAGTCTGCAGCTGTAACCGGATG
  ACAAATTTGGCCATCATCATGGCTTCTGGGGAGCTTACAATGGAATTTTCCTTGTATATCATCAGCTATG
  TGGGTACAGTCATCTCTCTGGTATGTCTCGCCTTGGCCATAGCCACCTTCCTGTTGTTTCGTGCAGTTCA
  GAACCACAACACCTACCTGCACCTGCACCTTTGTGTGTGTCTCTTCTTGGCCAAGATTCTCTTCCTCACC
  GGTATAGACAAGACTGACAACCAGACAGCTTGCGCTATCATTGCGGGATTTCTACACTATCTTTTCCTTG
  CCTGCTTCTTCTGGATGCTGGTGGAGGCAGTGATGCTCTTCCTGATGGTGAGAAACCTGAAGGTGGTGAA
  TTATTTCAGCTCTCGCAACATCAAGATGCTGCACCTCTGTGCCTTTGGCTATGGGCTCCCAGTCGTGGTG
  GTCATTATCTCTGCTACTGTACATCCATGGGGCTATGGAATGCATAATCGCTGCTGGCTGAATACGGAGA
  CAGGATTCATTTGGAGTTTCTTGGGGCCAGTCTGCATGATCATCACGATCAACTCTGCCCTCCTTGCCTG
  GACACTGTGGGTGCTGAGGCAGAAGCTCTGCAGTGTCAACTCAGAAGTCTCTAAGCTCAAGGATACGAGG
  TTGCTGACCTTCAAGGCCATTGCCCAGATTTTCATCTTGGGTTGCTCCTGGGTGCTGGGCATTTTCCAGA
  TTGGTCCCTTGGCAAGCATAATGGCATACCTGTTCACCACTATCAACAGCCTTCAGGGGGCCTTCATCTT
  CCTCATTCACTGTCTGCTCAACCGCCAGGTACGAGATGAATATAGGAAACTCCTCACCAGGAAGACTGAT
  CTTAGCTCCCATTCCCAGACTTCTGGGATTTTACTGTCATCTATGCCTTCCACTTCTAAGACGGGT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR200880 representing NM_001007557
  Red=Cloning site Green=Tags(s)
  
  MWGFWLLLFWGFSGTHRWGMTTLAILGQRLNGVNECQDTTTCPAYATCTDTTESYYCTCKQGFLPSNGQT
  NFQGPGVECQDVNECLRSDSPCGSNSVCTNIPGRARCSCLSGFSSSAGGSWILGSPGHFLCTDVDECLTI
  GICPKNSNCSNSVGSYSCTCQSGFVSNGSTCEDEDECVTRNACPEHATCHNTLGSYYCTCNEGLEFSGGG
  PMFQGLEESCEDVDECSRNSTLCGPSFICINTLGSYSCSCPAGFSLSTFQIPGHPADGNCTDIDECDDIC
  PSNSSCTNTLGSYFCTCHPGFASSNGQLNFTDQEVTCEDIDECTQDPFRCGRNSSCTNVPGSYNCSCLPD
  FRMDPGGSQAHGNFTCKRIPFKCKEDLIPKSEQIEQCQAGQGRNLDYTSFCTFVNATFTILDNTCENKSA
  PVSLQSAATSVSLMLEQASTWFEFSREETSTLGTILLETVESTMLAALLTPSGNASQTIRTEYLEIESKV
  INEECNEENVSINLKARGDKMDVGCFIIKESESTGTPGVAFVSFAHMDSVLDERFFEDGQASWKLRMNSH
  VVGGTVTGERKEDFSKPIVYTLQHIQPKQKSERSICVSWNTDVEDGRWTPSGCETVEASETHTVCSCNRM
  TNLAIIMASGELTMEFSLYIISYVGTVISLVCLALAIATFLLFRAVQNHNTYLHLHLCVCLFLAKILFLT
  GIDKTDNQTACAIIAGFLHYLFLACFFWMLVEAVMLFLMVRNLKVVNYFSSRNIKMLHLCAFGYGLPVVV
  VIISATVHPWGYGMHNRCWLNTETGFIWSFLGPVCMIITINSALLAWTLWVLRQKLCSVNSEVSKLKDTR
  LLTFKAIAQIFILGCSWVLGIFQIGPLASIMAYLFTTINSLQGAFIFLIHCLLNRQVRDEYRKLLTRKTD
  LSSHSQTSGILLSSMPSTSKTG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001007557
• ORF Size: 2796 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001007557.1, NP_001007558.1
• RefSeq Size: 3228 bp
• RefSeq ORF: 2799 bp
• Locus ID: 316137
• Cytogenetics: 9q12
• MW: 102.3 kDa