Dgkb (NM_019304) Rat Tagged ORF Clone

CAT#: RR200972

Dgkb (Myc-DDK-tagged ORF) - Rat diacylglycerol kinase, beta (Dgkb), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Dgkb
• Synonyms: Dagk
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR200972 representing NM_019304
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACAAACCAGGAAAAATGGGCCCATCTCAGCCCCTCAGAATTTTCCCAACTTCAGAAATATGCTGAGT
  ATTCTACAAAGAAATTAAAGGATGTTCTTGAAGAATTCCATGGTAATGGTGTGCTTGCAAAGTATAATCC
  TGAAGGGAAACAAGATATTCTTAACCAAACCATAGATTTTGAAGGATTCAAGCTGTTCATGAAGACCTTC
  CTGGAAGCTGAGCTCCCTGACGATTTCACTGCCCACCTTTTCATGTCATTCAGCAACAAATTCCCCCATT
  CCAGCCCCAACGTAAAGAGCAAGCCAGCTCTGCTTTCAGGTGGTTTGAGGATGAATAAGGGTGCCATCAC
  CCCTCCACGTTCTTCTCCAGCAAATACGTGCTTCCCGGAAGTGATCCACCTCAAGGATATTGTCTGTTAC
  CTGTCTCTTCTGGAAAGAGGAAGACCTGAGGACAAGCTTGAGTTTATGTTTCGCCTGTATGACACCGATG
  GGAATGGCTTCCTGGACAGCTCGGAATTAGAAAATATCATCGGTCAGATGATGCATGTTGCCGAGTACCT
  GGAATGGGATGTTACTGAACTTAATCCGATCCTTCATGAAATGATGGAAGAAATCGACTATGACCGTGAC
  GGCACTGTGTCTCTGGAAGAGTGGATTCAAGGAGGAATGACGACTATCCCACTTCTAGTCTTGCTGGGCT
  TGGAAAACAATGTGAAGGATGACGGACAGCATGTGTGGCGACTCAAGCACTTTAACAAGCCCGCCTACTG
  CAATCTCTGCCTGAACATGCTGATTGGCGTGGGGAAGCAGGGCCTCTGCTGCTCCTTCTGCAAGTACACC
  GTCCATGAGCGCTGTGCCAGAGCCCCGCCCTCCTGCATCAAGACCTATGTGAAGTCCAAGAAGAACACTG
  ACGTCATGCACCATTATTGGGTCGAAGGCAATTGCCCAACCAAGTGTGATAAGTGCCACAAGACAGTGAA
  ATGTTACCAGGGCCTGACGGGGCTGCACTGTGTTTGGTGTCAGACCACGCTGCACAATAAATGTGCTTCT
  CATCTGAAGCCTGAATGTGACTGTGGACCCTTGAAGGACCATATTTTGCCACCCACCACAATCTGTCCAG
  TTGTATTGACTATGCCCACTGCCGGAACTTCAGTTCCTGAGGAAAGACAGTCAACAGCTAAAAAGGAAAA
  GGGCAGCTCCCAGCAGCCAAATAAAGTGACAGACAAGAACAAAATGCAAAGAGCTAACTCTGTCACTATG
  GATGGACAAGGTTTGCAGATCACTCCTATTCCTGGCACACATCCTCTTCTAGTTTTTGTCAACCCTAAAA
  GTGGTGGGAAGCAAGGAGAACGAATTTACAGAAAATTCCAGTATCTTCTAAATCCTCGTCAGGTTTACAG
  TCTTTCTGGAAATGGACCAATGCCAGGGTTACATTTTTTTCGCGATGTCCCTGACTTCAGAGTGTTAGCA
  TGCGGTGGAGATGGGACTGTGGGCTGGATTTTGGACTGCATAGAAAAGGCCAATGTAGTCAAGCACCCTC
  CAGTTGCTATCCTGCCTCTTGGGACGGGCAATGACCTAGCAAGATGCCTGCGGTGGGGTGGAGGTTATGA
  AGGTGAAAATCTTATGAAGATCCTAAAAGACATTGAAAGCAGCACAGAAATCATGTTGGACAGGTGGAAG
  TTTGAAGTCACACCCAACGACAAAGACGAGAAAGGAGACCCTGTGCCTTACAGCATCATCAACAACTACT
  TTTCCATTGGCGTGGATGCTTCCATTGCACACAGATTCCACATCATGAGAGAAAAGCACCCGGAGAAATT
  CAACAGCCGAATGAAGAACAAATTTTGGTACTTTGAGTTTGGCACATCAGAAACCTTCTCAGCCACCTGC
  AAGAAACTGCATGAATCTGTAGAAATAGAATGTGATGGAGTACAGATAGATTTGATAAACATCTCTCTGC
  AAGGAATTGCAATTTTGAATATACCAAGCATGCATGGAGGATCTAATCTTTGGGGAGAATCTAAAAAGAA
  AAGAAGTCATCGACGGATAGAGAAAAAAGGCTCTGACAAAAGACCCACTCTCACAGATGCAAAAGAACTG
  AAGTTTGCAAGTCAAGATCTGAGTGACCAGCTACTGGAGGTTGTTGGCTTGGAAGGAGCAATGGAAATGG
  GCCAAATATACACGGGGCTGAAAAGTGCTGGCCGGCGGCTGGCTCAGTGCTCCTCTGTGGTTATCAGGAC
  AAGCAAGTCACTGCCAATGCAGATTGATGGAGAGCCATGGATGCAGACCCCATGCACAATAAAAATTACA
  CACAAGAACCAGGCCCCAATGCTGATGGGACCTCCACCTAAAACTGGATTATTCTGCTCCCTCATCAAAA
  GAACAAGAAACCGAAGCAAGGAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR200972 representing NM_019304
  Red=Cloning site Green=Tags(s)
  
  MTNQEKWAHLSPSEFSQLQKYAEYSTKKLKDVLEEFHGNGVLAKYNPEGKQDILNQTIDFEGFKLFMKTF
  LEAELPDDFTAHLFMSFSNKFPHSSPNVKSKPALLSGGLRMNKGAITPPRSSPANTCFPEVIHLKDIVCY
  LSLLERGRPEDKLEFMFRLYDTDGNGFLDSSELENIIGQMMHVAEYLEWDVTELNPILHEMMEEIDYDRD
  GTVSLEEWIQGGMTTIPLLVLLGLENNVKDDGQHVWRLKHFNKPAYCNLCLNMLIGVGKQGLCCSFCKYT
  VHERCARAPPSCIKTYVKSKKNTDVMHHYWVEGNCPTKCDKCHKTVKCYQGLTGLHCVWCQTTLHNKCAS
  HLKPECDCGPLKDHILPPTTICPVVLTMPTAGTSVPEERQSTAKKEKGSSQQPNKVTDKNKMQRANSVTM
  DGQGLQITPIPGTHPLLVFVNPKSGGKQGERIYRKFQYLLNPRQVYSLSGNGPMPGLHFFRDVPDFRVLA
  CGGDGTVGWILDCIEKANVVKHPPVAILPLGTGNDLARCLRWGGGYEGENLMKILKDIESSTEIMLDRWK
  FEVTPNDKDEKGDPVPYSIINNYFSIGVDASIAHRFHIMREKHPEKFNSRMKNKFWYFEFGTSETFSATC
  KKLHESVEIECDGVQIDLINISLQGIAILNIPSMHGGSNLWGESKKKRSHRRIEKKGSDKRPTLTDAKEL
  KFASQDLSDQLLEVVGLEGAMEMGQIYTGLKSAGRRLAQCSSVVIRTSKSLPMQIDGEPWMQTPCTIKIT
  HKNQAPMLMGPPPKTGLFCSLIKRTRNRSKE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_019304
• ORF Size: 2403 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_019304.1, NP_062177.1
• RefSeq Size: 6211
• RefSeq ORF: 2406
• Locus ID: 54248
• MW: 90.3 kDa
• Gene Summary: beta isotype of the diacylglycerol kinase family, regulates intracellular concentrations of the second messenger diacylglycerol (DAG) [RGD, Feb 2006]